DNA microextraction from dried blood spots on filter paper blotters: potential applications to newborn screening

1987 ◽  
Vol 75 (3) ◽  
pp. 213-216 ◽  
Author(s):  
Edward R. B. McCabe ◽  
Shu-Zhen Huang ◽  
William K. Seltzer ◽  
Martha L. Law
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Potential Applications

Gaucher and Niemann–Pick diseases—enzymatic diagnosis in dried blood spots on filter paper: retrospective diagnoses in newborn-screening cards

2002 ◽  
Vol 317 (1-2) ◽  
pp. 191-197 ◽  
Author(s):  
Néstor A. Chamoles ◽  
Mariana Blanco ◽  
Daniela Gaggioli ◽  
Carina Casentini
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Niemann Pick

scholarly journals Effect of Dried Blood Spot Quality on Newborn Screening Analyte Concentrations and Recommendations for Minimum Acceptance Criteria for Sample Analysis

2016 ◽  
Vol 62 (3) ◽  
pp. 466-475 ◽  
Author(s):  
Roanna S George ◽  
Stuart J Moat
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Statistical Significance ◽  
False Negative ◽  
Dried Blood Spots ◽  
Sample Analysis ◽  
Acceptance Criteria ◽  
Blood Spots ◽  
Spot Quality ◽  
Blood Spot

Abstract BACKGROUND The analysis of dried blood spots has been used routinely for newborn screening since the early 1970s, and the number of disorders screened has expanded substantially in recent years. However, there is a lack of evidence regarding minimum blood spot quality acceptance criteria for sample analysis. METHODS Blood pools were spiked with phenylalanine, tyrosine, leucine, methionine, octanoylcarnitine, decanoylcarnitine, isovalerylcarnitine, glutarylcarnitine, thyroid-stimulating hormone, and immunoreactive trypsinogen to concentrations at the analytical cutoffs used in UK screening protocols. We evaluated the effect of sample volume applied to the card (10, 20, 50, 75, and 100 μL), punch location (central vs peripheral), and sample quality (double layering, applying blood to both sides of the filter paper, multispotting, applying insufficient sample, and compressing the sample after application). RESULTS Compression of blood spots produced significantly lower results (14%–44%) for all analytes measured (P < 0.001). Smaller blood spots produced significantly lower results (15%–24% for 10-μL vs 50-μL sample size) for all analytes at all concentrations measured (P < 0.001). Results obtained from peripheral punches were higher than those from a central punch, although this did not reach statistical significance for all analytes. Insufficient and multispotted samples demonstrated heterogeneous results. CONCLUSIONS All blood spots containing ≤20 μL (blood spot diameter <8 mm), those in which blood has not fully penetrated the filter paper, and all samples with evidence of compression should be rejected, since there is a risk of producing false-negative results.

scholarly journals Effect of blood volume on analytical bias in dried blood spots prepared for newborn screening external quality assurance

Bioanalysis ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Stuart J Moat ◽  
Catherine Dibden ◽  
Lesley Tetlow ◽  
Caroline Griffith ◽  
Jim Chilcott ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Newborn Screening ◽  
Blood Volume ◽  
Filter Paper ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Measurement Bias ◽  
External Quality ◽  
Blood Spots ◽  
The Uk

Aim: Dried blood spots (DBS) are used for the analysis of more than 2000 biomarkers. We assessed a range of analyte concentrations and diameters of DBS. Materials & methods: DBS samples were created by the application of increasing volumes of whole blood prepared by the UK NEQAS Quality Assurance Laboratory. Samples were analyzed in four separate laboratories. Results: Volumes less than 25 μl (8 mm) and more than 75 μl (14 mm) created unsatisfactory analytical biases. Results obtained from peripheral subpunches tended to be higher than those from a central subpunch. Conclusion: DBS diameters formed from nonvolumetric application of blood to filter paper can be used to assess whether measurement bias will be within acceptable limits according to the analyte being quantified. DBS received for newborn screening in the UK with diameters less than 8 mm and those more than 14 mm should be rejected.

Tay-Sachs and Sandhoff diseases: enzymatic diagnosis in dried blood spots on filter paper: retrospective diagnoses in newborn-screening cards

2002 ◽  
Vol 318 (1-2) ◽  
pp. 133-137 ◽  
Author(s):  
Néstor A Chamoles ◽  
Mariana Blanco ◽  
Daniela Gaggioli ◽  
Carina Casentini
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

scholarly journals Utility of measuring very long-chain fatty-acyl carnitines in dried blood spots for newborn screening of X-linked Adrenoleukodystrophy

2021 ◽  
Vol 26 ◽  
pp. 100720
Author(s):  
Archana Natarajan ◽  
Rita Christopher ◽  
Shruti V. Palakuzhiyil ◽  
Sadanandavalli Retnaswami Chandra
Keyword(s):  
Newborn Screening ◽  
Dried Blood Spots ◽  
Fatty Acyl ◽  
Blood Spots ◽  
Long Chain

Alpha- l -iduronidase and arylsulfatase B in dried blood spots on filter paper: Biochemical parameters and time stability

2017 ◽  
Vol 50 (7-8) ◽  
pp. 431-435 ◽  
Author(s):  
Ana Carolina Breier ◽  
Jaqueline Cé ◽  
Jamila Mezzalira ◽  
Vanessa V. Daitx ◽  
Vitoria C. Moraes ◽  
...  
Keyword(s):  
Filter Paper ◽  
Biochemical Parameters ◽  
Dried Blood Spots ◽  
Time Stability ◽  
Blood Spots ◽  
Arylsulfatase B

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1

scholarly journals State of the Science in Dried Blood Spots

2018 ◽  
Vol 64 (4) ◽  
pp. 656-679 ◽  
Author(s):  
Jeffrey D Freeman ◽  
Lori M Rosman ◽  
Jeremy D Ratcliff ◽  
Paul T Strickland ◽  
David R Graham ◽  
...  
Keyword(s):  
Systematic Approach ◽  
Full Range ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Highly Sensitive ◽  
Wide Range ◽  
Potential Applications ◽  
Review Of Reviews ◽  
Near Term ◽  
State Of The Science

Abstract BACKGROUND Advancements in the quality and availability of highly sensitive analytical instrumentation and methodologies have led to increased interest in the use of microsamples. Among microsamples, dried blood spots (DBS) are the most well-known. Although there have been a variety of review papers published on DBS, there has been no attempt at describing the full range of analytes measurable in DBS, or any systematic approach published for characterizing the strengths and weaknesses associated with adoption of DBS analyses. CONTENT A scoping review of reviews methodology was used for characterizing the state of the science in DBS. We identified 2018 analytes measured in DBS and found every common analytic method applied to traditional liquid samples had been applied to DBS samples. Analytes covered a broad range of biomarkers that included genes, transcripts, proteins, and metabolites. Strengths of DBS enable its application in most clinical and laboratory settings, and the removal of phlebotomy and the need for refrigeration have expanded biosampling to hard-to-reach and vulnerable populations. Weaknesses may limit adoption in the near term because DBS is a nontraditional sample often requiring conversion of measurements to plasma or serum values. Opportunities presented by novel methodologies may obviate many of the current limitations, but threats around the ethical use of residual samples must be considered by potential adopters. SUMMARY DBS provide a wide range of potential applications that extend beyond the reach of traditional samples. Current limitations are serious but not intractable. Technological advancements will likely continue to minimize constraints around DBS adoption.

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