Surface plasmon resonance as a tool for ligand-binding assay reagent characterization in bioanalysis of biotherapeutics

Bioanalysis ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 559-576 ◽  
Author(s):  
Jia Duo ◽  
JoAnne Bruno ◽  
Alexander Kozhich ◽  
Donata David-brown ◽  
Linlin Luo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Reagent Characterization

scholarly journals Interaction of human fibronectin with Candida glabrata epithelial adhesin 6 (Epa6)

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Dorota Zajac ◽  
Justyna Karkowska-Kuleta ◽  
Oliwia Bochenska ◽  
Maria Rapala-Kozik ◽  
Andrzej Kozik
Keyword(s):  
Extracellular Matrix ◽  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assay ◽  
Host Protein ◽  
Human Organism ◽  
Ligand Binding Assay ◽  
Human Fibronectin

Adherence of pathogens to extracellular matrix proteins and host cells is one of the essential steps in the microbial colonization of the human organism. The adhesion of  C. glabrata, i.e. the second major causative agent of human disseminated candidiases after C. albicans, to the host epithelium mainly engages specific fungal cell wall proteins—epithelial adhesins (Epa)—in particular, Epa1, Epa6 and Epa7. The aim of the present study was to identify the major Epa protein involved in the interactions with the human extracellular matrix protein—fibronectin—and to present the kinetic and thermodynamic characteristics of these interactions. A relatively novel gel-free approach, i.e. the “cell surface shaving” that consists in short treatment of fungal cells with trypsin was employed to identify the C. glabrata surfaceome. Epa6 was purified, and the isolated protein was characterized in terms of its affinity to human fibronectin using a microplate ligand-binding assay and surface plasmon resonance measurements. The dissociation constants for the binding of Epa6 to fibronectin were determined to range between 9.03 × 10–9 M and 7.22 × 10–8 M, depending on the method used (surface plasmon resonance measurements versus the microplate ligand-binding assay, respectively). The identified fungal pathogen-human host protein-protein interactions might become a potential target for novel anticandidal therapeutic approaches.

Characterization of labeled reagents in ligand-binding assays by a surface plasmon resonance biosensor

Bioanalysis ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 193-207 ◽  
Author(s):  
Jia Duo ◽  
JoAnne Bruno ◽  
Steven Piccoli ◽  
Binodh DeSilva ◽  
Yan J Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assays ◽  
Surface Plasmon Resonance Biosensor

scholarly journals Analysis by Surface Plasmon Resonance of the Influence of Valence on the Ligand Binding Affinity and Kinetics of an Anti-carbohydrate Antibody

1996 ◽  
Vol 271 (3) ◽  
pp. 1527-1533 ◽  
Author(s):  
C. Roger MacKenzie ◽  
Tomoko Hirama ◽  
Su-jun Deng ◽  
David R. Bundle ◽  
Saran A. Narang ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Binding Affinity ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Kinetics Of

Development and Applications of Surface Plasmon Resonance-Based von Willebrand Factor– Collagen Binding Assay

2002 ◽  
Vol 302 (2) ◽  
pp. 252-262 ◽  
Author(s):  
Evgueni Saenko ◽  
Christoph Kannicht ◽  
Klemens Loster ◽  
Andrey Sarafanov ◽  
Alexey Khrenov ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Von Willebrand Factor ◽  
Plasmon Resonance ◽  
Binding Assay ◽  
Collagen Binding ◽  
Von Willebrand ◽  
Willebrand Factor

Development and optimization of a binding assay for histone deacetylase 4 using surface plasmon resonance

2008 ◽  
Vol 377 (2) ◽  
pp. 267-269 ◽  
Author(s):  
Marco Mattu ◽  
Paolo Di Giovine ◽  
Christian Steinkuhler ◽  
Raffaele De Francesco ◽  
Sergio Altamura ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Histone Deacetylase ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assay ◽  
Histone Deacetylase 4
Sign in / Sign up

Export Citation Format

Share Document