scholarly journals Development of a label-free competitive ligand binding assay with human serum albumin on a molecularly engineered surface plasmon resonance sensor chip

2012 ◽  
Vol 4 (11) ◽  
pp. 3718 ◽  
Author(s):  
Yu Gao ◽  
Xinxin Li ◽  
Liang-Hong Guo
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum Albumin ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Surface Plasmon Resonance Sensor ◽  
Label Free ◽  
Resonance Sensor ◽  
Competitive Ligand

scholarly journals Interaction of human fibronectin with Candida glabrata epithelial adhesin 6 (Epa6)

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Dorota Zajac ◽  
Justyna Karkowska-Kuleta ◽  
Oliwia Bochenska ◽  
Maria Rapala-Kozik ◽  
Andrzej Kozik
Keyword(s):  
Extracellular Matrix ◽  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assay ◽  
Host Protein ◽  
Human Organism ◽  
Ligand Binding Assay ◽  
Human Fibronectin

Adherence of pathogens to extracellular matrix proteins and host cells is one of the essential steps in the microbial colonization of the human organism. The adhesion of  C. glabrata, i.e. the second major causative agent of human disseminated candidiases after C. albicans, to the host epithelium mainly engages specific fungal cell wall proteins—epithelial adhesins (Epa)—in particular, Epa1, Epa6 and Epa7. The aim of the present study was to identify the major Epa protein involved in the interactions with the human extracellular matrix protein—fibronectin—and to present the kinetic and thermodynamic characteristics of these interactions. A relatively novel gel-free approach, i.e. the “cell surface shaving” that consists in short treatment of fungal cells with trypsin was employed to identify the C. glabrata surfaceome. Epa6 was purified, and the isolated protein was characterized in terms of its affinity to human fibronectin using a microplate ligand-binding assay and surface plasmon resonance measurements. The dissociation constants for the binding of Epa6 to fibronectin were determined to range between 9.03 × 10–9 M and 7.22 × 10–8 M, depending on the method used (surface plasmon resonance measurements versus the microplate ligand-binding assay, respectively). The identified fungal pathogen-human host protein-protein interactions might become a potential target for novel anticandidal therapeutic approaches.

Surface plasmon resonance as a tool for ligand-binding assay reagent characterization in bioanalysis of biotherapeutics

Bioanalysis ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 559-576 ◽  
Author(s):  
Jia Duo ◽  
JoAnne Bruno ◽  
Alexander Kozhich ◽  
Donata David-brown ◽  
Linlin Luo ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Ligand Binding ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Reagent Characterization

TBG MEASUREMENTS BY COMPETITIVE LIGAND BINDING ASSAY (CLBA)

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S15
Author(s):  
K. H. Rudorff ◽  
H. J. Kröll ◽  
J. Herrmann
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Competitive Ligand

Dual-mode surface plasmon resonance sensor chip using a grating 3D-printed prism

2021 ◽  
Vol 1147 ◽  
pp. 23-29
Author(s):  
Chutiparn Lertvachirapaiboon ◽  
Akira Baba ◽  
Kazunari Shinbo ◽  
Keizo Kato
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Sensor Chip ◽  
Surface Plasmon Resonance Sensor ◽  
Dual Mode ◽  
Resonance Sensor ◽  
3D Printed

scholarly journals Nanocrystalline TiO2 Sensitive Layer for Plasmonic Hydrogen Sensing

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1490
Author(s):  
Enrico Gazzola ◽  
Michela Cittadini ◽  
Marco Angiola ◽  
Laura Brigo ◽  
Massimo Guglielmi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon ◽  
Surface Plasmon Resonance Sensor ◽  
Sensitive Layer ◽  
Plasmonic Sensor ◽  
Nanocrystalline Tio2 ◽  
Resonance Sensor

Solution processed TiO2 anatase film was used as sensitive layer for H2 detection for two plasmonic sensor configurations: A grating-coupled surface plasmon resonance sensor and a localized surface plasmon resonance sensor with gold nanoparticles. The main purpose of this paper is to elucidate the different H2 response observed for the two types of sensors which can be explained considering the hydrogen dissociation taking place on TiO2 at high temperature and the photocatalytic activity of the gold nanoparticles.

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