‘Omics’ technologies and systems biology for engineeringSaccharomyces cerevisiaestrains for lignocellulosic bioethanol production

Biofuels ◽  
2011 ◽  
Vol 2 (6) ◽  
pp. 659-675 ◽  
Author(s):  
Dominic Pinel ◽  
Pratish Gawand ◽  
Radhakrishnan Mahadevan ◽  
Vincent JJ Martin
2018 ◽  
Author(s):  
Milan Martinov ◽  
Peter Schulze Lammers ◽  
Djordje Djatkov ◽  
Miodrag Viskovic

2014 ◽  
Vol 1 (4) ◽  
pp. 311-321 ◽  
Author(s):  
I. Henaut ◽  
F. Ben Chaabane ◽  
N. Lopes Ferreira ◽  
F. Augier

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Seonghun Kim

Soybean straw is a renewable resource in agricultural residues that can be used for lignocellulosic bioethanol production. To enhance enzymatic digestibility and fermentability, the biomass was prepared with an alkali-thermal pretreatment (sodium hydroxide, 121°C, 60 min). The delignification yield was 34.1~53%, in proportion to the amount of sodium hydroxide, from 0.5 to 3.0 M. The lignin and hemicellulose contents of the pretreated biomass were reduced by the pretreatment process, whereas the proportion of cellulose was increased. Under optimal condition, the pretreated biomass consisted of 74.0±0.1% cellulose, 10.3±0.1% hemicellulose, and 10.1±0.6% lignin. During enzymatic saccharification using Cellic® CTec2 cellulase, 10% (w/v) of pretreated soybean straw was hydrolyzed completely and converted to 67.3±2.1 g/L glucose and 9.4±0.5 g/L xylose with a 90.9% yield efficiency. Simultaneous saccharification and fermentation of the pretreated biomass by Saccharomyces cerevisiae W303-1A produced 30.5±1.2 g/L ethanol in 0.5 L fermented medium containing 10% (w/v) pretreated biomass after 72 h. The ethanol productivity was 0.305 g ethanol/g dry biomass and 0.45 g ethanol/g glucose after fermentation, with a low concentration of organic acid metabolites. Also, 82% of fermentable sugar was used by the yeast for ethanol fermentation. These results show that the combination of alkaline pretreatment and biomass hydrolysate is useful for enhancing bioethanol productivity using delignified soybean straw.


Author(s):  
Bashar Amer ◽  
Edward E. K. Baidoo

Biomanufacturing is a key component of biotechnology that uses biological systems to produce bioproducts of commercial relevance, which are of great interest to the energy, material, pharmaceutical, food, and agriculture industries. Biotechnology-based approaches, such as synthetic biology and metabolic engineering are heavily reliant on “omics” driven systems biology to characterize and understand metabolic networks. Knowledge gained from systems biology experiments aid the development of synthetic biology tools and the advancement of metabolic engineering studies toward establishing robust industrial biomanufacturing platforms. In this review, we discuss recent advances in “omics” technologies, compare the pros and cons of the different “omics” technologies, and discuss the necessary requirements for carrying out multi-omics experiments. We highlight the influence of “omics” technologies on the production of biofuels and bioproducts by metabolic engineering. Finally, we discuss the application of “omics” technologies to agricultural and food biotechnology, and review the impact of “omics” on current COVID-19 research.


2014 ◽  
Vol 35 (3) ◽  
pp. 342-354 ◽  
Author(s):  
Antonio D. Moreno ◽  
David Ibarra ◽  
Pablo Alvira ◽  
Elia Tomás-Pejó ◽  
Mercedes Ballesteros

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