scholarly journals Evaluation of Alkali-Pretreated Soybean Straw for Lignocellulosic Bioethanol Production

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Seonghun Kim
Keyword(s):  
Sodium Hydroxide ◽  
Enzymatic Saccharification ◽  
Renewable Resource ◽  
Bioethanol Production ◽  
Yield Efficiency ◽  
Lignocellulosic Bioethanol ◽  
Acid Metabolites ◽  
Soybean Straw ◽  
Pretreated Biomass ◽  
Simultaneous Saccharification

Soybean straw is a renewable resource in agricultural residues that can be used for lignocellulosic bioethanol production. To enhance enzymatic digestibility and fermentability, the biomass was prepared with an alkali-thermal pretreatment (sodium hydroxide, 121°C, 60 min). The delignification yield was 34.1~53%, in proportion to the amount of sodium hydroxide, from 0.5 to 3.0 M. The lignin and hemicellulose contents of the pretreated biomass were reduced by the pretreatment process, whereas the proportion of cellulose was increased. Under optimal condition, the pretreated biomass consisted of 74.0±0.1% cellulose, 10.3±0.1% hemicellulose, and 10.1±0.6% lignin. During enzymatic saccharification using Cellic® CTec2 cellulase, 10% (w/v) of pretreated soybean straw was hydrolyzed completely and converted to 67.3±2.1 g/L glucose and 9.4±0.5 g/L xylose with a 90.9% yield efficiency. Simultaneous saccharification and fermentation of the pretreated biomass by Saccharomyces cerevisiae W303-1A produced 30.5±1.2 g/L ethanol in 0.5 L fermented medium containing 10% (w/v) pretreated biomass after 72 h. The ethanol productivity was 0.305 g ethanol/g dry biomass and 0.45 g ethanol/g glucose after fermentation, with a low concentration of organic acid metabolites. Also, 82% of fermentable sugar was used by the yeast for ethanol fermentation. These results show that the combination of alkaline pretreatment and biomass hydrolysate is useful for enhancing bioethanol productivity using delignified soybean straw.

scholarly journals Enhancing Bioethanol Productivity Using Alkali-Pretreated Empty Palm Fruit Bunch Fiber Hydrolysate

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Seonghun Kim
Keyword(s):  
Sodium Hydroxide ◽  
Batch Fermentation ◽  
Ethanol Yield ◽  
Lignin Content ◽  
Enzymatic Saccharification ◽  
Renewable Resource ◽  
Palm Fruit ◽  
Fed Batch Fermentation ◽  
High Lignin Content ◽  
Simultaneous Saccharification

Empty palm fruit bunch fiber (EPFBF) is a renewable resource in oil palm plantations that can be used for lignocellulosic bioethanol production. To enhance ethanol productivity with high-lignin-content EPFBF, the biomass was prepared with an alkali-thermal pretreatment (sodium hydroxide, 121°C, 60 min). The delignification yield was 55.4–56.9%, in proportion to the amount of sodium hydroxide, from 0.5 to 2.0 M. The lignin and hemicellulose contents of EPFBF were reduced by the pretreatment process, whereas the proportion of cellulose was increased. During enzymatic saccharification using Celluclast 1.5L and Novozyme 188 enzyme cocktails, about 62% of glucan was converted to a fermentable sugar. In simultaneous saccharification and fermentation, comparison among three ethanologenic yeast strains showed Saccharomyces cerevisiae W303-1A to be a candidate for maximum ethanol yield. In a batch fermentation with alkali-pretreated EPFBF hydrolysate, 21 g/L ethanol was obtained within 28 h, for a production yield of 0.102 g ethanol/g dry EPFBF or 0.458 g ethanol/g glucose. Moreover, a fed-batch fermentation produced 33.8±0.5 g/L ethanol with 1.57 g/L/h productivity in 20 h. These results show that the combination of alkaline pretreatment and biomass hydrolysate is useful for enhancing bioethanol productivity using delignified EPFBF.

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

scholarly journals Simultaneous Saccharification and Fermentation of Pine Sawdust (Pinus pseudostrobus L.) Pretreated with Nitric Acid and Sodium Hydroxide for Bioethanol Production

BioResources ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Juan Carlos Farías-Sánchez ◽  
Ulises Velázquez-Valadez ◽  
María Guadalupe Pineda-Pimentel ◽  
Javier López-Miranda ◽  
Agustín Jaime Castro-Montoya ◽  
...  
Keyword(s):  
Nitric Acid ◽  
Sodium Hydroxide ◽  
Simultaneous Saccharification And Fermentation ◽  
Bioethanol Production ◽  
Pine Sawdust ◽  
Pinus Pseudostrobus ◽  
Simultaneous Saccharification

scholarly journals Application of Pichia kudriavzevii NBRC1279 and NBRC1664 to Simultaneous Saccharification and Fermentation for Bioethanol Production

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 83
Author(s):  
Hironaga Akita ◽  
Tetsuya Goshima ◽  
Toshihiro Suzuki ◽  
Yuya Itoiri ◽  
Zen-ichiro Kimura ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Draft Genome ◽  
Enzymatic Saccharification ◽  
Japanese Cedar ◽  
Biofuel Production ◽  
Venn Diagram ◽  
Genome Comparison ◽  
Bioethanol Production ◽  
Pichia Kudriavzevii ◽  
Simultaneous Saccharification

Simultaneous saccharification and fermentation (SSF) is capable of performing enzymatic saccharification and fermentation for biofuel production in a single vessel. Thus, SSF has several advantages such as simplifying the manufacturing process, operating easily, and reducing energy input. Here, we describe the application of Pichia kudriavzevii NBRC1279 and NBRC1664 to SSF for bioethanol production. When each strain was incubated for 144 h at 35 °C with Japanese cedar particles, the highest ethanol concentrations were reached 21.9 ± 0.50 g/L and 23.8 ± 3.9 g/L, respectively. In addition, 21.6 ± 0.29 g/L and 21.3 ± 0.21 g/L of bioethanol were produced from Japanese eucalyptus particles when each strain was incubated for 144 h at 30 °C. Although previous methods require pretreatment of the source material, our method does not require pretreatment, which is an advantage for industrial use. To elucidate the different characteristics of the strains, we performed genome sequencing and genome comparison. Based on the results of the eggNOG categories and the resulting Venn diagram, the functional abilities of both strains were similar. However, strain NBRC1279 showed five retrotransposon protein genes in the draft genome sequence, which indicated that the stress tolerance of both strains is slightly different.

scholarly journals Delignification of Cistus ladanifer Biomass by Organosolv and Alkali Processes

Energies ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 1127
Author(s):  
Júnia Alves-Ferreira ◽  
Ana Lourenço ◽  
Francisca Morgado ◽  
Luís C. Duarte ◽  
Luísa B. Roseiro ◽  
...  
Keyword(s):  
Steam Distillation ◽  
Enzymatic Saccharification ◽  
Added Value ◽  
Cistus Ladanifer ◽  
Reaction Conditions ◽  
Zone Electrophoresis ◽  
Monomeric Composition ◽  
Capillary Zone ◽  
Soluble Lignin ◽  
Pretreated Biomass

Residues of Cistus ladanifer obtained after commercial steam distillation for essential oil production were evaluated to produce cellulose enriched solids and added-value lignin-derived compounds. The delignification of extracted (CLRext) and extracted and hydrothermally pretreated biomass (CLRtreat) was studied using two organosolv processes, ethanol/water mixtures (EO), and alkali-catalyzed glycerol (AGO), and by an alkali (sodium hydroxide) process (ASP) under different reaction conditions. The phenolic composition of soluble lignin was determined by capillary zone electrophoresis and by Py-GC/MS, which was also used to establish the monomeric composition of both the delignified solids and isolated lignin. The enzymatic saccharification of the delignified solids was also evaluated. The ASP (4% NaOH, 2 h) lead to both the highest delignification and enzymatic saccharification (87% and 79%, respectively). A delignification of 76% and enzymatic hydrolysis yields of 72% were obtained for AGO (4% NaOH) while EO processes led to lower delignification (maximum lignin removal 29%). The residual lignin in the delignified solids were enriched in G- and H-units, with S-units being preferentially removed. The main phenolics present in the ASP and AGO liquors were vanillic acid and epicatechin, while gallic acid was the main phenolic in the EO liquors. The results showed that C. ladanifer residues can be a biomass source for the production of lignin-derivatives and glucan-rich solids to be further used in bioconversion processes.

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