scholarly journals The possible role of NS3 protease activity of hepatitis C virus on fibrogenesis and miR-122 expression in hepatic stellate cells

2016 ◽  
Vol 60 (03) ◽  
pp. 242-248 ◽  
Author(s):  
S. KHANIZADEH ◽  
M. RAVANSHAD ◽  
S. Y. HOSSEINI ◽  
P. DAVOODIAN ◽  
A. N. ZADEH ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatic Stellate Cells ◽  
Protease Activity ◽  
Stellate Cells ◽  
Ns3 Protease

1046 ROLE OF ANGIOPOIETIN RECEPTOR TIE-2 IN THE INVASION OF HUMAN HEPATIC STELLATE CELLS INDUCED BY HEPATITIS C VIRUS

2011 ◽  
Vol 54 ◽  
pp. S415-S416
Author(s):  
S. Martín-Vílchez ◽  
Y. Rodríguez-Muñoz ◽  
R. López-Rodríguez ◽  
Á. Hernández-Bartolomé ◽  
F. Molina-Jiménez ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatic Stellate Cells ◽  
Stellate Cells

scholarly journals Hepatitis C Virus-Infected Apoptotic Hepatocytes Program Macrophages and Hepatic Stellate Cells for Liver Inflammation and Fibrosis Development: Role of Ethanol as a Second Hit

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 113 ◽  
Author(s):  
Murali Ganesan ◽  
Larisa Poluektova ◽  
Chijioke Enweluzo ◽  
Kusum Kharbanda ◽  
Natalia Osna
Keyword(s):  
Lipid Peroxidation ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatic Stellate Cells ◽  
Parenchymal Cell ◽  
Human Monocyte ◽  
Stellate Cells ◽  
Liver Inflammation ◽  
Parenchymal Cells

Hepatocyte apoptosis is a crucially important mechanism for liver disease pathogenesis, and the engulfment of apoptotic bodies (AB) by non-parenchymal cells serves as a leading mechanism of inflammation and fibrosis progression. Previously, we have shown that hepatitis C virus (HCV) and alcohol metabolites induce massive apoptosis in hepatocytes and the spread of HCV-infection to the neighboring uninfected cells. Here, we hypothesize that the capturing of AB by non-parenchymal cells, macrophages and hepatic stellate cells (HSC) changes their phenotype to promote inflammation and fibrosis. In this regard, we generated AB from Huh7.5CYP2E1 (RLW) cells also treated with an acetaldehyde-generating system (AGS) and incubated them with human monocyte-derived macrophages (MDMs) and HSC (LX2 cells). Activation of inflammasomes and pro-fibrotic markers has been tested by RT-PCR and linked to HCV expression and AGS-induced lipid peroxidation in RLW cells. After exposure to AB we observed activation of inflammasomes in MDMs, with a higher effect of AB HCV+, further enhanced by incubation of MDMs with ethanol. In HSC, activation of inflammasomes was modest; however, HCV and AGS exposure induced pro-fibrotic changes. We conclude that HCV as well as lipid peroxidation-adducted proteins packaged in AB may serve as a vehicle for delivery of parenchymal cell cargo to non-parenchymal cells to activate inflammasomes and pro-fibrotic genes and promote liver inflammation and fibrosis.

scholarly journals Hepatitis C virus-induced CCL5 secretion from macrophages activates hepatic stellate cells

Hepatology ◽  
2017 ◽  
Vol 66 (3) ◽  
pp. 746-757 ◽  
Author(s):  
Reina Sasaki ◽  
Pradip B. Devhare ◽  
Robert Steele ◽  
Ranjit Ray ◽  
Ratna B. Ray
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatic Stellate Cells ◽  
Stellate Cells

scholarly journals The Role of Signal Transducer and Activator of Transcription 5 and Transforming Growth Factor-β1 in Hepatic Fibrosis Induced by Chronic Hepatitis C Virus Infection in Egyptian Patients

2018 ◽  
Vol 27 (2) ◽  
pp. 115-121
Author(s):  
Mona A. Abu El Makarem ◽  
Ghada M. El-Sagheer ◽  
Moustafa A. Abu El-Ella
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Liver Fibrosis ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Serum Levels ◽  
Signal Transducer ◽  
Egyptian Patients ◽  
Tgf Β1

Objective: To investigate the possible role of signal transducer and activator of transcription 5 (STAT5) in the pathogenesis of liver fibrosis in Egyptian patients with chronic hepatitis C (CHC) virus infection and its relation to hepatic stellate cells (HSC). Subjects and Methods: Sixty-five patients (46 males and 19 females) were divided into 4 groups based on the severity of fibrosis as detected by Fibroscan as follows: F1, n = 15; F2, n = 21; F3, n = 13; and F4, n = 16. Twenty age- and gender-matched healthy persons volunteered as controls. The serum levels of STAT5, TGF-β1, α-smooth muscle actin (α-SMA), fasting blood sugar, and fasting insulin, as well as homeostasis model assessment of insulin resistance (HOMA-IR), were determined and compared for all groups. The usefulness of the studied serum biomarkers for predicting liver fibrosis was evaluated using a receiver operating characteristic curve. Results: Serum levels of STAT5 were significantly lower in patients compared to controls (9.69 ± 5.62 vs. 14.73 ± 6.52, p ≤ 0.001); on the contrary, TGF-β1, α-SMA, and HOMA-IR were significantly higher in patients compared to controls (mean: 1,796.04 vs. 1,636.94; 14.94 vs. 8.1; and 7.91 vs. 4.18; p ≤ 0.01 and 0.001, respectively). TGF-β1 and α-SMA showed a progressive increase with advancing severity of hepatic fibrosis (mean TGF-β1: 2,058.4 in F1-F2 and 1,583.8 in F3-F4, p ≤ 0.04; mean α-SMA: 13.59 in F1-F2 and 16.62 in F3-F4, p ≤ 0.05). STAT5 had a significant negative correlation with TGF-β1 (p ≤ 0.001), while no correlation was detected with α-SMA (p ≤ 0.8). Conclusions: STAT5 may play a significant role in hepatic fibrogenesis through the induction of TGF-β1 but not through the activation of hepatic stellate cells.

scholarly journals Hepatitis C virus core protein induces fibrogenic actions of hepatic stellate cells via toll-like receptor 2

2011 ◽  
Vol 91 (9) ◽  
pp. 1375-1382 ◽  
Author(s):  
Martin Coenen ◽  
Hans Dieter Nischalke ◽  
Benjamin Krämer ◽  
Bettina Langhans ◽  
Andreas Glässner ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatic Stellate Cells ◽  
Core Protein ◽  
Stellate Cells ◽  
Toll Like Receptor ◽  
Virus Core ◽  
Toll Like Receptor 2

Hepatic Stellate Cells and Fibrogenesis in Hepatitis C Virus Infection: An Ultrastructural Insight

2010 ◽  
Vol 34 (2) ◽  
pp. 62-67 ◽  
Author(s):  
Soheir S. Mansy ◽  
Nagwa A. ElKhafif ◽  
Ahmed S. AbelFatah ◽  
Hoda A. Yehia ◽  
Ibrahim Mostafa
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Hepatitis C Virus Infection

386 PARACRINE ACTIVATION OF HUMAN HEPATIC STELLATE CELLS BY HEPATITIS C VIRUS VIA ANGIOPOIETIN RECEPTOR, TIE-2

2012 ◽  
Vol 56 ◽  
pp. S155
Author(s):  
S. Martin-Vilchez ◽  
Y. Rodriguez-Muñoz ◽  
R. Lopez-Rodriguez ◽  
A. Bartolome-Hernandez ◽  
R. Aldabe ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Paracrine Activation
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