scholarly journals Fusion of HSP70 gene of Mycobacterium tuberculosis to hemagglutinin (H5) gene of avian influenza virus in DNA vaccine enhances its potency

2010 ◽  
Vol 54 (1) ◽  
pp. 33-39 ◽  
Author(s):  
M. Rasoli ◽  
A. Omar ◽  
I. Aini ◽  
B. Jalilian ◽  
S. Syed_Hassan ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Mycobacterium Tuberculosis ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Dna Vaccine ◽  
Hsp70 Gene ◽  
Hemagglutinin H5

scholarly journals DNA Vaccine Encoding Hemagglutinin Provides Protective Immunity against H5N1 Influenza Virus Infection in Mice

1999 ◽  
Vol 73 (3) ◽  
pp. 2094-2098 ◽  
Author(s):  
Shantha Kodihalli ◽  
Hideo Goto ◽  
Darwyn L. Kobasa ◽  
Scott Krauss ◽  
Yoshihiro Kawaoka ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Dna Vaccine ◽  
Influenza Virus Infection ◽  
Glycosylation Site ◽  
Mammalian Host ◽  
H5n1 Infection ◽  
H5n1 Avian Influenza

ABSTRACT In Hong Kong in 1997, a highly lethal H5N1 avian influenza virus was apparently transmitted directly from chickens to humans with no intermediate mammalian host and caused 18 confirmed infections and six deaths. Strategies must be developed to deal with this virus if it should reappear, and prospective vaccines must be developed to anticipate a future pandemic. We have determined that unadapted H5N1 viruses are pathogenic in mice, which provides a well-defined mammalian system for immunological studies of lethal avian influenza virus infection. We report that a DNA vaccine encoding hemagglutinin from the index human influenza isolate A/HK/156/97 provides immunity against H5N1 infection of mice. This immunity was induced against both the homologous A/HK/156/97 (H5N1) virus, which has no glycosylation site at residue 154, and chicken isolate A/Ck/HK/258/97 (H5N1), which does have a glycosylation site at residue 154. The mouse model system should allow rapid evaluation of the vaccine’s protective efficacy in a mammalian host. In our previous study using an avian model, DNA encoding hemagglutinin conferred protection against challenge with antigenic variants that differed from the primary antigen by 11 to 13% in the HA1 region. However, in our current study we found that a DNA vaccine encoding the hemagglutinin from A/Ty/Ir/1/83 (H5N8), which differs from A/HK/156/97 (H5N1) by 12% in HA1, prevented death but not H5N1 infection in mice. Therefore, a DNA vaccine made with a heterologous H5 strain did not prevent infection by H5N1 avian influenza viruses in mice but was useful in preventing death.

scholarly journals Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules.

2014 ◽  
Vol 61 (3) ◽  
Author(s):  
Anna Stachyra ◽  
Anna Góra-Sochacka ◽  
Włodzimierz Zagórski-Ostoja ◽  
Ewelina Król ◽  
Agnieszka Sirko
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Dna Vaccine ◽  
Humoral Response ◽  
Cross Reactivity ◽  
Control Group ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
High Level

Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.

scholarly journals Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant

2010 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Babak Jalilian ◽  
Abdul Omar ◽  
Mohd Bejo ◽  
Noorjahan Alitheen ◽  
Mehdi Rasoli ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Dna Vaccine ◽  
Mycobacterium Bovis

scholarly journals Stable lentiviral transformation of CHO cells for the expression of the hemagglutinin H5 of avian influenza virus in suspension culture

2014 ◽  
Vol 3 ◽  
pp. 108-116 ◽  
Author(s):  
Alaín González Pose ◽  
Nayrobis Oramas Morell ◽  
Dailenis Abella Matos ◽  
Elsa Rodríguez Rodríguez ◽  
Elaine Santana Rodríguez ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Suspension Culture ◽  
Avian Influenza Virus ◽  
Cho Cells ◽  
Hemagglutinin H5
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