scholarly journals Induction of a robust immune response against avian influenza virus following transdermal inoculation with H5-DNA vaccine formulated in modified dendrimer-based delivery system in mouse model

2017 ◽  
Vol Volume 12 ◽  
pp. 8573-8585 ◽  
Author(s):  
Azadeh Bahadoran ◽  
Mehdi Ebrahimi ◽  
Swee Keong Yeap ◽  
Nikoo Safi ◽  
Hassan Moeini ◽  
...  
Keyword(s):  
Immune Response ◽  
Influenza Virus ◽  
Mouse Model ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Dna Vaccine ◽  
Delivery System ◽  
Robust Immune Response

scholarly journals DNA Vaccine Encoding Hemagglutinin Provides Protective Immunity against H5N1 Influenza Virus Infection in Mice

1999 ◽  
Vol 73 (3) ◽  
pp. 2094-2098 ◽  
Author(s):  
Shantha Kodihalli ◽  
Hideo Goto ◽  
Darwyn L. Kobasa ◽  
Scott Krauss ◽  
Yoshihiro Kawaoka ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Dna Vaccine ◽  
Influenza Virus Infection ◽  
Glycosylation Site ◽  
Mammalian Host ◽  
H5n1 Infection ◽  
H5n1 Avian Influenza

ABSTRACT In Hong Kong in 1997, a highly lethal H5N1 avian influenza virus was apparently transmitted directly from chickens to humans with no intermediate mammalian host and caused 18 confirmed infections and six deaths. Strategies must be developed to deal with this virus if it should reappear, and prospective vaccines must be developed to anticipate a future pandemic. We have determined that unadapted H5N1 viruses are pathogenic in mice, which provides a well-defined mammalian system for immunological studies of lethal avian influenza virus infection. We report that a DNA vaccine encoding hemagglutinin from the index human influenza isolate A/HK/156/97 provides immunity against H5N1 infection of mice. This immunity was induced against both the homologous A/HK/156/97 (H5N1) virus, which has no glycosylation site at residue 154, and chicken isolate A/Ck/HK/258/97 (H5N1), which does have a glycosylation site at residue 154. The mouse model system should allow rapid evaluation of the vaccine’s protective efficacy in a mammalian host. In our previous study using an avian model, DNA encoding hemagglutinin conferred protection against challenge with antigenic variants that differed from the primary antigen by 11 to 13% in the HA1 region. However, in our current study we found that a DNA vaccine encoding the hemagglutinin from A/Ty/Ir/1/83 (H5N8), which differs from A/HK/156/97 (H5N1) by 12% in HA1, prevented death but not H5N1 infection in mice. Therefore, a DNA vaccine made with a heterologous H5 strain did not prevent infection by H5N1 avian influenza viruses in mice but was useful in preventing death.

scholarly journals Intranasal administration of inactivated avian influenza virus of H5N1 subtype vaccine-induced systemic immune response in chicken and mice

2018 ◽  
Vol 11 (2) ◽  
pp. 221-226 ◽  
Author(s):  
I N. Suartha ◽  
G. A. A. Suartini ◽  
I W. Wirata ◽  
N. M. A. R. K. Dewi ◽  
G. N. N. Putra ◽  
...  
Keyword(s):  
Immune Response ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Intranasal Administration ◽  
Systemic Immune Response ◽  
H5n1 Subtype
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