scholarly journals Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

2009 ◽  
Vol 10 (4) ◽  
pp. 331 ◽  
Author(s):  
Seong In Lim ◽  
Wooseog Jeong ◽  
Dong Seob Tark ◽  
Dong Kun Yang ◽  
Chang Hee Kweon
Keyword(s):  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Envelope ◽  
Agar Gel ◽  
Immunodiffusion Analysis

Transient expression of a bovine leukemia virus envelope glycoprotein in plants by a recombinant TBSV vector

2018 ◽  
Vol 255 ◽  
pp. 1-7 ◽  
Author(s):  
Aiganym T. Zhumabek ◽  
Laura S. Abeuova ◽  
Nurzhan S. Mukhametzhanov ◽  
Herman B. Scholthof ◽  
Yerlan M. Ramankulov ◽  
...  
Keyword(s):  
Transient Expression ◽  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Envelope

Synthetic peptides approach to identification of epitopes on bovine leukemia virus envelope glycoprotein gp51

Virology ◽  
1989 ◽  
Vol 169 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Daniel Portetelle ◽  
Corine Dandoy ◽  
Arsène Burny ◽  
Jan Zavada ◽  
Helga Siakkou ◽  
...  
Keyword(s):  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Synthetic Peptides ◽  
Leukemia Virus ◽  
Virus Envelope

Comparative processing of bovine leukemia virus envelope glycoprotein gp72 by subtilisin/kexin-like mammalian convertases

FEBS Letters ◽  
1997 ◽  
Vol 406 (1-2) ◽  
pp. 205-210 ◽  
Author(s):  
Sarra Zarkik ◽  
Etienne Decroly ◽  
Ruddy Wattiez ◽  
Nabil G Seidah ◽  
Arsène Burny ◽  
...  
Keyword(s):  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Envelope ◽  
Comparative Processing

scholarly journals Expression of the Bovine Leukemia Virus Envelope Glycoprotein (gp51) by Recombinant Baculovirus and Its Use in an Enzyme-Linked Immunosorbent Assay

2004 ◽  
Vol 11 (1) ◽  
pp. 147-151 ◽  
Author(s):  
Antonio De Giuseppe ◽  
Francesco Feliziani ◽  
Domenico Rutili ◽  
Gian Mario De Mia
Keyword(s):  
Immunosorbent Assay ◽  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Signal Sequence ◽  
Leukemia Virus ◽  
Recombinant Baculovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Envelope ◽  
Gene Encoding ◽  
Field Samples

ABSTRACT The gene encoding the major envelope glycoprotein (gp51) with its signal sequence, represented by an additional NH2-terminal 33-residue amino acid sequence of bovine leukemia virus (BLV), was inserted into a baculovirus transfer vector. A recombinant virus expressing a secreted gp51 protein in insect cells was isolated. The recombinant gp51 expressed was characterized by using an anti-BLV monoclonal antibody by both Western blotting analysis and enzyme-linked immunosorbent assay (ELISA). The secreted gp51 was used as an antigen, and an ELISA with recombinant gp51 (rgp51) was developed for the detection of BLV antibodies. This new procedure was compared with a previous ELISA method for the detection of BLV antibodies and an agar gel immunodiffusion test performed with an unpurified BLV antigen preparation. The comparative testing of field samples showed that the ELISA with rgp51 is more specific and also suitable for the testing of pooled sera.

Antigenic variants of bovine leukemia virus (BLV) are defined by amino acid substitutions in the NH2 part of the envelope glycoprotein gp51

Virology ◽  
1989 ◽  
Vol 169 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Daniel Portetelle ◽  
Dominique Couez ◽  
Claudine Bruck ◽  
Richard Kettmann ◽  
Marc Mammerickx ◽  
...  
Keyword(s):  
Amino Acid ◽  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Amino Acid Substitutions

Lack of association between amino acid sequences of the bovine leukemia virus envelope and varying stages of infection in dairy cattle

2020 ◽  
Vol 278 ◽  
pp. 197866
Author(s):  
Fernando Cerón Téllez ◽  
Ana Silvia González Méndez ◽  
Jorge Luis Tórtora Pérez ◽  
Elizabeth Loza-Rubio ◽  
Hugo Ramírez Álvarez
Keyword(s):  
Amino Acid ◽  
Dairy Cattle ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Amino Acid Sequences ◽  
Virus Envelope
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