Isolation of protoplasts from viable egg cells of common buckwheat (Fagopyrum esculentum Moench.)

1999 ◽  
Vol 79 (4) ◽  
pp. 593-595 ◽  
Author(s):  
Sun Hee Woo ◽  
Taiji Adachi ◽  
Seung Keun Jong ◽  
Clayton G. Campbell
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Sac ◽  
Fagopyrum Esculentum ◽  
Enzyme Treatment ◽  
Egg Cell ◽  
Common Buckwheat ◽  
Vitro Fertilization ◽  
Mechanical Isolation ◽  
Fagopyrum Esculentum Moench

In vitro fertilization could be useful to overcome the problem of self-incompatibility of common buckwheat. Therefore, experiments were conducted to isolate viable protoplasts from egg cells for use in vitro fertilization. Protoplasts from viable egg cells of common buckwheat were isolated by enzyme treatment followed by mechanical isolation. Incubation of ovules for up to 4 h allowed the boundary wall of the egg cells to become partially digested and the protoplasts of the egg cells came out of the micropylar or chalazal end after squashing with a mean isolation frequency of approximately 30% (6 out of 20 ovules). The egg cell protoplasts were larger than those of the synergids. Key words: Common buckwheat, Fagopyrum esculentum Moench., protoplast, egg cell solation, embryo sac

scholarly journals In vitro Cross Breeding in Plant-intraspecific Hybrid Production of Common Buckwheat (Fagopyrum esculentum Moench.) Plant in vitro

2007 ◽  
Vol 19 (2) ◽  
pp. 89-95
Author(s):  
Wanna MANGKITA ◽  
Prapapan PIMKAEW ◽  
Yongsak KACHONPADUNGKITTI ◽  
Ryo OHSAWA ◽  
Shigeru HISAJIMA
Keyword(s):  
Fagopyrum Esculentum ◽  
Common Buckwheat ◽  
Cross Breeding ◽  
Hybrid Production ◽  
Fagopyrum Esculentum Moench ◽  
Intraspecific Hybrid

Embryological background of low seed set in distylous common buckwheat (Fagopyrum esculentum Moench) with biased morph ratios, and biostimulant-induced improvement of it

2017 ◽  
Vol 68 (7) ◽  
pp. 680 ◽  
Author(s):  
Aneta Słomka ◽  
Klaudia Michno ◽  
Franciszek Dubert ◽  
Michał Dziurka ◽  
Przemysław Kopeć ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Seed Set ◽  
Embryo Sac ◽  
Mature Embryo ◽  
Fagopyrum Esculentum ◽  
Naphthaleneacetic Acid ◽  
Common Buckwheat ◽  
Morph Ratios ◽  
Fagopyrum Esculentum Moench ◽  
Compatible Pollen

The biased ratio (1 : 2.7–1 : 19) of long-styled Pin and short-styled Thrum flowers (anisoplethy) in common buckwheat (Fagopyrum esculentum) with low seed set (9.8–33.1%) is documented for the first time in two cultivars (Kora, Panda) and two strains (PA13, PA14). To establish the reasons for low grain yield we studied pollen, embryo sacs, embryos, counted stigmas with compatible pollen and with compatible pollen tubes, and recorded seed set under semi-controlled conditions with open access of pollinators. We also sought to improve seed yield via exogenous application of eight biostimulants at the beginning of flowering. Pin pollen supply to Thrum stigmas was low, due to the imbalance of flower morphs. This did not affect seed set or male success in either flower morph. The pollen of Pin or Thrum was highly viable (97.9–99.9%) in all studied cultivars and strains, germinating well on compatible stigmas. The female success of both flower types was much lower; 49–59% of the ovules exhibited signs of degeneration (whole flower buds, ovules only) or abortion (mature embryo sacs, proembryos, embryos); the highest share of mature embryo sac abortions resulted from degeneration of synergids or the whole egg apparatus. Three biostimulants (Gibberellic acid, putrescine, Asahi SL) in PA13 and six (1-Naphthaleneacetic acid, Gibberellic acid, TYTANIT, putrescine, 6-Benzylaminopurine, Asahi SL) in PA14 decreased embryo abortions (4–12 fold) and increased seed set (0.4–2.4 times), but seed set was still low and never exceeded 33% (the highest value of the untreated with biostimulants plants). Biostimulant treatments were most effective on PA14 strain increasing seed set in 7 out of 8 treatments. These were Gibberellic acid, putrescine and Asahi SL improving seed set of two among four analysed genotypes.

Predictive Model for Live Birth at 12 Months After Starting In-Vitro Fertilization Treatment

MedPharmRes ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 5-20
Author(s):  
Vu Ho ◽  
Toan Pham ◽  
Tuong Ho ◽  
Lan Vuong
Keyword(s):  
In Vitro Fertilization ◽  
Live Birth ◽  
Cox Regression ◽  
Financial Burden ◽  
Oocyte Retrieval ◽  
Operating Characteristics ◽  
Vitro Fertilization ◽  
Cox Regression Analysis ◽  
Significant Difference

IVF carries a considerable physical, emotional and financial burden. Therefore, it would be useful to be able to predict the likelihood of success for each couple. The aim of this retrospective cohort study was to develop a prediction model to estimate the probability of a live birth at 12 months after one completed IVF cycle (all fresh and frozen embryo transfers from the same oocyte retrieval). We analyzed data collected from 2600 women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) at a single center in Vietnam between April 2014 and December 2015. All patients received gonadotropin-releasing hormone (GnRH) antagonist stimulation, followed by fresh and/or frozen embryo transfer (FET) on Day 3. Using Cox regression analysis, five predictive factors were identified: female age, total dose of recombinant follicle stimulating hormone used, type of trigger, fresh or FET during the first transfer, and number of subsequent FET after the first transfer. The area under the receiver operating characteristics curve for the final model was 0.63 (95% confidence interval [CI] 0.60‒0.65) and 0.60 (95% CI 0.57‒0.63) for the validation cohort. There was no significant difference between the predicted and observed probabilities of live birth (Hosmer-Lemeshow test, p > 0.05). The model developed had similar discrimination to existing models and could be implemented in clinical practice.

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