Micromanipulation of male and female gametes ofNicotiana tabacum: II. Preliminary attempts for in vitro fertilization and egg cell culture

1997 ◽  
Vol 16 (9) ◽  
pp. 657-661 ◽  
Author(s):  
H. Q. Tian ◽  
S. D. Russell
Keyword(s):  
Cell Culture ◽  
In Vitro Fertilization ◽  
Egg Cell ◽  
Male And Female ◽  
Vitro Fertilization ◽  
Ofnicotiana Tabacum

Transmeiotic differentiation of zebrafish germ cells into functional sperm in culture

Development ◽  
2002 ◽  
Vol 129 (14) ◽  
pp. 3359-3365 ◽  
Author(s):  
Noriyoshi Sakai
Keyword(s):  
Cell Culture ◽  
In Vitro Fertilization ◽  
Germ Cell ◽  
Germ Cells ◽  
Regulatory Function ◽  
Feeder Cells ◽  
Male Germ Cells ◽  
Vitro Fertilization ◽  
Testicular Cells

Because cell culture systems are easily accessible for experimental genetic manipulation, male germ cell culture is of great usefulness in creating sperm vectors. This report describes that cultured male germ cells of zebrafish (Danio rerio) underwent mitosis and transmeiotic differentiation, including the entire process of meiosis, to develop into functional sperm. Enzymatically dissociated testicular cells containing germ cells were co-cultured on feeder cells derived from tumor-like testis, which exhibited features characteristic of Sertoli cells such as phagocytic activity and transcription of the Wilms’ tumor suppressor wt1 and sox9a genes. Germ cells formed a clump, divided by mitosis, and differentiated into flagellated sperm on the feeders. Expression of the germ cell marker gene vas was prolonged in co-culture with the feeders, compared with culture of dissociated testicular cells alone, indicating that the feeder cells stimulate proliferation of spermatogonia. When cultured germ cells/sperm with the feeders were used for in vitro fertilization, normal embryos were obtained. Addition of the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) into culture medium resulted in BrdU-positive sperm and four-cell stage embryos after in vitro fertilization. This culture system should prove useful not only in producing transfected functional sperm, but also in analyzing the regulatory function of testicular somatic cells on the mitosis and meiosis of male germ cells in vertebrates.

Isolation of protoplasts from viable egg cells of common buckwheat (Fagopyrum esculentum Moench.)

1999 ◽  
Vol 79 (4) ◽  
pp. 593-595 ◽  
Author(s):  
Sun Hee Woo ◽  
Taiji Adachi ◽  
Seung Keun Jong ◽  
Clayton G. Campbell
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Sac ◽  
Fagopyrum Esculentum ◽  
Enzyme Treatment ◽  
Egg Cell ◽  
Common Buckwheat ◽  
Vitro Fertilization ◽  
Mechanical Isolation ◽  
Fagopyrum Esculentum Moench

In vitro fertilization could be useful to overcome the problem of self-incompatibility of common buckwheat. Therefore, experiments were conducted to isolate viable protoplasts from egg cells for use in vitro fertilization. Protoplasts from viable egg cells of common buckwheat were isolated by enzyme treatment followed by mechanical isolation. Incubation of ovules for up to 4 h allowed the boundary wall of the egg cells to become partially digested and the protoplasts of the egg cells came out of the micropylar or chalazal end after squashing with a mean isolation frequency of approximately 30% (6 out of 20 ovules). The egg cell protoplasts were larger than those of the synergids. Key words: Common buckwheat, Fagopyrum esculentum Moench., protoplast, egg cell solation, embryo sac

scholarly journals Effect of male and female body mass index on pregnancy and live birth success after in vitro fertilization

2015 ◽  
Vol 103 (2) ◽  
pp. 388-395 ◽  
Author(s):  
Karen C. Schliep ◽  
Sunni L. Mumford ◽  
Katherine A. Ahrens ◽  
James M. Hotaling ◽  
Douglas T. Carrell ◽  
...  
Keyword(s):  
Body Mass Index ◽  
In Vitro Fertilization ◽  
Body Mass ◽  
Live Birth ◽  
Female Body ◽  
Male And Female ◽  
Vitro Fertilization ◽  
Female Body Mass
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