Effect of NaCl on growth, morphology, and camptothecin accumulation in Camptotheca acuminata seedlings

2003 ◽  
Vol 83 (4) ◽  
pp. 931-938 ◽  
Author(s):  
Zhanhai Li and ◽  
Zhijun Liu
Keyword(s):  
Negative Relationship ◽  
Dry Weight ◽  
Leaf Length ◽  
Camptotheca Acuminata ◽  
Growth Media ◽  
Growth Morphology ◽  
Growth Environment ◽  
Plant Cultivation ◽  
Total Leaf ◽  
Camptotheca Acuminata Seedlings

NaCl salt is shown to increase alkaloid accumulation in some plant species, but creates an extensive problem in plant cultivation. The effect of NaCl on Camptotheca acuminata Decaisne growth, morphology, and camptothecin accumulation (concentration and yield) was studied in a hydroponic culture system. High NaCl levels (above 75 mM) in growth media significantly reduced plant height, weight, leaf number, and leaf length but increased leaf weight. In contrast, low NaCl levels (below 75 mM) only moderately reduced growth and affected morphology. Our results suggest a concentration of 75 mM is the threshold for NaCl to exert a detrimental effect on C. acuminata seedlings. Furthermore, high NaCl levels (above 75 mM) increased camptothecin concentrations both in leaves and roots, whereas low NaCl levels (below 75 mM) had no effect on camptothecin concentrations. However, total leaf camptothecin yield, the product of leaf camptothecin concentration and total leaf dry weight per seedling, decreased with increasing NaCl concentration. There was a negative relationship between plant growth and camptothecin concentration. Our results suggest that high NaCl salinity increases camptothecin concentration, but is not helpful to achieve a high camptothecin yield. Therefore, a salty growth environment should be avoided for camptothecin production. Key words: Alkaloid, anti-cancer plant, HPLC, plant secondary metabolism

Camptothecin production in Camptotheca acuminata cultured hydroponically and with nitrogen enrichments

2005 ◽  
Vol 85 (2) ◽  
pp. 447-452 ◽  
Author(s):  
Zhanhai Li ◽  
Zhijun Liu
Keyword(s):  
Culture Medium ◽  
Dry Weight ◽  
Leaf Length ◽  
Hydroponic Culture ◽  
Camptotheca Acuminata ◽  
Root Weight ◽  
Plant Weight ◽  
N Concentration ◽  
Total Leaf ◽  
High Concentrations

Camptothecin (CPT), produced by Camptotheca acuminata Decaisne (Nyssaceae), is becoming a therapeutically important compound, and the demand for CPT is increasing dramatically. Feasibility of CPT production from C. acuminata in hydroponic culture was studied. As nitrogen (N) is required for the biosynthesis of the alkaloid CPT, CPT production in C. acuminata was studied in response to enrichments of 7.5, 15.0, 22.5, or 30.0 mM N in the culture medium. High concentrations of CPT were found in leaves of plants in hydroponic culture, but the N concentration in the culture medium did not affect tissue CPT concentration, plant weight, plant height, leaf number, lamina dry weight per area, total stem weight, total leaf weight, or total leaf CPT yield (the product of leaf CPT concentration and total leaf dry weight per plant). However, increasing the N concentration in the culture medium reduced leaf length and total root weight. We conclude that C. acuminata CPT production in hydroponic culture is feasible in terms of efficiency for CPT production, and is a potential alternative to culture in the field or in pots, or cell or callus culture, and that a standard low N rate in the culture medium is recommended. Key words: Anti-cancer compound, HPLC, medicinal plant

scholarly journals Thermo-Chemical Treatment for Carcass Disposal and the Application of Treated Carcass as Compost

2021 ◽  
Vol 11 (1) ◽  
pp. 431
Author(s):  
Jae-In Lee ◽  
Eun-Ji Cho ◽  
Fritz Ndumbe Lyonga ◽  
Chang-Hee Lee ◽  
Sue-Yun Hwang ◽  
...  
Keyword(s):  
Chemical Treatment ◽  
Dry Weight ◽  
Heavy Metal Concentration ◽  
Leaf Length ◽  
Air Pollutant ◽  
Germination Index ◽  
Pollutant Emission ◽  
Reinforced Plastic ◽  
Korean Ministry ◽  
Index Value

A mechanized thermo-chemical treatment system was developed to treat the undecomposed carcass and remediate livestock burial sites. Animal carcasses were thus processed via crushing, mixing, and treatment with quicklime treatment, heat treatment (200–500 °C), and mixing with sawdust. The machinery was applied to two sites where 16,000 chickens and 418 pigs had previously been buried in fiber-reinforced plastic storage bins. No dioxins were detected in the gas discharged during processing, and the concentration of total volatile organic compound, toluene, ethylbenzene, xylene, and styrene were 430.3, 139.0, 18.3, 21.4, and 10.4 μg/m3, respectively, which were below the air pollutant emission standards issued by the Korean Ministry of Environment. Korean standards stipulating the use of treated carcasses as compost (C, N, and P content, heavy metal concentration, Escherichia coli, and Salmonella) were met, but the germination index value was less than 70, not satisfying the criteria. Plant height, leaf length, leaf width, and dry weight of lettuce grown in soil amended with treated carcass product were significantly lower than those grown in low nutrient soil due to the poor germination index of the treated carcass. These results indicate that a composting process is required before the use of the treated carcass as a fertilizer. The addition of zeolite retarded the elution of ammonia from the carcasses and its efficiency was about 87.9%. It is expected that the mechanized thermo-chemical treatment process developed in this study could replace other technologies for the remediation of livestock burial sites.

scholarly journals The Influence of Biochar and Solid Digestate on Rose-Scented Geranium (Pelargonium graveolens L’Hér.) Productivity and Essential Oil Quality

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 260 ◽  
Author(s):  
Alessandro Calamai ◽  
Enrico Palchetti ◽  
Alberto Masoni ◽  
Lorenzo Marini ◽  
David Chiaramonti ◽  
...  
Keyword(s):  
Essential Oil ◽  
Climate Adaptation ◽  
Oil Quality ◽  
Dry Weight ◽  
Chemical Components ◽  
Growth Media ◽  
Anaerobic Digestate ◽  
Pelargonium Graveolens ◽  
Positive Effects ◽  
Leaf Chlorosis

In recent years, biochar has generated global interest in the areas of sustainable agriculture and climate adaptation. The main positive effects of biochar were observed to be the most remarkable when nutrient-rich feedstock was used as the initial pyrolysis material (i.e., anaerobic digestate). In this study, the influence of solid anaerobic digestate and biochar that was produced by the slow pyrolysis of solid digestate was evaluated by comparing the differences in the crop growth performances of Pelargonium graveolens. The experiment was conducted in a greenhouse while using three different growth media (i.e., solid digestate, biochar, and vermiculite). The results indicated that: (i) the pyrolysis of solid digestate caused a reduction in the bulk density (−52%) and an increase in the pH (+16%) and electrical conductivity (+9.5%) in the derived biochar; (ii) the best crop performances (number of leaves, number of total branches, and plant dry weight) were found using biochar, particularly for plant dry weight (+11.4%) and essential oil content (+9.4%); (iii) the essential oil quality was slightly affected by the growth media; however, the main chemical components were found within the acceptable range that was set by international standard trade; and, iv) biochar induced the presence of leaf chlorosis in Pelargonium graveolens.

scholarly journals Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  
Keyword(s):  
Drought Stress ◽  
Field Capacity ◽  
Dry Weight ◽  
Positive Control ◽  
Morphological Characterization ◽  
Leaf Length ◽  
Selenium Nanoparticles ◽  
Fresh Weight ◽  
Wheat Varieties ◽  
Shoot Dry Weight

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.

scholarly journals Growth and Yield Response of Rodent Tuber (Typhonium flagelliforme (Lodd.) Blume) under Different Light Intensities and Concentrations of Paclobutrazol

2016 ◽  
Vol 1 (3) ◽  
pp. 29-35
Author(s):  
Noorwitri Utami ◽  
Lukita Devy ◽  
Arief Arianto
Keyword(s):  
Light Intensity ◽  
Concentration Level ◽  
Dry Weight ◽  
Leaf Length ◽  
Growth And Yield ◽  
Yield Response ◽  
Full Sunlight ◽  
Anticancer Treatment ◽  
Main Plot ◽  
Number Of Leaves

Rodent tuber (Typhonium flagelliforme (Lodd) Blume) is one of the medicinal plants used for anticancer treatment, but the information on the cultivation of the plant is limited. The objectives of this research was to study the effect of light intensity and concentration level of paclobutrazol on growth and yield of rodent tuber. This research was conducted at Puspiptek Serpong, Tangerang Selatan, Indonesia. This experiment arranged in a split plot design with three replications. The main plot is light intensity (35, 55, and 100%). The sub plot is concentration level of paclobutrazol (0, 50, 100, and 150 ppm). Paclobutrazol applied as soil drench at one month after planting. In each application 500 ml solution was used. Variables observed consisted of plant height, number of leaves, leaf length, leaf width, leaf and tuber fresh weight; and leaf and tuber dry weight. Data were analyzed using analysis of variance and Duncan Multiple Range test at 5%. The result showed that interaction between light intensity and paclobutrazol affected the whole observed variable. Dry weight of tuber under 35% light intensity was lower than those in other light intensity for all treatments of paclobutrazol. Full sunlight intensity showed better dry weight in all treatments of paclobutrazol. However the dry weight decreasing significantly at 150 ppm paclobutrazol, but still higher than those in 35% light intensity. Therefore, rodent tuber is better cultivated under full sunlight and 55% light intensity with 100 ppm paclobutrazol.

scholarly journals IDENTIFICATION OF LEAD AND CADMIUM LEVELS IN WHITE CABBAGE (Brassica rapa L.), SOIL, AND IRRIGATION WATER OF URBAN AGRICULTURAL SITES IN THE PHILIPPINES

2016 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Hardiyanto Hardiyanto ◽  
Constancio C. De Guzman
Keyword(s):  
Brassica Rapa ◽  
Urban Agriculture ◽  
Irrigation Water ◽  
Negative Relationship ◽  
Dry Weight ◽  
The Philippines ◽  
Main Road ◽  
Lead And Cadmium ◽  
White Cabbage ◽  
Significant Difference

Urban agriculture comprises a variety of farming systems, ranging from subsistence to fully commercialized agriculture. Pollution from automobile exhaust, industrial and commercial<br />activities may affect humans, crops, soil, and water in and around urban agriculture areas. The research aimed to investigate the level and distribution of lead (Pb) and cadmium (Cd) in white cabbage (Brassica rapa L.), soil, and irrigation water taken from urban sites. The research was conducted in Las Piñas and Parañaque, Metro Manila, Philippines. The field area was divided into three sections based on its distance from the main road (0, 25, and 50 m). Irrigation water was taken from canal (Las Piñas) and river (Parañaque). Pb and Cd contents of the extract were measured by Atomic Absorption Spectrophotometry. Combined analysis over locations was used. The relationship between distance from the main road and metal contents was measured by Pearson’s correlation. Based on combined analyses, highly significant difference over locations was only showed on Cd content in white cabbage. Cd content in white cabbage grown in Parañaque was higher than that cultivated in Las Piñas, while Cd content in the soil between both sites was comparable.<br />The average Pb content (1.09 µg g-1 dry weight) was highest in the white cabbage grown right beside the main road. A similar trend was also observed in the soil, with the highest concentration being recorded at 26 µg g-1 dry weight. There was a negative relationship between distance from the main road and Pb and Cd contents in white cabbage and the soil. Level of Pb in water taken from the canal and river was similar (0.12 mg l-1), whereas<br />levels of Cd were 0.0084 and 0.0095 mg l-1, respectively. In general, the concentrations of Pb and Cd in white cabbage and soil as well as irrigation water were still in the acceptable limits. In terms of environmental hazards and polluted city environment, it seems that big cities in Indonesia especially Jakarta and Surabaya have the same problem with the Philippines. Therefore, it is suggested that the study on heavy metal contamination<br />in several crops, especially those grown along the main road, should be conducted.

scholarly journals Uji Isolat Bakteri Azotobacter Asal Kebun Lidah Buaya dengan Pupuk Urea terhadap Serapan Nitrogen pada Tanaman Kedelai (Glycine Max L.) di Tanah Gambut

2021 ◽  
Vol 2 ◽  
pp. 131-138
Author(s):  
Nandung Erlanda ◽  
Feira B. Arief ◽  
Ismahan Umran ◽  
Sutarman Gafur ◽  
Denah Suswati
Keyword(s):  
Nitrogen Uptake ◽  
Peat Soil ◽  
Aloe Vera ◽  
Dry Weight ◽  
Growth Media ◽  
Chemical Fertilizers ◽  
Urea Fertilizer ◽  
Randomized Design ◽  
The Media ◽  
Soybean Plants

Azotobacter nitrogen-fixing bacteria and urea fertilizer to reduce excessive use of chemical fertilizers. These bacteria can play a role in increasing nitrogen uptake and further growth of soybean plants. This study aimed to obtain growth media for Azotobacter and increase plant and root growth and nitrogen uptake of Biosoy soybean varieties. The research was conducted to help the uptake of n in the soil and reduce the excessive use of chemical fertilizers. The first experiment was carried out by growing bacteria on the media for 120 hours. The second experiment was designed in a completely randomized design that tested two treatments with additional use of urea fertilizer and Azotobacter and no Azotobacter. The results showed that there was no effect of giving Azotobacter from aloe vera gardens, and urea fertilizer showed a significant effect on the observed variables of soil pH in soybean plants on peat soil. In contrast, the variables observed were population, plant height, N content, N-total, plant dry weight, dry weight of plant roots, and N span of soybean plants had no significant effect.

Camptothecin accumulation in Camptotheca acuminata seedlings in response to acetylsalicylic acid treatment

2004 ◽  
Vol 84 (3) ◽  
pp. 885-889 ◽  
Author(s):  
Zhanhai Li ◽  
Zhijun Liu
Keyword(s):  
Acetylsalicylic Acid ◽  
Key Words ◽  
Acid Treatment ◽  
Hydroponic Culture ◽  
Camptotheca Acuminata ◽  
Plant Secondary Metabolite ◽  
Stunted Growth ◽  
Anti Cancer ◽  
Camptotheca Acuminata Seedlings ◽  
Potential Tool

Camptothecin is an anti-cancer alkaloid. Acetylsalicylic acid (ASA) treatment was evaluated as a potential tool for increasing camptothecin accumulation (concentration and yield) in Camptotheca acuminata. Increasing ASA concentration increased leaf camptothecin concentration, but stunted growth and decreased total leaf camptothecin yield. Thus, ASA treatment does not enhance camptothecin yield. Key words: Hydroponic culture, plant secondary metabolite

scholarly journals Effect of green manure along with nitrogenous fertilizer on the growth and yield of turmeric (curcuma longa l.) of Bangladesh

2018 ◽  
Vol 6 (1) ◽  
pp. 140
Author(s):  
Mahbuba Ferdous ◽  
Mohammad Kabirul Islam ◽  
Md. Monirul Islam ◽  
Md Isfatuzzaman Bhuyan ◽  
Md Sazedul Islam ◽  
...  
Keyword(s):  
Green Manure ◽  
Curcuma Longa ◽  
Maximum Yield ◽  
Coastal Region ◽  
Dry Weight ◽  
Leaf Length ◽  
Growth And Yield ◽  
Fresh Weight ◽  
Nitrogenous Fertilizer ◽  
Combined Application

Turmeric is an important spice crop of Bangladesh. A field experiment was conducted at farmer’s field of North Jhatibunia, Mirzagonj, Patuakhali during April, 2015 to February, 2016. The study was performed to find out the effect of green manure and nitrogenous fertilizer on the growth and yield of turmeric (BARI Halud-2), in coastal region of Bangladesh. In this study different combinations of green manure along with nitrogenous fertilizer were used. There was eight treatment combinations (viz. 7 ton biomass/ha + 100 kg N/ha, 7 ton biomass/ha + 80 kg N/ha, 7 ton biomass/ha + 60 kg N/ha, 7 ton biomass/ha + 40 kg N/ha, 7 ton biomass/ha + 20 kg N/ha, 7 ton biomass/ha, 100 kg N/ha and control). The highest number of leaves per plant (11.67) were recorded by the combined application of 7 ton biomass/ha along with 100 kg N/ha. The highest plant height (80.70 cm) and the longest leaf length (47.33 cm) were recorded by the application of 100 kg N/ha. The highest number of tillers per plant (7.67), maximum number of primary rhizomes per plant (9.00), maximum fresh weight of clump (189.67 g), highest fresh weight of roots (48.01 g), maximum fresh weight of rhizomes (277.70 g), maximum dry weight of clump (20.01 g), highest dry weight of roots (9.25 g), maximum dry weight of rhizomes (67.50 g) and maximum yield of fresh rhizome per hectare (6.64 tons) were recorded by the combined application of 7 ton /ha biomass+ 40 kg N/ha. Highest number of secondary rhizomes per plant (17.00) and maximum number of tertiary rhizomes per plant (7.00) were recorded by the application of 7 ton biomass/ha + 60 kg N/ha. With a few exception, the best performance in terms of growth and yield of turmeric were found from the combination of 7 ton biomass /ha + 40 kg N/ha.  

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