Growth responses of excised radish cotyledons to meta-topolin

2002 ◽  
Vol 82 (1) ◽  
pp. 191-194 ◽  
Author(s):  
Narçin Palavan-Ünsal ◽  
Serap Çağ ◽  
Ergül Çetin

Meta-topolin (mT) has been established as an active aromatic cytokinin by Strnad et al. (1997). The present investigation assessed the effects of mT on radish cotyledon growth. The effect of mT on cotyledon growth, chlorophyll, N and soluble protein contents were measured. Peroxidase (POD) activity and polyamine (PA) contents were also determined. Treatment at 0.25, 0.5 and 1.0 mM mT concentrations resulted in 44, 50 and 57% increments in cotyledon growth, respectively, compared with control cotyledons. Increase in chlorophyll content per cotyledon was highest at low mT concentrations. Peroxidase activity was highest at 1.0 mM mT. Soluble protein content was reduced by the application of mT. Key words: Cotyledon growth, meta-topolin, polyamine, peroxidase, radish cotyledons

Author(s):  
V. Divyabharathi ◽  
V. Swaminathan ◽  
P. Paramaguru ◽  
K. Venkatesan ◽  
T. Anitha ◽  
...  

A trial was conducted to assess the physiological and biochemical changes in M. oleifera (L.) Millsp.cv. PKM 1 induced by heading back and pinching treatments. Chlorophyll content, nitrate reductase content, soluble protein content and total phenol content were assessed after the new shoot initiated from heading back and pruning treatments before the flower initiation. Height and stage at which the apical growth was arrested significantly influenced chlorophyll, nitrate reductase, protein and phenol content in leaves. Heading back at 70 cm combined with pinching 100 days after heading back greatly influenced the physiological and biochemical factors except for soluble protein as it was increased by heading back at 30 cm.


2014 ◽  
Vol 1051 ◽  
pp. 333-340 ◽  
Author(s):  
Chun Tao Hong ◽  
Hai Peng Guo ◽  
Jia Fang ◽  
Wei Ren ◽  
Teng Fei Wang ◽  
...  

Miscanthus sacchariflorus is a typical perennial giant grass of East Asia as a biofuel feedstock. However, the mechanism of its salt tolerance is not well understood. In this study, chlorophyll a, b and total chlorophyll content, soluble protein content, MDA, proline content, POD, SOD, and CAT of M. sacchariflorus under salt stress were investigated with five NaCl concentrations ranging from 0 to 180 mmol.l-1. The results showed that the chlorophyll content decreased with increasing NaCl stresses, whereas soluble protein content, MDA, proline content, POD, SOD and CAT increased with increasing NaCl stresses. Low salt stress (30 and 60 mmol.l-1 NaCl) had almost no effect when comparing with the control under early stage (before 14 days) of salt stress treatment. However, treatment with high salt stress (90 and 180 mmol.l-1 NaCl) promoted MDA, proline, and antioxidant enzymes increasing markedly.


2011 ◽  
Vol 356-360 ◽  
pp. 263-266 ◽  
Author(s):  
Yuan Gao ◽  
Guan Ling Song ◽  
Hao Wu

After cultivating 4 days in Steinberg medium with different concentrations of Cd2+, the toxic effect of cadmiun on Azolla imbricata (Roxb.) Nakai was investigated for the purpose to find out if this plant is suitable to using on restoration of waterbody with cadmium pollution. The variations of chlorophyll content, soluble-protein content, peroxidase (POD) activity, catalase(CAT) activity, and malondialdehyde (MDA) content of Azolla imbricata (Roxb.) Nakai were measured in this study. The results show that the chlorophyll content of Azolla imbricata(Roxb.)Nakai has no significant changes in media with Cd2+concentrations compare with control. The MDA content ascended significantly from the Cd2+concentration was 5 mg•L-1, because of the intensified oxidative damage. When Cd2+concentration is 0.1~20 mg•L-1, the souble protein content, POD activity and CAT activity increased to resist the oxidative damage. Azolla imbricata (Roxb.) Nakai is tolerance of cadmium pollution, it can be used to water bodies restoration with low level cadmium pollution.


2021 ◽  
Author(s):  
Yiting Nan ◽  
Peiyong Guo ◽  
Hui Xing ◽  
Sijia Chen ◽  
Bo Hu ◽  
...  

Abstract The effects of different concentrations (100,150,200,250 mg/L) and different particle sizes (0–75µm, 75–120µm, 120–150µm, 150–500µm) on soluble protein content, SOD and CAT activity, MDA content, chlorophyll a content and photosynthetic parameters of Microcystis flos-aquae were studied, the mechanism of the effect of suspended particulate matter on the physiology and biochemistry of Microcystis flos-aquae was discussed. The results showed that the soluble protein content of Microcystis flos-aquae did not change obviously after being stressed by suspended particles of different concentration/diameter. The SOD activity of Microcystis flos-aquae increased at first and then decreased with the increase of the concentration of suspended particulate matter. The SOD activity of Microcystis flos-aquae reached 28.03 U/mL when the concentration of suspended particulate matter was 100 mg/L. The CAT activity of Microcystis flos-aquae increased with the increase of the concentration of suspended particles, and reached the maximum value of 12.45 U/mgprot in the concentration group of 250 mg/L, showing a certain dose-effect. The effect of small particle size on SOD, CAT and MDA of Microcystis flos-aquae was more significant than that of large particle size. The larger the concentration and the smaller the particle size, the stronger the attenuation of light and the lower the content of chlorophyll a. Both Fv/Fm and Fv/F0 of Microcystis flos-aquae increased at first and then decreased under different concentration/size of suspended particles. The relative electron transfer rate gradually returned to the normal level with the passage of time. There was no significant difference in α value between treatment group and control group, ETRmax and Ik decreased.


1970 ◽  
Vol 19 (2) ◽  
pp. 151-155
Author(s):  
Apurba Lal Ray ◽  
Mahbuba Akhter Jahan ◽  
Tahsina Rahim

Leaf extract of Citrus aurantifolia exhibited remarkable inhibitor effect on the radial mycelial growth of Neurospora crassa. The extract also showed mutagenic effect and atleast six morphological mutants of the fungus were detected including albino (al 243), vigorous (vg 117), fluffy (fl 220), colonial (cl 232), conidial band (con. band 171) and dirty (dir 83). The mutants were used for estimation of soluble protein in comparison with the wild type (Ema). The soluble protein content increased to some extent in case of the mutants con. band 171 (192.86 μg/ml), cl 232 (188.57 μg/ml) and vg 117 (186.43 μg/ml) as compared to the wild type (182.14 μg/ml). On the other hand, the soluble protein content was remarkably decreased in case of the mutant al 243 (94.28 μg/ml), which was about 50% less than the control. This indicates that the leaf extract not only effect colony morphology but possesses profound effect on growth and metabolism of the fungus. Key words: Neurospora crassa; Mutation; Leaf extract; Soluble protein DOI: http://dx.doi.org/10.3329/dujbs.v19i2.8958 DUJBS 2010; 19(2): 151-155


2011 ◽  
Vol 57 (No. 10) ◽  
pp. 459-464 ◽  
Author(s):  
Q.S. Wu

Citrus plants often suffer from temperature stress, which seriously inhibits tree growth and even results in tree death. The present experiment was conducted to evaluate the effects of Glomus mosseae on growth, root morphology, superoxide dismutase (SOD) and catalase (CAT) activities, and soluble protein content of trifoliate orange (Poncirus trifoliata) seedlings at low (15°C), optimum (25°C) and high (35°C) temperatures. Sixty-eight days after temperature stresses, mycorrhizal colonization and number of both entry points and vesicles were significantly inhibited by low or high temperature. Mycorrhizal seedlings recorded significantly higher growth characteristics than non-mycorrhizal seedlings at both optimum and high temperatures, but the beneficial effects were almost lost at low temperature. Generally, mycorrhizal seedlings presented notably higher root traits (projected area, surface area, number of forks and volume) than non-mycorrhizal seedlings regardless of temperature levels. Mycorrhizal colonization significantly increased SOD and CAT activities and soluble protein content at high temperature, increased only SOD activity at optimum temperature, and decreased only soluble protein content at low temperature. It suggests that mycorrhizal alleviation of temperature stress in trifoliate orange seedlings was at high temperature, but the alleviation was obviously weakened at low temperature.


Aerobiologia ◽  
2013 ◽  
Vol 29 (3) ◽  
pp. 443-447 ◽  
Author(s):  
H. Ribeiro ◽  
L. Duque ◽  
R. Sousa ◽  
I. Abreu

2010 ◽  
Vol 125 (3) ◽  
pp. 451-455 ◽  
Author(s):  
Edison Paulo Chu ◽  
Armando Reis Tavares ◽  
Shoey Kanashiro ◽  
Patricia Giampaoli ◽  
Erika Szeibel Yokota

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