Influence of temperature on interaction of resistance genes in spring wheat differentials with races of common bunt (Tilletia tritici and T. laevis)

1995 ◽  
Vol 75 (3) ◽  
pp. 745-749 ◽  
Author(s):  
D. A. Gaudet ◽  
B. J. Puchalski
Keyword(s):  
Spring Wheat ◽  
Resistance Genes ◽  
Specific Resistance ◽  
Common Bunt ◽  
Tilletia Tritici ◽  
Incubation Periods ◽  
Bt Genes ◽  
Different Temperatures ◽  
Influence Of Temperature On ◽  
Wheat Lines

Temperature affects the expression of resistance to common bunt and can influence the efficacy of tests to screen for resistance to this disease. A study was undertaken to determine the response of spring wheat differentials possessing bunt resistance genes to temperature and races of Tilletia tritici and T. laevis. Differential wheat lines carrying resistance genes Bt-2, Bt-7, Bt-8, Bt-9, and Bt-10, were individually inoculated with bunt races L3, T1, T15, and T27, and incubated at 8, 10 or 15 °C for 0–10 wk. Responses to bunt infection following low temperature incubation at 8, 10, and 15 °C differed among differentials carrying the Bt genes. The resistance of the Bt-10 differential was effective at all three temperatures for all incubation periods whereas the resistance in differentials carrying the Bt-2 or Bt-8 gene was effective at 15 °C but not at 8 °C and 10 °C. Resistance in the Bt-9 differential was ineffective at all three temperatures. The four races of common bunt were similar in their level of infection in the differentials carrying individual Bt genes at the different temperatures although some differences in infection levels were observed. A screening test consisting of 2–4 wk growth at 15 °C followed by growth to maturity in the greenhouse can identify wheat lines containing Bt-8 and Bt-10. Key words:Triticum aestivum, smut, race specific resistance

Culm height and susceptibility of Canadian winter and spring wheat cultivars to common bunt (Tilletia tritici and T. laevis)

1991 ◽  
Vol 71 (3) ◽  
pp. 677-687 ◽  
Author(s):  
D. A. Gaudet ◽  
B. J. Puchalski ◽  
T. Entz
Keyword(s):  
Key Words ◽  
Spring Wheat ◽  
Specific Resistance ◽  
Wheat Cultivars ◽  
Common Bunt ◽  
Nonspecific Resistance ◽  
Tilletia Tritici ◽  
Cultivar Variation ◽  
Low Levels

A field study involving winter and spring wheat cultivars possessing race-specific and nonspecific resistance to Tilletia tritici and T. laevis was conducted to determine the effect of bunt on culm dwarfing, and the role of culm height in the susceptibility of wheat cultivars. Bunt-induced stunting, ranging from 2.4 to 21%, was observed in 20 of 25 cultivars and the severity was correlated with level of bunt (r = 0.86). Also, there was preferential infection of shorter culms by the bunt fungus and this appeared to be a major factor in the susceptibility of most cultivars in this study. Cultivars possessing moderate to high levels of nonspecific resistance such as Katepwa and Chinook had fewer short culms; these short culms were susceptible. A large proportion of the culms of short-statured or semi-dwarf cultivars such as HY320, Tobari 66, and the soft white spring wheats Fielder and Springfield, fell into the shortest height classes; uniformly these were highly susceptible to bunt infection. Laura and Roblin, cultivars of conventional height, had a large proportion of short culms and these were highly susceptible. Red Bobs 222 and HY355 also exhibited high levels of bunt in the taller culms. Cultivars possessing race-specific resistance such as the durum wheats and BW553 had low levels of infection across the culm height classes. Cultivar variation in susceptibility to bunt within each of the different culm height classes also was observed. The possible implications of the effect of preferential bunting of short culms on development in short-statured or semi-dwarf wheats are discussed. Key words: Stinking smut, Norin 10/Brevor

scholarly journals Compatible and Incompatible Interactions in Wheat Involving the Bt-10 Gene for Resistance to Tilletia tritici, the Common Bunt Pathogen

2007 ◽  
Vol 97 (11) ◽  
pp. 1397-1405 ◽  
Author(s):  
Denis A. Gaudet ◽  
Zhen-Xiang Lu ◽  
Frances Leggett ◽  
Bryan Puchalski ◽  
André Laroche
Keyword(s):  
Compatible Interaction ◽  
Incompatible Interaction ◽  
Common Bunt ◽  
Tilletia Tritici ◽  
Pathogen Invasion ◽  
Reaction Zones ◽  
The Common ◽  
Wheat Lines ◽  
Compatible Interactions ◽  
Incompatible Interactions

The infection of wheat lines Neepawa (susceptible), and its sib BW553 that is nearly isogenic for the Bt-10 resistance gene by differentially virulent races T1 and T27 of common bunt (Tilletia tritici), was followed for 21 days following seeding (dfs) using fluorescence and confocal microscopy. Spore germination was nonsynchronous and all spore stages including germination were observed 5 to 21 dfs. Initial host perception of pathogen invasion, based on autofluorescence in epidermal cells adjacent to the appressoria, was similar in both compatible and incompatible interactions, and occurred as early as 5 to 6 dfs. The total number of sites on a 1-cm segment of coleoptile adjacent to the seed that exhibited autofluorescence was similar in both the compatible and incompatible interactions and rose to a maximum of 35 to 40 per 1 cm length of coleoptile following 17 dfs, although new infection events were observed as late as 21 dfs. In the compatible interaction, the autofluorescence became more diffuse 10 to 12 dfs, emanating in all directions in association with fungal spread. In the incompatible interaction, autofluorescence remained restricted to a small area surrounding the penetration site. Two different reaction zones that extended further in tissues surrounding the penetration point in the incompatible interaction compared with the compatible interaction were identified. The accumulation of callose around invading fungal hyphae was observed during both the compatible and incompatible interactions from 8 to 21 dfs. While callose accumulation was more extensive and widespread in the incompatible interaction, it was clearly present in compatible interactions, particularly in treatments involving BW553. These results were confirmed by expression of callose synthase transcripts that were more abundant in BW553 than in Neepawa and were upregulated during pathogen infection in both compatible and incompatible interactions.

Resistance genes and sources for the control of wheat common bunt (Tilletia tritici (DC.) Tul.)

Biologija ◽  
2008 ◽  
Vol 54 (4) ◽  
pp. 274-278 ◽  
Author(s):  
Žilvinas Liatukas ◽  
Vytautas Ruzgas
Keyword(s):  
Resistance Genes ◽  
Common Bunt ◽  
Tilletia Tritici

Investigating conditions that induce late maturity alpha-amylase (LMA) using Northwestern US spring wheat (Triticum aestivum L.)

2021 ◽  
pp. 1-9 ◽  
Author(s):  
Chang Liu ◽  
Keiko M. Tuttle ◽  
Kimberly A. Garland Campbell ◽  
Michael O. Pumphrey ◽  
Camille M. Steber
Keyword(s):  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Preharvest Sprouting ◽  
Cool Temperature ◽  
Grain Development ◽  
Cold Temperatures ◽  
Different Temperatures ◽  
Soft White Spring Wheat ◽  
Late Maturity ◽  
Wheat Lines

Abstract The wheat industry rejects grain with unacceptably high α-amylase enzyme levels due to the risk of poor endproduct quality. There are two main causes of elevated grain α-amylase: (1) preharvest sprouting in response to rain before harvest and (2) late maturity α-amylase (LMA) induction in response to a cool temperature shock during late grain development. LMA induction was detected in a panel of 24 Northwestern US spring wheat lines. Thus, this problem previously described in Australian and U.K. varieties also exists in U.S. varieties. Because LMA induction results were highly variable using published methods, a characterization of LMA-inducing conditions was conducted in an LMA-susceptible soft white spring wheat line, WA8124. Problems with elevated α-amylase in untreated controls were reduced by raising the temperature, 25°C day/18°C night versus 20°C day/10°C night. LMA induction was not improved by colder temperatures (15°C day/4°C night) versus moderately cold temperatures (18°C day/7.5°C night or 10°C day/10°C night). While previous studies observed LMA induction by heat stress, it failed to induce LMA in WA8124. Thus, not all LMA-susceptible cultivars respond to heat. The timing of LMA susceptibility varied between two cultivars and within a single cultivar grown at slightly different temperatures. Thus, variability in LMA induction likely results from variability in the timing of the grain developmental stage during which cold shock induces LMA. Thus, it was concluded that the visual inspection of grain is needed to correctly identify LMA-sensitive spikes at the soft dough stage of grain development (Zadok's stage 85).

Susceptibility and resistance in Canadian spring wheat cultivars to common bunt (Tilletia tritici and T. laevis)

1993 ◽  
Vol 73 (4) ◽  
pp. 1217-1224 ◽  
Author(s):  
D. A. Gaudet ◽  
B. J. Puchalski ◽  
G. C. Kozub ◽  
G. B. Schaalje
Keyword(s):  
Triticum Aestivum ◽  
Stability Analysis ◽  
Spring Wheat ◽  
Environment Interaction ◽  
Wheat Cultivars ◽  
Common Bunt ◽  
Hard Red Spring Wheat ◽  
Tilletia Tritici ◽  
High Infection ◽  
Susceptibility And Resistance

Current western Canadian hard red spring wheat cultivars and their progenitors, inoculated with a composite of Tilletia tritici and T. laevis races, were grown at Lethbridge from 1989 to 1991 and the percentage infection was determined. This study also examined cultivar × environment interaction effects. Ancestral cultivars, such as Red Calcutta 33, Marquis, Thatcher, and their descendants, Canthatch, Park, Neepawa, Manitou, and Cadet, exhibited intermediate levels of bunt resistance. Cultivars including the cultivar Hope in their ancestry, such as Redman, Chris, Katepwa, Columbus, Reward, and Lancer, were highly resistant. Analysis of the environment × cultivar interaction indicated that bunt infection levels in Hope, Redman, and Columbus were stable over environments, but infection which varied according to environment was observed for Katepwa and Reward. The reduction of a number of cultivars including Neepawa, Thatcher, Kenya Farmer, Fortuna, Cadet, and Chris, was unstable across environment. Mexican semi-dwarf cultivars, such as Tobari 66, Potem 70, Cajeme 71, Ciano 67, Sonora 64, Inia 66, and the Canadian cultivars HY320, BW 90, and Laura, were highly susceptible. In general, the level of infection in these cultivars was uniform across environments. The nature of resistance in Canadian cultivars, the high infection levels of some Canadian cultivars and their Mexican semi-dwarf ancestors, and the variation in stability of infection levels of some cultivars across environments are discussed. Key words: Triticum aestivum, semi-dwarf, stability analysis, genotype × environment interactions

scholarly journals Stem rust in Western Siberia – race composition and effective resistance genes

2020 ◽  
Vol 24 (2) ◽  
pp. 131-138 ◽  
Author(s):  
V. P. Shamanin ◽  
I. V. Pototskaya ◽  
S. S. Shepelev ◽  
V. E. Pozherukova ◽  
E. A. Salina ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Stem Rust ◽  
Western Siberia ◽  
Economic Damage ◽  
Spring Bread Wheat ◽  
Wheat Varieties ◽  
Wheat Lines ◽  
Sr Genes

Stem rust in recent years has acquired an epiphytotic character, causing significant economic damage  for wheat production in some parts of Western Siberia. On the basis of a race composition study of the stem rust  populations collected in 2016–2017 in Omsk region and Altai Krai, 13 pathotypes in Omsk population and 10 in  Altai population were identified. The race differentiation of stem rust using a tester set of 20 North American  Sr genes differentiator lines was carried out. The genes of stem rust pathotypes of the Omsk population are avirulent only to the resistance gene Sr31, Altai isolates are avirulent not only to Sr31, but also to Sr24, and Sr30. A low  frequency of virulence (10–25 %) of the Omsk population pathotypes was found for Sr11, Sr24,Sr30, and for Altai  population – Sr7b,Sr9b,Sr11,SrTmp, which are ineffective in Omsk region. Field evaluations of resistance to stem  rust were made in 2016–2018 in Omsk region in the varieties and spring wheat lines from three different sources.  The first set included 58 lines and spring bread wheat varieties with identified Sr genes – the so-called trap nursery  (ISRTN – International Stem Rust Trap Nursery). The second set included spring wheat lines from the Arsenal collection, that were previously selected according to a complex of economically valuable traits, with genes for resistance  to stem rust, including genes introgressed into the common wheat genome from wild cereal species. The third  set included spring bread wheat varieties created in the Omsk State Agrarian University within the framework of  a shuttle breeding program, with a synthetic wheat with the Ae. tauschiigenome in their pedigrees. It was established that the resistance genes Sr31, Sr40,Sr2 complexare effective against stem rust in the conditions of Western  Siberia. The following sources with effective Srgenes were selected: (Benno)/6*LMPG-6 DK42, Seri 82, Cham 10,  Bacanora (Sr31), RL 6087 Dyck (Sr40), Amigo (Sr24,1RS-Am), Siouxland (Sr24,Sr31), Roughrider (Sr6, Sr36), Sisson  (Sr6,Sr31,Sr36), and Fleming (Sr6,Sr24,Sr36,1RS-Am), Pavon 76 (Sr2 complex) from the ISRTN nursery; No. 1 BC 1F2 (96 × 113) × 145 × 113 (Sr2,Sr36,Sr44), No. 14а F 3(96 × 113) × 145 (Sr36,Sr44), No. 19 BC 2F3(96 × 113) × 113 (Sr2, Sr36, Sr44), and No. 20 F 3 (96 × 113) × 145  (Sr2,Sr36,Sr40, Sr44) from the Arsenal collection; and the Omsk State Agrarian  University varieties Element 22 (Sr31,Sr35), Lutescens 27-12, Lutescens 87-12 (Sr23,Sr36), Lutescens 70-13, and  Lutescens 87-13 (Sr23,Sr31,Sr36). These sources are recommended for inclusion in the breeding process for developing stem rust resistant varieties in the region.  

scholarly journals Reaction of spring wheat cultivars to common bunt caused by Tilletia tritici (Bjerk.) Wint. and Tilletia laevis (Kühn)

2008 ◽  
Vol 43 (No. 3) ◽  
pp. 82-86 ◽  
Author(s):  
V. Dumalasová ◽  
P. Bartoš
Keyword(s):  
Root System ◽  
Plant Height ◽  
Spring Wheat ◽  
Field Trials ◽  
Wheat Cultivars ◽  
Common Bunt ◽  
Tilletia Tritici ◽  
Greenhouse Experiments ◽  
Tilletia Laevis

In 2005, 2006 and 2007 nineteen, eight and nine spring wheat cultivars, respectively, were tested in field trials for resistance to common bunt after inoculation with bunt teliospores. Nine spring wheat cultivars were tested in a greenhouse under favourable conditions for the bunt infection. Bunt incidence in the field trials varied between 0% and 38.7%, in the greenhouse between 52.9% and 100%. The results of individual cultivars and years fluctuated. A reduction in plant height, ear length, root system and increased tillering were registered in the inoculated plants. Spots on the leaves of inoculated plants were observed in three out of the four greenhouse experiments.

STATUS OF BUNT RESISTANCE IN WESTERN CANADIAN SPRING WHEAT AND TRITICALE

1989 ◽  
Vol 69 (3) ◽  
pp. 797-804 ◽  
Author(s):  
D. A. GAUDET ◽  
B. J. PUCHALSKI
Keyword(s):  
Durum Wheat ◽  
Spring Wheat ◽  
Resistance Genes ◽  
Field Studies ◽  
Common Bunt ◽  
Canadian Prairie ◽  
Tilletia Caries ◽  
Hard Red Spring Wheat ◽  
Bt Resistance ◽  
Virulence Pattern

The reaction of western Canadian spring wheat and triticale to a composite of races of common bunt (Tilletia caries and T. foetida) was assessed in field studies conducted over 2 yr at three locations. Triticale and durum wheat were designated immune or highly resistant. Among cultivars of hard red spring wheat, Columbus was the most resistant followed by Katepwa, Leader, and Lancer. Neepawa, Park, and Marquis were intermediate in resistance. Roblin and Laura were susceptible. The Canadian Prairie Spring wheats HY320 and HY360 were highly susceptible and susceptible, respectively, whereas Oslo was intermediate in resistance. The utility wheats Wildcat and Glenlea were highly susceptible and intermediate, respectively. The soft white wheats Owens and Fielder were susceptible. The reaction of the cultivars to individual bunt races was evaluated under controlled environment conditions to identify specific Bt resistance genes. This revealed the presence of Bt1 in the cultivar Canuck and Bt10 in the line BW-553. Resistance in the other cultivars of hard red spring wheat appeared to be race nonspecific in nature. Race specificity was apparent in durum wheat but the virulence pattern could not be employed to identify specific Bt resistance genes. The potential impact of seeding large acreages to spring wheat cultivars that are highly susceptible to common bunt is discussed.Key words: Tilletia caries, Tilletia foetida, bunt (common)

scholarly journals Identification of New Pathogenic Races of Common Bunt and Dwarf Bunt Fungi, and Evaluation of Known Races Using an Expanded Set of Differential Wheat Lines

Plant Disease ◽  
2012 ◽  
Vol 96 (3) ◽  
pp. 361-369 ◽  
Author(s):  
Blair J. Goates
Keyword(s):  
Resistance Genes ◽  
Temperature Sensitive ◽  
Common Bunt ◽  
Tilletia Caries ◽  
Pathogenic Races ◽  
New Races ◽  
Wheat Lines ◽  
First Time ◽  
Dwarf Bunt ◽  
Bunt Resistance

Pathogenic races of Tilletia caries and T. foetida, which cause common bunt of wheat (Triticum aestivum), and Tilletia contraversa, which causes dwarf bunt of wheat, have been identified previously by their reaction to 10 differential wheat lines, each containing single bunt resistance genes Bt1 through Bt10. The reactions of races to the differential wheat lines follow the classic gene-for gene system for host–pathogen interactions. The pathogens are closely related and resistance to both diseases in wheat is controlled by the same genes. To better define pathogenic races, six additional wheat lines containing the genes Bt11 through Bt15 and a wheat line with a resistance factor designated as Btp were added to the set of 10 differentials and tested with all named U.S. races of common bunt and dwarf bunt. In addition, new isolates of dwarf bunt, and common bunt from hybrids and field collections, were tested with all 16 differentials for race identification. Six new races of T. caries, five new races of T. foetida, and two new races of T. contraversa were identified. Races of common bunt virulent to Bt8 or Bt12, and dwarf bunt races virulent to the combinations of Bt11 and Bt12, and Bt8, Bt9, Bt10, Bt11, and Bt12, were identified for the first time. Comparison of the reactions of the common bunt races with the Bt14 and Bt15 differentials grown in different environments after initial infection showed that these genes are temperature sensitive, indicating they should be excluded from the set of differential lines to avoid ambiguity in determining virulent or avirulent reactions. In the previous list of bunt races, there were races that had the same reaction to the set of 10 differentials but were designated as different races. These races were not differentiated further with the six additional differentials, indicating that the duplicate races should be dropped from the list of pathogenic races. The new races of common bunt and dwarf bunt identified have unique patterns of virulence that allow specific targeting and elucidation of bunt resistance genes in wheat and will aid the development of bunt-resistant wheat cultivars.

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