Identification de cultivars de framboisier par l’analyse d’ADN polymorphe amplifié au hasard (RAPD)

1993 ◽  
Vol 73 (4) ◽  
pp. 1115-1122 ◽  
Author(s):  
Jean-Guy Parent ◽  
Danièle Pagé ◽  
Marc G. Fortin
Keyword(s):  
Key Words ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Simple Test ◽  
Certification Program ◽  
Growth Season ◽  
Plant Dna ◽  
In Vitro Plantlets

We developed a fast and simple test to accurately characterize the various cultivars of raspberry of the Quebec certification program. This test, based on random amplified polymorphic DNA (RAPD) analysis, uses minute quantities of plant DNA. Portions of this DNA are multiplied with small 10-nucleotide primers of arbitrary sequences. Analyses were done on DNA from 15 raspberry cultivars amplified with 19 different primers. A combination of three primers was chosen for its ability to differenciate all the cultivars. Test reproducibility was checked with DNA samples from plants collected at various periods of the growth season and from plants of various categories of the certification program (in vitro plantlets, Foundation and Certified plants, commercial plants). Key words: Raspberry (Rubus spp.), random amplified polymorphic DNA, cultivar identification

Authentification des 13 cultivars de fraisier du programme de certification du Québec par l'analyse d'ADN polymorphe amplifié au hasard (RAPD)

1995 ◽  
Vol 75 (1) ◽  
pp. 221-224 ◽  
Author(s):  
Jean-Guy Parent ◽  
Danièle Pagé
Keyword(s):  
Key Words ◽  
Dna Analysis ◽  
Cultivar Identification ◽  
Random Amplified Polymorphic Dna ◽  
Certification Program ◽  
Strawberry Cultivars ◽  
Selection Of ◽  
Apparently Healthy

Random amplified polymorphic DNA analysis was used to identify and differentiate the 13 strawberry cultivars of Quebec's certification program. DNA extracts from leaves of field plants were analyzed. Young and apparently healthy leaves were chosen to ensure a good quality of DNA. A selection of only two primers, in a test combination, was sufficient to attain the anticipated result. Key words: Strawberry (Fragaria spp.), random amplified polymorphic DNA, RAPD, cultivar identification

scholarly journals 676 PB 160 IDENTIFICATION AND CLASSIFICATION OF PISTACHIO (Pistacia vera L.) CULTIVARS WITH RAPD MARKERS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 529f-529
Author(s):  
J.I. Hormaza ◽  
L. Dollo ◽  
V.S. Polito
Keyword(s):  
Rapd Markers ◽  
Geographical Origin ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Pistacia Vera ◽  
Polymorphic Markers ◽  
Molecular Phenotype ◽  
And Cluster Analysis

The Random Amplified Polymorphic DNA (RAPD) technique was used to characterize 15 cultivars of pistachio (Pistacia vera L.). A total of 37 polymorphic markers were considered in this study. Each cultivar exhibited a unique molecular phenotype and, as a consequence, can be uniquely fingerprinted. A similarity and cluster analysis based on the amplified fragments produced two distinct groups which are consistent with the known geographical origin of the cultivars. Our results suggest that RAPD analysis can provide a new alternative for cultivar identification and classification of pistachio.

scholarly journals Use of Random Amplified Polymorphic DNA Markers for Cultivar Identification in Mint

HortScience ◽  
2001 ◽  
Vol 36 (4) ◽  
pp. 761-764 ◽  
Author(s):  
A.L. Fenwick ◽  
S.M. Ward
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
Dna Markers ◽  
Geographical Origin ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
The United States ◽  
Randomly Amplified Polymorphic Dna ◽  
Commercial Oil

Seventeen mint accessions representing the three species grown for commercial oil production in the United States were characterized using randomly amplified polymorphic DNA (RAPD) analysis. The RAPD profiles readily identified the different Mentha species; calculation of genetic distance, based on the number of shared bands, indicated that M. spicata L. is more closely related to M. × gracilis than to M. × piperita. The RAPD profiles also distinguished among eight peppermint accessions of different geographical origin. However, only limited polymorphism was observed among the most widely grown peppermint and Scotch spearmint cultivars. These results indicate a potential lack of genetic diversity in mint cultivars grown for oil in the United States.

scholarly journals Keragaman genetik Anggrek Grammatophyllum scriptum asal biji dari hasil Kultur In Vitro berdasarkan penanda RAPD

Cassowary ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 10-18
Author(s):  
Zarima Wibawati ◽  
Amelia Sarungallo ◽  
Barahima Abbas
Keyword(s):  
Tissue Culture ◽  
Molecular Markers ◽  
Genetic Distance ◽  
In Vitro Culture ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Polymorphic Band ◽  
Genetic Character ◽  
Orchid Seed

Propagation through tissue culture by using orchid seed as explants will produce a lot of orchid plants. This study aims to measure the genetic character of orchid plantlets that were regenerated from seeds which have been resulted from in vitro culture. The genetic character of the original orchid plants produced from in vitro culture was determined using Random Amplified Polymorphic DNA (RAPD) molecular markers. The results showed that the primers used in the RAPD analysis showed a polymorphic band pattern of 14 DNA bands, with sizes between 500 bp - 8000 bp. The genetic distance of Grammatophyllum scriptum orchids that was regenerated from seeds is between 0.229 and 0.649.  The progenis produced from in vitro culture were clustered into seven groups at a dissimilarity coefficient of 45%.

scholarly journals EFFECT OF CULTIVARS, GROWTH REGULATOR AND SUB-CULTURE ON THE PLANTLET SHOOTS FORMATION AND RAPD ANALYSIS OF STRAWBERRY CULTURES IN VITRO

2019 ◽  
pp. 12-25
Keyword(s):  
Growth Regulator ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Crop Improvement ◽  
Growth Measurement ◽  
Increment Of Growth ◽  
Number Of Leaves ◽  
Improvement Programs ◽  
Maximum Increment

This experiment amid to study the effect of cultivars, growth regulator and number of sub-cultures on the shoots formation of strawberry plantlets during multiplication stage and Random amplified polymorphic DNA (RAPD) analysis of strawberry cultures in vitro during the fourth sub-culture. This experiment included 40 treatments, which were the combination between two strawberry cultivars (Festival and Sweet Charlie), five treatments of growth regulator (BA and GA3) and four number of sub-cultures during shoots formation (multiplication stage). The obtained results showed that, the maximum increment of growth measurement of strawberry plantlets were recorded by Sweet Charlie cultivar .In addition , using ½ MS-medium without supplemented with any growth regulators (BA and GA3) being the superior treatment for increasing both number of leaves per shoot and shoot length. On the other hand, generally, the fourth sub-culture being the most effective treatment on the growth measurement of strawberry plantlets during multiplication stage. Furthermore, Random amplified polymorphic DNA (RAPD) analysis varied according to the two tested cultivars and the type of for production of disease resistant plants and in plant breeding and crop improvement programs (Mohamed, 2003).

Natural and culture-induced genetic variation in plantains (Musa spp. AAB group)

2000 ◽  
Vol 48 (4) ◽  
pp. 493 ◽  
Author(s):  
H. J. Newbury ◽  
E. C. Howell ◽  
Jonathan H. Crouch ◽  
B. V. Ford-Lloyd
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Axenic Culture ◽  
High Rate ◽  
Clear Correlation ◽  
Land Races ◽  
High Level

Random amplified polymorphic DNA (RAPD) analysis of 15, mostly African, plantain land races revealed a very low proportion of polymorphic bands (13 of 276). However, further examination of these 13 marker bands demonstrated that they varied within land races and could not be used to distinguish between land races. In many cases, this could be directly associated with tissue culture treatment of the material. In order to investigate tissue culture effects in more detail, a single meristem of the West African plantain Agbagba was introduced into axenic culture and subjected to three cycles of micropropagation. A total of 48 regenerated plants were established under field conditions and subjected to RAPD analysis. By using 40 arbitrarily selected primers, about 400 bands were scored across this population of in vitro-derived plants. Sixteen of the bands were polymorphic within the population of Agabgba plants, distinguishing 13 genotypes. The pattern of relationships of these genotypes was established by cluster analysis; field characterisation of the plants supported the relationships revealed by RAPD data. The high level of RAPD polymorphism (4% of bands polymorphic), along with a clear correlation between the genotypic classification of individual plants and their tissue culture pedigree, suggests that a substantial amount of genetic variation existed within the original cultured meristem. On this basis, a putative Agbagba meristem representing an apparent sectoral chimera has been constructed. A model is presented that takes account of the persistence and high rate of somaclonal variation and proposes that the mother Agbagba plant comprised a periclinal chimera.

scholarly journals Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

2012 ◽  
Vol 21 (1) ◽  
pp. 63-73 ◽  
Author(s):  
K. Sri Rama Murthy ◽  
R. Kondamudi
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Thin Cell Layers ◽  
In Vitro Plantlets ◽  
Cell Layers ◽  
Extensive Growth

The thin cell layers of nodes and internodes of Ceropegia spiralis L. were cultured on  MS  supplemented with BAP 13.32 µM/l + NAA 0.537 μM/l induced 17.34 ± 0.55 shoots showing extensive growth. Later on the organogenesis was also induced on MS containing BAP 13.32 µM/l + 2, 4-D 1.130 µM/l, whereas the medium with BAP 13.32 µM/l + 2, 4-D 4.52 µM/l has the highest callus producing ability in recalcitrants as well as in normal explants. Shoots developed were rooted best on 0.5 MS with NAA 10.74 μM/l. Optimum shoot and root multiplication was obtained within eight weeks.  In vitro plantlets were successfully weaned and transferred to soil with about 90 per cent survival rate. So far, more than 650 weanlings have been produced successfully and reintroduced into nature for their recovery.     Key words: Ceropegia spiralis, Thin cell layer explants, Recalcitrants, Regeneration   D. O. I. 10.3329/ptcb.v21i1.9564   Plant Tissue Cult. & Biotech. 21(1): 63-73, 2011 (June)

scholarly journals Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa

2004 ◽  
Vol 39 (2) ◽  
pp. 197-199 ◽  
Author(s):  
Rachel Fatima Gagliardi ◽  
Georgia Pereira Pacheco ◽  
Carlos Alberto Oliveira ◽  
Leonardo Alves Carneiro ◽  
José Francisco Montenegro Valls ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Preservation ◽  
Arachis Species ◽  
Embryo Axes ◽  
Genomic Regions ◽  
Regeneration Processes

In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.

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