scholarly journals In vitro propagation of the elite species plant Pluchea lanceolata: Assessment of genetic stability by random amplified polymorphic DNA (RAPD) analysis

2014 ◽  
Vol 13 (25) ◽  
pp. 2500-2505 ◽  
Author(s):  
Nidhi Jain ◽  
N. Verma H.
Keyword(s):  
Genetic Stability ◽  
In Vitro Propagation ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Pluchea Lanceolata

scholarly journals Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2089
Author(s):  
Diaa Mamdouh ◽  
Hany A. M. Mahgoub ◽  
Ahmed M. M. Gabr ◽  
Emad A. Ewais ◽  
Iryna Smetanska
Keyword(s):  
Antioxidant Activity ◽  
Ferulic Acid ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Random Amplified Polymorphic Dna ◽  
Sodium Dodecyl ◽  
Total Phenolic ◽  
Sds Page ◽  
Micropropagated Plants

Lycium schweinfurthii is a Mediterranean wild shrub rich in plant secondary metabolites. In vitro propagation of this plant may support the production of valuable dietary supplements for humanity, introduction of it to the world market, and opportunities for further studies. The presented study aimed to introduce an efficient and reproducible protocol for in vitro micropropagation of L. schweinfurthii and assess the genetic stability of micropropagated plants (MiPs) as well as to estimate phenolic, flavonoid, ferulic acid contents, and the antioxidant activity in leaves of micropropagated plants. Two DNA-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), were used to assess the genetic stability in MiPs. Spectrophotometric analysis was performed to estimate total phenolic and flavonoid contents and antioxidant activity of MiPs leaves, while ferulic acid content was estimated using high-performance thin-layer chromatography (HPTLC). Sufficient shoot proliferation was achieved at MS (Murashige and Skoog) medium supplemented with 0.4 mg L−1 kinetin and rooted successfully on half-strength MS medium fortified with 0.4 mg L−1 Indole-3-butyric acid (IBA). The Jaccard’s similarity coefficients detected in MiPs reached 52%, 55%, and 82% in the RAPD, ISSR, and SDS-PAGE analyses, respectively. In the dried leaves of MiPs, the phenolic, flavonoid, and ferulic acid contents of 11.53 mg gallic acid equivalent, 12.99 mg catechin equivalent, and 45.52 mg were estimated per gram, respectively. However, an IC50 of 0.43, and 1.99 mg mL−1 of MiP dried leaves’ methanolic extract was required to scavenge half of the DPPH, and ABTS free radicals, respectively. The study presented a successful protocol for in vitro propagation of a valued promising plant source of phenolic compounds.

scholarly journals Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa

2004 ◽  
Vol 39 (2) ◽  
pp. 197-199 ◽  
Author(s):  
Rachel Fatima Gagliardi ◽  
Georgia Pereira Pacheco ◽  
Carlos Alberto Oliveira ◽  
Leonardo Alves Carneiro ◽  
José Francisco Montenegro Valls ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Rapd Analysis ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Preservation ◽  
Arachis Species ◽  
Embryo Axes ◽  
Genomic Regions ◽  
Regeneration Processes

In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.

Genetic Stability of Micropropagated Ginger Derived from Axillary Bud through Cytophotometric and RAPD Analysis

2008 ◽  
Vol 63 (9-10) ◽  
pp. 747-754 ◽  
Author(s):  
Sujata Mohanty ◽  
Manoj K. Panda ◽  
Enketeswara Subudhi ◽  
Laxmikanta Acharya ◽  
Sanghamitra Nayak
Keyword(s):  
Dna Content ◽  
Genetic Stability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Nuclear Dna Content ◽  
Basal Medium ◽  
Axillary Buds ◽  
Axillary Bud ◽  
Multiplication Rate

A protocol was developed for the in vitro propagation of ginger (Zingiber officinale) cv. Suprava using dormant axillary buds from unsprouted rhizomes. The dormant axillary buds embedded in the rhizome nodes were induced to sprout when cultured on MS medium supplemented with 6-benzyladenine (BA) alone (1 - 6 mg/l) or with a combination of BA (1 - 6 mg/l) and indole-3-acetic acid (IAA) (0.5, 1 mg/l). In vitro sprouted buds were transferred to the multiplication medium containing various combinations of auxins and cytokinins. MS basal medium supplemented with BA (1 mg/l), IAA (1 mg/l) and adenine sulfate (100 mg/l) was found optimum for the in vitro multiplication of shoots producing (8.2 ± 0.2) shoots from a single explant within 30 days of culture. The multiplication rate remained unchanged in subsequent subcultures. Rooting of shoots occurred in the same multiplication media. Upon transfer of the in vitro culture to ex vitro in pots, 96% of plants survived and established successfully under natural conditions. Tissue culture-raised plantlets of ginger could be conserved in vitro through subculturing at an interval of 4 months. The genetic stability of micropropagated clones was evaluated at regular intervals of 6 months up to 24 months in culture using cytophotometric estimation of 4C nuclear DNA content and random amplified polymorphic DNA (RAPD) analysis. Cytophotometric analysis revealed a unimodal distribution of the DNA content with a peak corresponding to the 4C value (23.1 pg), and RAPD analysis revealed monomorphic bands showing the absence of polymorphism in all fifty regenerants analyzed, thus confirming the genetic uniformity among in vitro grown somaclones of Z. officinale. This study is of commercial significance as axillary bud explants are available throughout the year for initiating a fresh culture of the elite ginger cv. Suprava to be used as a source of true-to-type disease-free planting material thereby minimizing the adverse effect of repeated subculturing from the same explant source.

In vitro propagation and assessment of genetic stability of micropropagated Samanea saman (rain tree) using microsatellite markers

2013 ◽  
Vol 35 (8) ◽  
pp. 2467-2474 ◽  
Author(s):  
Sampath Kasthurirengan ◽  
Lifen Xie ◽  
Chun Hong Li ◽  
Yok King Fong ◽  
Yan Hong
Keyword(s):  
Microsatellite Markers ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Samanea Saman

In vitro propagation of endangered Mammillaria genus (Cactaceae) species and genetic stability assessment using SSR markers

2018 ◽  
Vol 54 (5) ◽  
pp. 518-529 ◽  
Author(s):  
Jesús Omar Lázaro-Castellanos ◽  
Martín Mata-Rosas ◽  
Dolores González ◽  
Salvador Arias ◽  
Frédérique Reverchon
Keyword(s):  
Ssr Markers ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Stability Assessment

In vitro propagation of Plum (Prunus salicina) cv. ‘Santa Rosa’ and assessment of genetic stability using RAPD markers

2018 ◽  
Vol 23 (1) ◽  
pp. 161-168 ◽  
Author(s):  
Manisha Thakur ◽  
Madhvi Soni ◽  
Dharam Paul Sharma ◽  
Manu Vivek ◽  
Vishal Sharma
Keyword(s):  
Genetic Stability ◽  
In Vitro Propagation ◽  
Rapd Markers ◽  
Prunus Salicina

A comparison of genetic stability in tea [Camellia sinensis (L.) Kuntze] plantlets derived from callus with plantlets from long-term in vitro propagation

2019 ◽  
Vol 138 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Lidiia Samarina ◽  
Maya Gvasaliya ◽  
Natalia Koninskaya ◽  
Ruslan Rakhmangulov ◽  
Alexander Efremov ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Genetic Stability ◽  
In Vitro Propagation
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