DISCRIMINATION OF CULTIVARS OF LENTIL (Lens culinaris Medic.) BY ELECTROPHORESIS OF SEED PROTEINS

1989 ◽  
Vol 69 (1) ◽  
pp. 243-246 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
K. W. CLARK
Keyword(s):  
Gel Electrophoresis ◽  
Lens Culinaris ◽  
Seed Protein ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel

Discrimination of lentil cultivars was achieved by analysis of seed protein by two types of polyacrylamide gel electrophoresis. Cultivars of lentil were discriminated by the presence or absence of diagnostic bands. Electrophoregrams of six seed lots of the cultivar Eston were identical and unaffected by growing conditions.Key words: Lens culinaris Medic, seed proteins, polyacrylamide gel electrophoresis, cultivar identification

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

Inheritance and distribution of variation at four avenin loci in North American oat germ plasm

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 416-424 ◽  
Author(s):  
E. Souza ◽  
M. E. Sorrells
Keyword(s):  
North American ◽  
Gel Electrophoresis ◽  
Germ Plasm ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
High Association ◽  
Electrophoretic Mobilities ◽  
Oat Cultivars ◽  
Taxonomic Studies

Avenins (prolamines of the Avena genus) have been shown to be useful in taxonomic studies and cultivar identification; specific allelic identification could assist in these types of studies as well as providing a basis for future linkage and gene mapping studies. The avenin patterns produced by nondenaturing polyacrylamide gel electrophoresis were compared in 70 North American oat cultivars and germ plasms. Populations of F2 progeny were subsequently evaluated to test for allelism of proteins found to be noncoincident in the survey of homozygous cultivars. A minimum of four loci (Av1, Av2, Av3, and Av4) were found to possess alternate alleles with distinctive electrophoretic mobilities. Segregation of 10 alternate alleles were observed in studies of F2 progeny: four for Av1, and two each for the other three loci. Additional variation found among the surveyed cultivars suggested at least two additional electrophoretically variant polypeptides. Several of the alleles were found to be associated with cultivars from specific geographic regions. Two examples were (i) the near exclusive association of the Av10.76 allele with Canadian cultivars and (ii) the high association of the Av10.58 allele with fall-planted cultivars. Fifty percent (SE ± 10.7%) of the fall-planted cultivars have the Av40.58 allele compared with 27.1% (SE ± 8.8%) of spring-planted cultivars.Key words: avenins, prolamines, polyacrylamide gel electrophoresis, linkage.

The comparison of seed protein patterns within the genusArachis by polyacrylamide gel electrophoresis

1983 ◽  
Vol 25 (4) ◽  
pp. 266-273 ◽  
Author(s):  
Eva Klozová ◽  
Jiřina Švachulová ◽  
J. Smartt ◽  
E. Hadač ◽  
Věra Turková ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Patterns

IDENTIFICATION OF CICER MILKVETCH CULTIVARS USING SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS

1983 ◽  
Vol 63 (4) ◽  
pp. 1087-1090 ◽  
Author(s):  
ROBERT K. EVANS ◽  
ROLLIN H. ABERNETHY
Keyword(s):  
Banding Pattern ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legumes ◽  
Electrophoretic Method ◽  
Stand Establishment ◽  
Astragalus Cicer ◽  
Varietal Purity

With the release of Monarch cicer milkvetch (Astragalus cicer L.), which has greatly facilitated stand establishment when compared to older cultivars, the desirability of a method for identifying seed of cultivars was recognized. An SDS-polyacrylamide gel electrophoretic method was developed to differentiate cicer milkvetch cultivars. Densitometric scanning of the resultant polypeptide banding pattern on the polyacrylamide gel provided identification of seed lots of the cultivars Oxley, Lutana and Monarch produced in several different years and locations.Key words: Astragalus cicer L., forage legumes, seed proteins, varietal purity

scholarly journals Polyacrylamide gel electrophoresis of soybean seed proteins

2015 ◽  
Vol 47 (4) ◽  
pp. 371-378 ◽  
Author(s):  
W. Lassocińsk ◽  
J. S. Knypl
Keyword(s):  
Glycine Max ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Soluble Proteins ◽  
Polyacrylamide Gel ◽  
Major Protein ◽  
Molecular Weights ◽  
Minor Protein

Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were detected under dissociating conditions. Molecular weights of three major protein fractions in PAGE SDS were determined for around 18 500, 36 000 and 80 000 daltons.

scholarly journals THE USE OF SEED PROTEIN BANDING PATTERNS FOR IDENTIFICATION OF PASTURE CULTIVARS

1988 ◽  
pp. 87-91
Author(s):  
M.B. Forde ◽  
S.E. Gardiner
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Subterranean Clover ◽  
Seed Storage Proteins ◽  
Polyacrylamide Gel ◽  
Banding Patterns ◽  
Seed Certification ◽  
Plant Variety

Because of the growing number of pasture cultivars used in NZ and the difficulty of reliably separating cultivars of the same species by morphological characters, seed protein banding patterns have become a useful supplementary means of cultivar identification for the purposes of seed certification and plant variety rights applications. Sodium dodecylsufphate polyacrylamide gel electrophoresis of proteins extracted from ground seed samples produces distinctive patterns of bands representing seed storage proteins of different molecular weights. The procedure can be carried out in two days using viable or dead seed, and the results are not affected by site and season of growth. Although individual seeds of outbreeding species such as perennial ryegrass and white clover produce different banding patterns, the combined population representing the cultivar remains constant unless there has been genetic shift during seed multiplication. Speckes for whrch this procedure is being successfully used include the ryegrasses and fescues, browntop, cocksfoot, bromes, red and white clovers, subterranean clover, serradella and lotus. Even cultrvars as closely related as Nui and Ellett ryegrasses and Huia and Pitau white clovers can be separated by careful work. Because of minor technical differences between runs, all cultivars to be compared must be run on the same gel. Keywords: Seed certification, Plant variety rights, sodium dodecylsulphate polyacrylamide gel electrophoresis.

scholarly journals Isolation of Some Platelet Membrane Glycoproteins

1977 ◽  
Author(s):  
K.J. Clemetson ◽  
S.L. Pfueller ◽  
A. Sturk ◽  
E.F. Lüscher ◽  
C.S.P. Jenkins
Keyword(s):  
Gel Electrophoresis ◽  
Wheat Germ ◽  
Lens Culinaris ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Platelet Membrane ◽  
Buffer System ◽  
Membrane Glycoproteins ◽  
Abrus Precatorius ◽  
Differential Binding

The platelet is surrounded by a pronounced glycocalix formed by carbohydrate moieties of the membrane glycoproteins. The number of glycoproteins of the outer platelet membrane is greater in number than had previously been reported : when solubilized membranes are analyzed by SDS-polyacrylamide gel electrophoresis the number of separated carbohydrate entities was found not only to be dependant on the concentration of acrylamide and of bisacrylamide used but also on the buffer system employed.The major platelet membrane glycoproteins have been solubilized and subjected to affinity chromatography on the lectins from Lens culinaris, wheat germ and Abrus precatorius. SDS-polyacrylamide gel electrophoresis in the presence and absence of a reducing agent together with the differential binding of the lectins to the glycoproteins permitted the distinction of at least seven glycoprotein entities. Using combinations of lectin columns, two platelet membrane glycoproteins have been isolated and others have been greatly purified.

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