EFFETS D’UNE MORPHACTINE, LE CHLORFLURENOL IT-3456, SUR LA CROISSANCE ET LES ECHANGES GAZEUX DU TOURNESOL KRASNODARETS

1985 ◽  
Vol 65 (3) ◽  
pp. 533-541 ◽  
Author(s):  
DANIEL LORD ◽  
HENRI-P. THERRIEN ◽  
PIERRE-A. DUBÉ

A morphactin, the chlorflurenol IT-3456 (CFL), was applied to sunflower in order to evaluate its effects on growth, chlorophyll content and rates of photosynthesis, respiration and transpiration. At the concentrations used in the experiments (50, 100, 200, 300 ppm) growth inhibition and morphogenic effects of CFL were evident. However, this morphactin did not significantly decrease the fresh and dry weights of plants. The treatment at the lowest concentration of CFL (50 ppm) stimulated chlorophyll a and b synthesis while the other treatments had no effect. An open system to measure the gas exchanges of sunflower leaves was used to calculate the rates of photosynthesis, respiration and transpiration. Rates of these processes were not significantly affected by the application of different concentrations of CFL. It is suggested that the growth inhibition of plants treated with CFL owes more to an effect at the partition level of the new photosynthates rather than by an alteration of the photosynthesis mechanisms.Key words: Morphactin, chlorflurenol, photosynthesis, respiration, transpiration, sunflower

2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat

Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants) Muhammad Tawary1*), Julius Pontoh1), Lydia I.Momuat1)1)Jurusan Kimia, FMIPA UNSRAT Manado*Email korespondensi: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kelapa banyak dibudidayakan sebagai tanaman pertanian. Tanaman kelapa memiliki nilai ekonomi yang tinggi karena hampir semua bagian tanaman kelapa memiliki manfaat ekonomis. Penelitian bertujuan untuk  mengembangkan metode analisis klorofil pada daun dan menentukan bagian anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada setiap bagian anak daun tanaman kelapa. Kandungan klorofil a pada posisi tengah anak daun kelapa (763.19 µmol/m2) dan kandungan klorofil b (196.22 µmol/m2). Kandungan klorofil a dan b pada setiap bagian anak daun dan posisi kanan dan kiri anak daun memiliki nilai yang relatif sama.Kata kunci: Kelapa, klorofil a, klorofil b Abstract Many coconut plants are cultivated as agricultural crops. Coconut plants have high economic value because almost all parts of coconut plants have economic benefits. The study aims to develop a method of chlorophyll analysis on leaves and determine the part of leaflets which have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. The content of chlorophyll a in leaves varies with each part of the leaves of the coconut plant. Chlorophyll a content in the middle position of coconut leaf child (763.19 µmol / m2) and chlorophyll b content (196.22 µmol / m2). The content of chlorophyll a and b in each part of the leaf child and the right and left positions of the leaves have relatively the same value.Keywords: Coconut, Chlorophyll a, Chlorophyll b


2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.


2016 ◽  
Vol 24 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Matúš Peško ◽  
Marianna Molnárová ◽  
Agáta Fargašová

AbstractPresented study evaluates effects of various Sb(III) concentrations on tomato plants (Solanum lycopersicum) cultivated hydroponically. Visual symptoms of antimony toxicity were observed only at two highest applied concentrations (50 and 100 mg/L). Dry weight of aboveground parts decreased significantly in variants treated with 25, 50 and 100 mg/L Sb(III), by ~12, 35 and 65 %, respectively, in comparison to the control. Statistically significant decrease of chlorophyll a and b was observed only after application of two highest studied concentrations 50 and 100 mg/L Sb(III). On the other hand concentration of total carotenoids in leaves rose with increasing external Sb(III) concentration. High concentrations (50 and 100 mg/L) of Sb(III) in nutrient solution caused that protein content in leaves dropped by ~20 and 39% relative to control. Accumulation of antimony in roots was about 5- (10 mg/L) to 27-times (25 mg/L) greater than that in shoots. The highest BAF factor value determined for shoots was ~55 at 10 mg/L Sb(III) and for roots it was ~821 at 50 mg/L Sb(III). Translocation factor values were in whole studied concentration range 5 – 100 mg/L Sb(III) < 1. The most effective translocation of antimony from roots to shoots was observes for variants treated with 10 mg/L of Sb(III).


Author(s):  
BILLY THEODORUS WAGEY

Chlorophyll content of seagrass leaves varies among species and habitats byat least a factor of five, and shows plastic responses to seasonal variations in light availability as well as direct experimental manipulation. This study generally attempted to observe the relative amount of chlorophyll a, b content of Haloduleuninervis and Halodule pinifolia in Central Visayas, Philippines in relation tothese environmental factors which are: exposure, density, temperature, and lightintensity. Chlorophyll was determined using the equation of Jeffrey and Humprey(1975) whereas data gathered went through one way ANOVA followed byTukey’s Test. Significant variations were found in most photosynthetic parametersmeasured in this study. Overall, H. uninervis and H. pinifolia seems to obtainoptimal photosynthetic performances over a broad range of irradiances. Mainly,genus Halodule can be seen as a highlight species, which has also the ability to acclimate to low irradiances. in general, the result indicated that seagrasses tend to decrease the ratio of chlorophyll a:b by increasing the amount of chlorophyllb to compensate for the decline in light intensity in subtidal areas. Therefore, H.uninervis and H. pinifolia seem to have the ability of adjusting their chlorophyllcontent in performing optimal photosynthetic activity over a broad range of irradiance regimes.Keywords: Marine Ecology, chlorophyll content, seagrass, Halodule, experimental design,Philippines


e-CUCBA ◽  
2021 ◽  
Vol 15 (8) ◽  
pp. 44-52
Author(s):  
Sánchez-Velázquez Emmanuel ◽  
◽  
Rodríguez-Sahagún AracelI ◽  
Acevedo-Hernández Gustavo J ◽  
Rayn C Aarland ◽  
...  

The use of different light sources in the micropropagation of plant species has been studied since the 1970s. At present, studies are being carried out not only to increase production, but to greatly reduce the energy demand of the lamps that are used for this purpose. In the present work, light sources with different wavelengths in fluorescent lamps and LED light were used to test the morphological and phytochemical response of Mexican origanumplants. A similar response was obtained regarding the production of axillary buds and leaves with the use of broad spectrum light and LED light. On the other hand, the main photosynthetic pigments (chlorophyll a and b and carotenoids) are also produced in a similar way when this plant is propagated under broad spectrum light and LED light.


2015 ◽  
Vol 21 (1-2) ◽  
Author(s):  
M. Ördögh

During in vitro multiplication of Nidularium ‘Kertész Jubileum’, 20 g/l sucrose, 5 g/l agar, 100 mg/l inositol, and different concentrations of benzyladenine (BA), benzyladenine-riboside (BAR), kinetin (KIN), meta-topolin (mT) were added to the MKC (Knudson, 1946) basal medium. Furthermore, 0.1 mg/l naphthaleneacetic acid was used to every medium. Number of shoots, length of leaves, number and length of roots, chlorophyll (a+b) content were examined and evaluated with Ropstat statistical software. As compared to the other cytokinin, significantly most shoots were obtained in the case of applying BA. Increasing of BA-concentration (as far as 2 mg/l) enhanced shoot number (from 10.92 to 19.26) but 4 mg/l BA resulted only 6.63 shoot. The less efficient cytokinin was KIN, in most cases no more than about 2 shoot was achieved. Regarding the length of leaves, the higher level of BA effected averagely the shorter leaves (from 24,46 to 7.31 mm). KIN effected significantly the longest leaves (43.4-61.29) in inverse proportion to the concentration. The same cytokinin resulted the most (and the longest) roots with the highest rooting percentages, but more KIN decreased the number and length of roots (from 7.95 to 4.4 and from 38.49 to 22.73 mm). There were no definite correlation between cytokinin concentration and chlorophyll (a+b) content, but the highest doses resulted decreasing (except of meta-topolin which leads to the lowest values). Summarizing, BAR effected the highest contents (mostly more than 1400 μg/g), particularly in the case of 1 mg/l (1807.3 μg/g).


2019 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat

Analsis Kandungan Klorofil pada Beberapa Jenis Tanaman Palma(Analysis of Chlorophyll Content in Several Types of Palm Plants) Muhammad Tawary1)*, Julius Pontoh1), Lydia Momuat1)1)Jurusan Kimia, FMIPA Universitas Sam Ratulangi,  Manado 95115*Email korespondensi: [email protected] Diterima  15 Februari 2019, diterima untuk dipublikasi 28 Februari  2019 Abstrak Tanaman palma banyak dibudidayakan sebagai tanaman pertanian. Ada beberapa jenis tanaman palma seperti kelapa, kelapa sawit, dan aren. Penelitian ini bertujuan untuk mengembangkan metode analisis klorofil pada daun dan menentukan posisi anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada ketiga jenis tanaman palma, yaitu pada daun kelapa berkisar antara 586.56 sampai 835.75 µmol/m2, daun kelapa sawit 674.47 µmol/m2 sampai 982.98 µmol/m2, dan pada daun aren 1300.82 µmol/m2 sampai 1449.65 µmol/m2. Klorofil b pada daun kelapa berkisar antara 160.79 µmol/m2 sampai 237.86 µmol/m2, daun kelapa sawit 191.36 µmol/m2 sampai 315.63 µmol/m2, dan daun aren 423.29 sampai 543.19 µmol/m2. Kandungan klorofil a pada setiap posisi anak daun ketiga jenis tanaman palma memiliki nilai yang hampir sama sedangkan kandungan klorofil b pada setiap posisi anak daun pada ketiga jenis tanaman palma memiliki nilai yang sedikit berbeda. Kandungan klorofil a dan b pada setiap bagian anak daun aren memiliki nilai yang hampir sama begitupun dengan kelapa dan kelapa sawit. Kandungan klorofil a pada posisi kanan dan kiri daun aren memiliki nilai yang sedikit berbeda sedangkan pada kelapa dan kelapa sawit memiliki nilai yang hampir sama. Kandungan klorofil b pada ketiga jenis tanaman palma tidak memiliki perbedaan.Kata kunci: Palma, klorofil, absorbansi, spektrofometer Abstract Many palma plants are cultivated as agricultural crops. Several types of palm plants, for example coconut, oil palm, and sugar palm. This research was conducted to develop a method of chlorophyll analysis on leaves and determine the position of leaves children who have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. TheThe content of chlorophyll contained in coconut leaves varies, namely containing chlorophyll content contained in coconuts varies, which contains 586.56 µmol/m2 to 835.75 μmol / m2, in oil palm leaves 674.47 µmol/m2 to 982.98 μmol / m2, and in palm leaves 1300.82 to 1449.65 μmol / m2. Whereas for chlorophyll b found in coconut leaves varies around 160.79 µmol/m2 to 237.86 µmol / m2, on palm oil leaves 191.36 µmol/m2 to 315.63 µmol / m2, and on palm leaves 423.29 µmol/m2  to 543.19 µmol / m2. The content of chlorophyll a at each position of leaflets on leaves of various types of palma plants has almost the same value while the content of chlorophyll b at each position of leaflets on the leaves of various types of palma plants has slightly different values. The content of chlorophyll a and b in each part of palm leaves has almost the same value as well as coconut and oil palm. Chlorophyll a content in the right and left position of palm leaves has slightly different values while coconut and oil palm have almost the same value. Chlorophyll b content in all three types of palma plants has no difference.Keywords: Palma, chlorophyll, absorbance, spectrophotometer


2015 ◽  
pp. 133-136
Author(s):  
Árpád Szalacsi ◽  
Gergely Király ◽  
Szilvia Veres

Photosynthetic parameters of English oak (Quercus robur L.) as a member of Querco robori-Carpinetum were investigated in two different habitat in terms of gap forest management: in the gap and in the host forest. The artifical opening process of the forest resulted in more light for growing saplings and need for acclimatization. Photosynthesis is one of the most important way for plant life and plant production. In the centre of photosynthetic efficiency the quality and quantity traits of photosynthetic pigments are standing. During our work some photosynthetic parameters of plants (in the gap and in the forest as well) were measured: relative chlorophyll content as SPAD index, chlorophyll a and b content, total chlorophyll content and ratio of chlorophyll a and b. Based on our results no significant differences among our data in early spring. Although, during the summer significant differences occurred between the measured values in the gap and in the forest area. Lower total chlorophyll content was experienced in the gap, than in the forest area due to the lower chlorophyll-b content. Because of the high light intensity higher chla/chlb ratio was measured in the gap. The lower chlorophyll contents of gap habitat may have a part of the acclimatization process of photosynthetic apparatus against high light stress, which can determinate the survival chance of individual.


2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Olivia P. G. Lawendatu ◽  
Julius Pontoh ◽  
Vanda Kamu

ABSTRAKTelah dilakukan penelitian tentang analisis kandungan klorofil dari berbagai posisi daun dan anak daun dari tanaman aren. Daun aren diambil pada posisi atas, tengah, dan bawah dari tajuk tanaman. Sampel anak daun  dibagi 3 posisi yaitu atas, tengah, dan bawah. Ekstraksi dilakukan dengan methanol dan penetuan klorofil pada daun dilakukan dengan spektofotometer pada panjang gelombang 665 nm dan 652 nm untuk masing-masing klorofil a dan klorofil b. Kandungan klorofil a dan b  yang terdapat pada sampel daun aren yang dihitung berdasarkan 3 unit satuan µg/mL, mg/g  dan µmol/m2. Kandungan klorofil a dan b pada sampel pohon aren tertinggi terletak pada posisi kiri daun, pada posisi daun terletak pada daun atas, pada posisi anak daun terletak pada posisi ujung anak daun. Ratio kandungan klorofil pada sampel daun aren berkisar 1,30-6,72. ABSTRACTHas conducted research on the analysis of chlorophyll content from various positions of leaves and leaves of palm plants. Leaves are taken in the top, middle and bottom positions of the plant canopy. Samples of leaflets were divided into 3 positions namely top, middle and bottom. Extraction was carried out with methanol and the determination of chlorophyll in the leaves was carried out by spectrophotometer at wavelengths of 665 nm and 652 nm for chlorophyll a and chlorophyll b, respectively. The chlorophyll and b content in palm leaf samples are calculated based on 3 units of μg/mL, mg/g, and µmol/m2. The content of chlorophyll a and b in the highest palm tree sample is located in the left position of the leaf, the position of the leaf is placed on the top of the leaf, the position of the leaflets is placed in the tip of the leaflets. The ratio of chlorophyll content in sugar palm leaf samples in the setting is 1.30-6.72. 


2015 ◽  
Vol 28 (2) ◽  
pp. 177-195 ◽  
Author(s):  
Jan Tonecki

The results of investigations on the effect of 2,4,5-T (butyl ester of 2,4,5-trichlorophenoxy acetic acid) and dalapon (sodium salt of 2,2-dichloropropionic acid) on chlorophyll content and rate of respiration in needles of spruce seedlings, treated with different concentrations of both herbicides before bud development and during bud formation' are presented. 2,4,5-T and dalapon decreased chlorophyll a and b content in the needles. The chlorophyll a : b ratio increased after treatment with these herbicides. The lower concentrations tested produced a higher value of this coefficient. Depending on concentration both herbicides caused disturbances in respiration of needles of seedlings treated during bud formation. Stimulation of respiration by both herbicides in the newly formed needles of seedlings treated before bud development was observed.


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