IN VITRO PROPAGATION OF OTTAWA 3 APPLE ROOTSTOCK

1983 ◽  
Vol 63 (1) ◽  
pp. 183-188 ◽  
Author(s):  
E.-C. PUA ◽  
CALVIN CHONG ◽  
G. L. ROUSSELLE
Keyword(s):  
Culture Medium ◽  
Agar Medium ◽  
Shoot Proliferation ◽  
Normal Growth ◽  
Naphthalene Acetic Acid ◽  
Shoot Cultures ◽  
Apple Rootstock ◽  
In Vitro Proliferation ◽  
In Vitro Shoot Proliferation

Methods were developed for obtaining normal shoot cultures and for rapid in vitro proliferation and rooting of Ottawa 3 apple rootstock from meristem tips. The presence of naphthalene acetic acid (NAA) and benzyladenine (BA), both at concentrations of either 0.5 or 1.0 mg/L in the culture medium, was most effective for in vitro shoot proliferation, but growth was abnormal. Normal growth was achieved when shoots were cultured with a combination of 0.5 mg/L NAA, 0.5 mg/L BA and 5.0 mg/L gibberellic acid. One hundred percent rooting was achieved after 2 wk on agar medium supplemented with 6.25 mg/L indole butyric acid.Key words: Tissue culture, Malus, meristem cloning, growth regulators

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Pyroligneous Acid Improves In Vitro Rooting of Japanese Pear Cultivars

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 194-195 ◽  
Author(s):  
Masanori Kadota ◽  
Takashi Hirano ◽  
Kiyotoshi Imizu ◽  
Yoshiji Niimi
Keyword(s):  
Root Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
In Vitro Rooting ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Shoot Cultures ◽  
Pyroligneous Acid ◽  
In Vitro Shoot Proliferation

Effects of PA on in vitro shoot proliferation and root formation were investigated using shoot cultures of three Japanese pear (Pyrus pyrifolia Nakai) cultivars. PA inhibited shoot multiplication and promoted initiation and development of roots in the cultured shoots of three cultivars, resulting in increasing the proportion of rooted shoots. Chemical name used: pyroligneous acid (PA).

scholarly journals Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

2018 ◽  
Vol 12 (2) ◽  
pp. 405-415
Author(s):  
Paulo Mauricio Centenaro Bueno ◽  
Luiz Antonio Biasi ◽  
Mauro Brasil Dias Tofanelli
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Simple Protocol ◽  
Growth Room ◽  
In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

scholarly journals Biochemical characterization of systemic bacteria in bananas, sensitivity to antibiotics and plant phytotoxicity during shoot proliferation

2016 ◽  
Vol 38 (2) ◽  
pp. 193 ◽  
Author(s):  
Janiffe Peres de Oliveira ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Nalidixic Acid ◽  
Culture Medium ◽  
Inhibitory Concentration ◽  
Biochemical Characterization ◽  
Shoot Proliferation ◽  
In Vitro Proliferation ◽  
Bacterial Sensitivity

The objective of this work was to characterize the biochemically systemic bacterial isolated from banana plants, to evaluate the bacterial sensitivity to antibiotics, and to determine the phytotoxicity of banana shoots during in vitro proliferation. Systemic bacteria belonging to the Klebsiella and Aeromonas genera were isolated from the “Maravilha” (FHIA 01 AAAB), “Preciosa” (PV 4285 AAAB) and “Thap Maeo” (AAB) varieties and were then characterized. Tests of shoot sensitivity to antibiotics were performed, and the minimum inhibitory concentration (MIC) and phytotoxic effects of selected antibiotics to plants were determined. Among the 20 antibiotics evaluated, the strains showed sensitivity to cefaclor, cefalexin, cefalotin, nalidixic acid, chloramphenicol, and vancomycin. However, during MIC determination, the best results were obtained with cefaclor, vancomycin or nalidixic acid alone in concentrations ranging from 512 to 1,024 mg L-1. In culture medium, cefaclor at 1,024 mg L-1 was the only antibiotic to affect the multiplication and the shoot survival in culture. 

scholarly journals In Vitro Shoot Proliferation and Root Induction of Shoot Tip Explants from Mature Male Plants of Casuarina cunninghamiana Miq.

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 797-800 ◽  
Author(s):  
Xiuli Shen ◽  
William S. Castle ◽  
Frederick G. Gmitter
Keyword(s):  
Acetic Acid ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Casuarina Cunninghamiana ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation

This study examined the effects of plant growth regulators, explant types, and their orientations on in vitro shoot proliferation of Casuarina cunninghamiana Miq. and also the subsequent rooting ability of shoots. Results showed that shoot proliferation occurred only in shoot tip explants cultured vertically on Murashige and Skoog (MS) medium supplemented with 2 or 4 μM thidiazuron (TDZ). Neither 6-benzylaminopurine alone nor in a combination with 1-naphthalene acetic acid (NAA) or gibberellic acid had any effect on shoot proliferation. TDZ at 4 μM resulted in the greatest percentage of axillary bud sprouting (70%) and mean number of sprouts per explant (2.3). Additionally, no shoot proliferation was observed from detipped or single-node explants or from horizontally placed shoot tip explants when cultured on the same TDZ-containing medium. The induced shoots produced adventitious roots on MS medium supplemented with 2.5, 5, or 10 μM indole-3-butyric acid (IBA), not with indole-3-acetic acid and NAA. Although the mean number of roots per explant was not significantly different between 2.5 and 5 μM IBA, the highest rooting percentage (68%) and mean length of roots per explant (0.7 cm) was achieved at 5 μM IBA. The current study provided preliminary information toward commercial in vitro propagation of Casuarina cunninghamiana male plants.

scholarly journals Effect of Media Types and Carbon Sources on In vitro Shoot Proliferation of Spilanthes acmella (L.) Murray

2014 ◽  
Vol 23 (2) ◽  
pp. 283-288
Author(s):  
Veenu Joshi ◽  
S. K. Jadhav
Keyword(s):  
Carbon Sources ◽  
Shoot Proliferation ◽  
Spilanthes Acmella ◽  
Media Types ◽  
In Vitro Shoot Proliferation

D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17529Plant Tissue Cult. & Biotech. 23(2): 283-288, 2013  (December)

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

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