Changes in nutrient composition and pH of the culture medium during in vitro shoot proliferation of crabapple and pear

1987 ◽  
Vol 11 (3) ◽  
pp. 209-220 ◽  
Author(s):  
Suman Singha ◽  
Gene H. Oberly ◽  
Edwin C. Townsend
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Nutrient Composition ◽  
In Vitro Shoot Proliferation

scholarly journals Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

2018 ◽  
Vol 12 (2) ◽  
pp. 405-415
Author(s):  
Paulo Mauricio Centenaro Bueno ◽  
Luiz Antonio Biasi ◽  
Mauro Brasil Dias Tofanelli
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Simple Protocol ◽  
Growth Room ◽  
In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

IN VITRO PROPAGATION OF OTTAWA 3 APPLE ROOTSTOCK

1983 ◽  
Vol 63 (1) ◽  
pp. 183-188 ◽  
Author(s):  
E.-C. PUA ◽  
CALVIN CHONG ◽  
G. L. ROUSSELLE
Keyword(s):  
Culture Medium ◽  
Agar Medium ◽  
Shoot Proliferation ◽  
Normal Growth ◽  
Naphthalene Acetic Acid ◽  
Shoot Cultures ◽  
Apple Rootstock ◽  
In Vitro Proliferation ◽  
In Vitro Shoot Proliferation

Methods were developed for obtaining normal shoot cultures and for rapid in vitro proliferation and rooting of Ottawa 3 apple rootstock from meristem tips. The presence of naphthalene acetic acid (NAA) and benzyladenine (BA), both at concentrations of either 0.5 or 1.0 mg/L in the culture medium, was most effective for in vitro shoot proliferation, but growth was abnormal. Normal growth was achieved when shoots were cultured with a combination of 0.5 mg/L NAA, 0.5 mg/L BA and 5.0 mg/L gibberellic acid. One hundred percent rooting was achieved after 2 wk on agar medium supplemented with 6.25 mg/L indole butyric acid.Key words: Tissue culture, Malus, meristem cloning, growth regulators

scholarly journals Effect of Media Types and Carbon Sources on In vitro Shoot Proliferation of Spilanthes acmella (L.) Murray

2014 ◽  
Vol 23 (2) ◽  
pp. 283-288
Author(s):  
Veenu Joshi ◽  
S. K. Jadhav
Keyword(s):  
Carbon Sources ◽  
Shoot Proliferation ◽  
Spilanthes Acmella ◽  
Media Types ◽  
In Vitro Shoot Proliferation

D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17529Plant Tissue Cult. & Biotech. 23(2): 283-288, 2013  (December)

scholarly journals Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

3 Biotech ◽  
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mouaad Amine Mazri ◽  
Reda Meziani ◽  
Jamal El Fadile ◽  
Az-eddine Ezzinbi
Keyword(s):  
Date Palm ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
In Vitro Shoot Proliferation

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals In Vitro Shoot Proliferation and Rooting of Several Japanese Persimmon Cultivars

1989 ◽  
Vol 58 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Hideki MURAYAMA ◽  
Ryutaro TAO ◽  
Tatsumi TANAKA ◽  
Akira SUGIURA
Keyword(s):  
Shoot Proliferation ◽  
Japanese Persimmon ◽  
In Vitro Shoot Proliferation
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