BARLEY CULTIVAR IDENTIFICATION BY ELECTROPHORETIC ANALYSIS OF HORDEIN PROTEINS II. CATALOGUE OF ELECTROPHOREGRAM FORMULAE FOR CANADIAN-GROWN BARLEY CULTIVARS

1981 ◽  
Vol 61 (4) ◽  
pp. 859-870 ◽  
Author(s):  
B. A. MARCHYLO ◽  
D. E. LABERGE
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Analysis ◽  
Polyacrylamide Gel ◽  
Barley Cultivar ◽  
Relative Mobility ◽  
Malting Barley ◽  
Barley Cultivars ◽  
Acid Ph

Hordein proteins, extracted from 62 Canadian-grown two- and six-rowed barley cultivars and six American-grown six-rowed barley cultivars, were separated by polyacrylamide gel electrophoresis at acid pH. Band mobilities were shown to be reproducible within ± 0.005 relative mobility units. Hordein electrophoregram formulae were determined and catalogued for the 68 barley cultivars analyzed. Twenty-five of the cultivars produced unique electrophoregram formulae. The remaining 43 cultivars were divided into 17 groups, each containing between two and eight indistinguishable cultivars. Further analysis of electrophoregram formulae for Canadian-grown barley cultivars revealed that the majority of malting barley cultivars were distinguishable from feed barley cultivars. The usefulness of the acidic PAGE procedure for the identification of Canadian-grown barley cultivars is evaluated.

BARLEY CULTIVAR IDENTIFICATION BY ELECTROPHORETIC ANALYSIS OF HORDEIN PROTEINS: I. EXTRACTION AND SEPARATION OF HORDEIN PROTEINS AND ENVIRONMENTAL EFFECTS ON THE HORDEIN ELECTROPHOREGRAM

1980 ◽  
Vol 60 (4) ◽  
pp. 1343-1350 ◽  
Author(s):  
B. A. MARCHYLO ◽  
D. E. LaBERGE
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Analysis ◽  
Western Canada ◽  
Barley Cultivar ◽  
Barley Cultivars ◽  
Extraction And Separation ◽  
Acid Gel

Reproducible hordein electrophoregrams were obtained when hordeins, extracted from Canadian-grown barley cultivars, were subjected to polyacrylamide gel electrophoresis at acid pH (acid-gel PAGE). The hordeins were extracted at room temperature into a solution of 55% (vol/vol) isopropanol containing 2% (vol/vol) monothioglycerol. Extraction of hordein at high temperature (60 °C) produced significant reduction in the number of protein bands available for cultivar identification. Alkylation of the reduced hordeins, prior to acid-gel PAGE, did not improve the resolution or appearance of the hordein electrophoregrams. The effect of environment on the hordein electrophoregram was studied. Four barley cultivars were grown at eight locations in Western Canada during 2 successive years. Hordein electrophoregrams were qualitatively independent of growth location, year of growth and protein content. These results suggest that acid-gel PAGE should prove useful as a technique for barley cultivar identification.

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

Inheritance and distribution of variation at four avenin loci in North American oat germ plasm

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 416-424 ◽  
Author(s):  
E. Souza ◽  
M. E. Sorrells
Keyword(s):  
North American ◽  
Gel Electrophoresis ◽  
Germ Plasm ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
High Association ◽  
Electrophoretic Mobilities ◽  
Oat Cultivars ◽  
Taxonomic Studies

Avenins (prolamines of the Avena genus) have been shown to be useful in taxonomic studies and cultivar identification; specific allelic identification could assist in these types of studies as well as providing a basis for future linkage and gene mapping studies. The avenin patterns produced by nondenaturing polyacrylamide gel electrophoresis were compared in 70 North American oat cultivars and germ plasms. Populations of F2 progeny were subsequently evaluated to test for allelism of proteins found to be noncoincident in the survey of homozygous cultivars. A minimum of four loci (Av1, Av2, Av3, and Av4) were found to possess alternate alleles with distinctive electrophoretic mobilities. Segregation of 10 alternate alleles were observed in studies of F2 progeny: four for Av1, and two each for the other three loci. Additional variation found among the surveyed cultivars suggested at least two additional electrophoretically variant polypeptides. Several of the alleles were found to be associated with cultivars from specific geographic regions. Two examples were (i) the near exclusive association of the Av10.76 allele with Canadian cultivars and (ii) the high association of the Av10.58 allele with fall-planted cultivars. Fifty percent (SE ± 10.7%) of the fall-planted cultivars have the Av40.58 allele compared with 27.1% (SE ± 8.8%) of spring-planted cultivars.Key words: avenins, prolamines, polyacrylamide gel electrophoresis, linkage.

DISCRIMINATION OF CULTIVARS OF LENTIL (Lens culinaris Medic.) BY ELECTROPHORESIS OF SEED PROTEINS

1989 ◽  
Vol 69 (1) ◽  
pp. 243-246 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
K. W. CLARK
Keyword(s):  
Gel Electrophoresis ◽  
Lens Culinaris ◽  
Seed Protein ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel

Discrimination of lentil cultivars was achieved by analysis of seed protein by two types of polyacrylamide gel electrophoresis. Cultivars of lentil were discriminated by the presence or absence of diagnostic bands. Electrophoregrams of six seed lots of the cultivar Eston were identical and unaffected by growing conditions.Key words: Lens culinaris Medic, seed proteins, polyacrylamide gel electrophoresis, cultivar identification

scholarly journals Anomalous migration of leghaemoglobin on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis

1978 ◽  
Vol 169 (1) ◽  
pp. 251-253 ◽  
Author(s):  
P Lehtovaara
Keyword(s):  
Molecular Weight ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Relative Mobility ◽  
Concomitant Decrease ◽  
Helical Content

The estimate of the molecular weight of leghaemoglobin by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis is about 20% too low. This is due to an anomalously high limiting relative mobility. Leghaemoglobin binds 1.4 g of sodium dodecyl sulphate/g of protein with a concomitant decrease in the helical content from 71-72% to 49-51%.

scholarly journals Electrophoretic analysis, labeling and isolation of Chlamydomonas reinhardtii flagellum membrane proteins

2015 ◽  
Vol 48 (4) ◽  
pp. 511-521
Author(s):  
Aleksander F. Sikorski
Keyword(s):  
Membrane Proteins ◽  
Chlamydomonas Reinhardtii ◽  
Outer Surface ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Analysis ◽  
Polyacrylamide Gel ◽  
Electrophoretic Patterns

SDS-polyacrylamide electrophoretic patterns of <i>Chlamydomonas</i> flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and internal localization of fractions '4' and '6'. Application of SDS-polyacrylamide gel electrophoresis and radiolabeled membranes allowed to isolate individual membrane polypeptides.

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