PREDICTING NITROGEN AND DIGESTIBILITY OF FORAGES USING NEAR INFRARED REFLECTANCE PHOTOMETRY

1981 ◽  
Vol 61 (1) ◽  
pp. 45-51 ◽  
Author(s):  
J. E. WINCH ◽  
HELEN MAJOR

A Technicon Infra Alyzer 2.5, near infrared reflectance analyzer equipped with the six filters (1.68, 1.94, 2.10, 2.15, 2.23, 2.31 μm) which are used for the estimation of percent nitrogen, oil and moisture of grain crops was evaluated for the analyses of percent total nitrogen, in vitro and in vivo dry matter digestibility in grasses, legumes and grass-legume mixtures. Of the three quality parameters, only percent nitrogen was estimated with an acceptable degree of accuracy. The analyzer, therefore, has a potential for rapid nitrogen analysis of grasses, legumes and grass-legume mixtures. Correlation coefficients of 0.90 and above were obtained between infrared and Kjeldahl nitrogen when the nitrogen content of grasses or legume test samples was derived on either a grass or a legume calibration. A slight decrease in the standard error of prediction occurred when grass and legume calibrations were used to estimate nitrogen content of grass and legume samples, respectively. To insure accuracy, samples used to develop calibrations as well as those to be analyzed should be finely ground. In addition, the moisture content of the samples must be kept within the moisture range of the calibration.

1987 ◽  
Vol 67 (3) ◽  
pp. 747-754 ◽  
Author(s):  
E. V. VALDES ◽  
R. B. HUNTER ◽  
L. PINTER

The prediction of quality parameters by near infrared reflectance analysis (NIRA) in whole-plant corn (Zea mays L.) was studied. Quality parameters included percent protein and in vitro dry matter digestibility (IVDMD). Calibrations were developed using two types of NIRA instruments: (a) a Neotec 51A, a six-tilting-filters type instrument and (b) a Technicon InfraAlyzer 400R, a 19-fixed-filters type instrument. Plant samples were collected from four locations across Ontario (Brucefield, London, Guelph and Elora). Forty samples were used in the calibration sets (C) for each quality parameter and for each instrument. A second group of samples, a prediction set (P) consisting of 200 samples, was used to validate the calibration equations. Regression analysis between NIRA predicted and IVDMD, indicated that this parameter was well predicted in both C and P sets and with the two instruments. Coefficients of determination (r2) for C and P sets were 0.91 and 0.85 for the InfraAlyzer 400R and 0.92, and 0.81 for the Neotec 51A, respectively. A standard error of the estimate (SEE) of 1.70 was observed for the prediction of IVDMD in both C and P sets with the InfraAlyzer 400R. Values of SEE for IVDMD using the Neotec 51A were 1.76 and 1.73 for C and P sets, respectively. NIRA predictions of percent protein showed differences between instruments. The r2 for C and P sets were 0.95 and 0.81 for the InfraAlyzer 400R and 0.90 and 0.58 for the Neotec 51A, respectively. The low r2 value for percent protein in the P set might be related to the mathematical treatment of the reflectance data chosen for the analysis. The SEE for the prediction of percent protein varied between 0.25 and 0.56.Key words: Corn (whole-plant), quality, infrared reflectance


1987 ◽  
Vol 67 (2) ◽  
pp. 557-562 ◽  
Author(s):  
E. V. VALDES ◽  
R. B. HUNTER ◽  
G. E. JONES

A comparison of two near infrared (NIRA) calibrations (C1 and C2) for the prediction of in vitro dry matter digestibility (IVDM) in whole-plant corn (WPC) was conducted. C1 consisted of 40 WPC samples collected from four locations across Ontario (Brucefield, London, Guelph and Elora). C2 consisted of 90 samples and included the above locations plus Pakenham and Winchester. Nine wavelengths were used in both equations but only three were common in C1 and C2 equations. These wavelengths were 2139 nm, 2100 nm, and 1445 nm, respectively. The predictions of IVDM utilizing both C1 and C2 were good. Coefficients of determination (r2) and standard error of the estimate (SEE) for calibration and prediction sets were 0.91, 1.7; 0.85, 1.7 for C1 and 0.88, 1.6; 0.77, 1.6 for C2 respectively. Regression analysis within location, however, showed low r2 values for the prediction of IVDM for Pakenham and Winchester in both calibrations. The more mature stage of harvest at these locations might be the cause of the poorer predictions. Key words: In vitro digestibility, whole-plant corn, near infrared reflectance


1996 ◽  
Vol 1996 ◽  
pp. 226-226
Author(s):  
S.J. Lister ◽  
M.S. Dhanoa ◽  
J.L. Stewart ◽  
M. Gill

The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron bag technique, provides information relating to the fermentable fractions and kinetics of a forage and these parameters can be related to the NIR spectra. The aim of this study was to investigate the relationship between NIR spectra and cumulative gas production and derived parameters in tree leaves.


1996 ◽  
Vol 1996 ◽  
pp. 226-226
Author(s):  
S.J. Lister ◽  
M.S. Dhanoa ◽  
J.L. Stewart ◽  
M. Gill

The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron bag technique, provides information relating to the fermentable fractions and kinetics of a forage and these parameters can be related to the NIR spectra. The aim of this study was to investigate the relationship between NIR spectra and cumulative gas production and derived parameters in tree leaves.


2006 ◽  
Vol 86 (1) ◽  
pp. 157-159 ◽  
Author(s):  
G. C. Arganosa ◽  
T. D. Warkentin ◽  
V. J. Racz ◽  
S. Blade ◽  
C. Phillips ◽  
...  

A rapid, near-infrared spectroscopic method to predict the crude protein contents of 72 field pea lines grown in Saskatchewan, both whole seeds and ground samples, was established. Correlation coefficients between the laboratory and predicted values were 0.938 and 0.952 for whole seed and ground seed, respectively. Both methods developed are adequate to support our field pea breeding programme. Key words: Field pea, near-infrared reflectance spectroscopy, crude protein


1991 ◽  
Vol 31 (2) ◽  
pp. 205 ◽  
Author(s):  
KF Smith ◽  
PC Flinn

Near infrared reflectance (NIR) spectroscopy is a rapid and cost-effective method for the measurement of organic constituents of agricultural products. NIR is widely used to measure feed quality around the world and is gaining acceptance in Australia. This study describes the development of an NIR calibration to measure crude protein (CP), predicted in vivo dry matter digestibility (IVDMD) and neutral detergent fibre (NDF) in temperate pasture species grown in south-western Victoria. A subset of 116 samples was selected on the basis of spectral characteristics from 461 pasture samples grown in 1987-89. Several grass and legume species were present in the population. Stepwise multiple linear regression analysis was used on the 116 samples to develop calibration equations with standard errors of 0.8,2.3 and 2.2% for CP, NDF and IVDMD, respectively. When these equations were tested on 2 independent pasture populations, a significant bias existed between NIR and reference values for 2 constituents in each population, indicating that the calibration samples did not adequately represent the new populations for these constituents. The results also showed that the H statistic alone was inadequate as an indicator of equation performance. It was confirmed that it was possible to develop a broad-based calibration to measure accurately the nutritive value of closed populations of temperate pasture species. For the resulting equations to be used for analysis of other populations, however, they must be monitored by comparing reference and NIR analyses on a small number of samples to check for the presence of bias or a significant increase in unexplained error.


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