PATHOGENICITE COMPAREE D’ERWINIA CAROTOVORA VAR. ATROSEPTICA ET VAR. CAROTOVORA PAR INOCULATION DE CULTIVARS DE POMME DE TERRE

1979 ◽  
Vol 59 (2) ◽  
pp. 411-416 ◽  
Author(s):  
M. CARON ◽  
R. O. LACHANCE ◽  
G. PELLETIER
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
Potato Cultivars ◽  
Inoculation Methods ◽  
Pomme De Terre ◽  
Pathogenicity Tests

Three inoculation methods have been used to compare the pathogenicity of Erwinia carotovora var. atroseptica and var. carotovora on different potato cultivars: stem inoculation, inoculation of pregerminated seed pieces and inoculation of non-pregerminated seed pieces. Results could be obtained after only 5 days by stem inoculation. Stem inoculation by E. atroseptica has caused a black soft rot of the stem while E. carotovora caused a rather brown soft rot. A comparison between the two organisms was possible when pregerminated tuber pieces were used, which was not possible without pregermination. The inoculation of pregerminated seed pieces proved to be more adapted to all cultivars than stem inoculation for pathogenicity tests.

Assessing potato cultivars for resistance to tuber soft rot (Erwinia carotovora subsp.atroseptica) at four test centres in the UK

1988 ◽  
Vol 31 (1) ◽  
pp. 67-72 ◽  
Author(s):  
R. L. Wastie ◽  
G. J. Jellis ◽  
D. H. Lapwood ◽  
C. Logan ◽  
G. Little ◽  
...  
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
Potato Cultivars ◽  
Erwinia Carotovora Subsp.Atroseptica ◽  
The Uk ◽  
Soft Rot Erwinia

scholarly journals Erwinia carotovora pv. carotovora, causal agent of soft rot disease in some crops in Puerto Rico

1969 ◽  
Vol 74 (1) ◽  
pp. 83-92
Author(s):  
Amelia Cortés-Monllor
Keyword(s):  
Puerto Rico ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Causal Agent ◽  
Bacterial Isolates ◽  
Soft Rot Disease ◽  
Calla Lily ◽  
Pathogenicity Tests ◽  
Rot Disease ◽  
Antibiotic Response

The bacterium Erwinia carotovora pv. carotovora (Jones) was identified from bacterial isolates from decaying material of tobacco, tomato, tanier, head lettuce, cabbage, Dracaena spp, Pothos spp, and calla lily as the causal agent of soft rot disease. Physiological characteristics, biochemical reactions and antibiotic response were similar among strains. The ability of the isolates to produce soft rot symptoms on healthy plants when artificially inoculated was demonstrated by pathogenicity tests.

scholarly journals Identification and Pathogenicity of Phytopathogenic Bacteria Associated with Soft Rot Disease of Girasole Tuber

2012 ◽  
Vol 4 (1) ◽  
pp. 75-81
Author(s):  
Mamdoh Ewis ISMAIL ◽  
Montaser Fawzy ABDEL-MONAIM ◽  
Yasser Mahmoud MOSTAFA
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bacterial Isolates ◽  
Potato Tubers ◽  
Growing Seasons ◽  
Pathogenicity Tests ◽  
Rot Disease ◽  
Reducing Substances ◽  
Specific Symptoms ◽  
Deep Cavities

During 2010-2011 growing seasons six bacterial isolates were separated from naturally infected girasole plants tubers (Helianthus tuberosus L.) cv. ‘Balady’, showing soft rot, collected from experimental Farm of the Faculty of Agriculture, in El-Minia University, Egypt. Pathogenicity tests showed various virulence for the bacteria isolated from girasole tubers, found pathogenic. These organisms were characterized as rod-shaped, Gram negative, α-methyl-d-glucoside medium, reducing substances from sucrose, phos, phatase activity and deep cavities on pectate medium. Otherwise, diagnostic tests suggested that the pathogen was Erwinia carotovora ssp. carotovora. The isolated bacteria caused soft rot of wounded tubers when inoculated into tissues. The bacterial isolates were compared for their degree of pathogenicity as well as for differences in specific symptoms, induced in different hosts. The tested isolates could infect several host ranges, such as fruits of apricot, apple, olive, lemon, squash, eggplant and potato tubers, bulbs and garlic and onion cloves, roots radish, carrot, sweet potato and rape. On the other hand, no symptoms were exhibited on pods of bean and cowpea, faba bean, fruits of pepper and tomato. The extracts of experimentally diseased girasole tubers were active in pectinase and also in caboxymethyl cellulose at pH 6 compared to enzyme activities in healthy tissues. Also, the isolated bacteria increased the total and reducing sugars in infected tissues.

scholarly journals Evaluation of potato cultivars and breeding lines for susceptibility to tuber soft rot induced by Erwinia carotovora subsp atroseptica

2009 ◽  
Vol 62 ◽  
pp. 403-403
Author(s):  
M. Jeram ◽  
P.J. Wright ◽  
J.A.D. Anderson ◽  
S.L. Lewthwaite
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
Storage Conditions ◽  
Potato Cultivars ◽  
Control Measures ◽  
Postharvest Disease ◽  
Potato Tubers ◽  
Breeding Lines ◽  
Susceptibility To Infection ◽  
Potato Breeding Programme

Bacterial soft rot of potato tubers in storage is an economically important postharvest disease worldwide Disease control measures include reduction of tuber damage at harvest provision of suitable storage conditions and breeding of resistant cultivars Tubers of 18 potato cultivars and breeding lines were assessed for susceptibility to infection by Erwinia carotovora subsp atroseptica (Eca) over three maincrop seasons between 2003 and 2007 Susceptibility to Ecainduced soft rot was determined by measuring the weight of rotted tissue in anaerobically incubated whole tubers 7 days after inoculation with Eca Potato lines differed in susceptibility to soft rot by a factor of >37 (weight of rotted tissue) Cultivars Crop 28 Ilam Hardy and 30975 were highly susceptible and 065/27 Crop 15 and Crop 16 were least susceptible to Ecainduced soft rot As a consequence of these trials several of these lines are now being used in Plant Food Researchs potato breeding programme in order to develop cultivars with lower soft rot susceptibility than cultivars currently grown

scholarly journals 001 Establishment of Methods in Evaluating the Susceptibility of Chinese Cabbage (Brassica campestris spp.) to Soft Rot Disease by Erwinia

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 440-441
Author(s):  
Won Jun ◽  
Soo-Seong Lee ◽  
Jongkee Kim
Keyword(s):  
Chinese Cabbage ◽  
Large Scale ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Experimental Plant ◽  
Bacterial Soft Rot ◽  
Breeding Lines ◽  
Inoculation Methods ◽  
Soft Rot Disease ◽  
Rot Disease

Three inoculation methods, including cutting of a leaf, drenching, and point inoculation, were compared in an effort to screen the susceptibility of Chinese cabbage to soft rot disease caused by Erwinia carotovora subsp. carotovora. Three- to 4-week-old seedlings from 10 lines of cabbage with 16-h-old bacterial culture were routinely used. Inoculated seedlings were kept at 25.0 ± 10.0 °C for 48 h with saturated water vapor using a plastic tunnel in a greenhouse. Sixty-day-old mature plants were produced and inoculated in a greenhouse. Severity of symptoms, which were observed from percentage of plant infected was scored as from 1 to 9, representing resistant to susceptible, respectively. The correlation between seedlings and mature plants from ten lines was evaluated among the three different inoculation methods. Point inoculation gave the most significant correlation (r = 0.843, P < 0.05) between seedlings and mature plants. A good correlation was also observed between point inoculation of seedlings and drenching of mature plants (r = 0.609, P < 0.05). Cutting of a leaf of seedlings was also correlated with point inoculation of mature plants (r = 0.609, P < 0.05). This method provides the advantage of being able to keep the experimental plant alive, as only one leaf is detached. The point inoculation method is simple and relatively sensitive, so it could be used for large-scale screening for this bacterial soft rot disease. From three different evaluation assays, it was concluded that the breeding lines, C3-28, C3-29 from Cornell Univ. (Geneva, N.Y.) and the cultivar Kweonsim319 were relatively resistant to bacterial soft rot, while the Cornell breeding line CC-25 and the `Rang-no' cultivar were relatively susceptible.

Screening of Antagonistic Bacterium to Control Konjac Soft Rot

2013 ◽  
Vol 726-731 ◽  
pp. 4427-4430
Author(s):  
Guo Hua Chen
Keyword(s):  
Bacterial Strain ◽  
Bacterial Pathogen ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Field Trials ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Plate Method ◽  
Antagonistic Bacterium

Konjac soft rot is a bottleneck limiting konjac yield caused by bacterial strain of Erwinia carotovora var. carotovora. In order to control konjac soft rot, soil samples were collected, and each sample was spread on surface of a plate seeded with E. carotovora var. carotovora in advance. Strains expressing antagonistic activities were selected and then isolated with streak plate method. One bacterial strain (named Z10) was obtained from soil by this method. In field trials, strain Z10 still showed antagonistic effect against the bacterial pathogen.

scholarly journals Isolation, Characterization, and Identification of Biological Control Agent for Potato Soft Rot in Bangladesh

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
M. E. Ali ◽  
A. A. Khan ◽  
A. M. Akanda ◽  
Md. Kamal Uddin ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Control Agent ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Antagonistic Bacteria ◽  
And Storage

A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacteriumErwinia carotovorasubsp.carotovora(Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was testedin vitroagainst the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited thein vitrogrowth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genusBacillusand the isolate E-45 asLactobacillussp. The stronger antagonistic activity against Ecc P-138 was found in E-65in vitroscreening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5–62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.

scholarly journals Potato cultivars vary in susceptibility to blackleg (Erwinia carotovora subsp atroseptica)

2009 ◽  
Vol 62 ◽  
pp. 403-403
Author(s):  
P.J. Wright ◽  
J.A.D. Anderson
Keyword(s):  
New Zealand ◽  
Bacterial Growth ◽  
Field Experiments ◽  
Erwinia Carotovora ◽  
Potato Cultivars ◽  
Plant Food ◽  
Breeding Lines ◽  
Susceptibility To Infection ◽  
Food Research ◽  
Commercial Cultivars

Ten potato cultivars were tested for susceptibility to infection by Erwinia carotovora subsp atroseptica (Eca) the causal agent of blackleg over two maincrop seasons in 200506 and 200607 The pathogen was inoculated into seed tubers using toothpicks charged with undiluted bacterial growth Blackleg incidence was assessed 56 weeks after planting The field experiments clearly confirmed that potato cultivars vary significantly in susceptibility to blackleg Some New Zealand cultivars had a lower susceptibility to blackleg than current commercial cultivars The new Plant Food Research release Summer Delight in particular had an exceptional level of resistance and will be used as a parent in efforts to develop potato cultivars with lower blackleg susceptibility than current local commercial cultivars Routine screening of Plant Food Research breeding lines and cultivars will continue to be carried out over several seasons to confirm results presented here and to determine further blacklegresistant germplasm

scholarly journals Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Muhamad Salman Umar ◽  
Sudarjat Sudarjat ◽  
Luciana Djaya
Keyword(s):  
Biological Control ◽  
Potato Tuber ◽  
Endophytic Bacteria ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

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