scholarly journals Identification and Pathogenicity of Phytopathogenic Bacteria Associated with Soft Rot Disease of Girasole Tuber

2012 ◽  
Vol 4 (1) ◽  
pp. 75-81
Author(s):  
Mamdoh Ewis ISMAIL ◽  
Montaser Fawzy ABDEL-MONAIM ◽  
Yasser Mahmoud MOSTAFA
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bacterial Isolates ◽  
Potato Tubers ◽  
Growing Seasons ◽  
Pathogenicity Tests ◽  
Rot Disease ◽  
Reducing Substances ◽  
Specific Symptoms ◽  
Deep Cavities

During 2010-2011 growing seasons six bacterial isolates were separated from naturally infected girasole plants tubers (Helianthus tuberosus L.) cv. ‘Balady’, showing soft rot, collected from experimental Farm of the Faculty of Agriculture, in El-Minia University, Egypt. Pathogenicity tests showed various virulence for the bacteria isolated from girasole tubers, found pathogenic. These organisms were characterized as rod-shaped, Gram negative, α-methyl-d-glucoside medium, reducing substances from sucrose, phos, phatase activity and deep cavities on pectate medium. Otherwise, diagnostic tests suggested that the pathogen was Erwinia carotovora ssp. carotovora. The isolated bacteria caused soft rot of wounded tubers when inoculated into tissues. The bacterial isolates were compared for their degree of pathogenicity as well as for differences in specific symptoms, induced in different hosts. The tested isolates could infect several host ranges, such as fruits of apricot, apple, olive, lemon, squash, eggplant and potato tubers, bulbs and garlic and onion cloves, roots radish, carrot, sweet potato and rape. On the other hand, no symptoms were exhibited on pods of bean and cowpea, faba bean, fruits of pepper and tomato. The extracts of experimentally diseased girasole tubers were active in pectinase and also in caboxymethyl cellulose at pH 6 compared to enzyme activities in healthy tissues. Also, the isolated bacteria increased the total and reducing sugars in infected tissues.

scholarly journals Erwinia carotovora pv. carotovora, causal agent of soft rot disease in some crops in Puerto Rico

1969 ◽  
Vol 74 (1) ◽  
pp. 83-92
Author(s):  
Amelia Cortés-Monllor
Keyword(s):  
Puerto Rico ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Causal Agent ◽  
Bacterial Isolates ◽  
Soft Rot Disease ◽  
Calla Lily ◽  
Pathogenicity Tests ◽  
Rot Disease ◽  
Antibiotic Response

The bacterium Erwinia carotovora pv. carotovora (Jones) was identified from bacterial isolates from decaying material of tobacco, tomato, tanier, head lettuce, cabbage, Dracaena spp, Pothos spp, and calla lily as the causal agent of soft rot disease. Physiological characteristics, biochemical reactions and antibiotic response were similar among strains. The ability of the isolates to produce soft rot symptoms on healthy plants when artificially inoculated was demonstrated by pathogenicity tests.

scholarly journals Variability of aggressiveness and virulence of Erwinia carotovora subsp. carotovorum causing the soft rot on potato tubers in the western of Algeria

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
M’hamed Benada ◽  
Boualem Boumaaza ◽  
Sofiane Boudalia ◽  
Omar Khaladi ◽  
Bettache Guessas
Keyword(s):  
Erwinia Carotovora ◽  
Soft Rot ◽  
In Vitro Tests ◽  
Potato Storage ◽  
Bacterial Isolates ◽  
Potato Tubers ◽  
Potato Plants ◽  
Set Up ◽  
Cultivar Susceptibility

Soft rot symptoms were observed on potato plants in several potato cultivars in the western part of Algeria. A total of four strains of Erwinia are devided as follow: i) three strains of bacteria isolated from diseased tissues and soil, identified as Erwinia carotovora subsp carotovorum using conventional bacteriological and biochemical methods; and ii) one strain as Erwinia sp, not pathogens. In vitro tests, on tuber slices were set up to determine slices weight lost, which allows to find differences in cultivar susceptibility and isolate aggressiveness. Among the five cultivars, Laura was the most susceptible than the others tested cultivars. Moreover, it was found that MAI isolate was the most virulent than the other bacterial isolates. The results of this study should allow an optimization of the potato storage, after considering the susceptibility of a given cultivar to soft rot development and the aggressiveness.

scholarly journals Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Muhamad Salman Umar ◽  
Sudarjat Sudarjat ◽  
Luciana Djaya
Keyword(s):  
Biological Control ◽  
Potato Tuber ◽  
Endophytic Bacteria ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

scholarly journals Effect of some chemicals on incidence of poato soft rot disease in Bangladesh

2017 ◽  
Vol 52 (2) ◽  
pp. 135-140 ◽  
Author(s):  
MM Rahman ◽  
AA Khan ◽  
IH Mian ◽  
AM Akanda ◽  
MZ Alam
Keyword(s):  
Acetic Acid ◽  
Boric Acid ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Potato Tubers ◽  
Soft Rot Disease ◽  
Bleaching Powder ◽  
Carotovorum Subsp ◽  
Rot Disease

Bactericidal effect was investigated by chemicals against potato soft rot bacteria in vitro and in storage. The chemicals were acetic acid, boric acid, bleaching powder, lactic acid, calcium hydroxide, calcium chloride, potassium chloride and sodium hypo-chloride. Among eight  chemicals only three chemicals viz. acetic acid, boric acid and bleaching powder showed bactericidal activity against potato soft rot bacteria  Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora) P-138 in vitro. Based on the results of in vitro experiment three chemicals, acetic acid, boric acid and bleaching powder were used to control soft rot disease of potato in storage. Fresh potato tubers were dipped in 0.2% solution/suspensions of acetic acid, boric acid and bleaching powder for 30 min. Then soft rot bacteria Pectobacterium carotovorum subsp. carotovorum P-138 was inoculated on potato. Finally potatoes were stored for 22 weeks in net bags in sterilized condition. All the three chemicals significantly decreased the infection rate, loss in weight and increased percentage of disease reduction (PDR) of potato. Boric acid was the most effective in controlling the soft rot disease of potato in storage followed by acetic acid and bleaching powder. So these chemicals may be used for seed purpose storage of potato tubers for year round storage at farmer’s level.Bangladesh J. Sci. Ind. Res. 52(2), 135-140, 2017

Use of 1,4-naphthoquinones for control of Erwinia carotovora

2004 ◽  
Vol 50 (11) ◽  
pp. 951-956 ◽  
Author(s):  
Luis F.C Medina ◽  
Valter Stefani ◽  
Adriano Brandelli
Keyword(s):  
Antimicrobial Effect ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Sodium Ascorbate ◽  
Bacterial Suspension ◽  
Oxygen Species ◽  
Potato Tubers ◽  
Phytopathogenic Bacteria ◽  
Minimal Inhibitory Concentrations

The antimicrobial effect of 5 naphthoquinones was tested against the phytopathogenic bacteria Erwinia carotovora. Disk diffusion tests and determination of minimal inhibitory concentrations (MIC) indicate that the compound naphthazarin (NTZ) has the best antibacterial activity among the naphthoquinones tested. Studies on the mode of action indicate the effect of NTZ was bactericidal at 10 µg/mL. When cultivation was done in the presence of sodium ascorbate, the restoration of E. carotovora growth was observed with 3 µg/mL NTZ, but not when a 10 µg/mL dose was used. The incubation of NTZ with bacterial suspension of E. carotovora resulted in important changes in the absorption spectra of this naphthoquinone, indicating that a redox reaction takes place. These results may suggest that NTZ induces an increase of reactive oxygen species that are toxic to the cell. The compound NTZ was also effective in preventing E. carotovora growth on potato tubers, inhibiting the soft rot development at a concentration of 2 mg/mL.Key words: antimicrobial, naphthazarin, phytopathogen, potato, 5,8-dihydroxy-1,4-naphthoquinone.

Bacteriocin-like substance inhibits potato soft rot caused by Erwinia carotovora

2006 ◽  
Vol 52 (6) ◽  
pp. 533-539 ◽  
Author(s):  
Florencia Cladera-Olivera ◽  
Geruza R Caron ◽  
Amanda S Motta ◽  
André A Souto ◽  
Adriano Brandelli
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Bacillus Licheniformis ◽  
Membrane Lipids ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bactericidal Effect ◽  
Potato Tubers ◽  
Transmission Electron ◽  
Bacterial Surfaces

Soft rot is a major problem encountered in potatoes during postharvest storage. The soft rot bacterium Erwinia carotovora was inhibited by a novel bacteriocin-like substance (BLS) produced by Bacillus licheniformis P40. The BLS caused a bactericidal effect on E. carotovora cells at 30 µg mL–1. Transmission electron microscopy showed that BLS-treated cells presented wrinkled bacterial surfaces and shrinkage of the whole cell, indicating plasmolysis. Erwinia carotovora cells treated with BLS were analyzed by FTIR showing differences in the 1390 cm–1 and 1250–1220 cm–1 bands, corresponding to assignments of membrane lipids. BLS was effective in preventing E. carotovora spoilage on potato tubers, reducing the symptoms of soft rot at 240 µg mL–1 and higher concentrations. Soft rot development was completely blocked at 3.7 mg mL–1. This BLS showed potential to protect potato tubers during storage. Key words: bacteriocin, plant pathogen, potato, soft rot, storage.

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