LYSINE SCREENING IN BARLEY WITH A MODIFIED UDY DYE-BINDING METHOD

1975 ◽  
Vol 55 (3) ◽  
pp. 685-689 ◽  
Author(s):  
R. S. BHATTY ◽  
K. K. WU
Keyword(s):  
Amino Acid ◽  
Hordeum Vulgare ◽  
Correlation Coefficient ◽  
Initial Screening ◽  
Protein Determination ◽  
Amino Acid Analyzer ◽  
Dye Binding ◽  
Meal Protein ◽  
Barley Genotypes

A modified Udy method of protein determination and an amino acid analyzer method were compared for lysine screening in a number of lines and cultivars of barley (Hordeum vulgare and H. distichum). The lysine values obtained with the modified Udy procedure gave a correlation coefficient of + 0.96 with the analyzer lysine expressed as grams of lysine per 100 g meal, and a correlation coefficient of + 0.80 when the lysine values were expressed as grams of lysine per 100 g of meal protein. The modified Udy method was satisfactory for initial screening of lysine in barley genotypes.

PROTEIN LEVELS IN DEVELOPING AND MATURE PEA SEEDS

1975 ◽  
Vol 55 (1) ◽  
pp. 185-190 ◽  
Author(s):  
S. PANDEY ◽  
E. T. GRITTON
Keyword(s):  
Correlation Coefficient ◽  
Protein Concentration ◽  
Dry Weight ◽  
Protein Determination ◽  
Protein Levels ◽  
Dye Binding ◽  
Percent Protein ◽  
Kjeldahl Method ◽  
Pea Seeds ◽  
Pea Seed

Nine cultivars of peas (Pisum sativum L.) were grown in 1971 and 1972 to study the changes in percent crude protein levels during maturation. Percent protein was found to vary with years, cultivars, and stages of maturity. The year × cultivar, year × maturity, and cultivar × maturity interactions were also important. On a dry weight basis, pea protein concentration as determined by the Kjeldahl method showed a decrease with increasing pea seed maturity through the canning stage. The correlation coefficient between the Kjeldahl and Udy dye-binding methods of protein determination was 0.86 at the mature seed stage, and negligible at the canning stage. There was a highly significant correlation (r = 0.91) between protein concentration at the canning and mature stages of seeds (Kjeldahl method). The correlation coefficient between protein values obtained by the dye-binding method of Ashworth et al. and the Kjeldahl method was lower than between the Kjeldahl and Udy methods.

COMPARISON OF METHODS FOR LYSINE SCREENING IN BARLEY

1976 ◽  
Vol 56 (1) ◽  
pp. 25-30 ◽  
Author(s):  
D. E. LABERGE ◽  
R. TKACHUK ◽  
D. R. METCALFE
Keyword(s):  
Amino Acid ◽  
Soluble Protein ◽  
Binding Capacity ◽  
Water Soluble ◽  
Ground Sample ◽  
High Lysine ◽  
Water Soluble Protein ◽  
Amino Acid Analyzer ◽  
Dye Binding ◽  
Highly Correlated

Two more rapid colorimetric procedures for crude protein were compared with amino acid analyses of lysine on an amino acid analyzer. The methods were found to be useful and inexpensive compared with amino acid analyses for segregating high-lysine selections of barley (Hordeum sp.). UDY absorbance, which is related to the dye-binding capacity of basic amino acids, was highly correlated with lysine when expressed as milligrams of lysine per 100 mg of ground sample (r = −0.919). When lysine was expressed as grams of lysine per 100 g of protein, the correlation coefficient was −0.479. Low UDY absorbance (i.e., high dye-binding capacity) can be obtained from higher lysine level or higher protein content. Therefore, for more accurate analyses, a Kjeldahl determination should be performed on the samples. A highly significant correlation (r = 0.753) was observed for ninhydrin absorbance of aqueous extracts when compared with lysine analyses. Application of this method is based on the assumption that high-lysine proteins are in the water-soluble protein fractions. Since high ninhydrin absorbance may be produced by increased soluble protein containing normal levels of lysine, the lysine content of samples screened by this method should be verified by amino acid analyses.

scholarly journals Determination of Methylated Basic Amino Acids with the Amino Acid Analyzer

1973 ◽  
Vol 248 (7) ◽  
pp. 2387-2391 ◽  
Author(s):  
Gladys E. Deibler ◽  
Russell E. Martenson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Basic Amino Acids ◽  
Amino Acid Analyzer

An improved amino acid analyzer

1964 ◽  
Vol 7 (2) ◽  
pp. 168-177 ◽  
Author(s):  
A.S. Inglis
Keyword(s):  
Amino Acid ◽  
Amino Acid Analyzer

scholarly journals Evaluation of Amino Acid and Vitamin B2 and E Composition of Genetically Modified Antidwarf Mosaic Maize by Automatic Amino acid Analyzer and HPLC

2013 ◽  
Vol 25 (6) ◽  
pp. 3525-3526 ◽  
Author(s):  
Pingmei Yan ◽  
Qing Wang ◽  
Xiaoyan Yan ◽  
Xiao-Hui Chang ◽  
Jian-Zhong Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetically Modified ◽  
Vitamin B2 ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

scholarly journals Evaluation of Different Flax Cultivars in Their Oil, Fatty Acids Protein and Amino Acids and Correlations

2017 ◽  
Vol 27 (1) ◽  
pp. 51-58
Author(s):  
Ahmed Abdullah Bakhashwain Ahmed Abdullah Bakhashwain
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Amino Acid ◽  
Linolenic Acid ◽  
Correlation Coefficients ◽  
Omega 3 ◽  
Geographical Regions ◽  
Meal Protein ◽  
Omega 6 ◽  
King Abdulaziz University

This study was carried out in the Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University to evaluate 7 flax genotypes from different geographical regions concerning their composition from oil and protein contents, fatty acids and amino acids, besides, calculate the correlation coefficients between the fatty acids and also between the amino acids.The main results showed that Verum cv. had the highest oil content and linolenic acid (Omega-3) while Hiera cv. was the highest in meal protein content and most amino acids. The highest amino acid concentration was proline and it ranged from 27.15% to 30.21%. Linolenic acid (Omega-3) negatively and significantly correlated with Oleic and Linoleic (Omega-6) fatty acids. Lysine amino acid positively and significantly correlated with the amino acids of Glutamine, Aspartic, Leucine, Serine and Isoleucine.

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