VARIATION OF FRACTION 1 PROTEIN CONTENT IN ALFALFA

1970 ◽  
Vol 50 (5) ◽  
pp. 537-539 ◽  
Author(s):  
D. H. HEINRICHS ◽  
J. E. MILTIMORE
Keyword(s):  
Medicago Sativa ◽  
Protein Content ◽  
Chemical Constituents ◽  
Fraction 1 Protein ◽  
Medicago Sativa L

An alfalfa population consisting of plants of Medicago sativa L., M. media Pers., and M. falcata L. varied significantly for Fraction 1 (18S) protein content. M. falcata plants, as a group, contained about half as much Fraction 1 protein as M. sativa plants while M. media plants tended to fall between these two in Fraction 1 protein content. It is suggested that M. falcata may be a source of low Fraction 1 protein content. There was no appreciable association between Fraction 1 protein content and other chemical constituents.

Shearing force of alfalfa stems: Effects of genotype and year interactions

1998 ◽  
Vol 78 (4) ◽  
pp. 719-722
Author(s):  
A. D. Iwaasa ◽  
K. A. Beauchemin ◽  
S. N. Acharya ◽  
J. G. Buchanan-Smith
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Key Words ◽  
Chemical Constituents ◽  
Stem Segment ◽  
Shearing Force ◽  
Medicago Sativa L ◽  
Genotype Clustering ◽  
Force Characteristics

Shearing force of alfalfa (Medicago sativa L.) stems was measured to evaluate genotype-by-year interactions. Based on mean shearing force for each stem segment and genotype clustering criteria, several genotypes had similarly shearing forces among years. Therefore, selecting genotypes with desirable shearing force characteristics to improve digestibility or intake potential of forages may be possible. Key words: Genotype, environment, shearing force, cell wall chemical constituents

scholarly journals The nutritional quality of feedstuffs used in dairy goat nutrition in Vojvodina

2019 ◽  
Vol 35 (2) ◽  
pp. 163-178
Author(s):  
Snezana Paskas ◽  
Jelena Miocinovic ◽  
Branislav Vejnovic ◽  
Zsolt Becskei
Keyword(s):  
Chemical Composition ◽  
Medicago Sativa ◽  
Protein Content ◽  
Nutritional Quality ◽  
Lignin Content ◽  
Dairy Goat ◽  
Crude Fibre ◽  
Significant Difference ◽  
Medicago Sativa L

The study was to conduct to evaluate the chemical composition and nutritive values of feedstuffs (forages and concentrate mixtures) used for dairy goats nutrition in Vojvodina. Samples were collected from six farms, including one organic farm. The results showed that the relative feed values of analyzed forages were in the range of good, medium to lower quality. Average protein content from lowest to highest for investigated forages was: corn silage (Zea Mays) (65.37-82.57g kg??DM), alfalfa haylage (Medicago sativa L.) (159.99-184.17g kg??DM), pasture (185.30g kg?? DM), and alfalfa hay (Medicago sativa L.) (167.48-203.60g kg??DM). The non-fibre carbohydrates and protein content most varied in organic hay samples (cv: 29.25% and 19.09%, respectively). Generally, feedstuffs used in organic nutrition, including organic concentrate, were of lower nutritional quality and moreover contained higher amounts of crude fibre and lignin. Especially, a high source of variation was observed in investigated concentrate mixtures for the crude protein content (p<0.0001), ranged from 135.32 to 209.87g kg??DM. Corn silages also varied substantially in their chemical composition and significant difference (p<0.05) was observed in regard to acid detergent fibre (ADF) and lignin content (ranged: ADF: 242.20-319.24g kg??DM; ADL: 27.98-52.54g kg??DM, respectively). Furthermore, pasture contained the most soluble materials during May and June and their content was related inversely to crude fibre amount. This survey highlights that investigated farms still pay insufficient attention to the quality of the feedstuff. For the development of intensive goat farming, greater emphasis should be placed on using higher quality feedstuffs, as well, standards for feed quality must be considered and established.

Water Requirement and Its Association With Growth Components and Protein Content of Alfalfa ( Medicago sativa L.) 1

Crop Science ◽  
1970 ◽  
Vol 10 (3) ◽  
pp. 237-240 ◽  
Author(s):  
D. F. Cole ◽  
A. K. Dobrenz ◽  
M. A. Massengale ◽  
L. Neal Wright
Keyword(s):  
Medicago Sativa ◽  
Protein Content ◽  
Water Requirement ◽  
Medicago Sativa L

Bloat in cattle fed alfalfa selected for a low initial rate of digestion

1994 ◽  
Vol 74 (3) ◽  
pp. 451-456 ◽  
Author(s):  
J. W. Hall ◽  
W. Majak ◽  
D. G. Stout ◽  
K.-J. Cheng ◽  
B. P. Goplen ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Protein Content ◽  
Key Words ◽  
Dry Matter ◽  
Initial Rate ◽  
Legume Species ◽  
The Third ◽  
Fourth Cycle ◽  
Medicago Sativa L

Investigations of legume species which do and do not cause bloat suggested that a bloat-safe alfalfa (Medicago sativa L.) might be produced by reducing its initial rate of digestion. This study compared a third cycle alfalfa synthetic selected for a low initial rate of digestion (LIRD) with a control cultivar (Beaver). The occurrence of bloat was not significantly lower when cattle were fed cycle 3 LIRD than when fed Beaver in three grazing trials or in two of three feedlot trials. The deviation of the results in the third trial could not be explained by differences in dry matter or protein content. When grown under irrigation, the 4 h dry matter disappearance (DMD) of cycle 3 LIRD was 89% to 96% of that for Beaver. This reduction was much less than has been hypothesized to prevent bloat. Without irrigation the 4 h DMD of both cultivars were reduced by 1–3% while cycle 3 LIRD continued to have a lower DMD than Beaver. LIRD may provide some protection against bloat on dry rangelands though the response may be variable in years when moisture is well supplied. Studies with a fourth cycle LIRD selection will investigate this further. Key words: Legumes, Medicago sativa L., nylon bag, digestion

DURÉE D’INTERFÉRENCE DU CHIENDENT, Agropyron repens (L.) Beauv. DANS LA LUZERNE, Medicago sativa L.

1987 ◽  
Vol 67 (2) ◽  
pp. 451-456
Author(s):  
D. PAGEAU ◽  
G. D. LEROUX
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Protein Content ◽  
Forage Quality ◽  
Medicago Sativa L ◽  
Agropyron Repens

A study was conducted at St-Augustin, Quebec, in 1984 and repeated in 1985 to determine the tolerable period of interference between alfalfa (Medicago sativa L.) and quackgrass (Agropyron repens (L.) Beauv.) during the year of establishment. Glyphosate was used prior to seeding of alfalfa to control quackgrass. Haloxyfop-methyl, a selective grass herbicide, was sprayed at different periods after crop emergence to control quackgrass. This herbicide was applied at 3, 6 and 9 wk after alfalfa emergence. A 3-wk period of interference provided equivalent proportion of alfalfa in forage and the same level of forage quality as the quackgrass-free control. Delaying haloxyfop-methyl treatments to more than 3 wk resulted in an increased proportion of quackgrass in forage which caused a reduction of the protein content and an increase of the cell wall contents (NDF and ADF). Alfalfa density did not differ between 0 and 3 wk of interference of quackgrass, but tended to be reduced with a longer period of interference.Key words: Quackgrass, alfalfa, duration of interference, haloxyfop-methyl, glyphosate, forage quality

scholarly journals CONTENT OF TOTAL NITROGEN AND PROTEINS FROM ALFALFA (Medicago sativa L.) COLLECTED IN THREE SLOPES

2019 ◽  
Vol 34 (3) ◽  
pp. 635-640
Author(s):  
Valentina Butleska Gjoroska ◽  
Liljana Koleva Gudeva ◽  
Lenka Cvetanovska
Keyword(s):  
Medicago Sativa ◽  
Protein Content ◽  
Plant Cell ◽  
Total Nitrogen ◽  
Nitrogen Concentration ◽  
Protein Composition ◽  
Forage Crops ◽  
Protein Nitrogen ◽  
Forage Crop ◽  
Medicago Sativa L

Alfalfa (Medicago sativa L.) leaves and stems contain different proteins and nitrogen concentration in different stages of growth. The objective of this study is to determine the dynamic of nutrient accumulation of total nitrogen and proteins in leaves and stems. The experiment was conducted in three slopes, on three regions in the Republic of North Macedonia (Tetovo, Skopje and Ovche Pole). Chemical analysis of total nitrogen and proteins were obtained from first, second and third slope. Modern techniques have been used for analyzing the protein activity of plant material in multiple measuring points. Significant differences are found in the production of total nitrogen and proteins between the locations in Tetovo region on one side, and Skopje and Ovche Pole on the other side. It shows that Tetovo region has better conditions for producing alfalfa protein. Alfalfa is a culture that is rich in high nitrogen and protein content in the Tetovo region, which is correlated with the amino acid composition, resulting in a high biological value. Therefore, alfalfa is the dominant forage crop and active diet culture with high applicability to the bio-diet. Proteins are the most abundant biomolecules in plants and other organisms. Protein macromolecules make up half of the dry matter in the plant cell. The plant cell contains many different proteins with a specific function. Proteins contain the most important property - biological specificity, so the individuality of each organism is conditioned by the type of protein it is made of. Proteins have a specific structure that is found in their biological activity. Proteins are the most important components in the plant cell. Nitrogen is one of the many compounds important for plant life processes and its role in physiological processes in plants is quite large. The needs of certain plants for nitrogen are different. Nitrogen in plants is important in the composition of proteins, nucleic acids, coenzymes, alkaloids, some pigments and other compounds. Accordingly, the nitrogen in plants exists as non-protein and protein nitrogen, found in the protein component. It can only enter the plant cell if it is reduced to ammonia. This scientific research paper provides a comprehensive analysis of the nitrogen and protein composition of alfalfa grown in the Skopje, Tetovo and Ovche Pole region. The results of this research, represent the first full and complete overview of alfalfa (Medicago sativa L.), with its protein composition, which would be of great importance for the further cultivation of this forage crop. Scientific evidence has shown that the Tetovo region has a higher advantage over the Skopje and Ovche Pole region in terms of nitrogen and protein content, which are crucial nutrients in forage crops.

Large-scale isolation of fraction 1 leaf protein (18S) from lucerne (Medicago sativa L)

1976 ◽  
Vol 86 (3) ◽  
pp. 495-501 ◽  
Author(s):  
W. T. Jones ◽  
J. L. Mangan
Keyword(s):  
Molecular Weight ◽  
Medicago Sativa ◽  
Ammonium Sulphate ◽  
Large Scale ◽  
Gel Filtration ◽  
Low Molecular Weight ◽  
Fraction 1 Protein ◽  
Ammonium Sulphate Fractionation ◽  
Medicago Sativa L ◽  
Soluble State

SummaryFraction 1 protein (18S) can be isolated in large quantities (order 100 g) in a soluble state by heating lucerne juice, adjusted to pH 6·7 to 6·9, from a Pirie extractor to 63 °C for 10 min. Low speed oentrifugation (2500 g) removed coagulated chloroplast fragments and most of the heat-denatured Fraction 2 proteins. Fraction 1 (18S) protein (> 95% pure) was purified from low molecular weight materials (sugars, phenolics etc.) by ammonium sulphate fractionation and gel filtration on Sephadex G-75 gels.

INFLUENCE DES CULTIVARS, DES DATES ET DES DOSES DE SEMIS SUR LE RENDEMENT ET LA QUALITÉ DE LA LUZERNE L’ANNÉE DU SEMIS

1984 ◽  
Vol 64 (2) ◽  
pp. 309-317
Author(s):  
L. BELZILE ◽  
R. RIOUX
Keyword(s):  
Medicago Sativa ◽  
Protein Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Total Yield ◽  
Crude Protein Content ◽  
Dry Matter Digestibility ◽  
Yield And Quality ◽  
Medicago Sativa L ◽  
Made In

The effects of cultivars, seeding rates and dates on alfalfa (Medicago sativa L.) yield and quality in the seeding year were studied from 1971 to 1975 on a Kamouraska clay. Results showed the importance of seeding alfalfa before the end of May. Seedings made in early and in mid-May resulted in equivalent yield. Differences between cultivars were more important on the second than on the first cut. Regrowth was an important factor in the seedling year. The total yield of the Alfa cultivar is higher than Vernal and Narragansett and it is also higher for every combination of rates and seeding dates. Seeding rates were not an important factor except at the first cut where a rate of 6.7 kg/ha gave a lower yield than a rate of 13.4 kg/ha. Dry matter digestibility of Alfa is sometimes higher than for the other cultivars in the study. Late seeding generally increased the digestibility of the second cut dry matter, whereas rates of seeding have no effect on either first or second cut material. The crude protein content is not affected by seeding rates and the effects of cultivars are generally very small. With a delay in seeding dates the crude protein content of the second cut material is sometimes increased.Key words: Medicago sativa L., cultivar, date, rate

Change of protein content in plants of Medicago sativa L. under the influence of soil contamination with oil and reclamation with the application of the preparation "Elena"

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 324-328
Author(s):  
Yu.M. Sotnikova ◽  
A.S. Grigoriadi ◽  
R.G. Farkhutdinov
Keyword(s):  
Medicago Sativa ◽  
Protein Content ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Oil Pollution ◽  
Higher Plants ◽  
Practical Interest ◽  
Morphometric Parameters ◽  
Biological Product ◽  
Medicago Sativa L

The study of the adaptive mechanisms of plants in response to the action of oil makes it possible to identify signs that ensure the resistance of plants in conditions of oil pollution, with the aim of using them in the development of methods of reclamation and selection of plants resistant to oil pollution for it. The available data on the effects of petroleum hydrocarbons on plants are highly controversial. The species diversity of plants capable of growing on oil-contaminated soils has not been sufficiently studied. Phytoremediation is the most suitable soil purification tool for agricultural areas due to the large extent of the land and the relative cheapness of the method. Physiological, biochemical and morphometric parameters are important indicators of plant resistance to hydrocarbon pollutants. Of great scientific and practical interest is the identification of the resistance of higher plants to exogenous petroleum hydrocarbons (HC) and the identification of the effect of phytoremediation and the effect of the introduction of biological products in case of hydrocarbon pollution of the soil. The aim of the work was to assess changes in the biochemical and morphometric parameters of Medicago sativa L. plants under conditions of soil pollution with oil and reclamation using the biological product "Elena". Determination of the protein content in leaves makes it possible to judge the status of nitrogen metabolism in plants. It was revealed that oil pollution led to a decrease in the protein content of the plant Medicago sativa L. The use of the biological product "Elena" led to the restoration of this parameter. The results showed that the positive effect of the studied biological product depends on the concentration of the pollutant.

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