VARIETAL DIFFERENCES IN CALCIUM LEVEL IN LEAVES OF FLUE-CURED TOBACCO

1963 ◽  
Vol 43 (2) ◽  
pp. 141-145 ◽  
Author(s):  
E. T. McEvoy

Radioactivity measurement of leaf samples of tobacco plants following a 6-day absorption period in Ca45-labelled nutrient solution was found to be a rapid test for varietal differences in calcium uptake. Using this method, significant differences in the uptake of calcium were found among varieties and strains of flue-cured tobacco and the ranking of the calcium uptake potential of five strains of tobacco agreed with that obtained by chemical assay. The concentration of Ca45 in the leaf tissue was highest in Delcrest and Hicks, lowest in White Mammoth, and intermediate in Virginia Gold, White Gold, and Jadel. The same ranking of these varieties was obtained when the plants were grown in soil with added Ca45 for a 7-week absorption period. The importance of these tests is based on the findings of other workers that leaf content of calcium is inversely related to quality.

1964 ◽  
Vol 44 (3) ◽  
pp. 263-264 ◽  
Author(s):  
E. T. McEvoy

Six varieties of flue-cured tobacco were tested in the greenhouse in soil for differences in phosphorus level in the leaves, using radiophosphorus. Radioactivity measurement of leaf samples showed significant varietal differences in P32 activity in the leaves. The level of P32 per sq. cm of leaf tissue was higher in Delcrest, Hicks, and Virginia Gold than in White Gold, Jadel, and White Mammoth.


1984 ◽  
Vol 24 (125) ◽  
pp. 213 ◽  
Author(s):  
GCJ Irving ◽  
D Bouma

Experiments were done to determine what proportion of the phosphate extracted from fresh leaf tissue by five drops of 10 N H2SO4 represents inorganic tissue phosphate, and to what extent hydrolysis of organic phosphates during and after the extraction, and during the development of the blue phosphomolybdate complex, could contribute to the values obtained. The extraction is the basis of a simple and rapid test for the assessment of the phosphorus status of subterranean clover (Bouma and Dowling 1982). Extraction of leaf tissue of subterranean clover and sunflower with 0.2 M HClO4 at O�C, which was shown to extract inorganic leaf phosphorus without causing significant hydrolysis of organic phosphates, gave values not significantly different from those in H2SO4 extracts. The rate of hydrolysis of endogenous organic phosphates in tissue, extracted and left at room temperature for periods of up to 40 min. after adding H2SO4, did not differ significantly from zero. Errors due to hydrolysis during the 30 min. previously recommended for colour development are reduced to negligible proportions by reducing the time for colour development to 10 min. and by adding citric acid at this point. Anion-exchange chromatography of 10 N H2SO4 and 0.2 M HClO4 extracts confirmed the similarity of their composition and provided estimates of the various phosphate compounds present. The extraction of fresh leaf tissue with 10 N H2SO4 provides a satisfactory estimate of the endogenous inorganic phosphorus content.


1985 ◽  
Vol 40 (7-8) ◽  
pp. 571-575 ◽  
Author(s):  
Wilhelm Hasselbach ◽  
Andrea Migala

Abstract The decline of the transport ratio of the sarcoplasmic calcium pump observed in a recent study (A. results from the retardation of calcium oxalate precipitation at low calcium/protein ratios. The prevailing high internal calcium level supports a rapid calcium backflux and a compensatory ATP hydrolysis during net calcium uptake which reduces the transport ratio. Yet, the determined calcium back­ flux does not fully account for the decline of the transport ratio. A supposed modulation of the stoichiometry of the pump by external calcium (0.1 μм) is at variance with results of previous studies showing a constant transport ratio of two in the same calcium concentration range.


1967 ◽  
Vol 20 (3) ◽  
pp. 575 ◽  
Author(s):  
CB Osmond

The leaves of AtripZe:c and eight related genera of the Chenopodiaceae from semi-arid Australia oontained high levels of oxalate. Most of the oxalate was in the soluble form and was correlated with high cation content generally rather than with calcium level. In water-culture experiments calcium absorption and oxalate synthesis varied independently.


Author(s):  
Dalva Paulus ◽  
Ezequiel Toffoli ◽  
Celso Eduardo Pereira Ramos ◽  
Claúdia de Andrade Moura ◽  
Dislaine Becker ◽  
...  

Aims: The reuse of fish wastewater in agricultural activities such as the production of seedlings in commercial nurseries has great potential to minimize production costs and to reduce environmental impacts due to the inappropriate disposal of this waste. The objective of this study was to evaluate the growth, development and quality of Eucalyptus grandis W. Hill ex Maiden seedlings produced with different wastewater concentrations from fish farming. Study Design:  The fertigation treatments were using fish farming (Tilapia) wastewater from tanks (FW), daily nutrient solution (DNS), and the combinations of 50% FW + 50% DNS, and 25% DNS + 75% FW, applied daily. Place and Duration of Study: The experiment was carried out from August to December 2016 at the Federal University of Technology of Paraná, Brazil. Methodology: The macro and micronutrient contents in leaf tissue, seedling height, stem diameter, the largest root length, leaf area, fresh and dry shoot and root mass and Dickson quality index were all evaluated. Results: The nutrient contents present in the leaf tissue were adequate for the nutritional demand of the eucalyptus. The seedling quality index (DQI) indicated that the daily nutrient solution (0.21) and 50% FW + 50% DNS (0.20) generated balanced seedlings regarding height and biomass accumulation. Conclusion: Fertigation of eucalyptus seedlings can be carried out with wastewater from fish farming, but it is necessary to complement the fertilization with other nutrient sources to produce quality seedlings which are suitable for field transplantation.


HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 622-627 ◽  
Author(s):  
Guiseppe Colla ◽  
Youssef Roupahel ◽  
Mariateresa Cardarelli ◽  
Elvira Rea

A greenhouse experiment was carried out to determine growth, yield, fruit quality, gas exchange and mineral composition of watermelon plants (Citrullus Lanatus L. `Tex'), either ungrafted or grafted onto two commercial rootstocks `Macis' [Lagenaria siceraria (Mol.) Standl.] and `Ercole' (Cucurbita maxima Duchesne × Cucurbita moschata Duchesne) and cultured in NFT. Plants were supplied with a nutrient solution having an electrical conductivity (EC) of 2.0 or 5.2 dS·m–1. The saline nutrient solution had the same basic composition, plus an additional of 29 mm of NaCl. Increased salinity in the nutrient solution decreased total yield. The reduction in total yield in saline treatments compared to control was due to a reduction in the fruit mean mass and not to the number of fruit per plant. Total fruit yield was 81% higher in grafted than in ungrafted plants. The lowest marketable yield recorded on ungrafted plants was associated with a reduction in both fruit mean mass and the number of fruits per plant in comparison to grafted plants. Salinity improved fruit quality in all grafting combinations by increasing dry matter (DM), glucose, fructose, sucrose, and total soluble solid (TSS) content. Nutritional qualities of grafted watermelons such as fruit DM, glucose, fructose, sucrose, and TSS content were similar in comparison to those of ungrafted plant. In all grafting combinations, negative correlations were recorded between Na+ and Cl– in the leaf tissue and net assimilation of CO2 Grafting reduced concentrations of sodium, but not chloride, in leaves. However, the sensitivity to salinity was similar between grafted and ungrafted plants and the higher total yield from grafting plants was mainly due to grafting per se.


1969 ◽  
Vol 47 (10) ◽  
pp. 1595-1598 ◽  
Author(s):  
T. T. Lee ◽  
N. Rosa

Gibberellic acid (GA3) significantly reduced the level of starch in green leaves of tobacco plants. The total α-amylase activity and the specific activities of α-amylase and invertase were increased by GA3 treatment. Hydrolysis of starch during flue-curing contributed greatly to the increased level of reducing sugars in cured leaf tissue; decreasing the level of starch in green leaves by GA3 effectively lowered the level of reducing sugars after curing.


2022 ◽  
Vol 7 (1) ◽  
pp. 41-49
Author(s):  
Ľuboš Vrtoch ◽  
Martin Pipíška ◽  
Miroslav Horník ◽  
Jozef Augustín

Tobacco has previously been used in investigations of metals and radionuclide uptake. This study presents determination of bioaccumulation and translocation of 60Co2+ ions in tobacco plants (Nicotiana tabacum L.) grown in Hoagland’s nutrient solution. Cobalt concentration in tobacco plants increased with increasing concentration in nutrient solution. Bioaccumulation from the initial concentration C0 = 0.96 μM Co reached 90% after 7 day cultivation. Only small amounts of Co accumulated in roots, up to 2 - 4 % were removable from roots by washing with 0.1 M CoCl2, indicating that this portion of Co is bound to the root surface in ion-exchangeable form. Tobacco roots retained approximately 2/3 of accumulated cobalt and 1/3 was transported to shoots. Autoradiography revealed that 60Co was preferentially localized in younger leaves. Prolongation of cultivation time did not change the [Co]roots : [Co]shoots ratio significantly. Relationships between growth rate, transpiration rate, uptake and distribution of cobalt in plant tissue are discussed.


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