Screening sweet cherry cultivars from the Pacific Agri-Food Research Centre Summerland breeding program for resistance to brown rot (Monilinia fructicola)

2008 ◽  
Vol 88 (4) ◽  
pp. 747-752 ◽  
Author(s):  
Frank Kappeland ◽  
Peter L Sholberg
Keyword(s):  
Prunus Avium ◽  
Brown Rot ◽  
Breeding Program ◽  
Research Centre ◽  
Monilinia Fructicola ◽  
Conidial Suspension ◽  
Food Research ◽  
The Pacific ◽  
Sweet Cherries ◽  
Sweet Cherry Cultivars

Recently released cultivars and advanced selections of sweet cherries (Prunus avium L.) from the breeding program at the Pacific Agri-Food Research Centre at Summerland, British Columbia, were screened for resistance to brown rot [Monilinia fructicola (Wint.)]. From 2000 to 2003 a range of sweet cherries were inoculated by spraying with a conidial suspension of 1 × 104 colony-forming units per mL of Monilinia fructicola, placed in a growth chamber at 13°C and rated for rot at 8 and 11 d after inoculation. The response of cultivars or selections varied from year to year. In 2000, 10 of 14 cultivars had disease incidences of over 50% by day 8, whereas, in 2002, only 2 of 19 cultivars had disease incidences greater than 50%. Staccato™, Stardust and Sweetheart were in the least susceptible category, that is, the percent rot infection was significantly less than the mean of all genotypes in 2 of the 4 yr. Santina, Skeena, and 13N-07-32 were in the least susceptible category in 1 of 4 yr. The level of resistance to brown rot was not very high. For the least susceptible cultivars such as Staccato™, Stardust, or Sweetheart, the level of resistance was not high enough to grow the crop without fungicide applications. Key words: Prunus avium, resistance, inoculation

SPC103 (Sentennial™) sweet cherry

2011 ◽  
Vol 91 (1) ◽  
pp. 179-181 ◽  
Author(s):  
Frank Kappel ◽  
Richard MacDonald ◽  
Rob Brownlee ◽  
Darrell-Lee McKenzie
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Fruit Size ◽  
Breeding Program ◽  
Research Centre ◽  
Food Research ◽  
The Pacific ◽  
Commercial Testing ◽  
Prunus Avium L

Kappel, F., MacDonald, R. A., Brownlee, R. T. and McKenzie, D.-L. 2011 SPC103 (Sentennial™) sweet cherry. Can. J. Plant Sci. 91: 179–181. SPC103 (Sentennial™) is a sweet cherry (Prunus avium L.) that has been released by the Pacific Agri-Food Research Centre (PARC-Summerland), Agriculture and Agri-Food Canada at Summerland, BC, as part of the ongoing sweet cherry breeding program that began in 1936. Sentennial™ is the latest maturing cultivar released from PARC-Summerland and has very good fruit size and firmness. It was released to growers for advanced commercial testing in 2006. The name Sentennial™ was chosen in honour of the District of Summerland's centennial year in 2006 and it was spelled with an “S” to maintain the tradition of starting most cherry cultivar names from the Summerland breeding program with an “S”.

13N0770 (Stardust™) sweet cherry

2009 ◽  
Vol 89 (4) ◽  
pp. 713-716 ◽  
Author(s):  
F Kappel ◽  
R A MacDonald ◽  
M Cliff ◽  
D -L Mckenzie
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Fruit Size ◽  
Research Centre ◽  
Fruit Breeding ◽  
Food Research ◽  
The Pacific ◽  
Cultivar Description ◽  
Wide Scale ◽  
Commercial Testing

13N0770 (Stardust™) is a late-maturing blush sweet cherry (Prunus avium L.) that has been released by the Pacific Agri-Food Research Centre (PARC-Summerland), Agriculture and Agri-Food Canada at Summerland, BC. The release is part of the on-going sweet cherry breeding program that began in 1936 (Kappel and Lay 1997). Stardust™ is the latest-maturing blush or bi-colored cultivar released from PARC-Summerland and has very good fruit size, firmness, and is self-fertile. It was released to growers for wide scale commercial testing in 2002.Key words: Prunus avium, fruit breeding, cultivar description

scholarly journals First Report of Brown Rot Caused by Monilinia fructicola in Sweet Cherry in Maryland

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 145-145 ◽  
Author(s):  
F. Chen ◽  
X. Liu ◽  
G. Schnabel
Keyword(s):  
Sweet Cherry ◽  
Prunus Avium ◽  
Brown Rot ◽  
Causal Agent ◽  
Monilinia Fructicola ◽  
Conidial Suspension ◽  
Sterile Water ◽  
Tomato Sauce ◽  
Control Fruit ◽  
Petri Dishes

Monilinia fructicola (G. Wint.) Honey is the most important causal agent of brown rot of stone fruits in North America. In July 2010, 20 sweet cherry fruit (Prunus avium) of unknown variety with symptoms resembling brown rot were collected from one commercial orchard in Maryland. Each cherry fruit came from a different tree. Symptoms included necrotic areas up to 10 mm in diameter with brown conidia and conidiophores developing from the infection center. Spores from nine symptomatic fruit collected each from different trees of a single orchard were suspended in sterile water, spread onto the surface of 1% agar plates, and incubated at 22°C. After 12 h, single, germinated spores were transferred onto 9-cm petri dishes with potato dextrose agar (PDA). Nine fungal colonies, each from a different fruit, were investigated in three replicates for cultural characteristics on separate petri dishes containing PDA. They were very similar in morphology and grew 12.4 mm per day on average at 22°C, forming branched, monilioid chains of grayish colonies with concentric rings and little sporulation. Rich sporulation was observed on tomato sauce medium (250 ml tomato sauce and 20 g agar in 750 ml water). The lemon-shaped spores had an average size of 15 × 10 μm, which is consistent with M. fructicola. Two colonies were randomly selected to identify the pathogen to the species level using a PCR technique based on cytochrome b sequence amplifications (2). Resulting gel electrophoresis patterns were consistent with M. fructicola. Koch's postulates were fulfilled by inoculating 15 mature sweet cherry fruits of cv. Bing with a conidial suspension (105 spores/ml) of one of the single-spore isolates from cherry. Fruit were stab-inoculated at a point to a depth of 2 mm using a sterile needle. A 10-μl droplet was placed on each wound; control fruit received sterile water without conidia. After 3 days of incubation at room temperature in airtight plastic bags, the inoculated fruit developed typical brown rot symptoms with lesions that were 20.6 mm in diameter. The developing spores on inoculated fruit were confirmed to be M. fructicola. All control fruit remained healthy. The entire detached fruit experiment was repeated 1 week later. M. fructicola is assumed to be the main causal agent of brown rot of sweet cherry in the northeastern United States, but recent studies show that M. laxa is also causing the disease on sweet cherry in many northeastern states (1). For this reason, it is important to delineate species for accurate disease assessments. This study confirms assumptions that M. fructicola is a causal agent of sweet cherry in Maryland. References: (1) K. D. Cox et al. Plant Dis. 12:1584. 2011. (2) J.-M. Hily et al. Pest Manag. Sci. 67:385, 2011.

scholarly journals Genetic Analysis and Discrimination of Sweet Cherry Cultivars and Selections Using Amplified Fragment Length Polymorphism Fingerprints

2002 ◽  
Vol 127 (5) ◽  
pp. 786-792 ◽  
Author(s):  
Lili Zhou ◽  
Frank Kappel ◽  
Cheryl Hampson ◽  
Paul A. Wiersma ◽  
Guus Bakkeren
Keyword(s):  
Fragment Length ◽  
Phylogenetic Trees ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Genetic Relationships ◽  
Breeding Program ◽  
Research Centre ◽  
Aflp Analysis ◽  
Blind Test ◽  
Sweet Cherry Cultivars

Amplified fragment length polymorphisms (AFLPs) were used to analyze the relationships between sweet cherry (Prunus avium L.) cultivars and selections from the breeding program at the Pacific Agri-Food Research Centre in Summerland, Canada. Six pairs of preselected primers were used for the analysis of a total of 67 cultivars and selections. Scoring the absence and presence of 118 polymorphic DNA fragments produced a unique binary code for each cultivar and selection. Two phylogenetic trees were constructed using these 118 polymorphic fragments, one tree for 55 related cultivars and selections from the Summerland breeding program and the other for 23 self-incompatible cultivars of differing origins. The reliability of AFLP DNA fingerprints was confirmed by correlating relationships revealed by AFLP profiles with known genetic relationships of some sweet cherry cultivars and by a blind test for cultivar identification. Results indicate that AFLP analysis is a good technique to evaluate genetic distance and relationships in a sweet cherry breeding population.

13S2009 (Staccato™) sweet cherry

2006 ◽  
Vol 86 (4) ◽  
pp. 1239-1241 ◽  
Author(s):  
Frank Kappel ◽  
Richard A MacDonald ◽  
Rob Brownlee
Keyword(s):  
Key Words ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Fruit Size ◽  
Research Centre ◽  
Fruit Breeding ◽  
Food Research ◽  
The Pacific ◽  
Commercial Testing ◽  
Prunus Avium L

13S2009 (Staccato™) is a very late maturing sweet cherry (Prunus avium L.) that has been released by the Pacific Agri-Food Research Centre (PARC-Summerland), Agriculture and Agri-Food Canada at Summerland, B.C. The release is part of the ongoing sweet cherry breeding program that began in 1936 (Kappel and Lay 1997). Staccato™ is the latest maturing cultivar released from PARC-Summerland and has very good fruit size and firmness. It was released to growers for commercial testing in 2000. Key words: Prunus avium, fruit breeding, cultivars

scholarly journals First Report of Brown Rot Caused by Monilinia fructicola Affecting Peach Orchards in Slovenia

Plant Disease ◽  
2010 ◽  
Vol 94 (9) ◽  
pp. 1166-1166 ◽  
Author(s):  
A. Munda ◽  
M. Viršček Marn
Keyword(s):  
Prunus Persica ◽  
Brown Rot ◽  
Fruit Rot ◽  
Morphological Identification ◽  
Monilinia Fructicola ◽  
Conidial Suspension ◽  
Stone Fruits ◽  
The European Union ◽  
First Report ◽  
Representative Isolate

Monilinia fructicola, the causal agent of brown rot, is a destructive fungal pathogen that affects mainly stone fruits (Prunoideae). It causes fruit rot, blossom wilt, twig blight, and canker formation and is common in North and South America, Australia, and New Zealand. M. fructicola is listed as a quarantine pathogen in the European Union and was absent from this region until 2001 when it was detected in France. In August 2009, mature peaches (Prunus persica cv. Royal Glory) with brown rot were found in a 5-year-old orchard in Goriška, western Slovenia. Symptoms included fruit lesions and mummified fruits. Lesions were brown, round, rapidly extending, and covered with abundant gray-to-buff conidial tufts. The pathogen was isolated in pure culture and identified based on morphological and molecular characters. Colonies on potato dextrose agar (PDA) incubated at 25°C in darkness had an average daily growth rate of 7.7 mm. They were initially colorless and later they were light gray with black stromatal plates and dense, hazel sporogenous mycelium. Colony margins were even. Sporulation was abundant and usually developed in distinct concentric zones. Limoniform conidia, produced in branched chains, measured 10.1 to 17.7 μm (mean = 12.1 μm) × 6.2 to 8.6 μm (mean = 7.3 μm) on PDA. Germinating conidia produced single germ tubes whose mean length ranged from 251 to 415 μm. Microconidia were abundant, globose, and 3 μm in diameter. Morphological characters resembled those described for M. fructicola (1). Morphological identification was confirmed by amplifying genomic DNA of isolates with M. fructicola species-specific primers (2–4). Sequence of the internal transcribed spacer (ITS) region (spanning ITS1 and ITS 2 plus 5.8 rDNA) of a representative isolate was generated using primers ITS1 and ITS4 and deposited in GenBank (Accession No. GU967379). BLAST analysis of the 516-bp PCR product revealed 100% identity with several sequences deposited for M. fructicola in NCBI GenBank. Pathogenicity was tested by inoculating five mature surface-sterilized peaches with 10 μl of a conidial suspension (104 conidia ml–1) obtained from one representative isolate. Sterile distilled water was used as a control. Peaches were wounded prior to inoculation. After 5 days of incubation at room temperature and 100% relative humidity, typical brown rot symptoms developed around the inoculation point, while controls showed no symptoms. M. fructicola was reisolated from lesion margins. Peach and nectarine orchards in a 5-km radius from the outbreak site were surveyed in September 2009 and M. fructicola was confirmed on mummified fruits from seven orchards. The pathogen was not detected in orchards from other regions of the country, where only the two endemic species M. laxa and M. fructigena were present. To our knowledge, this is the first report of M. fructicola associated with brown rot of stone fruits in Slovenia. References: (1) L. R. Batra. Page 106 in: World Species of Monilinia (Fungi): Their Ecology, Biosystematics and Control. J. Cramer, Berlin, 1991. (2) M.-J. Côté et al. Plant Dis. 88:1219, 2004. (3) K. J. D. Hughes et al. EPPO Bull. 30:507, 2000. (4) R. Ioos and P. Frey. Eur. J. Plant Pathol. 106:373, 2000.

scholarly journals The Profile and Content of Polyphenols and Carotenoids in Local and Commercial Sweet Cherry Fruits (Prunus avium L.) and Their Antioxidant Activity In Vitro

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 534 ◽  
Author(s):  
Dominika Średnicka-Tober ◽  
Alicja Ponder ◽  
Ewelina Hallmann ◽  
Agnieszka Głowacka ◽  
Elżbieta Rozpara
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Sweet Cherries ◽  
High Antioxidant Activity ◽  
Positive Correlations ◽  
Sweet Cherry Cultivars ◽  
Prunus Avium L

The aim of this study was to evaluate and compare the content of a number of bioactive compounds and antioxidant activity of fruits of selected local and commercial sweet cherry (Prunus avium L.) cultivars. The experiment showed that the selected cultivars of sweet cherries differ significantly in the content of polyphenolic compounds and carotenoids. The fruits of commercial sweet cherry cultivars were, on average, richer in polyphenols (the sum of phenolic compounds determined chromatographically), flavonoids, as well as anthocyanins and were characterized by higher antioxidant activity when compared to the local, traditional cultivars. In the group of the traditional sweet cherry cultivars, particular attention could be paid to Black Late cv., showing the highest antioxidant activity of fruits. In the group of commercial sweet cherry cultivars, Cordia and Sylvia fruits could be recognized as being rich in bioactive compounds with high antioxidant activity. Yellow skin cultivars were characterized by the highest concentrations of carotenoids. Strong positive correlations between the identified bioactive compounds and antioxidant activity of fruits were also found. Although different cultivars of sweet cherries show a high variability in phenolics and carotenoids profiles as well as in the antioxidant activity of fruits, they all should be, similarly to other types of cherries, recognized as a rich source of bioactive compounds with an antioxidant potential.

Resistance of sweet cherry cultivars to fruit surface pitting

2006 ◽  
Vol 86 (4) ◽  
pp. 1197-1202 ◽  
Author(s):  
Frank Kappel ◽  
Peter Toivonen ◽  
Sabina Stan ◽  
Darrell-Lee McKenzie
Keyword(s):  
Key Words ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Industry Standard ◽  
Sweet Cherries ◽  
Sweet Cherry Cultivars ◽  
Fruit Surface ◽  
Prunus Avium L

A recently developed technique was used to determine the susceptibility to fruit surface pitting of new sweet cherry (Prunus avium L.) cultivars and compare them to an industry standard. The cultivars tested included Bing (industry standard), Cristalina, Lapins, Sandra Rose, Santina, Skeena, Sonata, Staccato, and Sweetheart. Fruit were harvested at commercial maturity, injured, held at 1°C for 2 wk and then rated for fruit surface pitting. The cultivars Lapins, Skeena, Staccato, and Sweetheart had less pitting than Bing. Cristalina and Sonata tended to have similar levels of injury to Bing and Sandra Rose and Santina tended to have more severe pitting than Bing. Key words: Sweet cherries, cultivars, simulated pitting injury

Chilliwack-VR orchardgrass

2006 ◽  
Vol 86 (1) ◽  
pp. 173-175 ◽  
Author(s):  
S. Bittman ◽  
S. N. Acharya ◽  
D. E. Hunt
Keyword(s):  
Dactylis Glomerata ◽  
Mottle Virus ◽  
Research Centre ◽  
Temperate Climate ◽  
Food Research ◽  
The Pacific ◽  
Southern Alberta ◽  
Dactylis Glomerata L ◽  
Cocksfoot Mottle Virus ◽  
Better Than

Chilliwack-VR is a medium-maturing orchardgrass (Dactylis glomerata L.) population with high levels of resistance to Cocksfoot Mottle Virus (CfMV) suited to silage and hay production in regions with a moderate temperate climate. The population was developed at the Pacific Agri-Food Research Centre (PARC), Agriculture and Agri-Food Canada (AAFC), Agassiz, BC, in collaboration with AAFC Research Centre, Lethbridge, AB. In BC trials, Chilliwack-VR yielded 5% more than the check cultivar Potomac without inoculation with CfMV and 23% better than the check when inoculated with the virus. In a southern Alberta trial, Chilliwack-VR survived for 3 yr and yielded as much biomass as a winterhardy check cultivar, Kay. Key words: Orchardgrass, Cocksfoot Mottle Virus resistance, Dactylis glomerata

scholarly journals Inbreeding, Coancestry, and Founding Clones of Sweet Cherries from North America

2004 ◽  
Vol 129 (4) ◽  
pp. 535-543 ◽  
Author(s):  
Cheol Choi ◽  
Frank Kappel
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Breeding Program ◽  
Breeding Programs ◽  
Sweet Cherries

Inbreeding and coancestry coefficients were calculated for 66 sweet cherry (Prunus avium L.) selections released from four breeding programs in North America (HRIO, Vineland, Ont., IAREC, Prosser, Wash., NYSAES, Geneva, N.Y., and PARC, Summerland, B.C.). Highly used founding clones were `Black Heart', `Emperor Francis', `Empress Eugenie', `Napoleon' and `Windsor'. Coefficients of coancestry between all selections and these clones averaged 0.038, 0.045, 0.060, 0.091, and 0.033, respectively. In these five founding clones, coefficients of coancestry in self-compatible selections were over twice as much as those in self-incompatible selections except `Windsor'. In the analysis of coefficients of coancestry between self-incompatible and self-compatible sweet cherry, almost 20% of self-incompatible selections represent more than a half-sib relationship (0.125) to self-compatibles. Increasing and maintaining genetic diversity is needed in sweet cherry breeding program in North America for continued breeding progress.

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