A simplified procedure for verifying and identifying potato cultivars using multiplex PCR

2008 ◽  
Vol 88 (4) ◽  
pp. 583-592 ◽  
Author(s):  
Xiu-Qing Li ◽  
Muhammad Haroon ◽  
Shirlyn E Coleman ◽  
Andrew Sullivan ◽  
Mathuresh Singh ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Fingerprinting ◽  
Solanum Tuberosum L ◽  
Polyacrylamide Gel Electrophoresis ◽  
Potato Cultivar ◽  
Potato Production ◽  
Potato Cultivars ◽  
Correct Identification ◽  
The Public ◽  
Simplified Procedure

Correct identification of potato cultivars and selections is essential to a large and diverse user group. This group includes curators of germplasm repositories, breeders and other researchers, certification program officials, commercial growers, processing industry managers and for some cultivars, the public. Agencies involved in cultivar registration and plant breeders' rights (or patenting) also have a vested interest in correct identification. DNA fingerprinting is an important tool that can be used to describe new or existing cultivars, verify cultivar identity, and resolve cultivar mixtures. Gel-based fingerprints are usually preferred because they are visual and within the technical capacity of most molecular laboratories. In this study, a multiplex PCR protocol "Multiplex SUP" and an improved version "Multiplex SUPN" were developed using four primer pairs (STEM0014 and genes of starch synthase, patatin, and UDP-glucose pyrophosphorylase). The agarose-gel-based Multiplex SUP was successfully used in identifying cultivars from blind samples in a collaborating laboratory, and in pilot tests to verify the identity of introduced cultivars for seed potato production. The Multiplex SUPN, using native polyacrylamide gel electrophoresis (PAGE) with GelRed or ethidium-bromide staining, generated more than 38 polymorphic markers among the potato cultivars tested. The method distinguished 116 cultivars that included many of the public potato cultivars registered in Canada and several protected cultivars that were fingerprinted with permission. The Multiplex SUPN-PAGE method is user friendly and effective, and is recommended for routine potato cultivar verification and identification. Key words: cultivar identification, database development, DNA fingerprinting, GelRed staining, genotyping, polymorphism, Solanum tuberosum L.

DNA fingerprinting and genetic relationships of potato cultivars (Solanum tuberosum L.) commercially grown in Australia

2001 ◽  
Vol 52 (9) ◽  
pp. 911 ◽  
Author(s):  
Daniel A. Isenegger ◽  
Paul W. J. Taylor ◽  
Rebecca Ford ◽  
Peter Franz ◽  
Graeme R. McGregor ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Genetic Similarity ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Solanum Tuberosum L ◽  
Genetic Relationships ◽  
Potato Cultivar ◽  
Potato Cultivars ◽  
Dna Fingerprints ◽  
Banding Patterns

DNA fingerprints of 64 potato cultivars that are commercially grown in Australia were generated using PCR-based RAPD analysis. All 64 cultivars were differentiated by banding patterns obtained from 17 primers that generated 133 polymorphisms. Clonal variants of cvv. Atlantic, Kennebec, Sebago, and Russet Burbank were found to have within-cultivar identical banding patterns. The largest genetic similarity between potato cultivars and the Solanum andigena and Solanum acuale outgroups were 0.5 and 0.4, respectively. The genetic similarity between only potato cultivars ranged from 0.67 to 0.9. Using similarity data a dendrogram was constructed that showed close genetic relationships between a number of cultivars of similar pedigree. This study has shown that DNA fingerprinting is a useful tool for potato cultivar identification, differentiation, and estimating genetic relationships.

scholarly journals Anastomosis Grouping of Rhizoctonia solani Associated with Black Scurf and Stem Canker of Potato in South Africa

Plant Disease ◽  
2004 ◽  
Vol 88 (1) ◽  
pp. 83-83 ◽  
Author(s):  
M. Truter ◽  
F. C. Wehner
Keyword(s):  
South Africa ◽  
Rhizoctonia Solani ◽  
Rating Scale ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Stem Canker ◽  
Potato Production ◽  
Black Scurf ◽  
Chi Square ◽  
Chi Square Analysis

Rhizoctonia disease (black scurf of tubers and stem canker) of potato (Solanum tuberosum L.) caused by Rhizoctonia solani Kühn was first recorded in South Africa in 1918 (3). Although the sclerotial form on tubers is one of the most common potato diseases in the country, it is not known which anastomosis groups (AGs) of R. solani are involved. Between 1999 and 2001, R. solani was isolated from 28 plant and 56 soil samples collected in 7 (Eastern Free State, Gauteng, KwaZulu-Natal, Limpopo, Mpumalanga, Northern Cape, and Sandveld) of the 14 potato-production regions of South Africa and screened for hyphal anastomosis with tester strains of R. solani AG-1 to AG-10 according to Carling et al. (1). Of the 411 isolates from tubers with black scurf symptoms, 408 were AG-3 and three were AG-5. Symptomless tubers yielded two AG-3 isolates and three AG-5 isolates. Of 39 isolates from symptomatic stems and roots, 32 were AG-3, five were AG-4, and two were AG-5. Of the 127 isolates obtained from soil, 86, 28, 7, 3, and 3 were AG-3, AG-4, AG-5, AG-7, and AG-8, respectively. More than one AG was isolated from five of the seven regions. Virulence of 40 isolates representative of the above AGs was determined in triplicate on sprouts growing from seed tubers of potato cultivar Up-to-Date in a sand/soil mixture as described by Carling and Leiner (2) but using cultures grown in cornmeal/sand instead of colonized agar disks as inoculum. Damage to sprouts (lesions, girdling, and death) was assessed after 28 days at 16 to 28°C according to the 0 to 4 rating scale (2). Chi-square analysis of the data indicated that AG-3 was the most virulent, with isolates from sclerotia on tubers and lesions on stems more aggressive than those from symptomless tubers or soil. AG-4 and AG-5 caused significantly less disease than AG-3, but none of the AG-7 and AG-8 isolates showed any virulence to potato sprouts. References: (1) D. E. Carling et al. Phytopathology 77:1609, 1987. (2) D. E. Carling and R. H. Leiner. Phytopathology 80:930, 1990. (3) E. M. Doidge. S. Afr. Fruit Growers 5:6, 1918.

scholarly journals Use of acetylsalicylic acid and agronomic performance of potatoes in Lima region.

2021 ◽  
Author(s):  
Sergio Contreras-Liza
Keyword(s):  
Acetylsalicylic Acid ◽  
Agronomic Traits ◽  
Potato Cultivar ◽  
Specific Effect ◽  
Potato Production ◽  
Potato Cultivars ◽  
Extreme Temperatures ◽  
Oxidative Condition ◽  
Number Of Stems ◽  
Stimulation Of

Abstract Background: Salicylic acid participates in the regulation of the plant's response to a series of environmental stresses such as extreme temperatures, salinity, and oxidative condition of potato production, so it is necessary to determine a safe application dosage for potato in field conditions. Methods: The purpose of the research was to evaluate the effect of increasing doses of acetylsalicylic acid on the agronomic traits of two potato cultivars in Lima, Peru. In a field experiment, an RCBD with 3 replications was used under a split-plot arrangement in which five doses (0.0, 0.2, 0.4, 0.6, and 0.8 mM) of acetylsalicylic acid plus control with Biol (biostimulant) were assigned to plots, and two potato cultivars ('Perricholi' and 'Única') were assigned to subplots. The average temperatures were between 15 and 21°C, the air relative humidity between 61 and 73% and the soil was loam textural class, free of salts. Results: Statistical differences (p <0.05) were found between the two potato cultivars for most of the characters studied. The effects of doses of acetylsalicylic acid showed statistical differences for sprouting, vegetative vigor, and percentage of flowering; likewise, significant interactions (p <0.05) were shown between potato cultivars and dose of acetylsalicylic acid for the number of stems per plant and percentage of flowering, which would indicate a specific effect for the dose of acetylsalicylic acid in each potato cultivar. Conclusion: No dose of the product significantly affected the potato yield in two potato cultivars however, the effect of the dosage of acetylsalicylic acid improved the performance of the crop in terms of sprouting in the field, vegetative vigor, a greater number of stems per plant as well as stimulation of flowering, with respect to control.

Internal gas atmospheres, ethanol, and leakage of electrolytes from a flood-tolerant and a flood-susceptible sweet potato cultivar as influenced by anaerobiosis

1983 ◽  
Vol 61 (12) ◽  
pp. 3399-3404 ◽  
Author(s):  
Ling A. Chang ◽  
Larry K. Hammett ◽  
David M. Pharr
Keyword(s):  
Sweet Potato ◽  
Electrolyte Leakage ◽  
Potato Cultivar ◽  
Enriched Environment ◽  
Potato Cultivars ◽  
Susceptible Cultivar ◽  
Low Concentrations ◽  
Gas Atmosphere ◽  
High Concentrations

The postanaerobic behavior of sweet potato roots from a flood-tolerant cultivar, 'Centennial,' and a flood-susceptible cultivar, 'Caromex,' was studied. High concentrations of CO2 and low concentrations of O2 were present in the internal atmosphere of 'Caromex' roots even after the anaerobically pretreated roots were restored to air for a few days, whereas in 'Centennial,' the internal gas atmosphere was less affected by anoxia. Ethanol accumulation in 'Caromex' was consistently higher than in 'Centennial,' and the postanaerobic changes were different between cultivars. An inducation of electrolyte leakage was observed from both cultivars immediately after roots were exposed to a CO2-enriched environment for 48 h. The leakage became greater in 'Caromex' after a 3-day aerobic exposure. In 'Centennial,' leakage of electrolytes due to CO2 treatment diminished at the end of 3 days. Application of ethanol to the discs had no immediate effect on electrolyte leakage in either cultivar under a N2 environment. However, anaerobiosis alone resulted in higher electrolyte leakage. It remains to be determined that the postanaerobic patterns of 'Caromex' and 'Centennial' are characteristic of flood-susceptible and flood-tolerant sweet potato cultivars in general.

Genetic diversity of Meloidogyne spp. parasitising potato in Brazil and aggressiveness of M. javanica populations on susceptible cultivars

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Israel L. Medina ◽  
Cesar B. Gomes ◽  
Valdir R. Correa ◽  
Vanessa S. Mattos ◽  
Philippe Castagnone-Sereno ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Potato Production ◽  
Potato Cultivars ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Nematode Reproduction ◽  
Meloidogyne Spp ◽  
Prevalent Species ◽  
Reproduction Factor

Root-knot nematodes (Meloidogyne spp.) significantly impact potato production worldwide and in Brazil they are considered one of the most important group of nematodes affecting potatoes. The objectives of this study were to survey Meloidogyne spp. associated with potatoes in Brazil, determine their genetic diversity and assess the aggressiveness of M. javanica on two susceptible potato cultivars. Fifty-seven root-knot nematode populations were identified using esterase phenotyping, including Meloidogyne javanica, M. incognita, M. arenaria and M. ethiopica. Overall, root-knot nematodes were present in ca 43% of sampled sites, in which M. javanica was the most prevalent species, and the phenotypes Est J3, J2a and J2 occurred in 91.2, 6.7 and 2.1% of the positive samples, respectively. Other species, such as M. incognita, M. arenaria and M. ethiopica, were found less frequently and occurred at rates of 6.4, 4.3 and 2.1% of the samples, respectively. Sometimes, M. javanica was found in mixtures with other root-knot nematodes in ca 10.6% of sites containing Meloidogyne. After confirming the identification of 17 isolates of M. javanica and one isolate each of M. incognita, M. arenaria and M. ethiopica by SCAR markers, the populations were used to infer their genetic diversity using RAPD markers. Results revealed low intraspecifc genetic diversity among isolates (13.9%) for M. javanica. Similarly, M. javanica sub-populations (J2a) clustered together (81% of bootstrap), indicating subtle variation from typical J3 populations. The aggressiveness of four populations of M. javanica from different Brazilian states on two susceptible potato cultivars was tested under glasshouse conditions. Results indicated differences in aggressiveness among these populations and showed that potato disease was proportional to nematode reproduction factor.

scholarly journals AN ALTERNATIVE APPROACH TO IDENTIFY KEY INDUSTRIES: ISSUES TO SELECTION CRITERIA

2014 ◽  
Vol 15 (3) ◽  
pp. 577-598
Author(s):  
Abul Quasem Al-Amin ◽  
Abdul Hamid Jaafar
Keyword(s):  
Selection Criteria ◽  
Public Investment ◽  
Correct Identification ◽  
The Public ◽  
Study Approach ◽  
Sectoral Planning ◽  
Alternative Approach ◽  
The Subject ◽  
Key Industries ◽  
High Degree

Within a process of modeling exercise, this study aimed to understand appropriate selection criteria to identify key industries. There are many key sector identification linkage measures in the subject matter and sensitivity issue among them can be tricky because many of these measures differ only slightly but can result in outcomes that are quite dissimilar. With this background, we proposed an alternate approach that helps to resolve this issue. The proposed approach utilizes in this study by five sub-methods and high degree of the frequency of their occurrences in sub-methods to determine the key sectors. The study approach is applied to Malaysia as the public sector investment remains a large share in the national economy, like other developing countries, and the correct identification is still a challenge for sectoral planning. The experiences from this study can be used to guide appropriate public investment in Malaysia and elsewhere with similar economic forms.

scholarly journals Comprehensive Genome-Wide Analysis of The Catalase Enzyme Toolbox In Potato (Solanum Tuberosum L.)

2021 ◽  
Author(s):  
Rania Jbir Koubaa ◽  
Mariem Ayadi ◽  
Mohamed Najib Saidi ◽  
Safa Charfeddine ◽  
Radhia Gargouri Bouzid ◽  
...  
Keyword(s):  
Stress Responses ◽  
Expression Profiling ◽  
Phylogenetic Trees ◽  
Solanum Tuberosum L ◽  
Potato Cultivar ◽  
Promoter Sequence ◽  
3D Protein Structure ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
A Genome

Abstract As antioxidant enzymes, catalase (CAT) protects organisms from oxidative stress via the production of reactive oxygen species (ROS). These enzymes play important roles in diverse biological processes. However, little is known about the CAT genes in potato plants despite its important economical rank of this crop in the world. Yet, abiotic and biotic stresses severely hinder growth and development of the plants which affects the production and quality of the crop. To define the possible roles of CAT genes under various stresses, a genome-wide analysis of CAT gene family has been performed in potato plant.In this study, the StCAT gene’s structure, secondary and 3D protein structure, physicochemical properties, synteny analysis, phylogenetic tree and also expression profiling under various developmental and environmental cues were predicted using bioinformatics tools. The expression analysis by RT-PCR was performed using commercial potato cultivar. Three genes encoding StCAT that code for three proteins each of size 492 aa, interrupted by seven introns have been identified in potatoes. StCAT proteins were found to be localized in the peroxisome which is judged as the main H2O2 cell production site during different processes. Many regulating cis-elements related to stress responses and plant hormones signaling were found in the promoter sequence of each gene. The analysis of motifs and phylogenetic trees showed that StCAT are closer to their homologous in S. lycopersicum and share a 41% – 95% identity with other plants’ CATs. Expression profiling revealed that StCAT1 is the constitutively expressive member; while StCAT2 and StCAT3 are the stress-responsive members.

scholarly journals The Improvement of Mini Tuber Production of Granola Potato Cultivar in Aeroponics System

2021 ◽  
Vol 13 (1) ◽  
pp. 77-83
Author(s):  
Sugiyono Sugiyono ◽  
Lucky Prayoga ◽  
Elly Proklamasiningsih ◽  
Khavid Faozi ◽  
Rendie Prasetyo
Keyword(s):  
Potato Cultivar ◽  
Potato Production ◽  
Tuber Formation ◽  
Planting Density ◽  
Food Crop ◽  
Important Food ◽  
High Quality ◽  
Plant Materials ◽  
Tuber Production ◽  
Surrounding Areas

Potato is an important food crop in both developed and developing countries and it is the fourth most important food crop after wheat, maize, and rice. Potato production in Indonesia has decreased in recent years, mainly due to the use of low quality tuber seed. The objective of this research was to determine the effect of plant materials, types of nutrients, and planting density on mini tuber formation, as well as to determine the best aeroponics conditions for mini tuber formation of Granola cultivar of potato. This research has been carried out experimentally using a Split-split Plot Design. The main plot was planting materials which consisted of plantlet and knol. The subplot was the type of nutrition media which consisted of Farran nutrition, Otazu nutrition, and AB-commercial mix. The sub-sub-plot was planting density which consisted of 50, 75, and 100 plants/m2. The research results showed that knol planted in aeroponics system on AB-commercial mix at a planting density of 75 plants/m2 resulted in the best mini-tuber production. In this study, for the first time three important factors for potato mini-tuber production have thoroughly been investigated in Indonesia. This finding will be used by Kledung Horticultural Seed Garden, Temanggung, for mass production of high-quality mini-tuber seed, which in turn will provide sufficient tuber seed for the farmer in its surrounding areas. By increasing the production of high-quality tuber seed, it is expected that it will contribute to the improvement of potato productivity and increase Indonesian potato production.

scholarly journals Production high class of micro tuber potato seeds (Solanum tuberosum L.). by Using tissue culture technique

2009 ◽  
Vol 3 (2) ◽  
pp. 99-106
Author(s):  
A.A. Al-jibouri ◽  
A.A. Al-salhay
Keyword(s):  
Tissue Culture ◽  
Solanum Tuberosum L ◽  
Culture Technique ◽  
Potato Cultivars ◽  
Condition Numbers ◽  
Mass Propagation ◽  
High Class ◽  
Dormancy Period ◽  
Elisa Technique

The aim of this investigation was produced micro tubers of four potato cultivars Premiere, Bintje, Estima and Escort in vitro. Apical meristems (0.2-0.4 mm) of potato cultivars were excised and cultured on nutrient medium and incubated at 24±2 Cº and 1000 lux light intensity for 16 hrs per day. The developing plantlets were examined serological by using ELISA technique to eliminate the viral infected plantlets. The virus-free plantlets were chopped into pieces with single bud and re cultured on fresh medium for mass propagation. For micro tubers formation in test tubes, the cultures were transferred to another medium containing a high percent of sucrose (60g/L) with different concentrations of kinetin; the cultures were incubated under 16±2 Cº and 8 hrs photoperiod. The plantlets formed micro tubers after 8-10 weeks from culturing. The results showed significant differences among cultivar’s in their response to in vitro culture and micro tubers formation. The results also showed that the kinetin concentration had significant effect on micro tubers, and 1mg/l kinetin concentration was the best. The micro tubers were stored for 10 week at 4Cº to break down the dormancy period, and gave 100% germination under nursery condition. Numbers of tubers derived from micro tubers and normal tubers of these cultivars were compared at the end of season.

scholarly journals Comparative study on meristem culture of three potato cultivars diamant, cardinal and granula and their shoot formation

2016 ◽  
Vol 1 (3) ◽  
pp. 537-544
Author(s):  
Marufa Khatun ◽  
Md Shahadat Hossain ◽  
Md Khalekuzzan ◽  
Amina Rownaq
Keyword(s):  
Comparative Study ◽  
Growth Response ◽  
Potato Cultivar ◽  
Shoot Formation ◽  
Shoot Tips ◽  
Potato Cultivars ◽  
Meristem Culture ◽  
Ms Medium ◽  
Surface Sterilization ◽  
Apical Meristems

The main aim of this study was to analyze the comparative study on meristem culture of three important potato cultivars, diamant, cardinal and granula and their shoot formation using meristem tips. Apical meristems were isolated from shoot tips of 25-35 days old field grown plants. After surface sterilization the meristems (0.3 mm) were isolated from the shoot tips. After isolation the apical meristems were placed quickly on “M” shaped filter paper bridge in culture tubes containing liquid MS medium supplemented with various concentrations and combinations of different plant growth regulators. Meristems showed their first growth response by increase in size and became greenish white in colour. They continued their growth and developed shoots with roots. In the present investigation, among the three cultivars of potato, cardinal were the best responsive cultivars for the resuming new growth of cultured meristems on MS medium supplemented with KIN (0.4 mg l-1) + GA3 (0.5 mg l-1). Cultivar diamant showed comparatively better growth response in the primary culture and granula showed better results in shoot length formation in MS0 medium. Cultivar cardinal proved to be best potato cultivar in case of meristem culture than diamant and granula cultivars.Asian J. Med. Biol. Res. December 2015, 1(3): 537-544

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