DNA fingerprinting and genetic relationships of potato cultivars (Solanum tuberosum L.) commercially grown in Australia

2001 ◽  
Vol 52 (9) ◽  
pp. 911 ◽  
Author(s):  
Daniel A. Isenegger ◽  
Paul W. J. Taylor ◽  
Rebecca Ford ◽  
Peter Franz ◽  
Graeme R. McGregor ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Genetic Similarity ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Solanum Tuberosum L ◽  
Genetic Relationships ◽  
Potato Cultivar ◽  
Potato Cultivars ◽  
Dna Fingerprints ◽  
Banding Patterns

DNA fingerprints of 64 potato cultivars that are commercially grown in Australia were generated using PCR-based RAPD analysis. All 64 cultivars were differentiated by banding patterns obtained from 17 primers that generated 133 polymorphisms. Clonal variants of cvv. Atlantic, Kennebec, Sebago, and Russet Burbank were found to have within-cultivar identical banding patterns. The largest genetic similarity between potato cultivars and the Solanum andigena and Solanum acuale outgroups were 0.5 and 0.4, respectively. The genetic similarity between only potato cultivars ranged from 0.67 to 0.9. Using similarity data a dendrogram was constructed that showed close genetic relationships between a number of cultivars of similar pedigree. This study has shown that DNA fingerprinting is a useful tool for potato cultivar identification, differentiation, and estimating genetic relationships.

A simplified procedure for verifying and identifying potato cultivars using multiplex PCR

2008 ◽  
Vol 88 (4) ◽  
pp. 583-592 ◽  
Author(s):  
Xiu-Qing Li ◽  
Muhammad Haroon ◽  
Shirlyn E Coleman ◽  
Andrew Sullivan ◽  
Mathuresh Singh ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Fingerprinting ◽  
Solanum Tuberosum L ◽  
Polyacrylamide Gel Electrophoresis ◽  
Potato Cultivar ◽  
Potato Production ◽  
Potato Cultivars ◽  
Correct Identification ◽  
The Public ◽  
Simplified Procedure

Correct identification of potato cultivars and selections is essential to a large and diverse user group. This group includes curators of germplasm repositories, breeders and other researchers, certification program officials, commercial growers, processing industry managers and for some cultivars, the public. Agencies involved in cultivar registration and plant breeders' rights (or patenting) also have a vested interest in correct identification. DNA fingerprinting is an important tool that can be used to describe new or existing cultivars, verify cultivar identity, and resolve cultivar mixtures. Gel-based fingerprints are usually preferred because they are visual and within the technical capacity of most molecular laboratories. In this study, a multiplex PCR protocol "Multiplex SUP" and an improved version "Multiplex SUPN" were developed using four primer pairs (STEM0014 and genes of starch synthase, patatin, and UDP-glucose pyrophosphorylase). The agarose-gel-based Multiplex SUP was successfully used in identifying cultivars from blind samples in a collaborating laboratory, and in pilot tests to verify the identity of introduced cultivars for seed potato production. The Multiplex SUPN, using native polyacrylamide gel electrophoresis (PAGE) with GelRed or ethidium-bromide staining, generated more than 38 polymorphic markers among the potato cultivars tested. The method distinguished 116 cultivars that included many of the public potato cultivars registered in Canada and several protected cultivars that were fingerprinted with permission. The Multiplex SUPN-PAGE method is user friendly and effective, and is recommended for routine potato cultivar verification and identification. Key words: cultivar identification, database development, DNA fingerprinting, GelRed staining, genotyping, polymorphism, Solanum tuberosum L.

Phylogenetic relationships of five morphological groups of hexaploid wheat (Triticum aestivum L. em Thell.) based on RAPD analysis

Genome ◽  
2000 ◽  
Vol 43 (4) ◽  
pp. 724-727 ◽  
Author(s):  
Wenguang Cao ◽  
G Scoles ◽  
P Hucl ◽  
R N Chibbar
Keyword(s):  
Hexaploid Wheat ◽  
Phylogenetic Relationships ◽  
Genetic Similarity ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Triticum Aestivum L ◽  
Similarity Coefficients ◽  
Morphological Classification ◽  
Wild Wheat

The genetic relationships among the five groups of hexaploid wheat: common, spelta, macha, vavilovii, and semi-wild wheat (SWW) are not clear. Random amplified polymorphic DNA (RAPD) analysis was used to assess phylogenetic relationships among these five morphological groups of hexaploid wheat. RAPD data were analyzed using the NTSYS-PC computer program to generate Jaccard genetic similarity coefficients. A dendrogram based on RAPD analysis grouped 15 accessions into five distinct clusters. These results are in agreement with those based on morphological classification, suggesting that common wheat is most closely related to SWW, followed by spelta, vavilovii, and macha.Key words: RAPD, macha, spelta, vavilovii, semi-wild wheat, phylogenetic relationships.

Development of an identification scheme for Canadian registered oat cultivars using RAPDs

1998 ◽  
Vol 78 (4) ◽  
pp. 605-610 ◽  
Author(s):  
Eduardo Guillin ◽  
Bernard R. Baum ◽  
Subbaiah Mechanda
Keyword(s):  
Key Words ◽  
Dna Fingerprinting ◽  
Cultivar Identification ◽  
Identification Key ◽  
Dna Fingerprints ◽  
Key Words Dna ◽  
Identification Scheme ◽  
Rapd Fingerprints ◽  
Oat Cultivars ◽  
Single Seeds

DNA fingerprints for all 53 oat cultivars registered in Canada were generated using random amplified polymorphic DNAs (RAPDs). Repeatability and reliability of the PCR-RAPD fingerprints were confirmed on up to 20 single seeds or seedlings, from breeders seed of five cultivars. An identification key was computer generated for the 53 cultivars. Twenty-nine potentially diagnostic bands were scored on the 53 cultivars to generate the key, but only 13 were found useful and sufficient by the computer generating key program. The identification scheme lends itself to be online. Further research is required to complete the scheme for routine cultivar identification and verification in Canada. The problems that need to be investigated are discussed. Key words: DNA fingerprinting, oat cultivars, RAPD, identification key

scholarly journals Identification of the Edible Fig `Bianco del Cilento' by Random Amplified Polymorphic DNA Analysis

HortScience ◽  
1999 ◽  
Vol 34 (7) ◽  
pp. 1263-1265 ◽  
Author(s):  
U. Galderisi ◽  
M. Cipollaro ◽  
G. Di Bernardo ◽  
L. De Masi ◽  
G. Galano ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Southern Italy ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Accurate Identification ◽  
Banding Patterns ◽  
Campania Region ◽  
Quality Of Products

Random amplified polymorphic DNA (RAPD) analysis is currently used to estimate genetic relationships in plants. We have used RAPD analysis to distinguish six different cultivars of Ficus carica, and several of their clones, that are widespread in the Campania Region of Southern Italy. Among these cultivars, `Bianco del Cilento' has unique characteristics, and is particularly useful for drying and for the manufacture of syrups. The protection of this cultivar is important to the Campania Region. We have utilized molecular markers to allow accurate identification of this cultivar, making it possible to control the quality of products and prevent fraudulent commerce. DNA was extracted from leaves and amplified by PCR using random oligonucleotide primers. The amplification patterns obtained with five decamer primers were useful for distinguishing all six cultivars analyzed. `Bianco del Cilento' was identified by two primers. The banding patterns were scored and used in similarity value calculations to estimate genetic relationships.

scholarly journals Potato cultivar identification using molecular markers

2010 ◽  
Vol 45 (1) ◽  
pp. 110-113 ◽  
Author(s):  
Patricia Marchezi Rosa ◽  
Tatiana de Campos ◽  
Adna Cristina Barbosa de Sousa ◽  
Danilo Augusto Sforça ◽  
Gisele Abigail Montan Torres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Microsatellite Markers ◽  
Cultivar Identification ◽  
Potato Cultivar ◽  
Polyacrylamide Gel ◽  
Potato Cultivars ◽  
Varietal Identification ◽  
Identification And Characterization

The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.

The application of RAPD markers for potato cultivar identification

1997 ◽  
Vol 48 (8) ◽  
pp. 1213 ◽  
Author(s):  
Rebecca Ford ◽  
Paul W. J. Taylor
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapd Markers ◽  
Cultivar Identification ◽  
Rapd Analysis ◽  
Rapd Marker ◽  
Potato Cultivar ◽  
Specific Marker ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Polymorphic Bands

Where morphological techniques had failed, closely related potato cultivars were differentiated using random amplified polymophic DNA (RAPD) analysis based on the polymerase chain reaction. Total gemomic DNA was extracted from young sprout tissue from harvested tubers. Of 63 10-mer oligonucleotide primers screened, 51 primers produced a total of 256 amplification products of which 33 were polymorphic between the cultivars assessed. Polymorphic bands were selected to produce cultivar-specific markers to identify correctly suspect material in commercial plantings. Furthermore, a cultivar-specific RAPD marker was shown to be sufficiently robust and stringent for the identification of potential cultivar contamination in the field. DNA titration experiments revealed that a specific marker for cv. Sebago could be detected in a DNA admixture at a ratio of 1× cv. Sebago to 5 × cv. Exton. A commercial block of cv. Exton was thus randomly sampled and the level of cultivar purity assessed.

Genetic relationships of Japanese potato cultivars assessed by RAPD analysis

1994 ◽  
Vol 71 (8) ◽  
pp. 535-546 ◽  
Author(s):  
K. Hosaka ◽  
M. Mori ◽  
K. Ogawa
Keyword(s):  
Rapd Analysis ◽  
Genetic Relationships ◽  
Potato Cultivars

scholarly journals Using SSR to Evaluate the Genetic Diversity of Potato Cultivars from Yunnan Province (SW China)

2014 ◽  
Vol 56 (1) ◽  
pp. 16-27 ◽  
Author(s):  
Hong Liao ◽  
Huachun Guo
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Similarity ◽  
Yunnan Province ◽  
Genetic Relationships ◽  
Potato Cultivars ◽  
Good Resolution ◽  
Ssr Analysis ◽  
Low Genetic Diversity ◽  
Polymorphic Bands

Abstract The genetic diversity of potato cultivars collected from Yunnan Province was evaluated using 24 pairs of SSR markers. SSR analysis of 24 pairs of primers showed varying degrees of polymorphism among the 85 cultivars: 297 of the 304 bands were polymorphic. The primers yielded between 5 (STM2028) and 19 (StI029) bands (mean 12). The ratio of polymorphic bands ranged from 83.33% to 100% (mean 97.75%). Polymorphism information content (PIC) varied from 69.31% to 93.67% (mean 86.47%). Genetic similarity ranged from 0.5987 to 0.7632, indicating relatively low genetic diversity in the potato cultivars from Yunnan Province. Cluster analysis by UPGMA and PCA clearly delineated the genetic relationships of all cultivars; 83 of the 85 cultivars could be discriminated by only two pairs of primers, STM0030 and STM1104. The high polymorphism and good resolution of the primers used in this study make them good tools for discriminating potato cultivars.

scholarly journals Distinguishing Poinsettia Cultivars and Evaluating Their Genetic Relationships using DNA Fingerprinting

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 500A-500
Author(s):  
Terri Woods Starman ◽  
Shane Abbitt
Keyword(s):  
Dna Fingerprinting ◽  
Numerical Taxonomy ◽  
Genetic Relatedness ◽  
Cultivar Identification ◽  
Genetic Relationships ◽  
Dna Amplification ◽  
Euphorbia Pulcherrima ◽  
Pedigree Data ◽  
Analysis System ◽  
Distance Estimator

The objective was to distinguish between cultivars and evaluate genetic relatedness of poinsettia (Euphorbia pulcherrima) using two methods of DNA fingerprinting—DNA Amplification Fingerprinting (DAF) and Arbitrary Signatures from Amplification Profiles (ASAP). Eleven red poinsettia cultivars were studied, including `Celebrate 2', `Darlyne', `Freedom Red', `Lilo', `Nutcracker Red', `Peterstar Red', `Petoy', `Red Sails', `Supjibi', `V-14 Glory', and `V-17 Angelika'. Amplification was with 10 octamer primers. Gels were visually scored for presence or absence of bands. The 10 primers generated 336 bands. The average number of bands (≈1000 bp) per primer was 34 ranging from 19 to 43. Thirty-one percent of bands were polymorphic and distinguished between each cultivar. The number of unique profiles varied from two to nine. Genetic relationships were evaluated by SAHN cluster analysis based on the distance estimator of Jaccard using the NTSYS-pc program (Numerical taxonomy and multivariate analysis system, version 1.8). The resulting dendrogram closely agreed with known pedigree data. ASAP analysis was used to further assess cultivar identification of two cultivars that were genetically and morphologically similar. Markers were found that separated `Nutcracker Red' and `Peterstar Red'. ASAP analysis separated cultivars within the Freedom series that DAF failed to distinguish. Two cultivars in the Freedom series, `Jingle Bells' and `Marble', were characterized from other cultivars in the series with ASAP.

scholarly journals Taxonomic variation among Schinus molle L. plants associated with a slight change in elevation

2017 ◽  
Vol 24 (2) ◽  
pp. 205-214
Author(s):  
Abeer Al-Andal ◽  
Mahmoud Moustafa ◽  
Suliman Alruman
Keyword(s):  
Genetic Similarity ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dna Fingerprints ◽  
Similarity Coefficients ◽  
Tree Analysis ◽  
Schinus Molle ◽  
Plant Taxon ◽  
Maximum Similarity ◽  
Rapd And Issr

This study examined the degree of variations in DNA fingerprints associated with slight altitudinal change of Schinus molle grown in Abha region, Saudi Arabia. Seven populations from Schinus molle plants located at 2193.0, 2246.0, 2197.7, 2441.0, 2372.0, 2250.6 and 2175.0 meters had been investigated. The degree of genetic variability was evaluated using random amplified polymorphic DNA (RAPD), mixed RAPD and intersimple sequence repeat markers (ISSR). The genetic similarity coefficients from RAPD analysis revealed the maximum similarity value (89.9%) was between population at 2250.6 m and population at 2175.0 m. The genetic similarity coefficients from mixed RAPD primers displayed the highest similarity value (87.6%) between population at 2246.0 m and population at 2197.7 m. Similarity coefficients from ISSR analysis revealed the highest similarity value (86.2%) among populations at 2193.0 m, 2246.0 m, 2441.0 m and at 2250.6 m. Super tree analysis (RAPD + mixed RAPD + ISSR) showed the highest similarity value (85.5%) between population at 2441.0 m and population at 2250.6 m. In conclusion, marker systems including RAPD, mixed RAPD and ISSR, alone or combined can be effectively used in determining the genetic relationship among Schinus molle plants even at very close populations.Bangladesh J. Plant Taxon. 24(2): 205–214.

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