Lambs produced from cryopreserved sheep embryos derived by in vitro fertilization of aspirated oocytes

1996 ◽  
Vol 76 (3) ◽  
pp. 465-467 ◽  
Author(s):  
N. Songsasen ◽  
A. Martino ◽  
S. P. Leibo ◽  
S. Walmsley ◽  
J. W. Pollard ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Ethylene Glycol ◽  
Key Words ◽  
In Vitro Maturation ◽  
Oocyte Cryopreservation ◽  
Vitro Fertilization

A total of 104 oocytes were aspirated from 11 mature ewes and 4 prepubertal lambs. After in vitro maturation, fertilization and culture, 11 of the 104 oocytes developed into blastocysts. These were cryopreserved in 1.5 M ethylene glycol. After being thawed, all were transferred into four recipients, two of which became pregnant and delivered a normal lamb. Key words: Oocyte, cryopreservation, in vitro maturation and fertilization, sheep, embryo

An attempt at sperm-mediated gene transfer in mice and chickens

1991 ◽  
Vol 71 (2) ◽  
pp. 287-291 ◽  
Author(s):  
J. S. Gavora ◽  
B. Benkel ◽  
H. Sasada ◽  
W. J. Cantwell ◽  
P. Fiser ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Gene Transfer ◽  
Transgenic Mice ◽  
Key Words ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Laboratory Mice ◽  
Vitro Fertilization ◽  
Bacterial Plasmid

Experiments were carried out to transform laboratory mice and domestic chickens by use of sperm incubated with bacterial plasmid DNA. Following demonstration of "uptake" of such DNA by sperm of both species, attempts were made to replicate a previously published procedure (Lavitrano et al. 1989, Cell 57: 717–723) for producing transgenic mice through in vitro fertilization (IVF). Also, female mice and hens were inseminated (AI) with sperm which had been incubated in a DNA solution. Such incubation did not influence the fertility or hatchability of the hens' eggs. However, no transformed progeny were detected among 45 mice produced by IVF or among 69 mice and 470 chickens produced by AI. Key words: Sperm-mediated DNA transfer, mice, chickens

A Comparison of In Vitro Maturation and In Vitro Fertilization for Women With Polycystic Ovaries

2002 ◽  
Vol 100 (4) ◽  
pp. 665-670 ◽  
Author(s):  
Tim J. Child ◽  
Simon J. Phillips ◽  
Ahmad Kamal Abdul-Jalil ◽  
Bulent Gulekli ◽  
Seang Lin Tan
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Polycystic Ovaries ◽  
Vitro Fertilization
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