Effect of level of dietary potassium on the absorption and excretion of calcium and magnesium by lactating cows

1994 ◽  
Vol 74 (3) ◽  
pp. 503-509 ◽  
Author(s):  
L. J. Fisher ◽  
N. Dinn ◽  
R. M. Tait ◽  
J. A. Shelford
Keyword(s):  
Water Consumption ◽  
Urine Output ◽  
Rumen Fluid ◽  
Latin Square ◽  
Experimental Period ◽  
Lower Amount ◽  
Lactating Cows ◽  
High K ◽  
Calcium Magnesium ◽  
Low K

This study was undertaken with the objective of providing more basic information on the absorption and excretion of calcium (Ca) and magnesium (Mg) by lactating cows when high levels of potassium (K) are fed. Employing potassium carbonate as the source of supplemental K, total mixed rations were formulated to contain 1.6 (low), 3.1 (medium) and 4.6% (high) K. The three rations were fed to 15 Holstein cows in mid-lactation in a 3 × 3 Latin square experiment replicated five times. The experimental periods were 21 d in length. Blood, rumen and milk samples were obtained during the 2nd and 3rd wk of each experimental period and two cows from each treatment group were subjected to total collection procedures for feces and urine during the last 4 d of each experimental period. Dry matter intake was 23.0, 23.8 and 22.3 kg d−1 for the cows fed the low, medium and high K diets, respectively. Milk yield was lower (P < 0.05) for cows fed the high compared to the low and medium K diets. Level of K in the diet did not influence the fat and protein content of the milk but Ca content was lower (P < 0.05) for cows fed the medium and high K diets compared with those fed the low K diet. Plasma K levels were 15.0, 15.7 and 16.2 mg dL−1 while plasma Mg levels were 2.35a, 2.25ab and 2.17b mg dL−1 for cows on the low, medium and high K diets, respectively. The acetate to propionate ratio in the rumen fluid was wider (P < 0.05) for the cows fed the medium and high K diets compared with those fed the low K diet. The apparent absorption of Mg but not Ca was reduced with increasing levels of K in the diet. Both water consumption and urine output were greater (P < 0.05) for the cows fed the medium and high K diet compared with those fed the low K diet. The concentration of Ca and Mg in the urine dropped markedly and total excretion of Ca and Mg was lower (P < 0.05) for cows fed the medium and high K diets compared with those fed the low K diet. It was concluded from the results of this study that high K levels in the diet interfere with absorption of Mg but not Ca. However, K did appear to interfere with the utilization of Ca as indicated by the lower amount of Ca in the milk and urine. Increasing K levels in the diet increased water consumption and urine output which has major implications for waste management. Key words: Lactating cows, excess potassium, calcium, magnesium

PSVII-14 Feeding carbohydrases to grazing beef cattle under different nutritional strategies on dry season: Ruminal fermentation

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 449-450
Author(s):  
Anderson Acosta ◽  
Guilherme S Vasconcellos ◽  
Alexandre Perdigão ◽  
Victor Valério de Carvalho ◽  
Tiago S Acedo ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Experimental Period ◽  
Dry Season ◽  
Ruminal Fermentation ◽  
Beta Glucanase ◽  
Randomized Design ◽  
Nutritional Strategy ◽  
Completely Randomized Design ◽  
Nutritional Strategies

Abstract The aim with this trial was to evaluate two nutritional plans with the addition of carbohydrases enzyme blend (CEB) on ruminal fermentation of grazing Nellore bulls in dry season. Five rumen cannulated Nellore steers (BW = 350 kg) were used. Animals were randomly assigned to a treatment sequence in a 5×5 Latin square experiment with 15 d-periods (10 d to diet adaptation and 5 d for data collection), following a completely randomized design, being the treatments: mineral-proteic supplementation (Fosbovi® Proteico 35; PS) offered at 0,1% BW (0 or 4.75 g/animal/day of CEB) or mineral-protein-energetic supplementation (Fosbovi® Proteico-Energético 25; PES) offered at 0.3% BW (0, 4.75 or 9.50 g/animal/day of CEB). The CEB was mainly composed by beta-glucanase and xylanase enzymes (Ronozyme® VP and Ronozyme® WX). Both enzymes and supplements were provided by DSM Nutritional Products Brasil S.A. Rumen fluid samples were collected on the last day of each experimental period before (0), and 2, 4, 6, and 8 h after supplements were offered. Data were analyzed using PROC MIXED of SAS. Steers fed PSE had greater (P ≤ 0.041) rumen concentration of acetate, propionate, and butyrate, and lower (P = 0.028) acetate to propionate ratio in comparison with animals fed PS. Rumen acetate concentrations were 61.6 and 58.8 mmol/L and rumen propionate concentrations were 15.2 and 13.7 mmol/L for animals under the PSE and PS, respectively. A quadratic effect (P = 0.048) for carbohydrases enzyme blend was observed in NH3-N ruminal concentration of PES animals, where 4.75 g/day level presented the lower concentrations compared to control (24.13 vs. 32.20 mg/dL). Furthermore, rumen concentrations of acetate (P = 0.002) and propionate (P = 0.021) linearly increased with enzymes inclusion for PSE plan. PSE nutritional strategy positively influence ruminal fermentation of Nellore steers in dry season and its effects can be enhanced with CEB inclusion of 4.75 g/day.

scholarly journals 79 Effect of source and dietary concentration of trace minerals on serum mineral status, ruminal pH, and ruminal volatile fatty acids in lactating Holstein dairy cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 71-71
Author(s):  
Nicole T Briggs ◽  
Bayissa Hatew ◽  
Michael A Steele
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Experimental Period ◽  
Rumen Fermentation ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Mineral Status ◽  
Ruminal Ph ◽  
Dietary Concentration

Abstract Inorganic sources of trace minerals are commonly supplemented in dairy cow diets; however, there has been an increase in the supplementation of minerals complexed with organic compounds. These organic trace minerals are thought to have greater bioavailability which may enhance rumen fermentation and absorption. The objective of this study was to assess the effects of dietary concentration and source of supplemental trace minerals on serum trace mineral status and rumen fermentation. Six lactating Holstein cows were used in a 6 x 6 Latin square design with a 23-day adaptation and 5-day experimental period. Cows were fed the same basal diet daily except for the difference in source [organic (ORG) versus inorganic (INO)] and concentration (50%, 100%, and 200% based on NRC recommendations) of trace mineral supplemented. During the experimental period feed intake and blood were collected daily. Rumen fluid was collected on the final two days of the experimental period. Data was analyzed with PROC MIXED in SAS 9.4. Dry matter intake (18.1 ± 0.70 kg), serum mineral concentrations (Cu, Mn, Se, and Zn), and rumen pH (6.5 ± 0.64) did not differ among the treatments. However, serum concentration of Co was higher in 200% ORG compared to 50% and 100% INO and 50% ORG. Ruminal concentration of acetate was higher in 50% and 100% ORG compared to 200% ORG. Butyrate ruminal concentration was higher at 50% ORG compared to 200% ORG. Ruminal propionate concentration was higher in 50% INO and 50% ORG compared to 100% INO and 200% ORG. These findings demonstrate serum trace mineral status and ruminal pH are not tightly controlled by the source of trace minerals when supplemented at 50%, 100% and 200% of the NRC recommendations, however rumen fermentation may be affected by the dietary concentration of trace minerals in the diet.

High Intake of Magnesium in Relation to the Ruminal Transmural Potential Difference and Magnesium Absorption in Wethers

2004 ◽  
Vol 74 (3) ◽  
pp. 217-222
Author(s):  
Jittakhot ◽  
Schonewille ◽  
Haddad ◽  
Wouterse ◽  
Yuangklang ◽  
...  
Keyword(s):  
Dry Matter ◽  
Latin Square ◽  
Potential Difference ◽  
High Intake ◽  
Individual Values ◽  
High Potassium ◽  
High K ◽  
The Mean ◽  
The Individual ◽  
Low K

High potassium (K) intakes are known to decrease magnesium (Mg) absorption in ruminants by increasing the transmural potential difference (PDt, serosal side = positive). High Mg intakes are known to increase the amount of Mg absorbed, which may be explained by increasing the ruminal Mg concentration, but an effect on the PDt cannot be excluded. The objective of this study was to determine whether or not Mg intake affects the PDt. In a 3x3 Latin square design, six ruminally fistulated wethers were fed a low-Mg, low-K ration (3.88 g Mg/kg dry matter (DM); 30.7 g K/kg DM), a high-Mg, low K-ration (16.79 g Mg/kg DM; 30.7 g K/kg DM), and a low-Mg ration high-K (3.88 g Mg/kg DM or 62.1 g K/kg DM). When compared with the low-Mg, low-K ration, the high-Mg, low-K ration raised the absolute apparent Mg absorption (g/day) by 421% and the low-Mg, high-K ration decreased it by 20%. The intake of extra K produced a significant increase in the PDt. The intake of extra Mg did not change the PDt across the rumen wall but produced a significant increase of the ruminal Mg concentrations. On the basis of the individual values for three rations, the mean post feeding ruminal Mg concentrations were found to be unrelated to the PDt (Pearson's r = –0.329, p = 0.183, n = 18). Thus, it is concluded that the observed increase in Mg absorption after a high Mg intake can be explained by an increase in the ruminal Mg concentration rather than by a change in PDt.

A comparison of tall fescue and orchardgrass silages for lactating cows

1993 ◽  
Vol 73 (4) ◽  
pp. 907-914 ◽  
Author(s):  
L. J. Fisher ◽  
S. Bittman ◽  
D. E. Hunt ◽  
J.A. Shelford ◽  
B. D. Mason
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Plasma Urea ◽  
Lactating Cows ◽  
Boot Stage ◽  
Molar Percent ◽  
Preference Trial

The feeding value of tall fescue silage (Festuca arundinacea schreb.) harvested at the boot stage of maturity with a crude protein (CP) content of 15.4% was compared to that of orchardgrass silage (Dactylis glomerata) harvested at the same stage of maturity with a CP content of 12.5%. Two feeding trials with lactating cows were used in this comparison. The first trial employed 10 cows in a 2 × 2 Latin- square, with treatment period 35 d in length. In the second experiment five cows were used in a preference trial where individual feed intakes, duration of feeding, and rate of forage consumption were monitored electronically for a period of 9 d.In the first trial, the cows consumed more tall fescue than orchardgrass silage (P < 0.05) (14.08 vs. 12.90 kg d−1 dry matter (DM)) but there were no differences between cows fed the two silages in milk yield (30.4 vs. 29.8 kg d−1) or milk compositon. Source of silage did not influence rumen fluid pH, molar percent of volatile fatty acids or plasma glucose; however, plasma urea-N levels were significantly higher (P < 0.05) (17.00 vs. 15.14 mg dL−1) when cows were fed tall fescue silage compared with when they were fed orchardgrass silage. In the preference trial employing electronically monitored forage feeding gates, all five cows demonstrated a preference for the tall fescue silage consuming a daily average of 9.83 kg DM for tall fescue compared with 3.73 kg for orchardgrass silage. Cows spent more time (P < 0.05) (68.3 vs. 14.1 min−1) and ate faster (69.6 vs. 54.0 g DM min−1) from the gates offering tall fescue silage compared with the gates offering orchardgrass silage. From the results of these two trials it was concluded that tall fescue silage was comparable to orchardgrass in supporting milk production and superior to orchardgrass in terms of palatability. Key words: Tall fescue, silage preference, lactating cows

scholarly journals AN EVALUATION OF STEAM-TREATED ASPEN AS A SUBSTITUTE FOR CORN SILAGE IN THE RATIONS OF LACTATING COWS

1980 ◽  
Vol 60 (2) ◽  
pp. 379-384
Author(s):  
L. J. FISHER
Keyword(s):  
Acetic Acid ◽  
Milk Yield ◽  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Latin Square ◽  
Corn Silage ◽  
Lactating Cows ◽  
Milk Protein Content ◽  
Forage Mixtures

Fifteen lactating Holsteins were used to test processed aspen added to corn silage at the levels of 0, 10 and 20% (dry matter basis). The experiment was designed as a latin square with three experimental periods each 42 days in length. The forage mixtures were fed free choice to the cows, which were housed in a free-stall barn, and their individual feed intakes were recorded by using electronic doors. The processed aspen contained 45.4% dry matter, 73.7% acid detergent fiber and 0.54% protein. There was no evidence of heating or mold growth in the aspen during the 5 mo of the trial. Silage dry matter intake and milk yield were 11.8, 13.4 and 13.4 and 25.7, 27.0 and 26.8 kg per day, respectively, for the silage mixtures containing 0, 10 and 20% processed aspen. Fat test was decreased slightly and milk protein content significantly (P < 0.05) depressed by the addition of 20% processed aspen chips to the corn silage. Efficiency of milk yield was not significantly influenced (P > 0.05) by the inclusion of aspen chips in the silage. Neither the molar proportion of acetic acid nor the ratio of acetic acid to propionic acid in the rumen fluid was changed when the cows were fed silage containing processed aspen chips. The apparent digestibilities of total ration dry matter and protein were lowered significantly by the addition of 10% aspen chips to the forage mixture. It was concluded from the results of this trial that steam-processed aspen chips had little nutritive value when fed to lactating cows as a partial substitute for corn silage.

scholarly journals Influence of ruminal methane on digesta retention and digestive physiology in non-lactating dairy cattle

2016 ◽  
Vol 116 (5) ◽  
pp. 763-773 ◽  
Author(s):  
Marie T. Dittmann ◽  
Kirsty J. Hammond ◽  
Paul Kirton ◽  
David J. Humphries ◽  
Les A. Crompton ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Positive Association ◽  
Feedback Mechanism ◽  
Negative Association ◽  
Digestive Physiology ◽  
Lactating Cows ◽  
Enteric Methane ◽  
Lactating Dairy Cattle ◽  
Chewing Activity

AbstractEnteric methane (CH4) production is a side-effect of herbivore digestion, but it is unknown whether CH4 itself influences digestive physiology. We investigated the effect of adding CH4 to, or reducing it in, the reticulorumen (RR) in a 4×4 Latin square experiment with rumen-fistulated, non-lactating cows, with four treatments: (i) control, (ii) insufflation of CH4 (iCH4), (iii) N via rumen fistula, (iv) reduction of CH4 via administration of bromochloromethane (BCM). DM intake (DMI), apparent total tract digestibility, digesta mean retention times (MRT), rumen motility and chewing activity, spot breath CH4 emission (CH4exhal, litre/kg DMI) as well as CH4 dissolved in rumen fluid (CH4RRf, µg/ml) were measured. Data were analysed using mixed models, including treatment (or, alternatively, CH4exhal or CH4RRf) and DMI relative to body mass0·85 (rDMI) as covariates. rDMI was the lowest on the BCM treatment. CH4exhal was highest for iCH4 and lowest for BCM treatments, whereas only BCM affected (reduced) CH4RRf. After adjusting for rDMI, CH4RRf had a negative association with MRT in the gastrointestinal tract but not in the RR, and negative associations with fibre digestibility and measures of rumination activity. Adjusting for rDMI, CH4exhal had additionally a negative association with particle MRT in the RR and a positive association with rumen motility. Thus, higher rumen levels of CH4 (CH4exhal or CH4RRf) were associated with shorter MRT and increased motility. These findings are tentatively interpreted as a feedback mechanism in the ruminant digestive tract that aims at mitigating CH4 losses by shortening MRT at higher CH4.

scholarly journals Ruminal parameters and nitrogen balance in sheep fed diets containing residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (5) ◽  
pp. 3411 ◽  
Author(s):  
Luiz Juliano Valério Geron ◽  
Jocilaine Garcia ◽  
Fabiana Gomes Costa ◽  
Sílvia Cristina de Aguiar ◽  
Edimar Barbosa Oliveira ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Experimental Period ◽  
Ammonia Nitrogen ◽  
Linear Increase ◽  
Ruminal Fluid ◽  
Linear Effect ◽  
Mixed Breed ◽  
Ph Level ◽  
Male Sheep

<p>This study measured the ruminal parameters, feed intake, fecal and urinary N production, and N balance (NB) in sheep fed increasing levels of residue from the extraction of tamarind pulp (RETP) on a 0.0%, 5.0%, 10.0%, and 15.0% dry matter (DM) basis. Four mixed-breed male sheep weighing 40.38 ± 2.10 kg of body weight were distributed in a 4 × 4 Latin square design. The animals were allocated to metabolism cages and fed twice a day. Each experimental period lasted 20 days. The pH and concentration of ammonia nitrogen (NH3-N) in ruminal fluid were measured. Nitrogen intake (NI), fecal N (FN), urinary N (UN), absorbed N (AN), and NB were expressed in g·day-1, percentage of consumed nitrogen (CN), and g·kg-1 of metabolic weight (g·(kg0.75)-1. Statistical analysis of these variables was performed by evaluating the variance and regression at 5% probability. The levels of RETP did not alter (p&gt;0.05) the pH level or NH3-N concentration in the rumen fluid, but a quadratic behavior for the same values after feeding (p&lt;0.05) was observed. The inclusion of RETP in the diet did not change (p&gt;0.05) NI, UN, the AN in g·day-1 and g·(kg0.75)-1, NB in g·day-1 and g·(kg0.75)-1, or the percentage of CN. However, the AN as a percentage of CN showed a linear effect (p&lt;0.05) with the inclusion of RETP in experimental diets. For FN in g·day-1 and g·(kg0.75) -1, no difference was observed (p&gt;0.05) with the inclusion of RETP, but for FN expressed as a percentage of CN, a linear increase (p&lt;0.05) was observed with the inclusion of RETP. Thus, we concluded that diets with up to 15.0% RETP do not alter the pH and NH3-N in ruminal fluid, NI, UN, or NB. In addition, the inclusion of 15.0% RETP has a laxative affect. </p>

scholarly journals Ethological activities, ruminal parameters and weight gain of calves supplemented with different amounts of sodium chloride

2012 ◽  
Vol 32 (8) ◽  
pp. 803-806
Author(s):  
Pedro Malafaia ◽  
Rosane Scatamburlo Lizieire ◽  
Paula Barroso Cruz ◽  
Paulo Vargas Peixoto
Keyword(s):  
Weight Gain ◽  
Sodium Chloride ◽  
Water Consumption ◽  
Rumen Fluid ◽  
Average Daily Gain ◽  
Daily Intake ◽  
Experimental Period ◽  
Daily Weight Gain ◽  
Rumen Protozoa ◽  
Significant Difference

The daily weight gain, behavioral activities (grazing, ruminating and water consumption) and the number of rumen protozoa, pH, NH3, and the osmolarity of rumen fluid was evaluated for four groups of six calves HPB/Zebu submitted to daily intake of 15, 30, 60 and 90g of NaCl during 135 days (9th Jan. to 24th May 2008).Throughout the experimental period the calves were in perfect health condition and did not show any signs suggestive of chronic sodium poisoning. There was no significant difference in average daily gain of the calves as the intake of NaCl increased. No significant behavioral changes were observed in the activities of grazing and rumination. However, there was a noticeable change in the frequency of water consumption in the calves that ingested 90g/d of NaCl; they went more often to the trough and drank more water than the group that ingested only 15g sodium chloride/d. The osmolarity of ruminal liquid was higher in the group of calves fed 90g of NaCl/d. The pH, NH3 concentration and number of rumen protozoa was within the normal range.

scholarly journals Responses in the voluntary intake of hay or silage by lactating cows to intraruminal infusions of sodium acetate or sodium propionate, the tonicity of rumen fluid or rumen distension

1993 ◽  
Vol 69 (3) ◽  
pp. 699-712 ◽  
Author(s):  
M. H. Anil ◽  
J. N. Mbanya ◽  
H. W. Symonds ◽  
J. M. Forbes
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Sodium Propionate ◽  
Lactating Cows ◽  
Rubber Balloon ◽  
Balloon Distension ◽  
Voluntary Intake ◽  
Dose Dependent

Rumen-fistulated lactating cows were individually fed on hay or silage and intakes were monitored during 3 h treatment periods and for 2 h after. Each experiment used five, six or seven animals and the treatments were applied in a Latin Square design. Sodium acetate infusions of 1·8–11·0 mol in 4.5 litres water caused a dose-related depression in hay intake, the extent being 82 g dry matter (DM)/mol infused (P < 0·01). Sodium acetate infusions of 6·0–15·0 mol in 4·5 litres water caused a dose-related depression in silage intake of 118 g DM/mol infused. Rumen fluid pH for both diets was unaffected by treatment. Acetate and Na concentrations were increased and significantly negatively correlated with intake of both diets. Infusions of 2–8 mol sodium propionate caused a dose-related depression of hay intake which was significant when cow and day effects were accounted for. Sodium propionate infusions of 4–8 mol significantly depressed silage intake by 140 g DM/mol infused (P < 0·001). Rumen fluid pH was unaffected by treatment while propionate and Na concentrations were elevated and significantly negatively correlated with intake for both diets. Inflation of a rubber balloon in the rumen with 12.5–20 litres warm water resulted in a dose-dependent depression in hay intake of 66 g DM/1 distension (P < 0·05). There was significant overeating during the 2 h following the 20 litre treatment. With silage, 15–25 litres of balloon distension for 3 h resulted in a dose-dependent depression in intake of 28 g DM/l distension (P < 0·001). There was no significant overeating during the 2 h following distension. When given in physiological amounts, at the lower end of the range used in these experiments, acetate, propionate and distension of the rumen did not significantly affect hay intakes. However, in each case the linear relationship between intake depression and level of treatment suggested that these factors could contribute to the control of feed intake.

Evaluation of rumen probe for continuous monitoring of rumen pH, temperature and pressure

2010 ◽  
Vol 50 (2) ◽  
pp. 98 ◽  
Author(s):  
R. Kaur ◽  
S. C. Garcia ◽  
A. Horadagoda ◽  
W. J. Fulkerson
Keyword(s):  
Correlation Coefficient ◽  
Rumen Fluid ◽  
Latin Square ◽  
Sampling Period ◽  
Experimental Period ◽  
Primary Objective ◽  
Concordance Correlation ◽  
Rumen Ph ◽  
Temperature And Pressure ◽  
A Minor

The primary objective of this study was to evaluate the accuracy of a commercially available wireless rumen probe by Kahne Limited (New Zealand) for continuous pH, temperature and pressure measurements under different ruminal conditions. In a 4 by 4 latin square design, rumen-fistulated sheep were fed diets comprising 0, 30 or 60% concentrate, with the rest of the diet being balanced for metabolisable energy and protein with maize silage and lucerne hay. Each experimental period was 10 days with the first 8 days for adaptation and the last 2 days for collection of rumen fluid samples. In the first experimental period, probes were left in the rumen of sheep for 10 days to observe drift in pH. In the other three periods, probes were repeatedly cleaned and recalibrated before each sampling period. Probes were set to read every 20 min while the samples of rumen fluid were withdrawn manually at 4-h intervals and pH recorded immediately. There was an upward drift in pH observed after 48 h of insertion of probes into the rumen. This study resulted in a minor level of agreement between the two methods as indicated by higher root mean prediction error (0.43 pH units), lower Pearson’s correlation coefficient (r = 0.46) and concordance correlation coefficient (0.46). In conclusion, these rumen probes need further advancement to be potentially used for continuous rumen pH measurements for research purposes.

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