Effect of dietary potassium on concentration of lysine in plasma of finishing cattle

1992 ◽  
Vol 72 (3) ◽  
pp. 603-611
Author(s):  
G. L. Newton ◽  
G. M. Hill ◽  
R. W. Beaver ◽  
B. G. Mullinix Jr.

High K intakes have been shown to affect lysine metabolism in swine and poultry. This effect may result in improved performance and its primary manifestation is a decrease in plasma and tissue lysine concentration. In order to evaluate the effects of supplemental K and crude protein (CP) on the concentration of amino acids in plasma (PLAA) and growth performance, 72 cattle (48 heifers + 24 steers, weighing 425 ± 35 kg) were allotted to a 2 × 2 factorial arrangement of dietary treatments in a randomized complete block design trial. After 28 d on feed, there was a trend (P = 0.09) for an interaction effect of K (1% KCl) and CP (6% cottonseed meal) on plasma lysine. At 56 d there was an interaction (P = 0.02) of K and CP on plasma lysine. Supplemental K decreased (P = 0.01) plasma lysine in the absence of CP supplement, and increased it (P = 0.04) in the presence of CP. Supplemental CP decreased (P = 0.001) plasma lysine in the absence of supplemental K, but not in its presence. Addition of K, CP or both K and CP to the corn-peanut hull diet did not affect weight gain or feed efficiency. Addition of CP tended (P = 0.09) to increase feed intake over the entire trial. Carcass quality grade was lower (P = 0.03) for cattle fed CP supplement than for cattle not fed supplemental CP. Key words: Cattle, potassium, crude protein, plasma amino acids, lysine

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 351-352
Author(s):  
S-M Hong ◽  
D Jung ◽  
S-J You

Abstract A body of evidence suggests that low-protein diets in swine have benefits in view of environmental stewardship and economic returns. The present study was performed to evaluate essential amino acid (EAA) supplementation in the diets with reducing crude protein (CP) concentrations on growth performance in fattening pigs. We hypothesized that a low-CP diet with elevated EAA concentrations would result in similar growth performance in fattening pigs compared with a normal CP diet. Eighty finishing pigs with initial body weight (BW) of 72.0 ± 5.34 kg were assigned in a randomized complete block design with 5 replicates to test 4 dietary treatments: 1) 15.5% CP with EAA concentration at 109% of NRC requirement (control); 2) 14.0% CP with EAA concentration at 111% of NRC requirement (EAA111); 3) 14.0% CP with EAA concentration at 113% of NRC requirement (EAA113); and 14.0% CP with EAA concentration at 115% of NRC requirement (EAA115). The diets were isocaloric, whereas crystalline amino acids, L-Lys, L-Met, L-Thr, L-Trp, and L-Val were added to the diets to achieve the varying levels of EAA in the treatments. The Lys-to-net energy ratio in the diets was maintained at 3.06, 3.12, 3.18, and 3.24 to the control, EAA111, EAA113, and EAA115, respectively. Dietary treatments did not affect final BW. Additionally, average daily gain, average daily feed intake, and feed conversion ratio (FCR) were similar across the treatments. On average, pigs fed the control and EAA111 numerically decreased FCR compared to those fed EAA113 and 115 (3.21 vs. 3.35, respectively; P = 0.46). Total feed cost throughout the experiment was $9.0/head lessen in EAA111 relative to the control; however, total feed costs to EAA113 and EAA115 were greater than the control. In summary, a low-CP diet with increased EAA supplementations did not have any negative impact on growth performance of fattening pigs.


2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Minoy Cristobal ◽  
Jessica P Acosta ◽  
Su A Lee ◽  
Hans H Stein

Abstract AbstractThree experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of amino acids (AA), concentration of metabolizable (ME), and standardized total tract digestibility (STTD) of P in a new source of distillers dried grains with solubles (DDGS; ProCap DDGS) are greater than in conventional de-oiled DDGS. In experiment 1, nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicated 3 × 3 Latin square design with three diets and three periods for a total of nine replicate pigs per diet. Two diets included ProCap DDGS or de-oiled DDGS as the sole source of crude protein (CP) and AA. An N-free diet was used to determine the basal endogenous losses of CP and AA. Ileal digesta were collected on days 5 and 6 of each period after 4 d of adaptation to diets. Results from experiment 1 indicated that ProCap DDGS contained more CP and AA compared with de-oiled DDGS. The SID of all AA in ProCap DDGS was greater (P < 0.001) compared with de-oiled DDGS with the exception that the SID of Pro was not different between the two sources of DDGS. In experiment 2, 24 growing barrows (initial BW: 32.7 ± 3.1 kg) were housed individually in metabolism crates and used in a randomized complete block design and fed a corn-based diet or two diets containing corn and each source of DDGS with eight replicate pigs per diet. Fecal and urine samples were collected for 4 d after 7 d of adaptation. Results from experiment 2 indicated that concentration of ME in ProCap DDGS was greater (P < 0.05) compared with corn or de-oiled DDGS. In experiment 3, 32 growing barrows (initial BW: 20.2 ± 0.9 kg) were placed in metabolism crates and allotted to four diets with eight pigs per diet using a 2 × 2 factorial treatment arrangement. The de-oiled DDGS and ProCap DDGS were both included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Pigs were adapted to the diets for 5 d and fecal samples were collected for 4 d. Results from experiment 3 indicated that inclusion of phytase in the diet containing ProCap DDGS increased (P < 0.05) the STTD of P, but addition of phytase to the de-oiled DDGS diet did not increase STTD of P (interaction, P < 0.001), but the STTD of P was greater (P < 0.05) in de-oiled DDGS compared with ProCap DDGS. In conclusion, ProCap DDGS has greater SID of AA and contains more ME, but has reduced STTD of P compared with conventional de-oiled DDGS.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 61-61
Author(s):  
Chan Sol Park ◽  
Ayodeji S Aderibigbe ◽  
Gary Hayen ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to evaluate the digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in dried yeast (DY) and soybean meal (SBM) fed to pigs. In Exp. 1, 30 barrows with an initial body weight (BW) of 20.7 ± 1.01 were assigned to 5 diets in a randomized complete block design with period and BW as blocking factors. A basal diet was prepared to contain corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5 or 10 g/kg DY or SBM at the expense of energy-contributing ingredients in the basal diet to estimate the DE and ME in test ingredients by regression analysis. On a dry matter basis, estimated DE and ME in DY were 4,022 and 3,352 kcal/kg, respectively, and those in SBM were 3,876 and 3,601kcal/kg, respectively. There was no difference in estimated DE or ME between DY and SBM. In Exp. 2, 21 barrows (initial BW = 20.0 ± 1.31 kg) surgically fitted with T-cannulas at the distal ileum were assigned to 3 diets in a randomized complete block design with BW as a blocking factor. Two diets were prepared to contain DY or SBM as the sole source of nitrogen, and a nitrogen-free diet was prepared to determine the basal ileal endogenous losses of AA. The SID of AA, except for Gly and Pro, in SBM were greater (P < 0.05) than in DY. The SID of indispensable AA in DY ranged from 64.7% for Thr to 86.1% for Arg, whereas those in SBM ranged from 84.8% for Thr to 92.3% for Arg. In conclusion, energy values in DY was comparable with SBM, but the SID of most AA in DY were less than in SBM.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 118-118
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim

Abstract The objective was to evaluate Yarrowia lipolytica (YL) as a lipid supplement fed to nursery pigs for the growth performance and apparent ileal digestibility (AID). Twenty-four pigs weaned at 21 d of age (12 barrows and 12 gilts with initial BW at 7.2 ± 0.6 kg) were allotted to 3 dietary treatments (n = 8) based on the randomized complete block design with sex and BW as blocks. The main effect was the dietary supplementation of YL (0, 1.5, and 3.0%, replacing poultry fat) with nutrients meeting or exceeding the requirements by NRC (2012) and fed to pigs for 21 d based on 2 phases. Feed intake and BW were recorded at d 0, 10, and 21 to calculate ADG, ADFI, and G:F. Fecal scores were recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect ileal digesta for AID of nutrients in diets. Data were analyzed using Proc Mixed of SAS. There were no differences in growth performance among treatments. Whereas, pigs fed 0 and 1.5% YL had higher (P < 0.05) AID of DM (55.0 and 52.2% to 40.2%, respectively) and GE (62.1 and 60.6% to 49.8%, respectively) than pigs fed 3% YL. There were no differences in AID of DM and GE between the pigs fed 0% and 1.5% YL. In conclusion, 1.5% Yarrowia lipolytica could be supplemented in nursery pig diets reducing 0.8% of poultry fat without affecting growth performance and AID of DM and GE.


2020 ◽  
Vol 45 (2) ◽  
pp. 256-264
Author(s):  
T. F. Mbahi ◽  
S. Maidadi ◽  
Z. A. Gworgwor ◽  
Y. Danladi

An experiment was conducted to evaluate the effect of growth, biomas yieds and nutritive value of Lablab purpureus, Centrosema pubescens and Mucuna pruriens under rain fed condition in Yola, Adamawa State, Nigeria. The field experiment was laid in a randomized complete block design with the plot divided into three main plots and replicated three times measuring 5x5m with inter and intra row spacings of 0.5m. The five harvesting stages are 6, 8, 10, 12 and 14 weeks, respectively. The growth in height increases with stage of growth and declined with reduction in rainfall. Higher growth were recorded in T3 (3.8 -354.8cm) followed by T2 (2.96-260.20cm) and then T1 (1.50-213.00cm) and the results were presented graphically. The result of the chemical composition of all the legumes showed that the crude protein decreases with stage of growth from weeks 6 -14 (14.40, 12.26, 10.65-10.08, 6.79, 6.49) and ADF and NDF (25.20, 32.15, 27.35-42.13, 40.33, 36.50 and 32.20, 54.45, 47.00- 51.24, 63.53, 66.33), respectively. The biomass yields were significantly different (P<0.05) for all the legumes at different harvesting stages T1 (2744.53-3186.93), T2 (2696.47- 3006.70) and T3 (2843.73-3147.63). It is therefore concluded that legumes could best be harvested between weeks 10 and 12 when the yield and quality are at levels to sustain the animals both for maintenance and production.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 112-112
Author(s):  
Richard B Omidiwura ◽  
Adebisi F Agboola ◽  
Taiwo O Makinde ◽  
Grace O Oyebode

Abstract An experiment was conducted to investigate the effect of rice bran (RB) and Ronozyme® WX on apparent and true ileal amino acid (AA) digestibility coefficients in broiler chicks using a regression approach. Two hundred and forty 21-day-old Abor Acre plus chicks were randomly allotted to six diets with varying levels of RB (100, 200 and 300 g/kg) without (0g/kg) or with Ronozyme® WX (25g/kg) supplementation in a 2x3 factorial arrangement using a randomized complete block design. Each treatment had 5 replicates with 8 birds each. The birds were fed for 5 days (d 22–26). On d 26, digesta was sampled from distal ileum. Data were analyzed using general linear model at α0.05. Lysine had the highest concentration among the essential amino acids (AAs) while the lowest concentration was observed for threonine. Rice bran at 10% for the essential amino acids with enzyme was significantly higher but compared with concentrations of RB at 10% and 20% without enzyme. There were no significant differences among the true ileal amino acid digestibility coefficients except lysine (10% RB) without enzyme (0.91) which was significantly higher than 20% RB with enzyme (0.84) but similar to other treatments. Rice bran with enzyme had a significant (P &lt; 0.05) interactive effect on the apparent ileal digestibility coefficients of arginine, histidine, isoleucine methionine and phenylalanine. When RB was supplemented with enzyme, a significant (P &lt; 0.05) interactive effect on true ileal digestibility coefficients of arginine and methionine was observed. Rice bran-enzyme interaction had no significant effect on the non-essential AAs. The slopes of the regression lines ranged from 0.77 (arginine) to 0.98 (phenylalanine). In conclusion, enzyme supplementation did not significantly influence the digestibility coefficients of AA in rice bran. However, the inclusion of 10% and 20% RB supplemented with Ronozyme® can help to improve the methionine content in rice bran.


2019 ◽  
Vol 4 (1) ◽  
pp. 75-83
Author(s):  
Caitlin Swedzinski ◽  
Kelly A Froehlich ◽  
Karim W Abdelsalam ◽  
Christopher Chase ◽  
Tony J Greenfield ◽  
...  

Abstract A blend of essential oils (EO) and a prebiotic were combined (EOC) to formulate a colostrum-based liquid birth supplement and a separate feeding supplement (Start Strong and Stay Strong, Ralco Inc., Marshall, MN). These products were designed to promote immunity and stimulate appetite to diminish health challenges and stresses experienced by newborn calves. The hypothesis was that calves supplemented with an oral dose of liquid EOC at birth (10-mL aliquot at birth and 10 mL at 12 h of age) when fed the EOC feeding supplement would result in improved growth performance, health, and immunity. The objective was to determine if an additional feeding of liquid EOC at birth in combination with EOC in the milk replacer (MR) would allow calves to demonstrate improved growth, health, and immunity compare with calves only offered EO in MR. Sixty-one Holstein calves (18 males and 43 females) from a commercial dairy operation were blocked by birth date and randomly assigned to 1 of 3 treatments. Treatments were 1) Control (CON): a 24% crude protein (CP):20% fat (as-fed basis) MR; 2) EP: a 24:20 MR with EOC mixed at 1.25 g/d; or 3) EPC: a 24:20 MR with EOC mixed at 1.25 g/d in addition to calves receiving one 10-mL oral dose of liquid EOC at birth and 10 mL again at 12 h. The 24:20 MR was fed via bucket 2 times per day at a rate of 0.57 kg/calf daily for 14 d, increased to 0.85 kg/calf at 2 times per day until 35 d and was reduced to 0.43 kg at 1 time per day at 36 d to facilitate weaning after 42 d. Decoquinate was added to the MR at 41.6 mg/kg for coccidiosis control. Calves were housed in individual hutches bedded with straw with ad libitum access to a 20% CP-pelleted calf starter and water. All data were analyzed using PROC MIXED as a randomized complete block design. Calves in this study had similar (P &gt; 0.10) average daily gains, body weight, and growth measurements. Calves fed EPC had significantly (P &lt; 0.05) higher IgA titers on day 0 of the trial compared with calves fed EP or CON, which was expected as calves were supplemented with liquid EOC at birth and 12 h later demonstrating an increase in immune response. The use of a liquid EOC product being administrated after birth can improve IgA titers to improve the immune status of the new born calf to fight off potential diseases and pathogens. A formulation error resulted in the EOC being fed at half the rate of the previous experiment of 2.5 g/d, which appears to be below an efficacious dosage.


2019 ◽  
Vol 97 (10) ◽  
pp. 4032-4040
Author(s):  
Carine M Vier ◽  
Steve S Dritz ◽  
Fangzhou Wu ◽  
Mike D Tokach ◽  
Joel M DeRouchey ◽  
...  

Abstract Two experiments were conducted to determine the standardized total tract digestible phosphorus (STTD P) requirement for 11- to 23-kg nursery pigs fed diets with or without phytase. A total of 1,080 and 2,140 pigs (PIC 359 × Camborough, Hendersonville, TN; initially 11.4 ± 0.29 and 11.1 ± 0.24 kg) were used in Exp. 1 and Exp. 2, respectively. There were 23 to 27 pigs per pen with 6 and 12 replicate pens per treatment in Exp. 1 and Exp. 2, respectively. After weaning, pigs were fed a common pelleted diet with 0.45% STTD P for 7 d, and a common phase 2 meal diet with 0.40% STTD P for 14 d in Exp. 1 and 18 d in Exp. 2. Pens of pigs were then allotted to dietary treatments in a randomized complete block design with body weight (BW) as the blocking factor. In Exp. 1, dietary treatments consisted of 0.26%, 0.30%, 0.33%, 0.38%, 0.43%, 0.48%, and 0.53% STTD P. Treatments were achieved with the inclusion of monocalcium phosphate at the expense of corn. In Exp. 2, diets contained 1,000 phytase units (FYT; Ronozyme Hiphos 2500, DSM Nutritional Products, Inc., Parsippany, NJ) with assumed release value 0.132% STTD P, and treatments consisted of 0.30%, 0.33%, 0.38%, 0.43%, 0.48%, 0.53%, and 0.58% STTD P. These STTD P concentrations included the expected phytase release of 0.132% STTD P. In both experiments, a similar 1.17:1 Ca:P ratio was maintained across treatments. Statistical models included linear model (LM), quadratic polynomial (QP), broken-line linear (BLL), and broken-line quadratic (BLQ). In Exp. 1, increasing STTD P increased (linear, P < 0.001) ADG, ADFI, G:F, final BW, and grams of STTD P intake per day and per kilogram of gain. There was also a marginal quadratic response for G:F (P < 0.066). In Exp. 2, ADG and G:F increased quadratically (P < 0.05), whereas ADFI increased linearly (P = 0.060) with increasing STTD P. The BLL and QP model provided similar fit to G:F in Exp. 1, estimating the requirement for maximum G:F at 0.34% and 0.42%, respectively. The BLL was the best fitting model for ADG and G:F in Exp. 2, estimating the breakpoint at 0.40% and 0.37% STTD P, respectively. The BLL and BLQ models estimated the breakpoint for ADG as a function of STTD P intake in grams per day at 2.92 and 3.02 g/d, respectively. These data provide empirical evidence that for 11- to 23-kg pigs, the NRC (2012) accurately estimates the STTD P requirement on a g/d basis. As a percentage of the diet, the STTD P requirement for diets without or with 1,000 FYT added phytase ranged from 0.34% to 0.42%.


2020 ◽  
Vol 4 (2) ◽  
pp. 1140-1147
Author(s):  
Jerad R Jaborek ◽  
Henry N Zerby ◽  
Macdonald P Wick ◽  
Francis L Fluharty ◽  
Steven J Moeller

Abstract The effects of dietary energy source, energy level, sheep age, and sheep sex on flavor and off-flavor intensity were evaluated. Consumer panelists, with previous lamb-eating experience, assessed lamb flavor and off-flavor intensity on a 100-point, end-anchored scale (0 = very mild to 100 = very intense), with off-flavor being defined as anything different than lamb flavor. Lamb longissimus thoracis (LT) and whole, boneless ground shoulder (GS) formed into patties were evaluated. Trial 1 was a randomized complete block design with a 3 × 2 factorial arrangement of treatments. Sheep age (ewe lambs, yearling ewes, and mature ewes; n = 16/age) and ad libitum access to diets [alfalfa pellets (AP) or whole-shelled corn (WSC100)] were treatments. The LT from mature ewes had a greater (P ≤ 0.02) off-flavor intensity when compared with yearling ewes and ewe lambs. Ground shoulder from sheep raised on AP had a greater lamb flavor (P ≤ 0.04) and off-flavor (P ≤ 0.04) intensity than GS from sheep consuming WSC100. Trial 2 was a randomized complete block design with a 3 × 2 × 2 factorial arrangement of treatments. Three dietary treatments [AP, WSC100, and restricted intake of whole-shelled corn to 85% of ad libitum (WSC85)], lamb sex (ewes and wethers; n = 48/sex), and lamb age [short fed, 177 ± 16.6 d of age and 93 ± 20.5 d on feed (DOF); long fed, 294 ± 7.0 d of age and 219 ± 3.8 DOF]. Flavor intensity of the LT was greater (P ≤ 0.05) from lambs offered AP when compared with lambs offered WSC85, whereas lamb flavor of the LT from lambs fed WSC100 was intermediate and not different from the lamb flavor of the LT of lambs fed AP or WSC85. The LT of long-fed lambs had a greater (P ≤ 0.01) lamb flavor and off-flavor intensity when compared with short-fed lambs. Lambs offered AP resulted in a GS with greater lamb flavor intensity (P ≤ 0.01) when compared with lambs offered WSC85 and WSC100, with no diet influence on GS off-flavor intensity. Long-fed lambs produced GS with a greater lamb flavor (P ≤ 0.01) and off-flavor (P ≤ 0.01) intensity when compared with GS from short-fed lambs. Results from the two trials indicate lamb flavor and off-flavor intensity were greater from sheep offered a high-forage (AP) diet when compared with a high-concentrate (WSC) diet. Lamb flavor intensity increased as age of the sheep at harvest increased, suggesting dietary management and associated age-related effects at harvest will influence consumer perception of lamb flavor.


2018 ◽  
Vol 53 (2) ◽  
pp. 221-228
Author(s):  
Gil Mario Ferreira Gomes ◽  
Magno José Duarte Cândido ◽  
Marcos Neves Lopes ◽  
Theyson Duarte Maranhão ◽  
Dhones Rodrigues de Andrade ◽  
...  

Abstract: The objective of this work was to evaluate the effects of combined phosphorus and nitrogen fertilization and of harvesting frequency on the chemical composition of 'Gigante' cactus pear (Opuntia ficus-indica) cladodes. The experiment was carried out in two municipalities, Quixadá and Tejuçuoca, in the state of Ceará, Brazil. Fertilization with nitrogen (urea) and phosphorus (single superphosphate) was done with nine doses, respectively: 10 and 70, 70 and 10, 70 and 70, 70 and 130, 100 and 100, 130 and 70, 130 and 130, 130 and 190, and 190 and 130 kg ha-1 per year, besides 100 kg ha-1 N and P2O5 per year as the control. The harvesting frequencies evaluated were annual and biannual. A randomized complete block design was adopted, in split plot, with four replicates. The effect of fertilization on the contents of total dry matter, crude protein, neutral detergent fiber, organic matter, and total carbohydrates in the cladodes varies with harvesting frequency and site. Regarding harvesting frequency, there were higher contents of organic matter, total carbohydrates, and neutral detergent fiber, but lower contents of crude protein for the biannual harvesting in both municipalities. Annual harvesting improves the nutritional quality of primary and secondary cladodes in the municipality of Quixadá and of secondary and tertiary cladodes in Tejuçuoca.


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