Effects of probiotic supplementation of hull-less barley- and corn-based diets on bacterial fermentation in continuous culture of ruminal contents

1992 ◽  
Vol 72 (2) ◽  
pp. 265-272 ◽  
Author(s):  
P. M. Windschitl
Keyword(s):  
Continuous Culture ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ruminal Fermentation ◽  
Probiotic Supplementation ◽  
Yeast Saccharomyces Cerevisiae ◽  
Bacterial Fermentation ◽  
Dry Matter Digestibility ◽  
Alfalfa Meal ◽  
Carbohydrate Digestibility

A study was conducted using a continuous culture fermentation system to determine effects of probiotic supplementation on ruminal bacterial fermentation of hull-less barley- and corn-based diets. The probiotic contained both a fungal (Aspergillus oryzae) and yeast (Saccharomyces cerevisiae) culture along with several bacterial cultures. Treatments were arranged in a 2 × 2 factorial design with two sources of grain (hull-less barley, B and corn, C) with (+) or without (−) probiotic supplementation. Probiotic was added directly into the fermenter flasks. Diets consisted of (dry matter basis) 46% bromegrass silage, 5% alfalfa meal, and 49% barley- or corn-based concentrate mix. Probiotic supplementation decreased (P < 0.05) dry matter digestibility with the corn diet (C −, 58.6%; C +, 51.4%) but had no significant effect on the barley diet (B −, 48.7%; B +, 51.8%) Dry matter digestibility tended to be higher (P = 0.07) with corn- vs. barley-based diets. Protein degradation and fiber digestibility were not significantly (P > 0.05) affected by probiotic supplementation. Non-fiber carbohydrate digestibility was decreased (P < 0.05) with C + and tended to increase (P = 0.09) with B + compared to C − and B −, respectively (B −, 60.8%; B +, 67.1%; C −, 72.3%; C +, 63.4%). Probiotic supplementation had no significant (P > 0.05) effect on total or individual volatile fatty acids. Although limited, data suggest that type of grain used in the diet can influence the effectiveness of probiotics in altering ruminal metabolism. Key words: Continuous culture, corn, hull-less barley, probiotic, ruminal fermentation

scholarly journals Dietary rambutan peel powder as a rumen modifier in beef cattle

2020 ◽  
Vol 33 (5) ◽  
pp. 763-769
Author(s):  
Thiwakorn Ampapon ◽  
Metha Wanapat
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Napier Grass ◽  
Feed Consumption ◽  
Ruminal Fermentation ◽  
Fruit Peel ◽  
Negative Effect

Objective: The experiment was conducted to study the effect of rambutan (Nephelium lappaceum) fruit peel powder (RP) on feed consumption, digestibility of nutrients, ruminal fermentation dynamics and microbial population in Thai breed cattle.Methods: Four, 2-year old (250±15 kg) beef bull crossbreds (75% Brahman×25% local breed) were allotted to experimental treatments using a 4×4 Latin square design. Four dietary supplementation treatments were imposed; non-supplementation (control, T1); supplementation of RP fed at 2% of dry matter intake (DMI) (low, T2); supplementation of RP fed at 4% of DMI (medium, T3) and supplementation of RP fed at 6% of DMI (high, T4). All cattle were given a concentrate supplement at 1% of body weight while Napier grass was provided as a free choice.Results: The findings revealed that RP supplementation did not negatively affect (p>0.05) DMI of Napier grass, while RP intake and total DMI were the greatest in the RP supplementation at 4% and 6% DMI. Nevertheless, the nutrients (dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) digestibilities were not changed in the RP supplementation groups. Rumen fermentation parameters especially those of total volatile fatty acids, acetate and butyrate were not significantly changed. However, the propionate concentration was remarkably increased (p<0.05) in the RP supplementation. Notably, the ratio of acetate to propionate, the number of protozoa, as well as the methane estimation were significantly reduced in the RP supplemented groups (4% and 6% of DMI), while the counts of bacteria was not altered.Conclusion: Supplementation of RP (4% of DMI) improved rumen propionate production, reduced protozoal population and methane estimation (p<0.05) without a negative effect on feed consumption and nutrients total tract digestibilities in beef cattle. Using dietary rambutan fruit peel powder has potential promise as a rumen regulator.

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

Effects of cinnamaldehyde, eugenol, and capsicum on fermentation of a corn-based dairy ration in continuous culture

2010 ◽  
Vol 90 (3) ◽  
pp. 413-420 ◽  
Author(s):  
L R Tager ◽  
K M Krause
Keyword(s):  
Continuous Culture ◽  
Dry Matter ◽  
Area Under The Curve ◽  
Protein Digestibility ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Fatty Acid Production ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Continuous Culture System

A 12-unit continuous culture system was used in a complete randomized design to study the effects of no oil (CON), cinnamaldehyde oil (CIN), eugenol oil (EUG), and capsicum oil (CAP) (500 mg L-1 d -1) with a 45:55 forage:concentrate ratio (dry matter basis) ration on rumen fermentation. Dry matter digestibility did not differ among treatments. Organic matter digestibility tended to decrease with CIN. Digestibility of neutral detergent and acid detergent fiber tended to be highest with CAP. Crude protein digestibility and bacterial nitrogen flow was depressed with CIN and EUG. CIN tended to decrease microbial protein synthesis and increase effluent ammonia nitrogen. Total volatile fatty acid production did not differ among treatments; however, isovalerate production tended to be highest with CAP. CIN and EUG had higher mean pH, spent fewer hours per day and had smaller area under the curve at pH < 5.6 and 5.8. CAP had smaller area under the curve at pH < 5.6. Supplementation with these oils at the current dose had limited effects on rumen fermentation, with the majority of effects observed being mainly attributable to the very high dosage of oil used. Key words: Dairy cow, essential oil, continuous culture, rumen fermentation, rumen pH

scholarly journals In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution

2020 ◽  
Vol 24 (1) ◽  
pp. 50
Author(s):  
Rusli Fidriyanto ◽  
Roni Ridwan ◽  
Wulansih Dwi Astuti ◽  
Rohmatussolihat Rohmatussolihat ◽  
Nurul Fitri Sari ◽  
...  
Keyword(s):  
Organic Matter ◽  
Rice Husk ◽  
Rice Bran ◽  
Dry Matter ◽  
Acid Level ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Fatty Acid Production ◽  
Dry Matter Digestibility

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.

COMPARATIVE PERFORMANCE AND DIGESTIBILITY IN DAIRY CALVES WEANED AT THREE, FIVE, AND SEVEN WEEKS OF AGE

1985 ◽  
Vol 65 (2) ◽  
pp. 445-450 ◽  
Author(s):  
K. A. WINTER
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Age Groups ◽  
Feed Consumption ◽  
Comparative Performance ◽  
N Retention ◽  
Dry Matter Digestibility ◽  
Bull Calves ◽  
Weaning Age

Ayrshire and Holstein bull calves were weaned onto dry feed at 3, 5, and 7 wk of age. Growth rates from birth to 14 wk of age, and feed intake and feed efficiency from 9 to 14 wk were not affected by weaning age. Dry matter digestibility in the week after weaning was similar for the different weaning age treatments and for the several digestion periods up to 14 wk of age. Nitrogen (N) retention was lower in the 3-wk weaned group immediately after weaning but when compared at uniform ages of 8 and 14 wk N retention was the same in the three weaning age groups. Total volatile fatty acids (VFA) in rumen fluid and proportions of acetic, propionic, and butyric acids were not affected by weaning age in the week after weaning and showed little change in proportions of individual VFA up to 14 wk, when the diet composition was constant. It was concluded that early weaning, at 3 wk of age, had little or no effect on the calf except for a slight slowing in growth during the week after weaning. Dry feed consumption increased rapidly and a relatively stable rumen fermentation developed as quickly in the calves weaned at 3 wk as in those weaned at 5 and 7 wk. In the week after weaning, dry matter and energy were digested as effectively in the early-weaned calves as in those weaned at 5 and 7 wk. Key words: Calves, weaning, digestibility, VFA

THE INTAKE AND DIGESTIBILITY OF AMMONIATED CEREAL STRAWS BY CATTLE

1978 ◽  
Vol 58 (3) ◽  
pp. 471-478 ◽  
Author(s):  
G. M. J. HORTON
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Latin Square ◽  
Crude Fiber ◽  
Plasma Urea ◽  
Ambient Temperatures ◽  
Ruminal Ph ◽  
Dry Matter Digestibility ◽  
Oat Straw

The effects of treatment with 3.5% anhydrous ammonia on the nutritive value of straws from Neepawa wheat, Bonanza barley and Random oats were investigated with growing Hereford steers. Ambient temperatures during the 6-wk post-treatment period were 0.1 °C for wheat and barley straws and 15 °C for oat straw. The four treatments were untreated and ammoniated straw, fed with or without 4-kg supplements per day over four periods to four steers in a 4 × 4 latin square design. Crude protein was doubled in wheat and barley straws and trebled in oat straw following ammoniation. The consumption of all three straws without concentrates was increased significantly by ammoniation. Apparent digestibility of dry matter and crude fiber on straw rations were higher (P < 0.05) in ammoniated wheat and oat straw than in the corresponding untreated straws. Neither straw intake nor dry matter digestibility were improved (P > 0.05) by ammoniation when supplements were fed, though crude fiber digestibility was increased (P < 0.05) in wheat and oat straw rations. Straw treatment had no effect (P > 0.05) on ruminal pH, dehydrogenase activity, or total or molar proportions of volatile fatty acids. Ruminal ammonia concentrations were higher (P < 0.01) for ammoniated than for untreated straws fed alone and similar to those when concentrates were also fed. Plasma urea, measured 0, 0.5, 1, 2, 4 and 7 h post-prandial, was highest in steers fed ammoniated wheat and oat straw and lowest when untreated straw was fed alone.

Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

Comparison of enantiomers of organic acids for their effects on methane production in vitro

2014 ◽  
Vol 54 (9) ◽  
pp. 1345 ◽  
Author(s):  
L. G. Reis ◽  
A. V. Chaves ◽  
S. R. O. Williams ◽  
P. J. Moate
Keyword(s):  
Organic Acids ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Concentration ◽  
The Other ◽  
In Vitro Fermentation ◽  
Ruminal Microorganisms ◽  
Dry Matter Digestibility ◽  
Ch4 Production

This study aimed to evaluate the effect of organic acids on in vitro fermentation characteristics. Four organic acids (tartaric, malic, fumaric and citric) and their enantiomers (L-tartaric, D-tartaric, DL-tartaric, L-malic and DL-malic) were analysed using in vitro batch culture incubations, at four concentrations (0, 5, 10 and 15 mM). Cumulative total gas and methane (CH4) production (mL/g DM) were measured at 6, 12 and 24 h; ammonia, pH, volatile fatty acids (VFA) and in vitro dry matter digestibility (IVDMD) were determined after 24 h of fermentation. Overall, addition of acids at 5 to 15 mM increased (P < 0.0001) cumulative gas and CH4 production. No effect (P > 0.10) of enantiomers, individual acid or interaction acid × concentration was detected at 12 and 24 h for cumulative gas or CH4 production. Addition of DL-malic, L-malic and fumaric acids increased (P < 0.0001) the percentage of propionic acid in the ruminal fluid total VFA compared with all concentrations of the other organic acids or their enantiomers. Ammonia concentration was not affected (P ≥ 0.28) by the addition of organic acids, concentrations or interactions. These findings are evidence that ruminal microorganisms can metabolise both D- and L-enantiomers of organic acids. None of the organic acids and their enantiomers at four different concentrations demonstrated potential as CH4 mitigation agents.

scholarly journals Methionine Hydroxy Analog Supplementation to Increase Feed Utilization for Indigenous Sheep

2020 ◽  
Vol 38 (1) ◽  
pp. 55
Author(s):  
Bambang Waluyo Hadi Eko Prasetiyono ◽  
Mulyono Mulyono ◽  
Widiyanto Widiyanto
Keyword(s):  
Total Protein ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Ruminal Fermentation ◽  
Indigenous Sheep ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Low Efficiency

In the tropical area, productivity of ruminant has not optimized caused by the low quality of nutrition that leads to low-efficiency metabolism at the level of ruminal fermentation, post rumen digestibility, and intermediary metabolism. The study aimed to analyze effect of methionine hydroxy analog (MHA) on ruminal fermentation profiles of indigenous sheep specifically in the increase of ruminant productivity. In vitro utility test was conducted using rumen fluid of the indigenous sheep and sample of rational ration having a proportion of grass and concentrate 30%:70%, dry matter basis. The treatments implemented were three levels of MHA supplementation; T0: 0 g/day, T1: 3 g/day, and T2: 6 g/day. Variables measured were dry matter digestibility (DMD), organic matter digestibility (OMD), production of VFA, NH3, as well as total protein, and molar proportion of partial VFA of rumen fluid. Data were analyzed using analysis of variance (ANOVA) in a completely randomized design (CRD). The 0.2% MHA supplementation increased OMD with the highest production of total protein was from 28.57 mg/g (T0) to 40.49 mg/g (T2) (P<0.05). Meanwhile, the lowest ratio of acetate : propionate was from 2.74 (T0) to 2.33 (T2) (P<0.05). Supplementation of MHA up to 6 g/day concentrate increased the performance of fermentation and/or feed utility. 

scholarly journals Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

2020 ◽  
Author(s):  
Rittikeard Prachumchai ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Cassava Root ◽  
Randomized Design ◽  
Completely Randomized Design

The current work aimed to screen the ruminal cyanide-utilizing bacteria and evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, kinetics of gas, in vitro degradability, and ruminal fermentation. The experiment was conducted in a Completely randomized design (CRD) for a screening of cyanide-utilizing bacteria and the dietary treatments were the level of cyanide at 0, 150, 300, and 450 ppm. A 5 &times; 3 factorial arrangement in a Completely randomized design was used for in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of 0.5 g dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. Adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p &lt; 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm, resulted in an increase in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. Increasing the FCR level to more than 260 g/kg of 0.5 g substrate could increase cumulative gas production (p &lt; 0.05), whereas increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g/kg PELFUR (p &lt; 0.05). Cyanide concentration in rumen fluid decreased with PELFUR (p &lt; 0.05) supplementation. Degradability of in vitro dry matter and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g/kg PELFUR (p &lt; 0.05) in 440 g/kg FCR. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid, as well as the ratio of C2 to C3 among supplementations with FCR (p &lt; 0.05) were significantly different. As the proportion of FCR increased to 530 g/kg of the substrate, the volume of C3 increased by 14.6%. This is the first finding of bacteria in the rumen capable of utilizing cyanide, and cyanide might function as a nitrogen source for bacterial cell synthesis. Inclusion of FCR of 530 g/kg with 30 g/kg PELFUR could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.

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