Duodenal flow of nitrogen following protozoal inoculation of fauna-free sheep fed a diet supplemented with casein or soybean meal

1991 ◽  
Vol 71 (3) ◽  
pp. 793-801 ◽  
Author(s):  
M. Ivan ◽  
M. Hidiroglou ◽  
H. V. Petit
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Rumen Fluid ◽  
Mixed Population ◽  
Corn Silage ◽  
Protein Supplement ◽  
Insoluble Protein ◽  
Acid Detergent Fibre ◽  
Supplemental Protein ◽  
Ciliate Protozoa

Fauna-free sheep, equipped with a rumen cannula and a duodenal re-entrant cannula, were fed a corn silage diet supplemented with casein (CA diet) or soybean meal (SBM diet). Eighteen days later the sheep were inoculated via the rumen cannula with a mixed population of ciliate protozoa. Rumen fluid and duodenal digesta were sampled for 4 d before the inoculation and for 13 d following the inoculation. A stable protozoal population was established within 8 d. Protozoa increased (P < 0.05) the digestion of organic matter in the stomach of sheep fed both the CA and the SBM diet, but the effects of supplemental protein and protein × protozoa interaction were not significant (P > 0.05). The stomach digestion of acid detergent fibre was not affected (P > 0.05) by protozoa or by the source of supplementary protein, but the protein × protozoa interaction was significant (P < 0.05). The disappearance of nitrogen from the stomach was lower (P < 0.01) for the SBM diet than for the CA diet and was increased (P < 0.05) for both diets in the presence of protozoa, but the effect of protein × protozoa interaction was not significant (P > 0.05). The ruminal presence of protozoa decreased the flow from the stomach of individual amino acids (from P < 0.05 to P < 0.001) and of nonammonia nitrogen (P < 0.05). However, the decreases were greater (from P < 0.05 to P < 0.001) for the CA diet than for the SBM diet, but the effects of protein × protozoa interaction were not significant (P > 0.05). The ruminal presence of protozoa increased (P < 0.05) the flow from the stomach of ammonia-N for SBM diet but not for CA diet. The effects of protein and of protein × protozoa interaction were not significant (P > 0.05). It was concluded that although ruminal protozoa do not metabolize casein, their effect of decreasing amino acid flow from the stomach can be greater for diets containing casein (soluble protein) as protein supplement than for those containing soybean meal (insoluble protein). Key words: Duodenal flow, nitrogen, protozoa, casein, soybean meal, sheep

EFFECTS OF VOLATILE FATTY ACID TREATMENT ON THE PROTECTION OF PROTEIN IN THE RUMEN

1974 ◽  
Vol 54 (3) ◽  
pp. 393-401 ◽  
Author(s):  
A. S. ATWAL ◽  
L. P. MILLIGAN ◽  
B. A. YOUNG
Keyword(s):  
Fatty Acid ◽  
Microbial Degradation ◽  
Soybean Meal ◽  
Volatile Fatty Acid ◽  
Rumen Fluid ◽  
Ammonia Concentration ◽  
Protein Supplement ◽  
Size Of Particles ◽  
Supplemental Protein ◽  
Lower Digestive Tract

The increase of ammonia in rumen fluid in response to a single dose of protein supplement was used to assess microbial degradation of proteins in the rumen of sheep and cattle held without feed for about 16 h. The administration of 300 g of soybean meal and promine (50:50) pellets (9.4-mm diam) via the rumen fistula of sheep resulted in a rectilinear increase in ammonia concentration in rumen fluid over a 5-h period. The rates of increase of rumen ammonia from isonitrogenous amounts of untreated and 20% volatile fatty acid (VFA)-treated protein pellets were 9.4 and 1.5 mM/h, respectively. The decrease in the rate of protein degradation was proportional to the amount of VFA incorporated and the size of particles in the supplement. When a protein supplement containing common feed ingredients (soybean meal, herring meal and ground barley) was treated with 15% VFA and fed in meal form or as pellets to sheep and cows, the rumen ammonia was maintained for about 10 h at a lower concentration than that after feeding untreated preparations. Treatment of proteins with VFA may be useful in protecting proteins from microbial degradation in the rumen and allowing provision of supplemental protein to the lower digestive tract.

Herring silage as a protein supplement for young cattle

1991 ◽  
Vol 71 (4) ◽  
pp. 1187-1196 ◽  
Author(s):  
J. W. G. Nicholson ◽  
D. A. Johnson
Keyword(s):  
Amino Acids ◽  
Free Amino ◽  
Crude Protein ◽  
Fish Meal ◽  
Unsaturated Fatty Acids ◽  
Rumen Fluid ◽  
Potassium Sorbate ◽  
Protein Supplement ◽  
Young Cattle ◽  
True Protein

Fish silage made by grinding herring and adding formic acid, β-hydroxytoluene and potassium sorbate was evaluated as a protein supplement for young cattle. Only about 15% of the crude protein in the herring silage was true protein. Ammonia N accounted for 8% of the crude protein and most of the rest was peptides and free amino acids. The crude protein of herring silage was as resistant as fish meal to deamination when fermented in rumen fluid, and more resistant than soybean or casein. The herring silage was readily accepted by Holstein heifers fed hay or grass–legume silage with potatoes (7 kg d−1) and a supplement (1.5 kg d−1). Feed intake and weight gain were similar when the heifers were fed hay with either soybean meal or herring silage but were higher when forage silage replaced the hay. Rumen fluid NH3-N and blood urea levels were normal, even for cattle fed the high non-protein N diet of forage silage with herring silage. The herring silage depressed rumen fluid acetate levels and increased propionate in the heifers fed hay + potatoes, probably because of the unsaturated fatty acids in the herring. Well-made herring silage was a suitable protein supplement for young cattle fed forage and potato diets. Key words: Herring silage, fish silage, potatoes, cattle, protein degradation

Impact of a tannin extract on digestibility, ruminal fermentation and duodenal flow of amino acids in steers fed maize silage and concentrate containing soybean meal or canola meal as protein source

2015 ◽  
Vol 153 (5) ◽  
pp. 943-953 ◽  
Author(s):  
S. C. ÁVILA ◽  
G. V. KOZLOSKI ◽  
T. ORLANDI ◽  
M. P. MEZZOMO ◽  
S. STEFANELLO
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Rumen Fluid ◽  
Protein Source ◽  
Canola Meal ◽  
Ruminal Fermentation ◽  
Tannin Extract ◽  
Maize Silage ◽  
Microbial N

SUMMARYFour Holstein steers (297 ± 56 kg of body weight (BW)) fitted with duodenal cannula and rumen catheter, were housed in metabolism cages and used in a 4 × 4 Latin Square trial to evaluate the effect of both protein source andAcacia mearnsiitannin extract on digestibility, ruminal fermentation, rumen microbial protein synthesis, N utilization and on duodenal flow of individual amino acids. The diet was offered at restricted amount of 25 g of dry matter (DM)/kg BW and consisted of maize silage plus concentrate, in a proportion of 0·7:0·3 (DM basis) respectively. Concentrate was formulated with either soybean meal or canola meal as protein source, with or without 50 g/kg ofA. mearnsiitannin extract (i.e. 15 g/kg of total dietary DM). There was no effect of protein source on most variables. The apparent and true organic matter (OM) digestibilities, as well as neutral detergent fibre (NDF) digestibility were negatively affected by tannin extract inclusion without, however, affecting digestible OM intake. The amount of nitrogen (N) excreted in faeces increased whereas the urinary N excretion decreased in tannin extract treatments. No interaction time × treatment was detected for any rumen variable and no treatment effect was observed for rumen fluid pH and reducing sugars concentration. Rumen fluid concentration of ammonia N was lower for the canola meal plus tannin extract treatment. Rumen concentration ofα-amino compounds was not affected by tannin extract but was higher when canola meal was the protein source. The duodenal flow of OM, total N,α-amino N and non-ammonia non-microbial N increased with tannin extract inclusion, whereas the duodenal flow of microbial N was not affected by treatment. For both protein sources, the amount of most individual amino acids flowing to the duodenum increased due to tannin extract addition. In conclusion, the dietary inclusion of 15 g/kg DM of tannin extract fromA. mearnsiiimproved the amino acid supply independently of whether the protein source was canola meal or soybean meal, without affecting the amino acid profile, to steers fed maize silage plus concentrate, with a minor but significant impact on OM digestibility.

RESPONSE OF LACTATING DAIRY COWS TO FORMALDEHYDE-TREATED SOYBEAN MEAL WHEN FED WITH CONTROL OR UREA-TREATED CORN SILAGE

1986 ◽  
Vol 66 (1) ◽  
pp. 85-95 ◽  
Author(s):  
J. D. ERFLE ◽  
F. D. SAUER ◽  
S. MAHADEVAN ◽  
R. M. TEATHER
Keyword(s):  
Body Weight ◽  
Dairy Cows ◽  
Milk Production ◽  
Soybean Meal ◽  
Dry Matter ◽  
Milk Protein ◽  
Rumen Fluid ◽  
Amino Acid Content ◽  
Negative Control ◽  
Corn Silage

In two experiments, lactating cows (26 in exp. 1; 32 in exp. 2) were fed an 11.3% crude protein (CP) diet, a 14.7% CP diet containing untreated soybean meal (SBM) or a 14.6% CP diet containing formaldehyde-treated SBM (FSBM), all three fed as a complete blended diet with control corn silage (CCS) (exp. 1); a 12% CP diet, a 13.4% CP diet containing SBM or a 13.3% CP diet containing FSBM, all fed as a complete blended diet with urea-treated corn silage (UCS) (exp. 2). In both experiments concentrates and CCS or UCS were mixed (50:50 dry matter) daily and fed ad lib for a 16-wk period. The increase of CP over the low CP negative control by addition of untreated SBM resulted in a significant (P < 0.05) increase in milk production in both experiments. The addition of FSBM had no significant effect. When comparing solids-corrected milk (SCM), addition of SBM, whether treated or not, significantly increased SCM in both experiments. In neither experiment did FSBM improve milk production over untreated SBM. All animals fed CCS lost body weight but there was no difference between treatments. For animals fed UCS the low CP group lost significantly (P < 0.05) more body weight than did the group fed FSBM. Dry matter (DM) intake was significantly (P < 0.05) lower for the FSBM fed group than for the group fed untreated SBM in exp. 1 (CCS). DM intake was significantly (P < 0.05) increased by either FSBM or SBM over the low CP control fed UCS. Milk protein was the only component significantly (P < 0.05) decreased by FSBM in exp. 1. Isobutyric and isovaleric acids were significantly (P < 0.05) lower in rumen fluid of animals on FSBM plus CCS. Rumen microbial protein content seemed to be more adversely affected by FSBM with CCS than UCS. Amino acid content of FSBM and SBM demonstrated that lysine and tyrosine were lost from SBM after treatment with formaldehyde. The lack of a response in milk production to FSBM is discussed in terms of a lysine and tyrosine deficiency for milk protein synthesis and the adverse effect that formaldehyde protection of SBM may have on the rumen microbial population. Key words: Lactation, dairy cows, urea, corn silage, soybean meal, formaldehyde treatment

scholarly journals INFLUENCE OF SUPPLEMENTAL DIETARY COPPER ON COPPER CONCENTRATION OF PIG BLOOD, SELECTED TISSUES AND DIGESTIVE TRACT CONTENTS

1970 ◽  
Vol 50 (1) ◽  
pp. 113-120 ◽  
Author(s):  
N. J. DROULISCOS ◽  
J. P. BOWLAND ◽  
J. I. ELLIOT
Keyword(s):  
Blood Plasma ◽  
Soybean Meal ◽  
Unsaturated Fatty Acids ◽  
Dietary Treatment ◽  
Major Influence ◽  
Protein Supplement ◽  
Intestinal Tissue ◽  
Dietary Copper ◽  
Intestinal Contents ◽  
Supplemental Protein

Twenty-four pigs received barley-based diets with either fishmeal or soybean meal as supplemental protein and either 0 or 250 ppm supplemental Cu. Supplemental Cu increased rate of gain for fishmeal-supplemented pigs but not for soybean meal-supplemented pigs.Cu concentration of intestinal tissue (wet basis) from the duodenum, two sections of jejunum, the ileum and colon averaged 17 ppm and of intestinal contents averaged 28 ppm when either protein supplement without Cu was fed. Addition of Cu to the diets increased concentration of Cu in both intestinal tissue (average 104 ppm) and contents (average 253 ppm), but source of protein did not influence the increase. The liver (wet basis) averaged 29 ppm Cu for unsupplemented pigs fed either protein supplement, 166 ppm for Cu-supplemented pigs fed soybean meal and 329 ppm for Cu-supplemented pigs fed fish-meal. Cu levels in portal blood plasma averaged 21 μg/100 ml and were not significantly influenced by dietary treatment, sex or weight. Cu in systemic blood plasma averaged 110 μg/100 ml. Unsaturated fatty acids were increased in the backfat of pigs receiving the fishmeal-supplemented diet with Cu.Weight at slaughter (50, 70 or 90 kg) influenced Cu concentration only in contents of the colon and in the liver. Gilts did not differ from barrows in Cu concentration in tissues or intestinal contents. The results suggest that the intestinal mucosa has a major influence on the control of systemic Cu levels.

VALEUR ALIMENTAIRE DE L'ENSILAGE DE MAIS-LUZERNE POUR L'ALIMENTATION DES BOUVILLONS

1982 ◽  
Vol 62 (3) ◽  
pp. 959-961
Author(s):  
PAUL FLIPOT ◽  
S. PAGE ◽  
G. LALANDE
Keyword(s):  
Key Words ◽  
Soybean Meal ◽  
Corn Silage ◽  
Protein Supplement ◽  
Alfalfa Silage

Sixty-six Hereford steers were fed rations of corn silage, or corn silage plus 400 g∙head−1∙day−1 of soybean meal, or a mixture of corn and alfalfa silage (ratio 1:1 on a wet basis at ensiling). Alfalfa, when mixed with forage corn at ensiling, can be used as a protein supplement to replace a part of the soybean meal but corn silage alone may not be recommended. Key words: Ensilage, maïs-luzerne, bouvillons

Protection of proteins from degradation in the rumen by heating in the presence of sugars

1989 ◽  
Vol 1989 ◽  
pp. 45-45
Author(s):  
R. J. Wallace
Keyword(s):  
Amino Acids ◽  
Effective Treatment ◽  
Soybean Meal ◽  
Treatment Process ◽  
Homogeneous Model ◽  
Enzymatic Browning ◽  
Protein Supplement ◽  
Host Animal ◽  
Alternative Method

Heat and aldehyde treatments have been widely used to protect proteins from degradation in the rumen, and conditions that lead to an undegradable, but still digestible, product (bypass protein; BP) have been determined. The problem with these treatments is that the conditions that are used are quite severe, and damage to amino acids, particularly lysine, occurs. Excessive treatment can also yield an indigestible product. Recently, an alternative method involving the heating of soybean meal under milder conditions (150°C, 1 h) in the presence of glucose, fructose, xylose or lactose (non-enzymatic browning) was shown to yield a product high in UDP, but in which the lysine was predicted to remain available to the host animal [1-3].In the present experiments, casein was used as a homogeneous model substrate to investigate how wide a range of conditions can be used for effective treatment of proteins with sugars. Although casein is atypical in that it is one of the most rapidly degraded proteins available, the results point to a versatility in the sugar treatment process that will enable different sugars to be used according to the type of process available. Preliminary experiments with soybean meal suggested that an effective way of optimising this effect may be to encapsulate the protein supplement with a sugar-protein mix.

RAPESEED MEAL VERSUS SOYBEAN MEAL AS THE ONLY PROTEIN SUPPLEMENT FOR LACTATING COWS FED CORN SILAGE ROUGHAGE RATIONS

1973 ◽  
Vol 53 (1) ◽  
pp. 107-112 ◽  
Author(s):  
D. E. WALDERN
Keyword(s):  
Milk Production ◽  
Soybean Meal ◽  
Milk Fat ◽  
Nitrogen Retention ◽  
Rapeseed Meal ◽  
Corn Silage ◽  
Lactating Cows ◽  
Daily Milk Production ◽  
Protein Milk ◽  
Supplemental Protein

Digestion, nitrogen balance, and a 112-day lactation trial were conducted with Holstein–Friesian cows fed corn silage as the only roughage, and concentrate rations containing either rapeseed meal (RSM) or soybean meal (SBM) as the only source of supplemental protein. The RSM and SBM consumed in the lactation trial represented 11.8 and 10.6% of the total daily dry matter intake, respectively. Digestibility of total ration crude protein was lower (P < 0.05) for RSM- than SBM-supplemented cows; nitrogen retention and efficiency of nitrogen utilization, however, was comparable for cows in the two treatment groups. Daily milk production, 4% fat-corrected milk production, percent milk fat, protein, and nonfat solids were greater (P < 0.05) for cows fed concentrate containing SBM than for those fed RSM as the source of supplemental protein. Milk production declined more for cows fed RSM compared with those fed SBM during the 112-day trial.

scholarly journals The alleviation of chronic copper toxicity in sheep by ciliate protozoa

1986 ◽  
Vol 55 (2) ◽  
pp. 361-367 ◽  
Author(s):  
M. Ivan ◽  
D. M. Veira ◽  
C. A. Kelleher
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Copper Toxicity ◽  
Live Weight ◽  
Soluble Proteins ◽  
Mixed Population ◽  
The Other ◽  
Feed Consumption ◽  
Cu Toxicity ◽  
Ciliate Protozoa

1. Rams, fauna-free from birth and initially of 48–65 kg live weight, were allocated to two groups of ten each and given a diet containing 14 μg copper/g dry matter; five additional rams were killed and their livers were analysed for Cu.2. One group (faunated) was inoculated with a mixed population of ciliate protozoa, and contained between 60x105and 195 x 105protozoa/ml rumen fluid throughout the 184 d experiment. The other group remained fauna-free. Following blood sampling, three rams in each group were killed on day 63, two on day 125 and four on day 184. One sheep in each group died during the experiment.3. Faunated rams showed higher weight gains and feed consumption than fauna-free rams.4. Plasma Cu concentration (μg/ml) increased from an initial 0.82to a final 1.00 in faunated and 1.36 in fauna-free rams. Liver Cu concentration (μg/g dry matter) increased from an initial 745 to a final 962 and 1684 in faunated and in fauna-free sheep respectively, representing a 4.3-fold greater increase in the fauna-free than in the faunated group. The absorption and retention of Cu was 38–50% higher in the fauna-free than in the faunated rams.5. It was suggested that rumen ciliate protozoa increased rumen production of sulphide (through increased breakdown of soluble proteins) which complexed part of the Cu, making it unavailable for absorption and utilization. Therefore, ciliate protozoa could determine susceptibility to chronic Cu toxicity in sheep.

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