Effects of a parasympathomimetic agent, slaframine, on reticulo-omasal orifice function

1991 ◽  
Vol 71 (2) ◽  
pp. 321-326 ◽  
Author(s):  
J. M. Kelly ◽  
B. W. McBride ◽  
M. A. Froetschel ◽  
W. J. Croom Jr. ◽  
W. M. Hagler Jr.
Keyword(s):  
Key Words ◽  
Pressure Transducer ◽  
Latin Square ◽  
Holstein Cows ◽  
Characteristic Change ◽  
Once Daily ◽  
Contraction Frequency ◽  
Ad Libitum ◽  
Digesta Passage ◽  
Orifice Opening

Three nonlactating, nonpregnant, ruminally cannulated Holstein cows (680–732 kg) fed chopped brome hay ad libitum once daily were used to study effects of slaframine (SF) on rumen function in a 3 × 3 Latin square design. Animals were injected intramuscularly with 0, 20 or 40 μg kg BW−1 SF, allowing for 1 d between trials. At 0, 40, 180 and 360 min postinjection rumen contents were partially evacuated. Reticular contraction frequency (RCF) was determined using a balloon placed in the reticulum connected to a pressure transducer and physiograph and reticulo-omasal orifice opening duration (ROOD) was measured using palpation and endoscopy. RCF as a percentage of predose was decreased linearly by SF treatment (P < 0.01). SF affected ROOD, which was higher (P < 0.05) for both SF treatments. This pattern was also linear (P < 0.01). A characteristic change in reticulum pressure was identified that correlated with SF mediated variation in ROOD (r = 0.67; P < 0.01). Previous research has indicated that SF administration increased salivation and shifted site of digestion. The results presented herein are consistent with these observations and indicate that duration of reticulo-omasal orifice opening may be more highly associated with the rate of digesta passage than reticular frequency. Key words: Slaframine, reticulo-omasal orifice, reticulum motility, cattle

Effects of abomasal slaframine infusion on ruminal digesta passage and digestion in steers

1995 ◽  
Vol 75 (1) ◽  
pp. 157-163 ◽  
Author(s):  
M. A. Froetschel ◽  
M. N. Streeter ◽  
H. E. Amos ◽  
W. J. Croom Jr. ◽  
W. M. Hagler Jr.
Keyword(s):  
Dilution Rate ◽  
Latin Square ◽  
Saline Control ◽  
Net Energy ◽  
Once Daily ◽  
Abomasal Infusion ◽  
Muscarinic Cholinergic ◽  
Digesta Passage ◽  
Sorghum Silage ◽  
Ruminal Digestion

Slaframine was infused intra-abomasally once daily 3 h after feeding to four ruminally and abomasally cannulated Jersey steers (586 ± 88.6 kg) at 0 (saline control), 10, 20, or 30 μg kg−1 BW in a 4 × 4 Latin square design experiment. Periods were 14 d in length. On a DM basis, diets were a 38:62, sorghum silage: concentrate offered at 125% of the net energy required for maintenance. Ruminal, abomasal, and fecal samples were collected at 2-h intervals on days 10–12, and ruminal contents were completely evacuated and sampled at 3, 6, 12, and 24 h after feeding on days 13 and 14. Ruminal volume and pH increased (linear; P < 0.05) from 1.6 to 8.9% and from 0.10 to 0.22 units, respectively, with SF. Total ruminal VFA and ammonia concentrations decreased (linear; P < 0.05) with SF from 4.1 to 9.4% and from 5.3 to 10.2%, respectively. Apparent ruminal DM and starch digestion increased at the lowest dosage and decreased at higher dosages of SF (quadratic; P < 0.05). Ruminal NDF digestion decreased with SF (linear; P < 0.1). Totat tract DM digestion was not affected by SF. Fractional turnover rate of ruminal DM increased (linear; P < 0.05) from 1.6 to 11.3% with SF. During the first 8 h after SF administration, liquid dilution rate increased as much as 18.5% with the highest dosage of SF (linear; P < 0.05). During the 10– to 21-h period after SF administration, dilution rate decreased with the 10– to 20-μg kg−1 BW dosages of SF (quadratic, P < 0.05). Outflow of AA tended to decrease at 10 and 20 and increased at the 30 μg kg−1 BW dosages of SF (quadratic, P < 0.05). Abomasal infusion of SF alters digesta passage and the site of digestion of nutrients between the rumen and the lower digestive tract. The highest level shifted the site of digestion to the lower tract while lower dosages enhanced ruminal digestion. Key words: Rumen fermentation, digestion, passage, muscarinic, cholinergic

EFFECTS OF PSYCHOLOGICAL STRESS, ACUTE COLD STRESS, AND DIET ON FORESTOMACH CONTRACTIONS IN CATTLE

1988 ◽  
Vol 68 (2) ◽  
pp. 399-407 ◽  
Author(s):  
A. LIRETTE ◽  
J. M. KELLY ◽  
L. P. MILLIGAN ◽  
R. J. CHRISTOPHERSON
Keyword(s):  
Heart Rate ◽  
Cold Stress ◽  
Key Words ◽  
Psychological Stress ◽  
Latin Square ◽  
Alfalfa Hay ◽  
Alfalfa Silage ◽  
Acute Psychological Stress ◽  
Key Words Cold

Studies were made on forestomach contraction frequencies as affected by diet (brome hay, alfalfa hay, alfalfa silage and barley concentrate diet), acute cold stress and acute psychological stress. Four 15-mo-old steers fitted with ruminal fistulae were used in a 4 × 4 Latin square design. Diets did not influence contractile frequencies of the forestomachs, and did not interact with the effects of the stresses. Acute cold stress and psychological stress both produced significant increases of forestomach contraction frequencies and of heart rate. Key words: Cold stress, psychological stress, forestomach contractions, heart rate

Effects of feeding or abomasal infusion of canola oil in Holstein cows. 2. Gene expression and plasma concentrations of cholecystokinin and leptin

2004 ◽  
Vol 71 (3) ◽  
pp. 288-296 ◽  
Author(s):  
Prasanth K Chelikani ◽  
David R Glimm ◽  
Duane H Keisler ◽  
John J Kennelly
Keyword(s):  
Gene Expression ◽  
Feed Intake ◽  
Canola Oil ◽  
Unsaturated Fatty Acids ◽  
Plasma Concentrations ◽  
Latin Square ◽  
Basal Diet ◽  
Holstein Cows ◽  
Abomasal Infusion ◽  
Supplemental Fat

We determined the relative importance of cholecystokinin (CCK), leptin, and fatty acid concentrations in plasma in mediating the satiety effects of supplemental fat in lactating cows. Five ruminally and duodenally cannulated Holstein cows in late lactation were used in a 3×5 incomplete Latin square design with three treatments: 1) Control: basal diet (CON), 2) Control+supplementation of canola oil at 1 kg/d in the feed (FED) and 3) Control+abomasal infusion of canola oil at 1 kg/d (INF). Relative to CON, feed intake was reduced by INF but not by FED. We provide evidence that both FED and INF treatments stimulated CCK gene expression in the duodenum and elevated plasma CCK concentrations. However, our results did not support a role for CCK in mediating satiety through an endocrine mechanism of action. We speculate that CCK might be acting either through paracrine and/or neurocrine routes to influence feed intake in cattle. Both FED and INF had no effect on the mRNA abundance of leptin, lipoprotein lipase, or acetyl-CoA carboxylase in adipose tissue. Plasma concentrations of leptin, insulin and IGF-I were not altered by FED or INF, indicating that these signals may not be involved in mediating short-term hypophagic effects of dietary fat. Plasma concentrations of 18[ratio ]1n-9 and 18[ratio ]2n-6 were significantly greater for INF than for FED or CON. We conclude that the hypophagic effects of supplemental fat in cattle depend on the amount of unsaturated fatty acids reaching the intestine and that this satiety effect is mediated through CCK, oleic acid and (or) linoleic acid, but leptin is not involved.

scholarly journals Replacing grass silage with pea-barley intercrop silage in the feeding of the dairy cow

2008 ◽  
Vol 15 (3) ◽  
pp. 235 ◽  
Author(s):  
P. PURSIAINEN ◽  
M. TUORI
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Protein Concentration ◽  
Milk Protein ◽  
Latin Square ◽  
Crude Protein Content ◽  
Grass Silage ◽  
Ad Libitum ◽  
Ad Libitum Intake ◽  
Barley Silage

The effect of replacing wilted grass silage (GS) with pea-barley intercrop silage (PBS) on feed intake, diet digestibility and milk production was studied with 8 multiparous Ayrshire-cows in a replicated 4 × 4 Latin square experiment. Proportion of PBS was 0 (PBS0), 33 (PBS33), 67 (PBS67) or 100 (PBS100) % of silage dry matter (DM). The DM content was 559 and 255 g kg-1 for GS and PBS. Crude protein content was 131 and 170 g kg-1 DM, respectively. Pea-barley silage was more extensively fermented than GS with total fermentation acid content of 120 vs. 12 g kg-1 DM. Silage was fed for ad libitum intake and supplemented with on the average 13 kg concentrate per day. Silage DM intake was 9.2 (PBS0), 9.7 (PBS33), 9.0 (PBS67) and 7.1 (PBS100) kg per day (Pquadr. < 0.05). The energy corrected milk yield [30.3 (PBS0), 29.8 (PBS33), 30.3 (PBS67), 31.3 (PBS100) kg per day] was not significantly affected by the treatment. Milk protein concentration decreased linearly (P < 0.05) in response to feeding PBS. It is concluded that PBS can replace up to two thirds of wilted, moderate quality GS in the feeding of dairy cows because in this experiment pure pea-barley silage reduced silage intake.

Effect of offering three amounts of maize stover to dairy cows on intake and selection

1997 ◽  
Vol 1997 ◽  
pp. 85-85
Author(s):  
J. N. Methu ◽  
E. Owen ◽  
A. Abate ◽  
M. Scarr ◽  
J. Tanner
Keyword(s):  
Dairy Cows ◽  
Crude Protein ◽  
Feeding Strategy ◽  
Latin Square ◽  
Live Weight ◽  
Leaf Sheath ◽  
Barley Straw ◽  
Maize Stover ◽  
Ad Libitum ◽  
Friesian Cows

Several studies with barley straw (e.g. Wahed et al, 1990) and sorghum stover (e.g. Osafo, 1993) have shown improvements in intake with increasing ‘ad libitum’ amounts offered. Furthermore, these studies have demonstrated that sheep and goats offered barley straw or sorghum stover in long, unprocessed form, increase intake by selecting for leaf and leaf sheath, and against stem. This phenomenon offers a simple feeding strategy to use selective feeding behaviour to improve intake and hence production. The aim of the present experiment was to study the effect of offering increasing amounts of long maize stover on intake and selection by dairy cows.Six, early- to mid-lactation Ayrshire and Friesian cows (live weight, M, 430 kg) were used in a double 3x3 Latin square design with 24-day (d) feeding periods. Cows were offered long (unchopped) maize stover at low, medium or high ‘ad libitum’ rates, i.e. 31, 59 or 87 g dry matter (DM)/kg M.d. Cows also received 3.2 kg DM/d of cotton seed cake (339 g/kg DM crude protein) in two meals at milking (0700 h and 1800 h). Stover was offered in one meal daily, at 0700 h, after collecting refusals from the previous day. Amounts of stover offered and refused were weighed daily. Samples of offered stover (0.5 kg) and refused stover (0.5 kg) were analysed for DM daily. All refused stover and 4.0 kg samples of offered stover were botanically fractionated, daily, into stem (S), leaf (L), sheath (Sh) and husk (H). Milk yield was recorded daily and cows weighed at the start and end of each period.

ACETATE AND FREE FATTY ACID METABOLISM IN THE FAT-TAILLED SHEEP

1984 ◽  
Vol 64 (5) ◽  
pp. 244-245 ◽  
Author(s):  
J. G. VAN DER WALT ◽  
A. MARAIS ◽  
S. CAMPBELL ◽  
J. M. GOODEN
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Key Words ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Carbon Flow ◽  
Whole Body ◽  
Free Fatty Acid Metabolism ◽  
Ad Libitum ◽  
Ad Libitum Feeding

Two-thirds of all acetate to FFA conversion takes place in the hindquarter of Karakul lambs fed ad libitum. An amount equivalent to two-thirds of this net FFA production is taken up by the hind-quarter. Half ad libitum feeding resulted in halving the whole-body acteate turnover, reversing the direction of carbon flow from FFA to acetate and doubling the FFA turnover, chiefly by mobilizing tail fat. Resumption of ad libitum feeding reversed the process, without synthesizing FFA from acetate. Key words: Sheep, growth, fat-tail, FFA, acetate, turnover

scholarly journals Altering the temporal distribution of energy intake with isoenergetically dense foods given as snacks does not affect total daily energy intake in normal-weight men

2000 ◽  
Vol 83 (1) ◽  
pp. 7-14 ◽  
Author(s):  
A. M. Johnstone ◽  
E. Shannon ◽  
S. Whybrow ◽  
C. A. Reid ◽  
R. J. Stubbs
Keyword(s):  
Food Intake ◽  
Energy Density ◽  
Energy Intake ◽  
Real Life ◽  
Latin Square ◽  
Temporal Distribution ◽  
Normal Weight ◽  
Daily Energy ◽  
Fixed Composition ◽  
Ad Libitum

The objectives of the present study were to examine the effects of (1) ingesting mandatory snacks v. no snacks and (2) the composition of isoenergetically-dense snacks high in protein, fat or carbohydrate, on food intake and energy intake (EI) in eight men with ad libitum access to a diet of fixed composition. Subjects were each studied four times in a 9 d protocol per treatment. On days 1–2, subjects were given a medium-fat maintenance diet estimated at 1·6 × resting metabolic rate (RMR). On days 3–9, subjects consumed three mandatory isoenergetic, isoenergetically dense (380 kJ/100 g) snacks at fixed time intervals (11.30, 15.30 and 19.30 hours). Total snack intake comprised 30 % of the subjects' estimated daily energy requirements. The treatments were high protein (HP), high carbohydrate (HC), high fat (HF) and no snack (NS). The order was randomized across subjects in a counterbalanced, Latin-square design. During the remainder of the day, subjects had ad libitum (meal size and frequency) access to a covertly manipulated medium-fat diet of fixed composition (fat: carbohydrate: protein, 40:47:13 by energy), energy density 550 kJ/100 g. All foods eaten were investigator-weighed before ingestion and left-overs were weighed after ingestion. Subjective hunger and satiety feelings were tracked hourly during waking hours using visual analogue scales. Ad libitum EI amounted to 13·9 MJ/d on the NS treatment compared with 11·7, 11·7 and 12·2 MJ/d on the HP, HC and HF diets respectively (F(3,21) 5·35; P = 0·007, sed 0·66). Total EI values were not significantly different at 14·6, 14·5, 15·0 and 14·2 MJ/d respectively. Snack composition did not differentially affect total daily food intake or EI. Average daily hunger was unaffected by the composition of the snacks. Only at 12.00 hours did subjects feel significantly more hungry during the NS condition, relative to the other dietary treatments (F(3,18) 4·42; P = 0·017). Body weight was unaffected by dietary treatment. In conclusion, snacking per se led to compensatory adjustments in feeding behaviour in lean men. Snack composition (with energy density controlled) did not affect the amount eaten of a diet of fixed composition. Results may differ in real life where subjects can alter both composition and amount of food they eat and energy density is not controlled.

Effect of Metabolisable Protein on intake of grass silage and milk protein yield in dairy cows.

1994 ◽  
Vol 1994 ◽  
pp. 113-113 ◽  
Author(s):  
J.R. Newbold ◽  
B.R. Cottrill ◽  
R.M. Mansbridge ◽  
J.S. Blake
Keyword(s):  
Dairy Cows ◽  
Milk Protein ◽  
Live Weight ◽  
Protein Yield ◽  
Holstein Cows ◽  
Grass Silage ◽  
Lactating Dairy Cows ◽  
Ad Libitum ◽  
Few Data

Few data are available to evaluate the Metabolisable Protein (MP) System (AFRC, 1992) as an aid to ration formulation for dairy cows. Responses of lactating dairy cows to MP were evaluated in two experiments.In Experiment One, groups of 10 Holstein cows were offered grass silage ad libitum and 7kg DM/d of one of six (treatments 1-6) compound foods from 39d after calving, for eight weeks. All rations were isoenergetic, assuming silage intake of 11kg DM/d (typical for the herd). Ratios between MP supply (calculated from in situ protein degradability measurements) and requirement (calculated for a standard cow: 565kg live weight, 281 milk/d, 40g/kg butterfat, 33g/kg milk protein, liveweight change =0kg/d) were 1.00, 1.02, 1.06, 1.06, 1.1 and 1.17 for treatments 1-6, respectively.

scholarly journals The effect of protein degradation products in grass silages on feed intake and intake behaviour in sheep

1995 ◽  
Vol 73 (1) ◽  
pp. 51-64 ◽  
Author(s):  
M. Van Os ◽  
J.P. Dulphy ◽  
R. Baumont
Keyword(s):  
Treatment Effects ◽  
Degradation Products ◽  
Latin Square ◽  
Dietary Treatment ◽  
Intake Rate ◽  
Once Daily ◽  
Rumen Ph ◽  
Chewing Efficiency ◽  
Amine Addition ◽  
Intake Regulation

The effects of NH3 and amines on grass-silage intake, intake behaviour and rumen characteristics were studied in sheep. From a single sward, two direct-cut grass silages were prepared, either untreated (WAS) or with 4·51 formic acid/tonne (FAS). Four experimental diets: WAS, FAS, FAS with addition of 2·9 g NH3/kg DM (FAS + N) and FAS with 2·8 g amines/kg DM (FAS + A), were offered ad lib. once daily to four rumen-cannulated wethers in a 4 × 4 Latin square design. Daily DM intake (DMI) tended to be influenced by dietary treatment (P = 0·09). Compared with FAS, DMI was lower for WAS. Addition of NH3 did not alter DMI, whereas amine addition slightly lowered daily DMI. Reduced DMI resulted from lower intake rates during both the principal meal and the subsequent small meals. Lower initial intake rate during the principal meal suggested reduced palatability of WAS and FAS + A. Amines and NH3, however, did not influence chewing efficiency. No treatment effects were observed on total rumen pool size, DM and neutral-detergent fibre content. Furthermore, NH3, and amines did not alter rumen pH, NH3, and volatile fatty acid concentrations to the extent that they could act on chemostatic intake regulation. Amine addition, however, lowered osmolality of the rumen liquid. No treatment effects on rumen motility were observed. In conclusion, daily DMI was not reduced by the addition of NH3, suggesting that NH3per se is not the causal factor in the negative correlations between silage NH3 content and intake observed by other authors. Amines, however, tended to reduce DMI only by their effect at the oro-pharyngeal level of intake control.

EFFECT OF ADDING POTENTIAL VOMITOXIN (DEOXYNIVALENOL) DETOXICANTS OR A F. graminearum INOCULATED CORN SUPPLEMENT TO WHEAT DIETS FED TO PIGS

1984 ◽  
Vol 64 (3) ◽  
pp. 733-741 ◽  
Author(s):  
D. W. FRIEND ◽  
H. L. TRENHOLM ◽  
K. E. HARTIN ◽  
J. C. YOUNG ◽  
B. K. THOMPSON
Keyword(s):  
Key Words ◽  
Fusarium Graminearum ◽  
Ammonium Carbonate ◽  
Control Diet ◽  
Feed Consumption ◽  
Binding Agent ◽  
Stomach Mucosa ◽  
Ad Libitum

Six groups each of five barrows and five gilts were offered ad libitum one of six pelleted diets containing 68% wheat. A pellet binder was added (1.5%) to all diets. There were three clean wheat diets, one unsupplemented, one supplemented with a binding agent (Antitox Vana) and another with Fusarium graminearum inoculated corn; also, three vomitoxin-contaminated wheat diets, one unsupplemented, one supplemented with Antitox Vana and another with ammonium carbonate. Vomitoxin content was negligible in the clean wheat (control) diet, and was about 5 mg/kg in the three contaminated diets and 14 mg/kg in the inoculated corn diet. Pigs weighed 35 kg initially and a necropsy was conducted on each after 5 wk on experiment. Feed consumption was reduced 15–17% with the contaminated wheat diets, and approximately 50% with the inoculated corn diet from which effect the pigs seemed never to recover to the level of those fed the contaminated wheat diets. Discoloration of the stomach mucosa, and epithelial thickening of the esophageal area occurred in more pigs fed the contaminated wheat diets than those fed the clean wheat (control) diet, and a more marked response was evoked by the inoculated corn diet. The addition of the binding agent or the ammonium carbonate did not appear in this experiment to alleviate any of these effects. Key words: Vomitoxin, deoxynivalenol, antitoxin, wheat, inoculated corn, pigs

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