MUSCLE AND RECTAL TEMPERATURE RESPONSE CURVES TO A SHORT-TERM HALOTHANE CHALLENGE IN EIGHT-WEEK-OLD PIGLETS WITH KNOWN GENOTYPE AT THE HALOTHANE LOCUS

1990 ◽  
Vol 70 (1) ◽  
pp. 9-14 ◽  
Author(s):  
A. P. SATHER ◽  
A. L. SCHAEFER ◽  
A. K. W. TONG ◽  
C. GARIÉPY ◽  
S. M. ZAWADSKI

Each of the three genotypes (NN: normal, halothane negative; Nn: carrier, halothane negative; nn: halothane sensitive) at the halothane locus had a significantly different muscle temperature response curve to a 3-min halothane challenge, while only halothane positive (H+) and negative H−) phenotypes could be distinguished on the basis of the rectal temperature response curves. However, the among animal variation precludes its use as a diagnostic tool for the identification of heterozygous and homozygous normal among halothane negative pigs. Key words: Temperature, halothane gene, swine, genotype

2016 ◽  
Vol 13 (24) ◽  
pp. 6651-6667 ◽  
Author(s):  
Jing Tang ◽  
Guy Schurgers ◽  
Hanna Valolahti ◽  
Patrick Faubert ◽  
Päivi Tiiva ◽  
...  

Abstract. The Arctic is warming at twice the global average speed, and the warming-induced increases in biogenic volatile organic compounds (BVOCs) emissions from Arctic plants are expected to be drastic. The current global models' estimations of minimal BVOC emissions from the Arctic are based on very few observations and have been challenged increasingly by field data. This study applied a dynamic ecosystem model, LPJ-GUESS, as a platform to investigate short-term and long-term BVOC emission responses to Arctic climate warming. Field observations in a subarctic tundra heath with long-term (13-year) warming treatments were extensively used for parameterizing and evaluating BVOC-related processes (photosynthesis, emission responses to temperature and vegetation composition). We propose an adjusted temperature (T) response curve for Arctic plants with much stronger T sensitivity than the commonly used algorithms for large-scale modelling. The simulated emission responses to 2 °C warming between the adjusted and original T response curves were evaluated against the observed warming responses (WRs) at short-term scales. Moreover, the model responses to warming by 4 and 8 °C were also investigated as a sensitivity test. The model showed reasonable agreement to the observed vegetation CO2 fluxes in the main growing season as well as day-to-day variability of isoprene and monoterpene emissions. The observed relatively high WRs were better captured by the adjusted T response curve than by the common one. During 1999–2012, the modelled annual mean isoprene and monoterpene emissions were 20 and 8 mg C m−2 yr−1, with an increase by 55 and 57 % for 2 °C summertime warming, respectively. Warming by 4 and 8 °C for the same period further elevated isoprene emission for all years, but the impacts on monoterpene emissions levelled off during the last few years. At hour-day scale, the WRs seem to be strongly impacted by canopy air T, while at the day–year scale, the WRs are a combined effect of plant functional type (PFT) dynamics and instantaneous BVOC responses to warming. The identified challenges in estimating Arctic BVOC emissions are (1) correct leaf T estimation, (2) PFT parameterization accounting for plant emission features as well as physiological responses to warming, and (3) representation of long-term vegetation changes in the past and the future.


2016 ◽  
Author(s):  
Cristian A. Solari ◽  
Vanina J. Galzenati ◽  
Brian J. McGill

AbstractAlthough there is a well developed theory on the relationship between the intrinsic growth rate r and temperature T, it is not yet clear how r relates to abundance, and how abundance relates to T. Many species often have stable enough population dynamics that one can talk about a stochastic equilibrium population size N*. There is sometimes an assumption that N* and r are positively correlated, but there is lack of evidence for this. To try to understand the relationship between r, N*, and T we used a simple chemostat model. The model shows that N* not only depends on r, but also on the mortality rate, the half-saturation constant of the nutrient limiting r, and the conversion coefficient of the limiting nutrient. Our analysis shows that N* positively correlates to r only with high mortality rate and half-saturation constant values. The response curve of N* vs. T can be flat, Gaussian, convex, and even temperature independent depending on the values of the variables in the model and their relationship to T. Moreover, whenever the populations have not reached equilibrium and might be in the process of doing so, it could be wrongly concluded that N* and r are positively correlated. Because of their low half-saturation constants, unless conditions are oligotrophic, microorganisms would tend to have flat abundance response curves to temperature even with high mortality rates. In contrast, unless conditions are eutrophic, it should be easier to get a Gaussian temperature response curve for multicellular organisms because of their high half-saturation constant. This work sheds light to why it is so difficult for any general principles to emerge on the abundance response to temperature. We conclude that directly relating N* to r is an oversimplification that should be avoided.


2016 ◽  
Author(s):  
Jing Tang ◽  
Guy Schurgers ◽  
Hanna Valolahti ◽  
Patrick Faubert ◽  
Päivi Tiiva ◽  
...  

Abstract. The Arctic is warming at twice the global average speed, and the warming-induced increases in biogenic volatile organic compounds (BVOC) emissions from arctic plants are expected to be drastic. The current global models' estimations of minimal BVOC emissions from the Arctic are based on very few observations and have been challenged by increasing field data. This study applied a dynamic ecosystem model, LPJ-GUESS, as a platform to investigate short-term and long-term BVOC emission responses to climate warming. Field observations in a subarctic heath tundra with long-term (13 years) warming treatments were extensively used for parameterizing and evaluating BVOC related processes. We proposed an adjusted temperature (T) response curve for arctic plants with much stronger T sensitivity than the commonly-used algorithms for large-scale modelling. The simulated emission responses to 2 °C warming between the adjusted and original T response curves in the model were evaluated against the observed warming responses (WR) at short-term scales. Moreover, the model's responses to higher levels' warming (4 °C and 8 °C) were also investigated as a sensitivity test. The model was able to reproduce vegetation CO2 fluxes as well as day-to-day variability of isoprene and monoterpene emissions. The modelled BVOC WR, especially for isoprene, were better captured by using the adjusted T response curve, comparing with using the original one. A few days' underestimation of leaf T led to the underestimated emission rates as well as WR. During 1999–2012, the modelled annual mean isoprene and monoterpene emissions were 20 and 8 mg C m−2 yr−1, with an increase in emission by 55 % and 57 % for 2 °C summertime warming, respectively. Warming by 4 °C and 8 °C further elevated isoprene emission for all years compared with 2 °C warming, but the impacts on monoterpene emissions levelled off because of a decreased coverage of monoterpene-emitters among the evergreen prostrate dwarf shrubs. The high WR captured by the adjusted T response curve highlight the strong T sensitivity of arctic plants. At short-term scale, the WR seem to be strongly impacted by leaf T; while at long-term scale, the WR are a combined effect of plant functional type (PFT) dynamics as well as instantaneous BVOC responses to warming. The identified essential issues associated with estimating arctic BVOC emissions are: (1) leaf T estimation/extrapolation based on air T; (2) PFT parameterization accounting for BVOC emission features as well as PFT's responses to warming; and (3) representation of vegetation dynamics in the past and the future.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10835
Author(s):  
Seo Yeol Choi ◽  
Min Ho Seo ◽  
Ho Young Soh

The short-term variation in the abundance of Acartia copepods in the eutrophic Gamak Bay of South Korea was investigated with weekly measurements from October 2007 to September 2008. During this period, four Acartia species (A. erythraea, A. ohtsukai, A. omorii, and A. sinjiensis) were recorded as showing seasonally different peak abundance. The abundance of A. erythraea and A. sinjiensis was high in autumn, whereas that of A. omorii was high from winter to spring. In summer, A. erythraea, A. ohtsukai, and A. sinjiensis coexisted at peak abundance significantly related to water temperature and salinity. Results from the response curves of the four Acartia species to water temperature and salinity suggest that A. erythraea and A. sinjiensis increased in abundance at water temperatures >18 °C, whereas A. ohtsukai increased in abundance at water temperatures >27 °C. The occurrence of A. erythraea, A. ohtsukai, and A. sinjiensis decreased with increasing salinity, but chlorophyll-a concentration showed no effect on occurrence. Despite these findings, the coexistence of the three ecologically similar species may be due to prey abundance in summer and autumn (chlorophyll-a concentration >10 µg L−1). Notably, the wide range of the response curve of A. omorii indicates its occurrence at higher salinity levels than other species.


1991 ◽  
Vol 65 (02) ◽  
pp. 160-164 ◽  
Author(s):  
Marina Poggio ◽  
Armando Tripodi ◽  
Guglielmo Mariani ◽  
Pier Mannuccio Mannucci ◽  

SummaryBeing a putative predictor of ischemic heart disease, the measurement of factor VII (FVTI) coagulant activity will be presumably requested to clinical laboratories with increasing frequency. To assess the influence on FVII assays of different thromboplastins and FVII-deficient plasmas we compared performances of all possible combinations of 5 thromboplastins and 6 deficient plasmas. The reproducibility of the clotting times of the dose-response curves for human and rabbit thromboplastins were acceptable (CV lower than 7%), whereas bovine thromboplastin had a higher CV. Reproducibility was very similar for all deficient plasmas when they were used in combination with a given thromboplastin. Responsiveness of the dose-response curve did not depend on the deficient plasma but rather on the thromboplastin: one rabbit thromboplastin was the least responsive, the bovine thromboplastin the most responsive, the human and the remaining two rabbit thromboplastins had intermediate responsiveness. Assay sensitivity to cold-activated FVII varied according to the thromboplastin: the bovine thromboplastin was the most sensitive, the human thromboplastin the least sensitive, of the three rabbit thromboplastins two were relatively sensitive, one was almost insensitive. In conclusion, our results indicate that thromboplastin rather than deficient plasma is the crucial factor in the standardization of FVII assay.


1967 ◽  
Vol 56 (4) ◽  
pp. 619-625 ◽  
Author(s):  
Hans Jacob Koed ◽  
Christian Hamburger

ABSTRACT Comparison of the dose-response curves for LH of ovine origin (NIH-LH-S8) and of human origin (IRP-HMG-2) using the OAAD test showed a small, though statistically significant difference, the dose-response curve for LH of human origin being a little flatter. Two standard curves for ovine LH obtained with 14 months' interval, were parallel but at different levels of ovarian ascorbic acid. When the mean ascorbic acid depletions were calculated as percentages of the control levels, the two curves for NIH-LH-S8 were identical. The use of standards of human origin in the OAAD test for LH activity of human preparations is recommended.


1981 ◽  
Vol 27 (11) ◽  
pp. 1838-1844 ◽  
Author(s):  
G A Hudson ◽  
R F Ritchie ◽  
J E Haddow

Abstract Antiserum performance in a nephelometric system can be characterized by parameters derived from measuring reaction rates. The characterization process is derived from a series of dose-response curves (elicited nephelometric response vs antigen concentration) generated from various dilutions of the antiserum being tested. Antiserum titer can then be calculated by plotting the antigen concentration found at one-half the maximum nephelometric response (Hmax) of each dose-response curve (C50) vs the corresponding antiserum dilution. Antiserum avidity can be calculated by plotting Hmax against its corresponding antiserum concentration. After general expressions are determined for C50 and Hmax vs antiserum concentration, a single dose-response curve suffices for characterizing antisera with respect to titer and avidity. Direct evidence is provided for the validity of C50 and Hmax as measures of titer and avidity by correlating these parameters with antiserum binding strength and with the number of antibodies eluted from immobilized antigen. This method can be applied to evaluate and compare different antiserum lots having the same specificity, to identify reagent inadequacies by comparing antisera of different specificity, and to predict the optimal antiserum dilution to use in performing an assay.


1991 ◽  
Vol 18 (10) ◽  
pp. 1873-1876 ◽  
Author(s):  
Douglas L. Westphal ◽  
Owen B. Toon

2009 ◽  
pp. 55-62
Author(s):  
Fabrizio Rizzi

- This article recounts the doubts and fears of an experienced analyst who is now an apprentice acrobat. He is forced to keep himself tiredly balanced between psychological and physical limits imposed by age, restrictions introduced by the National Health System and categories of patients who have precedence over others. He cannot receive all of the patients who ask for him and even those who he does receive will have to be discharged in the short term. Explaining to them, with intellectual honesty, that the community service has rules that limit his wishes as well. What can this be if not acrobatics? In the end it means combining the contradictory but perhaps also the most real aspects of life itself. [KEY WORDS: desires, personal and environmental limits, truth of the therapist]


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