SOLUBLE NUTRIENTS IN PROTEIN SUPPLEMENTS AND IN SITU DISAPPEARANCE

1985 ◽  
Vol 65 (3) ◽  
pp. 667-672 ◽  
Author(s):  
J. R. BARRIO ◽  
F. N. OWENS ◽  
A. L. GOETSCH

The effect of removal of soluble nutrients from soybean meal (SBM) and meat meal (MM) on in situ disappearance of dry matter (DMD) and nitrogen (ND) was measured. All feeds were rinsed with pH 6 buffer (R) without or with subsequent extraction with buffer at pH 5 (5) or pH 7 (7). Feeds were incubated in the rumen of heifers fed diets containing 20 or 80% concentrate (20%C and 80%C, respectively). Ruminal pH was higher (P < 0.05) in heifers fed the 20%C diet than in animals receiving the 80%C ration, while ruminal ammonia concentration tended to be greater with the 80%C diet. In situ DMD and ND were linearly related to incubation time (P < 0.01) with little indication of a quadratic effect of time. In situ ND of feeds extracted at pH 7 was greater (P < 0.05) than ND of feeds extracted at pH 5, but no interaction of diet and extraction pH or feed and extraction pH was apparent. Hence, altered protein solubility due to varied ruminal pH would not appear to be responsible for differences in ND with the different diets. In situ DMD and ND of both feedstuffs were greater (P < 0.01) with the 20%C than the 80%C diet. Hence, differences in microbial types or activities rather than chemical characteristics of the substrate are probably responsible for greater proetein loss with the lower concentrate diet. Key words: In situ, dry matter disappearance, nitrogen disappearance, soybean meal, meat meal

1988 ◽  
Vol 39 (4) ◽  
pp. 721 ◽  
Author(s):  
J Leibholz ◽  
Y Mollah

Six male Landrace x Large White pigs (25 kg liveweight) were fitted with T-shape cannulae in the terminal ileum. The pigs were fed 1.2 kg dry matter per day containing 4.5 g threonine/kg of diet from continuous belt feeders. The apparent digestibility of dry matter (DM) to the terminal ileum was 0.91, 0.87, 0.85, 0.81, 0.80 and 0.75 for pigs given diets containing milk, fish meal, soybean meal, meat meal, sunflower meal and cotton seed meal respectively. The apparent digestibility of nitrogen (N) to the terminal ileum was 0.87, 0.87, 0.86, 0.86, 0.81 and 0.74 for pigs given diets containing milk, fish meal, soybean meal, sunflower meal, meat meal and cottonseed meal, respectively. The true digestibility of threonine to the terminal ileum was 0.96, 0.95, 0.90, 0.87, 0.80 and 0.66 for the diets containing milk, fish meal, sunflower meal, soybean meal, meat meal and cottonseed meal, respectively.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 220-220
Author(s):  
Cody Ream ◽  
Allison V Stevens ◽  
Gwinyai Chibisa

Abstract This study examined the effects of altering ruminal fermentable carbohydrate (RFC) supply by feeding different amounts of corn and wheat in finishing cattle diets containing 15% corn dried distillers grains [DDGS; dry matter (DM) basis] on ruminal fermentation characteristics and nitrogen (N) utilization. Six ruminally-cannulated crossbred heifers were used in a replicated 3 × 3 Latin square design with 28 d periods. Dietary treatments were either corn (73% of diet DM; CON), 53:20 corn:wheat blend (20W) or 33:40 corn:wheat blend (40W) as the major fermentable carbohydrate source. Diets were isonitrogenous (12% CP; DM basis). Feed intake was measured daily. Indwelling pH logger were used to measure ruminal pH (d 22 to 28) and ruminal fluid was collected from d 26 to 28 to determine fermentation characteristics, as were feces and urine to measure N excretion. Data was analyzed using PROC MIXED in SAS. Dry matter and N intake tended to be lower (P ≤ 0.07) for heifers fed the 40W compared to the CON and 20W diets. There was no diet effect (P = 0.15) on total VFA concentration; however, the duration and area for pH &lt; 5.5 tended to be longer (P = 0.07) and greater (P = 0.096), respectively, for heifers fed the 20W and 40W compared to the CON diet. There was no diet effect (P ≥ 0.44) on ruminal ammonia concentration and total urine N excretion (g and % of N intake). However, urine urea N (UUN) excretion as a percentage of total urine N tended to be lower (P = 0.05) in heifers fed the 20W and 40W compared to the CON diet. In summary, although altering RFC supply by feeding increasing amounts of wheat resulted in a decrease in UUN excretion, it also caused a decrease in ruminal pH that possibly compromised DM intake.


1980 ◽  
Vol 31 (3) ◽  
pp. 627 ◽  
Author(s):  
AR Alimon ◽  
DJ Farrell

Quantitative estimates of disappearances of dry matter, nitrogen and amino acids anterior to the mid-point and terminal ileumof the small intestine were made in pigs prepared with re-entrant cannulas and offered six diets containing wheat alone or supplemented with either meat meal, fish meal, peanut meal, soybean meal or sunflower meal. Apparent digestibilities of dietary dry matter and nitrogen were also estimated by faecal measurements. Disappearance of dry matter and nitrogen was greater anterior to the mid-point than at the end of the small intestine and the rectum. Amounts disappearing at each location and between the three locations differed significantly between protein sources. Up to 10% of dietary dry matter and nitrogen disappeared in the large intestine. For lysine, methionine, threonine and valine, absorption was greater anterior than posterior to the midpoint of the small intestine for all diets except that containing only wheat. Measurements made of the disappearance of amino acids anterior to the large intestine indicated that the apparent availability of the majority of ammo acids of peanut meal, followed by soybean meal, was greater than those of meat meal (52 % crude protein) and fish meal (50 % crude protein).


1981 ◽  
Vol 61 (2) ◽  
pp. 453-461 ◽  
Author(s):  
J. M. BELL ◽  
D. M. ANDERSON ◽  
A. SHIRES

Two experiments were conducted to evaluate rapeseed meal (RSM) produced from seed of Brassica campestris L. ’Candle’ in comparison with B. napus L. ’Tower’, soybean meal and fababeans (Vicia faba) as protein supplements for growing swine. In experiment 1 Candle RSM (5, 10 or 15%) was substituted for soybean meal or fababeans in barley- and wheat-based diets for 120 pigs from 23 to 88 kg liveweight. The effects of supplementary iodine (0.14 mg I/kg diet) and amino acids (0.15% lysine and 0.05% methionine) on the nutritive value of Candle RSM were also studied. The results of experiment 1 indicated that daily feed intake and carcass value index were not significantly (P > 0.05) affected by diets. The growth and feed utilization responses of pigs fed diets which contained soybean meal and fababeans as the sole source of supplementary protein were similar and the replacement of these protein supplements with Candle RSM, unless supplemented with amino acids, resulted in a reduction in rate of growth and efficiency of feed utilization. Gains adjusted by regression for feed intake variations showed benefit from lysine. Diets containing 15 % of either Candle or Tower RSM, plus lysine, were utilized about 95% as efficiently as soybean meal diets. Supplementation with iodine produced no significant response. The digestibility coefficients of the protein and energy of Candle RSM and soybean meal were determined in experiment 2. The apparent digestibility of protein in Candle RSM was 81% and energy digestibility was 71%. The digestible energy content was 14.12 MJ/kg (3370 kcal/kg), dry matter basis.


2021 ◽  
Vol 42 (2) ◽  
pp. 795-808
Author(s):  
Ernestina Ribeiro Santos Neta ◽  
◽  
Daiany Iris Gomes ◽  
Luis Rennan Sampaio Oliveira ◽  
Rafael Mezzomo ◽  
...  

This study evaluated the use of babassu cake as a replacement for soybean meal and pineapple byproduct silage as roughage in the diets of feedlot sheep. Ten rumen-fistulated sheep were used and distributed in an experimental design consisting of two 5 x 5 Latin squares. Babassu cake replaced soybean meal at four concentration levels (0, 26.66, 53.33, and 80%), and pineapple byproduct silage was used as roughage. A treatment was also evaluated in which elephant grass silage was used as roughage and corn and soybean meal were used as the concentrate. No effect of babassu cake as a replacement for soybean meal on dry matter intake, neutral detergent fiber, or crude protein was observed. Higher babassu cake replacement levels in the diets resulted in linear reductions in the digestibility of the dry matter, neutral detergent fiber, organic matter, nonfiber carbohydrates and total carbohydrates. For the digestibility of the crude protein, no effect was observed after the addition of babassu cake. The replacement levels of the babassu cake had no effect on the intake and retention of the daily nitrogen, ammonia nitrogen and ruminal pH. Babassu cake could replace soybean meal at concentrations of up to 80% when the roughage source was pineapple byproduct silage. Compared with elephant grass silage, pineapple byproduct silage improves nutrient intake and digestibility and nitrogen intake, retention, and absorption without compromising the ruminal pH or ammonia nitrogen of feedlot sheep.


2020 ◽  
Vol 100 (4) ◽  
pp. 716-728
Author(s):  
A.A. Pursley ◽  
B. Biligetu ◽  
T.D. Warkentin ◽  
H.A. Lardner ◽  
G.B. Penner

The objective of this study was to evaluate the inclusion rate of pea hay in barley or oat hay diets for beef cattle. Six ruminally cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square (25 d periods) with a 2 × 3 factorial design. Treatments included whole-crop barley or oat hay with pea hay blended in to achieve inclusion rates of 0%, 15%, or 30% (dry matter basis) of the forage. Pea hay inclusion increased dry matter intake (DMI; P = 0.03) by 0.75 kg d−1 relative to diets without pea hay, but the response was not linear or quadratic. Inclusion of pea hay linearly increased mean ruminal pH (P = 0.039), the concentration of butyrate in ruminal fluid (P = 0.013), plasma urea nitrogen (N) concentration (P = 0.001), and quadratically increased ruminal ammonia concentration (P < 0.001). Pea hay inclusion reduced crude protein (CP) digestibility by 2.87% relative to cereal-only treatments (P = 0.025), but did not affect N intake, microbial N, or N excretion. Overall, pea hay inclusion increased DMI, increased ruminal butyrate concentration, but reduced CP digestibility without affecting N balance.


1995 ◽  
Vol 75 (3) ◽  
pp. 371-377 ◽  
Author(s):  
K. Stanford ◽  
T. A. McAllister ◽  
Z. Xu ◽  
K.-J. Cheng ◽  
M. Pickard

An experiment was undertaken to determine the effect of lignosulfonate (LSO3) treatment on the rumen-undegradable protein (RUDP) value of canola meal (CM) and soybean meal (SM). Canola meal and SM were treated with 7% LSO3 and heated (95 °C) for 1 h. Treated and untreated meals were compared in vitro, in situ and in digestibility and growth experiments using Romanov × Suffolk and Romanov × Dorset lambs. Lambs were fed four barley-based diets [13.5% crude protein (CP)] with one of CM, SM, LSO3-treated CM (LSO3-CM) or LSO3-treated SM (LSO3-SM) as protein supplements. Lignosulfonate treatment increased acid detergent insoluble nitrogen (ADIN) five-fold in SM and threefold in CM. After 6 h incubation with ruminal fluid, ammonia production from LSO3-treated meals was one-half that from untreated meals. Lignosulfonate treatment reduced (P < 0.01) in situ rates of dry matter (DM) and protein disappearance and the soluble DM and protein fractions for both SM and CM, but not the potentially digestible fractions. No differences in feed conversion or average daily gain (P > 0.05) were found among the treatments. Although LSO3 treatment did not improve the ADG of lambs fed barley-based diets, it increased the RUDP value of SM and CM without adverse effects on digestibility. Key words: Lignosulfonate, canola meal, soybean meal, lamb


1990 ◽  
Vol 6 (1) ◽  
pp. 1-4 ◽  
Author(s):  
L.S. Sticker ◽  
F.G. Hembry ◽  
T.W. White ◽  
H.W. Essimg ◽  
Larry D. Guthrie
Keyword(s):  

1994 ◽  
Vol 74 (3) ◽  
pp. 479-485 ◽  
Author(s):  
G. R. Khorasani ◽  
J. J. Kennelly ◽  
P. H. Robinson

The objectives of the present study were to evaluate the nutritive value of expeller linseed meal (ELM) and solvent linseed meal (SLM) as protein supplements for lactating dairy cows utilizing analytical and in situ procedures, and determine the in vivo nutritive value of the most promising linseed meal in diets containing either barley or corn-based concentrate. Canola meal (CM) and soybean meal (SBM) were included in the evaluative stage for comparative purposes. Linseed meals contained 37.8 and 35.4% crude protein (CP); 22.6 and 25.9% neutral detergent fibre (NDF); and 12.9 and 7.1% crude fat for ELM and SLM, respectively. Rate and extent of dry matter, NDF, and CP digestion, as determined by the rumen in situ technique, indicated that ruminal digestibility of SLM was similar to that observed for CM, but ruminal escape of ELM protein was higher than for CM. The proportion of ruminal escape protein was similar for SLM, CM and SBM. Total tract CP digestibilities of SLM and CM were similar to and lower than those observed for ELM and SBM. Soybean meal had the highest total tract CP digestibility. Based on results of the analytical and in situ procedures, SLM was selected for evaluation in the in vivo experiment. Results indicated that SLM did not have any deleterious effect on digestion of other components of the diet, and parameters of rumen fermentation indicated similar digestion patterns for cows fed SLM and CM. Overall, results indicate that SLM has potential as a replacement for CM or SBM in diets of dairy cattle. It was also concluded that replacement of barley with corn does not influence the nutritive value of SLM for dairy cows. Key words: Linseed meal, canola meal, dairy cattle


2020 ◽  
Vol 42 ◽  
pp. e48586
Author(s):  
Tamara Tais Tres ◽  
Clóves Cabreira Jobim ◽  
Tatiana Garcia Diaz ◽  
João Luiz Pratti Daniel ◽  
Fernando Alberto Jacovaci

The experiment was carried out to evaluate total and partial digestibility of nutrients, the efficiency of microbial protein synthesis, in situ degradability and ruminal parameters in cattle fed diets with rehydrated corn grain silages, okara or soybean grain. Three Holstein steers were distributed in a 3 x 3 Latin square design. The treatments evaluated were: SO (corn grain silage + 30% okara), SSG (corn grain silage + 20% soybean grain) and CG (dry corn grains). The ruminal digestibility of non-fiber carbohydrates (NFC) increased in SO (88.34%) and SSG treatments (87.87%), compared to the CG treatment (63.48%). The minimum ruminal pH value was 6.01, observed 4.13 hours after feeding a diet with SO. The highest ammonia-N contents were 15.25 and 15.07 mg dL-1 observed in SSG and SO, respectively, 2.45 and 2.61 hours after feeding. Treatments SO and SSG showed higher fraction A content (readily degradable fraction) and C (constant rate of degradability of fraction B). The effective degradability (ED) of dry matter (DM) was higher for the diets SO and DE of CP was higher for treatments SO and SSG. SSG and SO result in better utilization of nutrients by animals.


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