STUDIES ON THE PERFORMANCE, DEVELOPMENT AND CARCASS COMPOSITION OF THE GROWING PIG: EFFECTS OF SEX, FEEDING REGIME AND AGE ON BLOOD SERUM PARAMETERS

1983 ◽  
Vol 63 (4) ◽  
pp. 977-984 ◽  
Author(s):  
H. DOORNENBAL ◽  
A. K. W. TONG ◽  
A. H. MARTIN ◽  
A. P. SATHER
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Age Groups ◽  
Inorganic Phosphorus ◽  
Carcass Composition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Parameters ◽  
Feeding Regime

Blood samples from 120 pigs serially slaughtered at five age groups, starting at 85 days of age with intervals of 2 wk, were used to study the effects of sex, feeding regime and age on a 12 component metabolic blood serum profile. The differences between barrows and gilts were small and only significant (P < 0.05) for blood urea. Pigs fed ad libitum had a significantly (P < 0.05) higher level of blood urea, calcium, inorganic phosphorus and alkaline phosphatase but a significantly (P < 0.01) lower level of creatinine than restricted fed pigs. Age had the most pronounced effects on the serum parameters studied, with blood urea, calcium, bilirubin, creatinine, total serum protein and albumin showing a significant (P < 0.01) increase per unit of serum with increasing age. The serum glucose, inorganic phosphorus, glutamic oxaloacetic transaminase and lactate dehydrogenase were also significantly (P < 0.01, P < 0.05 for glucose) influenced by age but did not show a consistent trend across the age groups. Serum alkaline phosphatase was the only component which significantly (P < 0.01) decreased with age. The results indicate that when these serum components are to be used for diagnostic or comparison purposes, age adjustment must be applied. Key words: Blood parameters, pigs, age, feeding regime, sex

scholarly journals Variability of blood serum biochemical parameters in Karakachan sheep

2015 ◽  
Vol 31 (1) ◽  
pp. 55-62 ◽  
Author(s):  
O. Stevanovic ◽  
M. Stojiljkovic ◽  
D. Nedic ◽  
D. Radoja ◽  
V. Nikolic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Age Groups ◽  
Balkan Peninsula ◽  
Inorganic Phosphorus ◽  
Sheep Breed ◽  
Mean Values ◽  
Indigenous Sheep ◽  
Significant Difference ◽  
Glutamyl Transferase

Karakachan sheep represents an endangered, indigenous sheep breed from Balkan Peninsula. There is relatively little information about the characteristics of this sheep breed in the veterinary scientific literature. The aim of this research was an examination of certain metabolic profile parameters of the Karakachan sheep blood serum, and variability of their concentrations in comparison to age and some other indigenous sheep breeds from Balkans. Examination was conducted on 14 clinically healthy sheep divided in two age groups. Blood samples were collected by puncture of v. jugularis and blood serum was separated after spontaneous coagulation. The concentrations of total protein, albumin, calcium, inorganic phosphorus, aspartate amino transferase (AST) and ?- glutamyl transferase (GGT) were determined. In relation to age of Karakachan sheep, statistically significant difference between the calculated mean values of examined parameters was not observed. A statistically significant difference was found between the mean concentrations of the studied parameters in Karakachan sheep and other breeds in total protein (Tsigai, Dubrovnik and Dalmatian sheep), albumin (Dalmatian), calcium and inorganic phosphorus (Tsigai, Dubrovnik) and AST's (Dalmatian, Karakachan sheep from Bulgaria).

Precipitation of serum proteins by the lectin from wheat-germ.

1987 ◽  
Vol 33 (1) ◽  
pp. 185-186 ◽  
Author(s):  
W E Schreiber ◽  
L Whitta
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Protein ◽  
Wheat Germ ◽  
Serum Proteins ◽  
Total Serum Protein ◽  
Total Serum ◽  
Wheat Germ Lectin

Abstract We investigated the composition of the precipitate that forms when wheat-germ lectin derived from Triticum vulgaris is added to serum. A number of serum proteins are precipitated, representing about 2.5% of the total serum protein. This study demonstrates that the interaction of this lectin with the bone isoenzyme of alkaline phosphatase is not specific.

Characterization of placental isoenzyme of alkaline phosphatase in human pregnancy serum

1969 ◽  
Vol 47 (2) ◽  
pp. 147-155 ◽  
Author(s):  
N. K. Ghosh ◽  
W. H. Fishman
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Alkaline Phosphatase ◽  
Heat Stability ◽  
Biochemical Properties ◽  
Heat Inactivation ◽  
Total Serum ◽  
Placental Alkaline Phosphatase ◽  
Human Placental Alkaline Phosphatase ◽  
Alkaline Phosphatase Isoenzyme ◽  
In Pregnancy

Human placental alkaline phosphatase isoenzyme has been characterized in pregnancy serum by several biochemical criteria. The total serum alkaline phosphatase, its L-phenylalanine-sensitive moiety, heat inactivation, and the ratio of enzyme activity at pH 10.7 versus 9.8 (10.7/9.8 R) were measured during parturition and 59 weeks of pre- and post-natal periods. The extent of L-phenylalanine inhibition, heat stability, and 10.7/9.8 R of serum alkaline phosphatase progressively increased during gestation attaining maximum values during the delivery, after which they gradually declined. The electrophoretic behaviors of alkaline phosphatase isoenzymes of pregnancy sera were followed by starch- and Sephadex-gel electrophoreses. Alkaline phosphatase has been purified 300-fold from the placenta of the subject whose serum enzyme was investigated. The biochemical properties, including the electrophoretic behavior and neuraminidase sensitivity of heat-stable alkaline phosphastase in pregnancy sera at term, were comparable to those of purified placental alkaline phosphatase. The values for 10.7/9.8 R of the pregnancy sera were statistically different from those of sera from normal nonpregnant women. The results obtained in this study suggest that the enhanced level of pregnancy serum alkaline phosphatase is due to the enrichment of the circulation with an isoenzyme of placental origin.

The activity of alkaline phosphatase in the blood of purebred and crossbred heifers

2020 ◽  
pp. 13-20
Author(s):  
R. Milushev ◽  
V. Epifanov
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Live Weight ◽  
Milk Productivity ◽  
Holstein Breed ◽  
Lactating Cows ◽  
Adaptive Capabilities ◽  
Age Related ◽  
Productive Traits

Simmental breed of cattle combines dairy and beef productivity. The crossbreeding cows of Simmental breed with Red-and-White Holstein breed of cattle was supposed to obtain animals with high milk productivity, good adaptability to the industrial technology of milk productivity. Red-and-White breed of cows has been bred in Russia as a result of this crossbreeding. It is a relatively young breed of dairy productivity in our country. In order to evaluate the effect of crossbreeding and study the adaptive capabilities obtained by animals, the synthesis of alkaline phosphatase by its activity in blood serum has been studied. The level of activity of the enzyme reflected the intensity and direction of biochemical processes in animals, affecting the state of homeostasis in purebred (Simmental) and crossbred (F1 Simmental×Red-and-White Holstein) heifers and first-calf heifers. It has been found that activity of serum alkaline phosphatase has decreased with age in both purebred and crossbred animals. Crossbreds F1 surpassed purebred heifers in enzyme activity at 1 and 10 months of age, this difference was significant. The activity of the enzyme in first-calf heifers was different from that in the blood of growing heifers, they had it lower. A close and significant correlation has been found between the live weight of growing crossbred heifers and the activity of alkaline phosphatase in the blood serum at 2-, 6- and 12-month age. Crossbred first-calf heifers were significantly higher in milk yield for 90 days of lactation than their Simmental herdmates. The set of data on the activity of alkaline phosphatase in the blood of heifers and lactating cows is of both theoretical and practical value for zootechnical and biological sciences, revealing the regularities of the heterosis effect, deepening knowledge about the age-related biochemistry of crossbred animals and the formation of productive traits.

Bone metabolism biomarkers in men with advanced prostate cancer (PC) and bone metastases treated with denosumab versus zoledronic acid (ZA).

2015 ◽  
Vol 33 (7_suppl) ◽  
pp. 267-267
Author(s):  
Matthew Raymond Smith ◽  
Janet Elizabeth Brown ◽  
Karim Fizazi ◽  
Laurence Klotz ◽  
Gavin M. Marx ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Treatment Group ◽  
Bone Metastases ◽  
Bone Metabolism ◽  
Proportional Hazards Model ◽  
Serum Alkaline Phosphatase ◽  
Cox Proportional Hazards Model ◽  
Total Serum ◽  
Double Blind ◽  
Increased Risk

267 Background: Denosumab, compared with ZA, has recently demonstrated significant benefits in preventing skeletal-related events (SREs) in a double-blind phase 3 study of men with castration-resistant PC and bone metastases (N=1901). This post hoc analysis was performed to determine if key bone metabolism biomarker levels correlated with time-to-first SRE. Methods: Time-to-Grade ≥2 increase in total serum alkaline phosphatase (ALP) was assessed for correlation with time-to-first SRE. Levels of urinary N-telopeptide (uNTX) and serum bone-specific alkaline phosphatase (BSAP) markers were measured at baseline and study week 13, and correlations with time-to-first SRE were assessed. Covariate analyses were performed using a Cox proportional hazards model, stratified by treatment group or (for baseline analyses) with treatment group as the independent variable. Results: Analysis of the relationship between time-to-Grade ≥2 increase in total ALP and time-to-first SRE demonstrated that Grade ≥2 increases in ALP of PC patients were associated with a higher risk of first SRE (hazard ratio [HR] 1.838, 95% confidence interval [CI] 1.559, 2.167; P<0.0001). In dichotomized analyses (< vs ≥ median), a higher level of uNTX and BSAP (at baseline or week 13) was correlated with an increased risk of first SRE (Table). In baseline covariate analyses, treatment benefit for denosumab was maintained after adjusting for either baseline uNTX (HR 0.818, 95% CI 0.703, 0.951; P=0.0091) or BSAP (HR 0.813, 95% CI 0.700, 0.943; P=0.0061). Conclusions: Higher levels of uNTX or BSAP at baseline or week 13 were associated with worse SRE outcomes in men with advanced PC and bone metastases. Denosumab was more efficacious for preventing or delaying SREs compared with ZA, regardless of bone-related biomarker levels. Relationship of covariate to time-to-first SRE Clinical trial information: NCT00321620. [Table: see text]

Activity of alkaline phosphatase in male lamb blood serum in relation to age and growth rate

2021 ◽  
pp. 9-16
Author(s):  
Y. Zharikov ◽  
L. Kaneva
Keyword(s):  
Growth Rate ◽  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Serum Alkaline Phosphatase ◽  
Live Weight ◽  
Growth Potential ◽  
Age And Growth ◽  
Serum Alkaline Phosphatase Activity ◽  
Growth Intensity ◽  
Lower Productivity

Purpose: to assess the correlation of serum alkaline phosphatase with the live weight and growth rate of male lambs during the milk and post-milk growing periods.Materials and methods. The study of indicators of serum alkaline phosphatase activity in 74 male lambs aged one and five months was carried out in connection with the intensity of growth and the peculiarities of metabolism. All the male lambs were born at the same time and were raised for up to six months under similar feeding and maintenance conditions. To study the dynamics of changes in the live weight of young animals, individual weighing was carried out monthly with an accuracy of 0.1 kg. Based on the results of weighing, the absolute, relative, and average daily live weight gains were calculated and the animals were divided into three groups. The analysis of the serum for the content of the studied parameters was performed by photometry with the help of the corresponding sets of reagents of the company «VitalDiagnostics SPb».Results. During the observation period, the average increase in live weight of male lambs was 21,14 kg, or 136,75 g per day. At one month of age, the activity of alkaline phosphatase was 188,61±9.50 U/l, and at five months — 100,01±3,58, or 47% (P<0,001) lower. It was found that the activity of the enzyme during the suckling period was positively associated with live weight and growth intensity (r=0,35 and 0,49), and after weaning from mothers — negatively (r = -0,46 and -0,46, respectively). The activity of alkaline phosphatase was consistently increased in monthly male lamb when they moved from a group with lower productivity to a larger one — 160,5 — 191,4 — 214,9, and in five-month-olds, on the contrary, decreased — 121,0 — 98,1 — 80,2 E/l. At the age of one month, the increase in growth intensity was accompanied by an increase in the blood serum of male lamb values of gamma-glutamyltranspeptidase, glucose, calcium and phosphorus, and a decrease in urea and cholesterol levels. At the age of five months, both the list of links and their vector changed. In the serum of fast-growing animals, the de Ritis coefficient and gamma-glutamyltranspeptidase were higher, but the activity of lactate dehydrogenase and creatine phosphokinase was lower.Conclusion. The presence of a correlation between alkaline phosphatase and growth makes it possible to use alkaline phosphatase in zootechnical practice and breeding work as an additional criterion for the growth potential of male lambs up to six months in the early stages of ontogenesis. However, the change in the nature of the relationship from plus to minus implies a differentiated approach in the use of this indicator, taking into account the age of the male lambs.

Electrophoretic Separation of Alkaline Phosphatase Isoenzymes: A Clinical Evaluation

1972 ◽  
Vol 17 (5) ◽  
pp. 172-175 ◽  
Author(s):  
R. R. G. Warwick ◽  
D. J. C. Shearman ◽  
I. W. Percy-Robb ◽  
A. F. Smith
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Electrophoresis ◽  
Serum Alkaline Phosphatase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Total Serum ◽  
Electrophoretic Separation ◽  
Nucleotidase Activity ◽  
Total Alkaline Phosphatase ◽  
Alkaline Phosphatase Isoenzymes ◽  
Total Alkaline

In 40 patients with a raised total serum alkaline phosphatase, polyacrylamide gel electrophoresis was used to separate the liver, bone and intestinal isoenzymes of alkaline phosphatase. The method confirmed the source of the raised alkaline phosphatase in patients with established bone or liver disease. The technique was found to be more reliable than the estimation of 5'-nucleotidase activity in the detection of a raised alkaline phosphatase of hepatic origin and was also useful when the raised total alkaline phosphatase came from two sources.

Biochemical Changes following Parathyroidectomy

1993 ◽  
Vol 16 (10) ◽  
pp. 700-703 ◽  
Author(s):  
P.K.G. Chandran ◽  
T.J. Ulahannan ◽  
M. Skiles
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Calcium ◽  
Serum Alkaline Phosphatase ◽  
Inorganic Phosphorus ◽  
Biochemical Changes ◽  
Postoperative Hypocalcemia ◽  
Subtotal Parathyroidectomy ◽  
Osteoblastic Activity ◽  
End Stage ◽  
Phosphorus Levels

Biochemical changes that had appeared after subtotal parathyroidectomy (PTx) in 26 patients with end-stage renal failure were observed. The volume of excised parathyroid glands was also measured. Serum calcium and inorganic phosphorus levels fell after PTx; only to rise in due course. Serum alkaline phosphatase levels rose after PTx, reaching a peak by the 14th postoperative day. These elevated levels returned to normal range at about three months after PTx. Strong correlation was noted among the degree of postoperative hypocalcemia, and increase in serum alkaline phosphatase, but not between absolute pre or postoperative alkaline phosphatase levels and changes in serum calcium or phosphorus concentrations. Nevertheless, significant correlation was seen between pre-PTx levels of alkaline phosphatase and its short-lived postoperative rise, indicating hastened osteoblastic activity.

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