THE EFFECTS OF FEEDING COUMESTROL ON THE REPRODUCTIVE ORGANS OF PREPUBERTAL LAMBS

1980 ◽  
Vol 60 (1) ◽  
pp. 53-58 ◽  
Author(s):  
F. E. NEWSOME ◽  
W. D. KITTS
Keyword(s):  
Estrogen Receptors ◽  
Follicular Development ◽  
Reproductive Organs ◽  
Binding Affinities ◽  
Uterine Weight ◽  
Endometrial Cell ◽  
Ovarian Weight ◽  
Ewe Lambs ◽  
Relative Binding

Coumestrol and estradiol were fed to prepubertal ewe lambs for 12 days. The level of coumestrol fed relative to estradiol was estimated from the relative binding affinities of the uterine cytosol estrogen receptors to be the biologically effective amount. The effects of coumestrol treatment were increased uterine weight and decreased ovarian weight and follicular development. There was a relationship between uterine weight and exchanged tritiated estradiol bound to the endometrial cell nuclear pellet.

OVULATION RATE AND WEIGHT OF THE REPRODUCTIVE ORGANS OF YORKSHIRE AND LACOMBE SWINE

1971 ◽  
Vol 51 (1) ◽  
pp. 141-146 ◽  
Author(s):  
G. W. DYCK
Keyword(s):  
Reproductive Organs ◽  
Ovulation Rate ◽  
Uterine Weight ◽  
Ovarian Weight

Ovulation rates were determined in 41 Yorkshire, 46 Lacombe and 26 Lacombe × Yorkshire gilts, and in 37 Yorkshire and 32 Lacombe primiparous sows. Ovulation rates in gilts remained constant from puberty to the fifth estrus. For Yorkshire, Lacombe and crossbred gilts the ovulation rates were 12.2, 14.4 and 14.0, respectively. The ovulation rates in Yorkshire and Lacombe sows were the same (17.0). In sows, ovulation rate increased significantly from the first to second post-weaning estrus (15.4 vs. 17.5). In Yorkshire gilts, ovarian and uterine weights were significantly less than in Lacombe gilts. Within breeds, ovarian weight remained similar while uterine weight increased (P < 0.05) from the first to second estrus. In sows, ovarian weight, which was similar for both breeds, increased significantly from the first to second post-weaning estrus. Uterine weight in Lacombe sows was significantly greater than in Yorkshire sows, and increased (P < 0.05) from the first to third post-weaning estrus. In Yorkshire sows uterine weight remained constant over the first three estrous periods after weaning.

COMPARISON OF RELATIVE BINDING AFFINITIES OF ENDOCRINE ACTIVE COMPOUNDS TO FATHEAD MINNOW AND RAINBOW TROUT ESTROGEN RECEPTORS

2005 ◽  
Vol 24 (11) ◽  
pp. 2948 ◽  
Author(s):  
Jeffrey S. Denny ◽  
Mark A. Tapper ◽  
Patricia K. Schmieder ◽  
Michael W. Hornung ◽  
Kathleen M. Jensen ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Estrogen Receptors ◽  
Fathead Minnow ◽  
Binding Affinities ◽  
Active Compounds ◽  
Relative Binding

The biologic activity of selenoestrogens

1982 ◽  
Vol 60 (2) ◽  
pp. 152-156 ◽  
Author(s):  
G. Longcope ◽  
T. Arunachalam ◽  
E. Caspi
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptors ◽  
Uterine Weight ◽  
Binding Properties ◽  
Active Compounds ◽  
Relative Binding ◽  
Biologic Activity ◽  
Differential Imaging

For differential imaging of mammary tumors with estrogen receptors and without estrogen receptors we required γ-emitting estrogen analogues. In this paper we report on the binding properties of 7α-, 16α-, and 17α-methylselenoestrogens and 17α-phenylselenoestrogens relative to the binding properties of estradiol.The selenium-containing estrogens retained the ability to displace [3H]estradiol from the estrogen receptor of rabbit uterine cytosol, although in most instances the displacement was small (3–7% compared to estradiol).The most active compounds were 16α-phenylselenoestrone, 16α-methylselenoestradiol, and 17α-methylselenomethyl-estradiol which had relative binding of 23, 27, and 31%, respectively, compared with that of estradiol.16α-Methylselenoestradiol was able to translocate the estrogen cytosol receptor to the nucleus, in vitro, but was not able to increase the uterine weight when administered to mice in vivo.

scholarly journals Organometric data of the reproductive tract in cycling and early pregnant Hungarian Mangalica pigs

2004 ◽  
Vol 47 (6) ◽  
pp. 585-594 ◽  
Author(s):  
K.-P. Brüssow ◽  
I. Egerszegi ◽  
J. Rátky ◽  
F. Soós ◽  
P. Garcia Casado ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Reproductive Organs ◽  
Corpora Lutea ◽  
Estrus Synchronization ◽  
Uterine Weight ◽  
Ovarian Weight ◽  
Embryo Recovery ◽  
Study Support ◽  
Left And Right ◽  
Initial Process

Abstract. Since the morphology of the reproductive organs could be a wherefore of reproductive performance of Mangalica, this study was conducted to get some more information about the number of corpora lutea and the size of reproductive tract both in cycling and pregnant Mangalica pigs. Two experiments were carried out to investigate the reproductive tract of Mangalica gilts. In Experiment 1, reproductive organs of 66 gilts (12–15 month of age) were recovered immediately after slaughter to determine the number of ovarian features, the weight and diameter of the ovaries, and the weight and length of oviducts and uteri, respectively. In Experiment 2, 22 puberal Mangalica (M) gilts and 34 German Landrace (L) gilts were used following estrus synchronization and artificial insemination. Genital tracts were recovered by ovario-hysterectomy on day 1 (n = 8 M; 10 L), day 12 (n = 8 M; 22 L) and day 24 (n = 6 M; 2 L) of pregnancy and the number of corpora lutea, ovarian weight and diameter, and the weight and length of oviducts and uteri evaluated. Oviducts and uterine horns (day 1 and 12, respectively) were flushed or uterine horns were opened (day 24) for embryo recovery. In Experiment 1, 58 gilts (88 %) were cycling ones with mean number of ovulation of 10.6 ± 3.1. In first estrous gilts (n = 8) it was 11.1± 2.5. No differences were observed both between left and right ovaries, and first estrous and cycling gilts. The number of corpora lutea were correlated with ovarian weight (r = 0.35, p<0.05). Oviduct length (24.4 ± 1.4 and 24.3 ± 0.5 cm) and weight (4.3 ± 0.4 and 3.2 ± 0.2 g), and uterine horn length (143 ± 13 and 143 ± 5 cm) were not different in first estrous and cycling gilts. Uterine weight was higher in first estrous compared to cycling gilts (358 ± 32 vs. 250 ± 12 g, p < 0.05). In pregnant Mangalica (M) and Landrace (L) gilts no breed*day of pregnancy interaction regarding the number of ovulation was found. Mean number of corpora lutea was 17.2 ± 1.2 (M) and 18.6 ± 1.3 (L). Length of uterine horns was reduced in pregnant M compared to L gilts (124 ± 5 vs. 188 ± 6 cm, p < 0.01). Uterus grows in length continuously between day 1 and 24 of pregnancy in Landrace but not in Mangalica sows (p < 0.01). Uterine weight as well increased earlier (day 1 to day 12) in Landrace in comparison to Mangalica (day 12 to day 24). Results of this study support the concept that in Mangalica besides diminished ovarian and oocyte development, uterine conditions, especially growth restricted uterine development, may influence the initial process of early pregnancy and can be another reason of lower fecundity.

POSTWEANING CHANGES IN THE REPRODUCTIVE TRACT OF THE SOW

1983 ◽  
Vol 63 (3) ◽  
pp. 571-577 ◽  
Author(s):  
G. W. DYCK
Keyword(s):  
Key Words ◽  
Reproductive Tract ◽  
Ovarian Follicle ◽  
Follicular Development ◽  
Corpora Lutea ◽  
Uterine Weight ◽  
Follicle Growth ◽  
Ovarian Weight ◽  
Uterine Growth ◽  
Growth Changes

The postweaning growth changes in the reproductive tract of the sow were determined in 98 Yorkshire sows slaughtered at 2-day intervals from weaning (day 0) to day 16 postweaning. At the time of weaning, after a 42-day lactation, the reproductive tract resembled that of a prepubertal gilt or anestrous sow. From weaning to day 6 (estrus), the only observed change in the ovaries was an increase in size of the vesicular follicles. After ovulation there was a decrease (P < 0.01) in ovarian weight (day 6, 8.2 ± 0.3 g; day 8, 6.0 ± 0.5 g). Subsequently ovarian weight increased with development of the corpora lutea (day 16, 13.2 ± 0.9 g). Growth of the oviducts and cervix followed the pattern of ovarian follicle growth with an increase (P < 0.01) in weight and length until day 6 postweaning (7.5 ± 0.6 g, 51.7 ± 2.2 cm, and 108 ± 7 g, 15.9 ± 0.9 cm, respectively). After ovulation, the weight and length of the oviducts and the cervix declined to those observed on day 2 postweaning. Uterine weight increased (P < 0.01) from weaning to day 6 postweaning (137 ± 24 g to 371 ± 24 g). A small increase (P < 0.01) in length was observed by day 4 postweaning (125 ± 8 cm to 151 ± 5 cm). After ovulation, uterine weight decreased to 278 ± 15 g by day 8 postweaning. Subsequently both weight and length increased to day 16 postweaning (455 ± 31 g, 264 ± 11 cm). Thus, while growth of the oviducts, uterus and cervix were associated with preovulatory follicular development only uterine growth was postiviely associated with development of the corpora lutea. Key words: Sow, postweaning, reproductive tract growth

scholarly journals Carbohydrate binding activities of Bradyrhizobium japonicum. II. Isolation and characterization of a galactose-specific lectin.

1990 ◽  
Vol 111 (4) ◽  
pp. 1639-1643 ◽  
Author(s):  
S C Ho ◽  
M Schindler ◽  
J L Wang
Keyword(s):  
Isoelectric Focusing ◽  
Bradyrhizobium Japonicum ◽  
Affinity Column ◽  
Carbohydrate Binding ◽  
Binding Affinities ◽  
Isolation And Characterization ◽  
Relative Binding ◽  
Mannose Derivatives ◽  
Derivatives Of

Extracts of Bradyrhizobium japonicum were fractionated on Sepharose columns covalently derivatized with lactose. Elution of the material that was specifically bound to the affinity column with lactose yielded a protein of Mr approximately 38,000. Isoelectric focusing of this sample yielded two spots with pI values of 6.4 and 6.8. This protein specifically bound to galactose-containing glycoconjugates, but did not bind either to glucose or mannose. Derivatives of galactose at the C-2 position showed much weaker binding; there was an 18-fold difference in the relative binding affinities of galactose versus N-acetyl-D-galactosamine. These results indicate that we have purified a newly identified carbohydrate-binding protein from Bradyrhizobium japonicum, that can exquisitely distinguish galactose from its derivatives at the C-2 position.

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