THE EFFECTS OF CHOLINE AND SINAPINE BISULFATE IN A LAYING RATION ON THE INCIDENCE OF FISHY ODOR IN EGGS FROM BROWN-SHELLED EGG LAYERS

1979 ◽  
Vol 59 (3) ◽  
pp. 545-549 ◽  
Author(s):  
Y. K. GOH ◽  
M. M. MUELLER ◽  
D. R. CLANDININ ◽  
A. R. ROBBLEE
Keyword(s):  
Choline Chloride ◽  
Sinapic Acid ◽  
Rapeseed Meal ◽  
Fishy Odor ◽  
Comparable Level ◽  
Free Choline

Two experiments were conducted in which the effects of including in the ration free choline, supplied as choline chloride or as hydrolyzed sinapine (ester of sinapic acid and choline), and bound choline, supplied as sinapine from rapeseed meal, on the fishy odor and the trimethylamine (TMA) content of eggs laid by brown-shelled egg layers were assessed. The results indicated that at the levels fed, free choline does not cause the production of fishy eggs; however, 57% of the eggs produced by the birds fed a comparable level of bound choline in the form of sinapine had a fishy odor and contained appreciable amounts of TMA.

Influence of rapeseed meal treatments on its total phenolic content and composition in sinapine, sinapic acid and canolol

2015 ◽  
Vol 76 ◽  
pp. 1061-1070 ◽  
Author(s):  
Erika Zago ◽  
Jérôme Lecomte ◽  
Nathalie Barouh ◽  
Chahinez Aouf ◽  
Patrick Carré ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Sinapic Acid ◽  
Rapeseed Meal ◽  
Total Phenolic

THE EFFECT OF LEVEL OF SINAPINE IN A LAYING RATION ON THE INCIDENCE OF FISHY ODOR IN EGGS FROM BROWN-SHELLED EGG LAYERS

1979 ◽  
Vol 59 (2) ◽  
pp. 313-316 ◽  
Author(s):  
Y. K. GOH ◽  
D. R. CLANDININ ◽  
A. R. ROBBLEE ◽  
K. DARLINGTON
Keyword(s):  
Rhode Island ◽  
Rapeseed Meal ◽  
Fishy Odor

Triplicate groups of four Rhode Island Red layers which had been previously found to lay fishy eggs when fed a ration containing 10% of rapeseed meal (RSM) were fed a laying ration, devoid of RSM, to which 0, 0.5, 1.0, 2.0 or 4.0 g of sinapine (as bisulfate) per kilogram of ration was added. Eggs produced were analyzed for trimethylamine and scored organoleptically for fishyness. Inclusion of more than 1 g of sinapine per kilogram of laying ration caused the birds to lay eggs with a fishy odor. The findings suggest that when RSM is included in the ration of such birds, the level of usage should be such that the ration will contain not more than 0.1% sinapine.

scholarly journals Continuous adsorption in food industry: The recovery of sinapic acid from rapeseed meal extract

2021 ◽  
Vol 254 ◽  
pp. 117403
Author(s):  
Mónica Moreno-González ◽  
Daphne Keulen ◽  
Joaquín Gomis-Fons ◽  
Gustavo Lopez Gomez ◽  
Bernt Nilsson ◽  
...  
Keyword(s):  
Food Industry ◽  
Sinapic Acid ◽  
Rapeseed Meal ◽  
Continuous Adsorption

INFLUENCE OF GLUCOSINOLATES AND FREE OXAZOLIDINETHIONE IN A LAYING DIET CONTAINING A CONSTANT AMOUNT OF SINAPINE ON THE TRIMETHYLAMINE CONTENT AND FISHY ODOR OF EGGS FROM BROWN-SHELLED EGG LAYERS

1983 ◽  
Vol 63 (3) ◽  
pp. 671-676 ◽  
Author(s):  
Y. K. GOH ◽  
A. R. ROBBLEE ◽  
D. R. CLANDININ
Keyword(s):  
Key Words ◽  
Rhode Island ◽  
Aqueous Extract ◽  
Soybean Meal ◽  
Control Diet ◽  
Rapeseed Meal ◽  
The Other ◽  
Aqueous Extracts ◽  
Constant Amount ◽  
Fishy Odor

An experiment was conducted to study the effect that varying the amount of glucosinolates and free oxazolidinethione (OZT) in a laying diet containing a constant amount of sinapine had on the trimethylamine (TMA) content of eggs and the incidence of fishy eggs from brown-shelled egg layers. Duplicate groups of 10 Rhode Island Red laying hens which had been found to lay fishy eggs when fed a diet containing 10% rapeseed meal (RSM) were fed each of the four experimental diets. The diets consisted of a wheat-soybean-meal-type control diet and three diets to which aqueous extracts of RSM produced from high, medium and low glucosinolate cultivars (Oro, Span and Tower) were added. The extracts were included in the diet at levels of 3.42, 4.4 and 5.0%, respectively, to provide a sinapine content of 0.17%, a level equivalent to that which would result from the inclusion of 10% RSM in the diet. The results showed that the TMA content of eggs produced by the layers fed the diet containing Tower aqueous extract was significantly higher than that of eggs produced by birds fed the diets containing Oro or Span aqueous extracts (P < 0.05). Since the level of glucosinolates in the diets containing Oro and Span aqueous extracts were about seven times that in the diet containing the Tower aqueous extract, it may be concluded that intact glucosinolates do not affect the amount of TMA in eggs from brown-shelled egg layers fed a diet containing a constant amount of sinapine. On the other hand, free OZT in the diet increased the TMA content of the eggs and the incidence of fishy eggs produced by brown-shelled egg layers. Key words: Rapeseed meal, glucosinolate, oxazolidinethione, trimethylamine, fishy eggs, brown-shelled egg layers

Kinetics of the Na+-H+ antiporter as assessed by the change in intracellular pH in MDCK cells

1986 ◽  
Vol 251 (4) ◽  
pp. C558-C562 ◽  
Author(s):  
A. M. Selvaggio ◽  
J. H. Schwartz ◽  
H. H. Bengele ◽  
E. A. Alexander
Keyword(s):  
Intracellular Ph ◽  
Mdck Cells ◽  
Choline Chloride ◽  
Maximum Velocity ◽  
Ph Sensitive ◽  
Proton Efflux ◽  
Saturation Kinetics ◽  
Free Choline ◽  
Kinetics Of ◽  
Phi Regulation

The Na+-H+ antiporter regulates both H+ secretion and cell pH in renal epithelia. The present study was designed to further define the Na+ dependency and kinetics of proton efflux in MDCK cells. Intracellular pH (pHi) was determined with a pH-sensitive fluorescent probe, 2,7-bis-carboxyethyl-5,6-carboxyfluorescein (BCECF). Data were obtained from confluent monolayers grown on plastic cover slips and studied in media free of added CO2 and HCO-3, pH = 7.2 (pHo). Monolayers in NaCl maintain a pHi of 7.48 +/- 0.03 (n = 13). When cells were acidified with NH4Cl, pHi decreased to 6.73 +/- 0.07 (n = 10) and remained stable in Na+-free choline chloride. When monolayers were subsequently exposed to NaCl, pHi increased 0.28 +/- 0.04 pH units/min. Amiloride (2.5 mM) inhibited this Na+-dependent rise in pHi by more than 75%. The rate of pHi recovery after exposure to Na+ exhibited saturation kinetics; the apparent Km(Na) was 30.4 +/- 4.2 mM and maximum velocity was 107.1 +/- 17.1 delta [H+]i nmol X l-1 X min-1. We conclude that pHi regulation in the MDCK cell is in part mediated by a Na+-H+ antiporter, and the kinetics of this process can be reliably assessed by the pH-sensitive fluorometric probe, BCECF.

scholarly journals Na+ modulates carrier-mediated Fe2+ transport through the erythroid cell membrane

1991 ◽  
Vol 275 (3) ◽  
pp. 635-638 ◽  
Author(s):  
A Egyed
Keyword(s):  
Cell Membrane ◽  
Choline Chloride ◽  
Maximum Velocity ◽  
Erythroid Cell ◽  
Exchange System ◽  
Ph Optimum ◽  
Low Concentrations ◽  
Free Choline ◽  
Uptake Process ◽  
Rabbit Reticulocytes

The effects of univalent cations on Fe2+ uptake by rabbit reticulocytes have been studied. The rate of Fe2+ uptake was almost identical when measured in Na(+)-free choline chloride or KCl medium. Na+ but not Li+ inhibited Fe2+ uptake even at relatively low concentrations (2.5-20 mM) in these media. In contrast to this effect of extracellular Na+, the rate of Fe2+ uptake was facilitated by an increase in the intracellular Na2+ concentration in the range 5-40 mM, suggesting that intracellular Na+ is required for the uptake process. This effect also appears to be specific for Na+. Amiloride inhibited Fe2+ uptake in KCl (or in choline chloride), but had little if any effect in NaCl. Therefore a Na(+)- and amiloride-sensitive and a Na(+)- and amiloride-insensitive component can be distinguished. The two components differ in maximum velocity and pH optimum, but not in their apparent affinity for Fe2+. The ineffectiveness of selective inhibitors excludes the involvement of the Na+/H+ or Na+/Ca2+ exchange mechanisms. To account for the results presented in this work, a Na+/Fe2+ exchange system in the erythroid cell membrane is proposed.

scholarly journals Valorization Potentials of Rapeseed Meal in a Biorefinery Perspective: Focus on Nutritional and Bioactive Components

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6787
Author(s):  
Gabriella Di Lena ◽  
Jose Sanchez del Pulgar ◽  
Massimo Lucarini ◽  
Alessandra Durazzo ◽  
Petra Ondrejíčková ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Acids ◽  
Animal Feed ◽  
Sinapic Acid ◽  
Chemical Characterization ◽  
Rapeseed Meal ◽  
Emission Spectrometry ◽  
Total Phenolic ◽  
Array Detector

Rapeseed meal (RSM), a by-product of oilseed extraction connected to the agri-food and biofuel sectors, is currently used as animal feed and for other low-value purposes. With a biorefinery approach, RSM could be valorized as a source of bio-based molecules for high-value applications. This study provides a chemical characterization of RSM in the perspective of its valorization. A qualitative study of main functional groups by fourier transform infrared (FTIR) spectroscopy was integrated with a chemical characterization of macronutrients, minerals by inductively coupled plasma optical emission spectrometry (ICP-OES), phenolic acids and lipid components by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), HPLC-diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry/flame ionization detector (GC-MS/FID). The study, conducted on different lots of RSM collected over a one-year period from an oil pressing factory serving a biofuel biorefinery, highlighted a constant quality over time of RSM, characterized by high protein (31–34%), fiber (33–40%) and mineral (5.5–6.8%) contents. Polyphenol extracts showed a significant antioxidant activity and a prevalence of sinapic acid, accounting for more than 85% of total phenolic acids (395–437 mg kg−1 RSM). Results highlight the potentialities of RSM for further valorization strategies that may lead to the creation of new cross-sector interconnections and bio-based value chains with improvement of the economics and sustainability of the bioeconomy sectors involved.

A novel integrated downstream processing approach to recover sinapic acid, phytic acid and proteins from rapeseed meal

2015 ◽  
Vol 90 (11) ◽  
pp. 1999-2006 ◽  
Author(s):  
Alexander Thiel ◽  
Kai Muffler ◽  
Nils Tippkötter ◽  
Kirstin Suck ◽  
Ulrich Sohling ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Sinapic Acid ◽  
Rapeseed Meal ◽  
Downstream Processing ◽  
Processing Approach

Recovery of sinapic acid from canola/rapeseed meal extracts by adsorption

2020 ◽  
Vol 120 ◽  
pp. 69-79 ◽  
Author(s):  
Mónica Moreno-González ◽  
Vasupradha Girish ◽  
Daphne Keulen ◽  
Hilde Wijngaard ◽  
Xavier Lauteslager ◽  
...  
Keyword(s):  
Sinapic Acid ◽  
Rapeseed Meal
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