COPPER STORAGE IN THE LIVERS OF CATTLE SUPPLEMENTED WITH INJECTED COPPER AND WITH COPPER SULFATE AND CHELATED COPPER

1978 ◽  
Vol 58 (3) ◽  
pp. 525-529 ◽  
Author(s):  
J. E. MILTIMORE ◽  
C. M. KALNIN ◽  
J. B. CLAPP
Keyword(s):  
Liver Biopsy ◽  
Copper Sulfate ◽  
Sodium Molybdate ◽  
Dry Weight ◽  
Weight Basis ◽  
Test Period ◽  
Average Increase ◽  
Copper Supplementation ◽  
Ad Libitum ◽  
Copper Storage

Cattle fed ad libitum a mixture of salt containing 0.5% copper as copper sulfate had an average increase in Cu concentration from 111 to 328 ppm on a dry weight basis in liver biopsy samples over a 333-day test period. The alfalfa hay ration contained an average of 8.2 ppm Cu and a relatively high copper to molybdenum ratio of 3.9:1. Copper supplementation from a variety of sources was given orally or by injection in an 87-day test. Chelated Cu was compared with Cu in copper sulfate, both mixed with salt, and with Cu injected as Cu calcium edetate. The ration of mixed alfalfa and grass contained 6.9 ppm Cu with sufficient sodium molybdate added to produce a Cu/Mo ratio of unity against which the various Cu sources were tested. There was no significant (P < 0.05) effect of Cu source on Cu storage in the liver. There were no trends (P < 0.10) for chelated Cu to result in higher levels of Cu in the liver than when Cu was supplied as copper sulfate even when chelated Cu was fed as fivefold the recommended levels.

Copper status of sheep grazing pastures fertilized with sulfur and molybdenum

1981 ◽  
Vol 32 (3) ◽  
pp. 479 ◽  
Author(s):  
JP Langlands ◽  
JE Bowles ◽  
GE Donald ◽  
AJ Smith ◽  
DR Paull
Keyword(s):  
Copper Sulfate ◽  
Copper Concentration ◽  
Sodium Molybdate ◽  
Sheep Grazing ◽  
Biopsy Technique ◽  
Black Sheep ◽  
Hepatic Copper ◽  
Copper Status ◽  
Copper Storage ◽  
Molybdenum Concentration

Varying quantities of sodium molybdate, superphosphate and copper sulfate were applied to 30 plots, and changes in hepatic copper concentration in sheep grazing these plots were measured by using a biopsy technique. A relationship between changes in hepatic copper storage and the copper and sulfur x molybdenum concentrations in the green forage on offer was calculated. This relationship was used to calculate the quantity of copper in green forage on offer necessary to maintain hepatic copper concentration constant when the forage varied in sulfur and molybdenum concentration. In a second experiment sodium molybdate was applied to pasture grazed by sheep at rates varying from 0 to 2000 g molybdenum per ha. Black sheep grazing pasture dressed with 2000 g molybdenum per ha, grew white wool for four months following molybdenum application; this corresponded to the period when molybdenum concentration in herbage on offer was greatest. There were also changes in blood, wool, kidney and liver composition following molybdenum application but these were small when molybdenum was applied at the usual commercial rate. The results of both experiments suggest that the application of molybdenum to pasture at commercial rates is unlikely to induce copper deficiencies in grazing sheep.

SUFFICIENCY OF CU AND ZN IN BARLEY, FORAGE, AND CORN SILAGE RATIONS AS MEASURED BY RESPONSE TO SUPPLEMENTS BY BEEF CATTLE

1973 ◽  
Vol 53 (3) ◽  
pp. 497-502 ◽  
Author(s):  
W. L. PRINGLE ◽  
W. K. DAWLEY ◽  
J. E. MILTIMORE
Keyword(s):  
Average Daily Gain ◽  
Dry Weight ◽  
Weight Basis ◽  
Corn Silage ◽  
Feed Consumption ◽  
Beef Steers ◽  
Peace River ◽  
Copper Supplementation ◽  
Daily Gain ◽  
Cu And Zn

Beef heifers of 232 and 205 kg average beginning weight, fed individually in a barn in two separate trials, were assigned at random to the following treatments: (A) basic barley–hay ration containing 3–1 ppm Cu and 25–32 ppm Zn; (B) basic ration supplemented to 11–12 ppm Cu; (C) basic ration supplemented to 63–66 ppm Zn. The Cu-supplemented ration resulted in an increased amount of Cu in the liver (P < 0.01). The Zn-supplemented ration resulted in a decreased amount of Cu in the liver in trial 1 (P < 0.05) but did not affect the level of Zn either in the liver or in the hair. No difference was found in average daily gain, feed consumption, efficiency of gain, or dressing percent of the heifers. It was shown that under a full-feed regime for 134 and 151 days, young animals (heifers) with Cu levels in the liver of 100 ppm or more on a dry weight basis can maintain normal metabolic balance when their ration is less than 4 ppm Cu and 33 ppm Zn. Barley and forage grown on Grey Wooded soil of the Peace River area, even though analytically low in Cu and Zn, are sufficient for heifers on full feed. In a third trial, beef steers were fed for 112 days on corn silage fortified with urea. Copper glycinate injections showed no effect on gain, although final concentrations of Cu in the liver were increased from 28 to 133 ppm on a dry weight basis. The corn silage contained 7 ppm Cu and had a Cu/Mo ratio of 6:6. Copper supplementation of corn silage rations is not warranted with this level of copper and a favorable Cu/Mo ratio.

ERRORS ASSOCIATED WITH HERBAGE DISSECTION ANALYSES 2. Problems of species identification

1976 ◽  
pp. 213-225
Author(s):  
I.M. Ritchie ◽  
C.C. Boswell ◽  
A.M. Badland
Keyword(s):  
New Zealand ◽  
Dry Weight ◽  
Analytical Tool ◽  
Species Level ◽  
Weight Basis ◽  
Plant Identification ◽  
Leaf Fragment ◽  
Research Division ◽  
Or Groups ◽  
Botanical Analysis

HERBACE DISSECTION is the process in which samples of herbage cut from trials are separated by hand into component species. Heavy reliance is placed on herbage dissection as an analytical tool ,in New Zealand, and in the four botanical analysis laboratories in the Research Division of the Ministry of Agriculture and Fisheries about 20 000 samples are analysed each year. In the laboratory a representative subsample is taken by a rigorous quartering procedure until approximately 400 pieces of herbage remain. Each leaf fragment is then identified to species level or groups of these as appropriate. The fractions are then dried and the composition calculated on a percentage dry weight basis. The accuracy of the analyses of these laboratories has been monitored by a system of interchanging herbage dissection samples between them. From this, the need to separate subsampling errors from problems of plant identification was, appreciated and some of this work is described here.

Polycyclic Aromatic Hydrocarbons (PAHs) in Sediments of the Brisbane River (Australia) – Preliminary Results

1989 ◽  
Vol 21 (2) ◽  
pp. 161-165 ◽  
Author(s):  
S. I. Kayal ◽  
D. W. Connell
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
River Estuary ◽  
Dry Weight ◽  
Weight Basis ◽  
Sediment Samples ◽  
Preliminary Results ◽  
Treated Sewage ◽  
Polycyclic Aromatic ◽  
Petroleum Refineries

Results of the analysis of twenty-three composite sediment samples revealed that PAHs are widely distributed in the Brisbane River estuary. Mean concentrations for individual compounds, on a dry weight basis, ranged from 0.03 µg/g for dibenz [ah] anthracene to 2.34 µg/g for fluoranthene. Observed PAH assemblages were rich in compounds having pyrolytic origins. However, the presence of petroleum derived compounds was indicative of the importance of petroleum as a PAH source in the estuary. Petroleum refineries, a coal loading terminal and a major treated sewage outfall located at the mouth were not indicated as major contributing sources of PAH pollution in the estuary.

Mineral nutrition, dinitrogen fixation, and growth of Alnuscrispa and Alnusglutinosa

1985 ◽  
Vol 15 (5) ◽  
pp. 855-861 ◽  
Author(s):  
G. Prégent ◽  
C. Camiré
Keyword(s):  
Mineral Nutrition ◽  
Dry Weight ◽  
Optimal Growth ◽  
Maximum Growth ◽  
Dinitrogen Fixation ◽  
Weight Basis ◽  
Critical Levels ◽  
N Status

Invitro cultures of Alnuscrispa (Ait.) Pursh and Alnusglutinosa (L.) Gaertn. were used to estimate critical foliage levels of selected nutrients for optimal growth and dinitrogen (N2) fixation. For A. crispa to obtain 90% of maximum growth and N2 fixation, foliar levels of 0.12% P, 0.13% Mg, <0.31% K, and <0.04% Ca on a dry weight basis were needed. For A. glutinosa, the critical levels were 0.138% P, 0.10% Mg, 0.29% Ca, and ~0.20% K. From all the deficiencies observed, P had the more pronounced effects on N status of both species.

SOLUBLE SUGAR CHANGES OCCURRING DURING COLD HARDENING OF SPRING WHEAT, FALL RYE AND ALFALFA

1983 ◽  
Vol 63 (2) ◽  
pp. 415-420 ◽  
Author(s):  
D. G. GREEN
Keyword(s):  
Spring Wheat ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Weight Basis ◽  
Cold Hardening ◽  
Spring Type

Alfa, a relatively nonhardy alfalfa cultivar continued to accumulate, on a dry weight basis, fructose, α- and β-D-glucose, sucrose and maltose during the latter stages of cold hardening. Rambler, a hardier alfalfa cultivar conversely showed a decrease for these soluble sugars with hardening. Frontier rye, a very hardy winter habit cereal showed decreases in these soluble sugars plus melibiose during the same hardening period. These results support the hypothesis that hardy cereals and alfalfa undergo a decrease in soluble sugars with hardening, while less hardy cereals and alfalfa continue to increase in content of soluble sugars. Manitou wheat appeared not to fit this hypothesis and showed the decreased soluble sugars usually associated with hardy cultivars. Although Manitou is a spring type wheat, one of its parents, Thatcher, does contain gene(s) for the winter habit.Key words: Sugar, cold hardening, wheat, rye, alfalfa

scholarly journals THE MOLECULAR WEIGHT OF RHODOPSIN AND THE NATURE OF THE RHODOPSIN-DIGITONIN COMPLEX

1954 ◽  
Vol 37 (3) ◽  
pp. 381-399 ◽  
Author(s):  
Ruth Hubbard
Keyword(s):  
Molecular Weight ◽  
Single Molecule ◽  
Extinction Coefficient ◽  
Dry Weight ◽  
Weight Basis ◽  
Sedimentation Behavior ◽  
A Value ◽  
Wet Weight ◽  
Single Boundary ◽  
Stoichiometric Complex

The sedimentation behavior of aqueous solutions of digitonin and of cattle rhodopsin in digitonin has been examined in the ultracentrifuge. In confirmation of earlier work, digitonin was found to sediment as a micelle (D-1) with an s20 of about 6.35 Svedberg units, and containing at least 60 molecules. The rhodopsin solutions sediment as a stoichiometric complex of rhodopsin with digitonin (RD-1) with an s20 of about 9.77 Svedberg units. The s20 of the RD-1 micelle is constant between pH 6.3 and 9.6, and in the presence of excess digitonin. RD-1 travels as a single boundary also in the electrophoresis apparatus at pH 8.5, and on filter paper at pH 8.0. The molecular weight of the RD-1 micelle lies between 260,000 and 290,000. Of this, only about 40,000 gm. are due to rhodopsin; the rest is digitonin (180 to 200 moles). Comparison of the relative concentrations of RD-1 and retinene in solutions of rhodopsin-digitonin shows that RD-1 contains only one retinene equivalent. It can therefore contain only one molecule of rhodopsin with a molecular weight of about 40,000. Cattle rhodopsin therefore contains only one chromophore consisting of a single molecule of retinene. It is likely that frog rhodopsin has a similar molecular weight and also contains only one chromophore per molecule. The molar extinction coefficient of rhodopsin is therefore identical with the extinction coefficient per mole of retinene (40,600 cm.2 per mole) and the E(1 per cent, 1 cm., 500 mµ) has a value of about 10. Rhodopsin constitutes about 14 per cent of the dry weight, and 3.7 per cent of the wet weight of cattle outer limbs. This corresponds to about 4.2 x 106 molecules of rhodopsin per outer limb. The rhodopsin content of frog outer limbs is considerably higher: about 35 per cent of the dry weight, and 10 per cent of the wet weight, corresponding to about 2.1 x 109 molecules per outer limb. Thus the frog outer limb contains about five hundred times as much rhodopsin as the cattle outer limb. But the relative volumes of these structures are such that the ratio of concentrations is only about 2.5 to 1 on a weight basis. Rhodopsin accounts for at least one-fifth of the total protein of the cattle outer limb; for the frog, this value must be higher. The extinction (K500) along its axis is about 0.037 cm.2 for the cattle outer limb, and about 0.50 cm.2 for the frog outer limb.

Influence of Temperature and Water Activity on Aflatoxin Production by Aspergillus flavus in Cowpea (Vigna unguiculata) Seeds and Meal

1985 ◽  
Vol 48 (12) ◽  
pp. 1040-1043 ◽  
Author(s):  
P. E. KOEHLER ◽  
L. R. BEUCHAT ◽  
M. S. CHHINNAN
Keyword(s):  
Heat Treatment ◽  
Water Activity ◽  
Aspergillus Flavus ◽  
Vigna Unguiculata ◽  
Dry Weight ◽  
Aflatoxin Production ◽  
Weight Basis ◽  
Influence Of Temperature

Experiments were done to determine the influence of temperature (21, 30 and 37°C) and aw (0.76 to 0.98) on aflatoxin production by Aspergillus flavus on cowpea (Vigna unguiculata) seeds, meal and meal supplemented with onion. Larger quantities of aflatoxin were produced at 21 and 30°C than at 37°C. The highest amount of aflatoxin (2777 μg/20 g, dry weight basis) was observed in meal containing onion at aw 0.98 after 20 d of incubation at 21°C. A level of 870 |μg/20 g was detected in seeds at aw 0.95 after 14 d of incubation at 30°C. Meal at aw 0.96 supported production of 551 μg of aflatoxin per 20 g after 20 d at 30° C. Temperature had little influence on the optimal aw for aflatoxin production in cowpea meal. However, an increase in temperature resulted in a decreased optimal aw for aflatoxin production on whole cowpeas. When known quantities of aflatoxin were added to cowpea meal which was subsequently steamed for 5 min, only 29% was extractable using a variety of procedures, indicating that the toxin may be bound in some manner to cowpea constituents as a result of heat treatment.

THE EFFECT OF INGESTED CHLORTETRACYCLINE ON SOME HYDROLASES AND ORGANS ASSOCIATED WITH THE DIGESTIVE PROCESS IN GROWING PIGS: II. PROTEASE, AMYLASE, AND GELLULASE ACTIVITY OF THE CONTENTS OF THE SMALL INTESTINE AND OF THE CECUM; WEIGHTS OF SMALL INTESTINE AND MUCOSA

1957 ◽  
Vol 35 (3) ◽  
pp. 195-202 ◽  
Author(s):  
M. A. A. Vonk ◽  
L. W. McElroy ◽  
R. T. Berg
Keyword(s):  
Small Intestine ◽  
Dry Matter ◽  
Cellulase Activity ◽  
Dry Weight ◽  
Growing Pigs ◽  
Weanling Pigs ◽  
Intestinal Contents ◽  
Wet Weight ◽  
The Mean ◽  
Ad Libitum

Four treatments, involving differences in method of feeding and interval between last feed and slaughter, were employed in a study of the effect of dietary chlortetracycline on protease, amylase, and cellulase activity in the intestinal and cecal contents of 16 pairs of weanling pigs. Most consistent results were obtained with six pairs which were limited pair-fed except for the final feeding during which feed was available ad libitum for a 4-hour period ending 18 hours before slaughter. The mean total activities of all three hydrolases in the contents of the small intestines and of the ceca of the antibiotic-fed animals of these six pairs were significantly greater than in those of the control animals. Expressed as activity per gram dry matter of intestinal contents, significantly higher values for protease and amylase, but not for cellulase, were observed in the pigs that had received chlortetracycline. When the combined results obtained from all 16 pairs of the experimental animals were analyzed, the results showed that on a basis of activity per gram dry matter of intestinal contents, ingested chlortetracycline was associated with significant increases in amylase and cellulase but not in protease activity. Protease, amylase, and cellulase activities per gram dry matter of cecal contents were higher for pigs fed the antibiotic than for their controls. The mean wet weight of the empty small intestine and the mean dry weight of the mucosa scraped from the anterior 3-meter section of the small intestine were lower for the chlortetracycline-fed animals, but the differences were not statistically significant.

Low K+ in Paphiopedilum leeanum leaf epidermis: implications for stomatal functioning

1977 ◽  
Vol 55 (4) ◽  
pp. 489-495 ◽  
Author(s):  
Sherman D. Nelson ◽  
James M. Mayo
Keyword(s):  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Weight Basis ◽  
Leaf Epidermis ◽  
Photometric Analysis ◽  
Abaxial Epidermis ◽  
Absorption Spectrophotometry ◽  
Stomatal Functioning ◽  
Low K ◽  
Stomatal Mechanism

Abaxial epidermal strips from leaves of Paphiopedilum leeanum were analyzed via sodium cobaltinitrite staining and atomic absorption spectrophotometry for the presence and location of potassium. On a dry weight basis K content of the abaxial epidermis was found to be 103 times less than has been reported in other species, and unlike other species no localization of K+ in guard cells of open stomata could be detected via the sodium cobaltinitrite stain for potassium.Flame photometric analysis of the mesophyll indicated that it contained normal amounts of K+ (about 1.87% on a dry weight basis). Analysis showed that the K+ content of the abaxial epidermis (0.032%) was considerably less than that of the mesophyll, a situation unlike previous reports for other species in which the epidermal concentration was found to be greater than the mesophyll. A process for exclusion of K+ from the abaxial epidermis is suggested, as is the lack of involvement of K+ as the major osmoticum in the stomatal mechanism of this species.

Sign in / Sign up

Export Citation Format

Share Document