PLASMA AMINO ACIDS IN COWS FED HIGH-FIBER UREA-SUPPLEMENTED DIETS

1975 ◽  
Vol 55 (4) ◽  
pp. 715-720 ◽  
Author(s):  
R. BOUCHARD ◽  
H. R. CONRAD
Keyword(s):  
Amino Acids ◽  
Milk Production ◽  
Dry Matter ◽  
Essential Amino Acids ◽  
Feed Consumption ◽  
Plasma Amino Acids ◽  
High Fiber ◽  
Fiber Diet ◽  
Daily Rate ◽  
Starch Diet

A diet containing, on a dry matter basis, 41.8% acid detergent fiber and 17.9% crude protein, of which 55% was obtained from urea, was compared for lactating dairy cows with a diet in which nitrogen was supplied from vegetable protein, and most of the total digestible nutrients were from starch. Dry matter intake was 17.6 and 14.9 kg per day (P < 0.05), 4% fat-corrected milk production was 23.2 and 20.8 kg per day (P < 0.05), and total levels of essential amino acids were 159.78 and 176.14 μg/ml (P < 0.05), respectively, for the cows receiving the protein–starch diet, compared with those receiving the urea–fiber diet. Except for serine, proline, cystine, asparagine and threonine, the plasma amino acids were lower in cows fed the protein–starch diet than in cows fed the fiber–urea diet. The plasma content of all amino acids except aspartic acid, glutamic acid and cystine increased consistently during the 1st mo of lactation. This elevation was coincidental with an increasing daily rate of feed consumption and a more rapidly increasing milk production.

EFFECTS OF VARIOUS DIETARY FATS ON THE POSTPRANDIAL CONCENTRATIONS OF BLOOD PLASMA-FREE AMINO ACIDS IN YOUNG DAIRY CALVES

1975 ◽  
Vol 55 (2) ◽  
pp. 257-268 ◽  
Author(s):  
D. D. RONY ◽  
G. J. BRISSON ◽  
M. DESMARAIS
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Dry Matter ◽  
Corn Oil ◽  
Essential Amino Acids ◽  
Dietary Fats ◽  
Butter Oil ◽  
Animal Fats ◽  
Daily Rate

Six groups of Holstein male calves, 4–5 days of age, were fed milk replacers containing, on a dry matter basis, 24.8% of either butter oil, lard, lard and corn oil (2:1), hydrogenated corn oil, or corn oil. Whole milk was used as control and the experiment lasted 56 days. The calves were fed, twice each day, a milk replacer containing 13% solids which was offered at a maximum daily rate of 12% of body weight as measured weekly. On the 28th and 56th day of the experiment, blood samples were drawn from the left jugular vein before the morning meal, and then 1, 2, 3, 4, 5, 7, 9, 11 and 13 h after feeding. Dry matter intake and growth rate were the lowest with calves receiving the corn oil diet. The calves fed corn oil also exhibited more frequent diarrhea. The vegetable fats, as compared to the animal fats, tended to induce a lower concentration of essential amino acids in blood plasma, but this trend was not statistically significant [Formula: see text] in all cases. The concentration of nonessential amino acids followed the same general trend as noted with essential amino acids. The concentration of total amino acids was the lowest for calves fed corn oil. Amino acid levels in blood varied with time after feeding, and exhibited postprandial patterns that differed from one amino acid to another or one group of amino acids to another. It would appear that type of dietary fat, under the conditions of this experiment, had an influence on amino acid metabolism in the young calf.

scholarly journals Immune Status and Growth of Broiler Fed Diet with Microparticle Protein Added with Natural Acidifier

2021 ◽  
Vol 44 (2) ◽  
pp. 198-204
Author(s):  
N. Suthama ◽  
B. Sukamto ◽  
I. Mangisah ◽  
L. Krismiyanto
Keyword(s):  
Amino Acids ◽  
Immune Status ◽  
Body Weight Gain ◽  
Immunoglobulin A ◽  
Essential Amino Acids ◽  
Feed Consumption ◽  
Intact Protein ◽  
Feed Conversion ◽  
Lime Juice ◽  
Randomized Design

The present study was conducted to evaluate the feeding effect of diet composed of microparticle protein added with lime juice as an acidifier on immune status and efficiency of protein utilization in relation to the growth of broiler chicken. Dietary treatments tested were P1 was diet with 20% intact protein and CaCO3 as a Ca source, P2 was diet with 20% microparticle protein and eggshell as a Ca source added with 1.2% lime juice, and P3 was diet with 20% microparticle protein and eggshell as a Ca source added with 2.4% lime juice. Variables measured were protein and amino acids digestibility, retentions of N and Ca, fecal Nτ–methyl histidine (Nτ–MH) excretion as a protein turnover index, lymphoid organs, heterophil and lymphocyte (H/L) ratio, immunoglobulin A (IgA) as a body resistance indicator, feed consumption, body weight gain (BWG), and feed conversion ratio (FCR). The experiment was arranged in a completely randomized design with 3 treatments and 8 replications. Data were subjected to analysis of variance and Duncan test at 5% probability. Protein and amino acid digestibility, N, and Ca retentions were significantly (p<0.05) higher in P2 and P3. However, the H/L ratio was significantly (p<0.05) lower in both treatments compared to P1. Bursa fabricius and lymph weights were not difference, but BWG in P2 significantly (p<0.05) increased. In conclusion, protein and some essential amino acids digestibility, N and Ca retentions, IgA, and BWG increase with the decrease in H/L ratio, fecal Nτ–MH, feed consumption, and FCR in broiler fed diet composed of microparticle protein source with additional 1.2 mL acidifier of lime juice.

scholarly journals Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

2008 ◽  
Vol 17 (4) ◽  
pp. 351 ◽  
Author(s):  
L. KRÍZOVÁ ◽  
J. TRINÁCTÝ ◽  
M. RICHTER
Keyword(s):  
Amino Acids ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Protein ◽  
Experimental Period ◽  
Dry Matter Intake ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Milk Protein Content ◽  
Ad Libitum

The aim of this study was to determine the influence of leucine supplement in the form of rumen-protected tablets on milk yield and composition and plasma amino acids in four high-yielding lactating Holstein cows. The experiment was carried out as a cross-over procedure and was divided into 4 periods of 14 d (10 d preliminary period and 4 d experimental period). Cows were fed ad libitum a diet based on maize silage, lucerne hay and a supplemental mixture. The diet, defficient in methionine, lysine, and leucine, was supplemented with methionine+lysine (Control) or methionine+lysine+leucine (Leu) in rumen protected form. The dry matter intake, milk yield and milk yield expressed in energy corrected milk did not differ significantly between the treatments. Milk protein content and yield did not show statistically significant variation. The contents and yield of casein, fat, lactose and urea were unaffected by the treatment. Blood metabolites did not vary between the treatments. The introduction of Leu resulted in higher plasma levels of proline (p

The effect of starvation and low nitrogen intakes on the concentration of free amino acids in the blood plasma and on the nitrogen metabolism in sheep

1970 ◽  
Vol 21 (5) ◽  
pp. 723 ◽  
Author(s):  
J Leibholz
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Matter ◽  
Control Diet ◽  
Amino Nitrogen ◽  
Essential Amino Acids ◽  
Isoleucine Leucine ◽  
Plasma Urea ◽  
Low Nitrogen

Crossbred wethers were given a control diet (8 g nitrogen, 730 g dry matter daily) or a low nitrogen diet (0.5 g nitrogen, 520 g dry matter daily) or starved, for a 12 or 20 day experimental period. The concentrations of free serine, glutamine, glycine, alanine, histidine, and arginine in the plasma of the starved sheep decreased significantly while the concentrations of lysine, 3-methylhistidine, and isoleucine increased significantly. The ratio of essential to non-essential amino acids increased from 0.35 to 0.56 in the starved sheep. In sheep on the low nitrogen diet, the ratio of essential to non-essential amino acids in the plasma decreased from 0.40 to 0.27, with significant increases in the concentrations of glutanlic acid, glutamine, glycine, isoleucine, leucine, and 3-methylhistidine. Starvation and the low nitrogen diet both resulted in a reduction of the plasma urea concentrations. Starvation and the low nitrogen diet resulted in a 20-50 % reduction in the flow of saliva and a 40-78% increase in the concentration of total nitrogen. This resulted in no significant change in the daily secretion of nitrogen in the saliva. The concentration of urea in the saliva was increased by 3-54%. The concentrations of individual free amino acids in saliva are reported. The nitrogen content of the rumen was reduced, and after 7 days of starvation or on the low nitrogen diet all rumen nitrogen could be attributed to ammonia and free �-amino nitrogen.

Effect of post-ruminal infusion of glutamine on plasma amino acids, milk yield and composition in lactating dairy cows

2001 ◽  
Vol 81 (2) ◽  
pp. 229-235 ◽  
Author(s):  
J. C. Plaizier ◽  
J. -P. Walton ◽  
B. W. McBride
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Composition ◽  
Plasma Amino Acid ◽  
Dry Matter Intake ◽  
Plasma Amino Acids

The objectives of this study were to examine the effect of supplying post-ruminal L-glutamine in mid-lactation Holstein dairy cows on plasma amino acid profile, dry matter intake, milk yield and milk composition. The experiment was designed as a 4 × 4 Latin square with four 2-wk periods. Cows were continuously infused post-ruminally with graded levels of L-glutamine (L-Gln) for 5 consecutive days during the second week of each period. During the last 24 h of the infusion, blood plasma was collected every 6 h for the determination of plasma amino acid concentrations. Plasma Gln concentration increased (P < 0.05) with increasing L-Gln daily infusions. Infusion of 0, 100, 200, and 300 g d–1 resulted in blood plasma Gln concentrations of 289.5, 299.2, 356.4 and 386.2 mmol L–1, respectively. The level of Gln infusion also resulted in a decrease in the blood plasma concentration of phenylalanine, but the concentrations of all other amino acids were not affected. The administration of Gln did not affect dry matter intake, milk yield and milk composition. Key words: Post-ruminal, L-glutamine, plasma amino acids, milk constituents

67. The Nutritive Value of Proteins for Milk Production. II. A Comparison of the Proteins of Blood Meal, Pea Meal, Decorticated Earth-Nut Cake, and a Mixture of Decorticated Earth-Nut Cake and Flaked Maize

1933 ◽  
Vol 5 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Samuel Morris ◽  
Norman C. Wright
Keyword(s):  
Amino Acids ◽  
Milk Production ◽  
Milk Yield ◽  
Blood Meal ◽  
Marked Reduction ◽  
Nutritive Value ◽  
Essential Amino Acids ◽  
The Other ◽  
Body Tissue ◽  
Food Protein

The results of the present experiments and of those reported in the previous paper(1) may be summarised as follows:1. When minimal quantities of protein are fed in the production rations of milking cows, a deficiency of either lysine or tryptophane will lead to a marked reduction in milk yield. There is, however, some evidence of the storage of reserve N, which can be utilised when the food protein is inadequate.2. The feeding of a lysine- or tryptophane-deficient ration causes a marked increase in urinary N, indicating a poor utilisation of food protein. On the other hand, the feeding of a ration containing adequate quantities of these essential amino acids reduces the urinary N, indicating efficient protein utilisation.3. The utilisation of body tissue in an attempt to maintain normal milk production on a deficient protein ration is shown by the high creatine excretion. The fact that the S: N ratio of the excess sulphur and nitrogen excreted during the deficient protein periods approximates that of body tissue (circa 1: 15·7) confirms this conclusion.

RESPONSE OF LACTATING COWS TO THE INTRAVENOUS INFUSION OF AMINO ACIDS

1972 ◽  
Vol 52 (2) ◽  
pp. 377-384 ◽  
Author(s):  
L. J. FISHER
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Milk Production ◽  
Milk Protein ◽  
Corn Silage ◽  
Protein Yield ◽  
Plasma Amino Acids ◽  
Lactating Cows ◽  
Limiting Amino Acid ◽  
High Level

High, low, and zero quantities of methionine, histidine, or lysine were infused intravenously into lactating cows fed a corn–corn silage–urea ration to assess whether any of these amino acids were limiting for milk production. Feed intake was significantly higher (P < 0.05) with the low level of methionine infusion than with either the high level or saline infusions. Milk yield was not affected (P > 0.05) by any treatments, but protein yield was significantly greater (P < 0.05) with the low than with the high or zero levels of infusion of methionine, and the infusion of either level of histidine significantly decreased (P < 0.05) milk protein yield compared with zero level of infusion. The changes in levels of plasma amino acids that occurred during these studies have been interpreted as implying that methionine was the first limiting and either valine, leucine, or isoleucine was the second limiting amino acid for milk production.

scholarly journals Effect of Sakura Block on Milk Production and Milk Quality of FH Cows in Late Lactation

2021 ◽  
Vol 16 (3) ◽  
pp. 266-272
Author(s):  
Jarmuji Jarmuji ◽  
D. Suherman ◽  
Yanuri Yanuri ◽  
R. Afriansyah ◽  
E. Sulistyowati
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Sample ◽  
Dry Matter ◽  
Milk Protein ◽  
Latin Square ◽  
Fat Content ◽  
Feed Consumption ◽  
Sample Collection

This study aims to evaluate milk production, fat content, and protein content of Friesian Holland (FH) dairy cow's milk by giving Sakura block at the final lactation of FH cow. The design used was the Latin Square Design (LSD), with treatments of P0 (0 Sakura blocks), P1 (300 gr Sakura blocks), P2 (600 gr Sakura blocks), and P3 (900 gr Sakura blocks). This study involved four dairy cows for four periods of which period consisted of 10 treatment days, where the last 3 days of each period were the milk sample collection. Milk production and feed consumption were measured and weighed daily. The results showed that the treatment had a significant effect (P <0.05) on the consumption of dry matter ratio and no significant effect (P> 0.05) on milk production, fat content, and milk protein.

RESPONSE OF DAIRY COWS TO FORMALDEHYDE-TREATED CANOLA MEAL DURING EARLY LACTATION

1983 ◽  
Vol 63 (4) ◽  
pp. 905-915 ◽  
Author(s):  
R. C. RAE ◽  
J. R. INGALLS ◽  
J. A. McKIRDY
Keyword(s):  
Amino Acids ◽  
Milk Production ◽  
Plasma Levels ◽  
Experimental Period ◽  
Essential Amino Acids ◽  
Milk Composition ◽  
Canola Meal ◽  
Plasma Urea ◽  
Increase Milk Production ◽  
Isocaloric Diets

Forty Holstein cows were assigned to one of four isocaloric diets 2 wk postpartum in a 2 × 2 factorial arrangement. Factor I was dietary crude protein (CP) level, either 17.4% or 14.6% CP. Factor II was formaldehyde (FA) treatment of canola meal, either treated or untreated. The experimental period lasted 12 wk. Treatment with FA had no effect on milk yield, milk composition, dry matter (DM) intake or on apparent digestibilities of DM, nitrogen (N) or acid detergent fiber (ADF). FA treatment reduced ammonia-N (NH3-N) levels in rumen liquor (P < 0.05), tended to increase (P < 0.1) plasma levels of total essential amino acids and had no effect on plasma urea. High dietary CP increased (P < 0.05) milk and lactose yields, had no effect on rumen liquor NH3-N levels and increased (P < 0.05) plasma levels or urea and total essential amino acids. Treatment with FA appears to have increased absorption of total essential amino acids from the gut but did not increase milk production. Possibly, amino acid supply to the mammary gland did not limit milk synthesis in this experiment. Alternatively, FA treatment may have failed to increase absorption of the amino acids whose availability did limit milk synthesis. FA treatment apparently destroyed 58% of canola meal tyrosine and 29% of the lysine and this may be partially responsible for the lack of a response in milk production. Key words: Holstein, formaldehyde, canola meal, milk production, protein level, amino acids

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