PLASMA LEVELS OF ESTRONE SULFATE, ESTRONE AND ESTRADIOL-17β IN THE COW AROUND PARTURITION

1975 ◽  
Vol 55 (4) ◽  
pp. 509-512 ◽  
Author(s):  
C. P. W. TSANG ◽  
A. J. HACKETT ◽  
E. M. TURNER Jr.

Estrone sulfate, estrone, and estradiol-17β have been assayed in blood plasma taken from the mammary vein of five pregnant cows before and after parturition. While there were considerable individual variations, the mean plasma levels of estrone sulfate (13.4 ng/ml), estrone (1 ng/ml) and estradiol-17β (1 ng/ml) remained fairly constant over the period of blood collection prior to parturition (−88 h to −2 h). The levels of all three estrogens dropped rapidly within 8 h postpartum. It is concluded that estrone sulfate, rather than estrone, is the predominant plasma estrogen in late pregnancy in the cow.

1974 ◽  
Vol 77 (2) ◽  
pp. 401-407 ◽  
Author(s):  
J. A. Mahoudeau ◽  
A. Delassalle ◽  
H. Bricaire

ABSTRACT Plasma levels of testosterone (T) and 5α-dihydrotestosterone (DHT) were determined by radioimmunoassay in 29 patients with benign prostatic hypertrophy (BPH) and in 56 control men of various ages. No significant difference was found in T, DHT nor DHT/T ratio between BPH and control subjects of similar age. Plasma DHT was higher in the prostatic than in the peripheral veins in 8/9 patients with BPH during laparotomy, indicating a prostatic secretion of DHT. No difference in the mean T nor the mean DHT was found in peripheral plasma before and after adenomectomy.


BioTechniques ◽  
2021 ◽  
Author(s):  
Oriane Cédile ◽  
Sólja Remisdóttir Veyhe ◽  
Marcus Høy Hansen ◽  
Kjell Titlestad ◽  
Charlotte Guldborg Nyvold

Method summary Concentrations of circulating DNA in blood plasma were compared using NanoDrop, Qubit, quantitative PCR and Bioanalyzer, and DNA integrity was evaluated with the Bioanalyzer according to the time of plasma preparation.


2018 ◽  
Vol 13 (1) ◽  
pp. 163-173 ◽  
Author(s):  
Małgorza Szczuko ◽  
Rafał Migrała ◽  
Arleta Drozd ◽  
Marcin Banaszczak ◽  
Dominika Maciejewska ◽  
...  

AbstractThis study is aimed at determining the content of water soluble vitamins in amateur sportsmen before and after the use of reduction diet. Twenty adult male amateur sportsmen aged between 20 and 43 qualified for this study. The participants adhered to individually adjusted reduction diets for 3 months. Diet supplements were excluded from the diet during that time. Vitamins were analyzed before and after the reduction diet using HPLC. The following vitamins were analyzed: B1, B2, B3, PP, B5, B6, B7, B9, B12 and vitamin C. Statistically significant increase of riboflavin in blood plasma (p=0.0254) and statistically significant (p=0.048) decrease in the concentration of niacin was found when compared to the period before the diet. Appropriate level of riboflavin can be obtained by the consumption of proper amount of diary in the diet. Supplementation with PP is advised and, in case of improper diet, the supplementation of B1, B7 and B12 may also be considered.


1988 ◽  
Vol 118 (2) ◽  
pp. 254-259 ◽  
Author(s):  
Daniel Fröhli ◽  
Jürg W. Blum

Abstract. Experiments were designed to study effects of 3 days of fasting on blood plasma levels, metabolic clearance rates (MCR) and effects of norepinephrine (NE) and epinephrine (E) on levels of glucose, nonesterified fatty acids (NEFA) and immunoreactive insulin (IRI) in 12 steers. During fasting, levels of E, NE and protein did not change, whereas IRI, T3 and glucose decreased and NEFA, acetoacetate and β-hydroxybutyrate increased. Before and at the end of fasting, NE or E were iv infused for 120 min. NE and E were elevated after 15 min and to the end of the infusion. The increase in E, but not in NE, was significantly greater after 3 days of fasting than before fasting (P < 0.05). MCR for E was lower after fasting (299 ± 17 vs 204 ± 10 ml·kg−0.75·min−1; P < 0.001), whereas MCR for NE was not significantly different (455 ± 37 vs 400 ±27 ml·kg−0.75·min−1). MCR was higher for NE than for E, both before and after fasting (P < 0.05). After the infusions, E and NE decreased within minutes to pre-infusion concentrations. During E infusions, NEFA increased significantly more, whereas glucose increased less in fasted than in fed animals. During NE infusions, NEFA increased in fasted, but not in fed animals, and glucose increased less at the end than before fasting. IRI decreased during E infusions only in fed animals, and transiently increased after the infusion, except after NE infusion in fasted steers. Changes in plasma levels, clearance rates and sensitivity to effects of NE and E, together with alterations of insulin and T3 concentrations, may contribute to shifts in energy metabolism during food restriction.


1976 ◽  
Vol 83 (4) ◽  
pp. 856-864 ◽  
Author(s):  
Pentti Holma ◽  
Herman Adlercreutz

ABSTRACT Plasma levels of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) as well as the response of LH and FSH to the intravenous administration of 100 μg of luteinizing hormone releasing hormone (LRH) were measured in 16 well-trained athletes (mean age 30 years) before and after 2 months of daily oral intake of 15 mg of metandienon, an anabolic steroid (Anabolin®, 17α-methyl-17β-hydroxy-1,4-androstadien-3-one, Medica, Finland). All athletes continued to train regularly, just as they had done for several years. During administration of metandienon the mean plasma testosterone level fell 69%, from 29.4 ± 11.6 nmol/l to 9.1 ± 7.5 nmol/l. The mean plasma levels of LH and FSH also fell significantly (P < 0.001 and P < 0.01, respectively), both about 50%. Because LH and FSH levels were low after administration of the steroid the maximum stimulation values after LRH administration were also lower than pre-treatment values although the mean increments did not differ significantly before and after administration of the anabolic steroid. However, after treatment, the FSH response curve had a biphasic pattern in most subjects, with peaks at 10 to 20 and 50 to 60 min after the iv injection of LRH. Administration of LRH after the treatment period had no effect on FSH secretion in two subjects and no effect on LH secretion in one. Our results show that administration of an anabolic steroid causes a pronounced lowering of plasma levels of testosterone, LH and FSH but causes no gross alteration in the response of LH secretion to stimulation by LRH. The reason for the biphasic response pattern of FSH to LRH administration in most subjects is not known.


2017 ◽  
Vol 71 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Grzegorz Słomian ◽  
Elżbieta Świętochowska ◽  
Grzegorz Nowak ◽  
Krystyna Pawlas ◽  
Aleksandra Żelazko ◽  
...  

Adipokines are molecules produced and secreted by adipose tissue and are linked to multiple malignancies. Adipokines can suppress or promote particular cell behaviors in different types of cancer. The aim of this study was to investigate the impact of chemotherapy on select adipokines in patients with colorectal cancer (CRC).Blood samples were collected from 42 patients with pathologically documented advanced CRC, who required palliative chemotherapy. Leptin, adiponectin, resistin and visfatin levels were measured by ELISA before and 3 months after the administration of chemotherapy. Among the 42 patients evaluated, 18 achieved a partial response (PR), 16 achieved stable disease (SD) and 8 patients experienced disease progression (PD).We found that 5-fluorouracil-based chemotherapy regimens significantly increased plasma levels of leptin and adiponectin and decreased plasma levels of resistin and visfatin in PR and SD patients, whereas the plasma levels of these molecules were not affected in PD patients. Furthermore, the mean plasma levels of leptin were significantly lower, and the mean plasma levels of resistin and visfatin were significantly greater in patients with PD compared with PR and SD both before and after chemotherapy treatment.We conclude that palliative chemotherapy in CRC patients, in addition to providing clinical benefits, positively affects cytokine production and secretion in PR and SD patients. Specifically, we found that palliative chemotherapy increased plasma levels of the anti-inflammatory adipokine adiponectin and decreased the plasma levels of visfatin and resistin, molecules known to promote angiogenesis and cancer cell proliferation in PR and SD patients. Moreover, the baseline values of leptin, visfatin and resistin might serve as prognostic indicators of a poor response to chemotherapy.


1986 ◽  
Vol 156 (3) ◽  
pp. 315-320 ◽  
Author(s):  
Y. Mauras ◽  
K.S. Ang ◽  
P. Simon ◽  
B. Tessier ◽  
F. Cartier ◽  
...  

2018 ◽  
Vol 2 (2) ◽  
pp. 101-108
Author(s):  
Defrin Defrin ◽  
Rosfita Rasyid

Premature rupture of membranes is the most common complication of pregnancy. The incidence of premature rupture of membranes in pregnancy ranged from 6% to 10%, and 20% of these cases occur before 37 weeks gestation. The incidence of premature rupture of membranes in Indonesia ranges from 4.5% to 7.6% of all pregnancies. This research was conducted to determine the cross-sectional differences in the blood plasma levels of vitamin C in term pregnancy premature rupture of membranes with blood plasma levels of vitamin C in term pregnancy without premature rupture of membranes in M. Jam- il Padang hospital, Achmad Muchtar Bukittinggi hospital, and Pariaman Hospital. There are significant differences in vitamin C blood plasma levels in term pregnancy with premature rupture of membranes and term pregnancy without premature rupture of membranes ( P < 0.05). Mean levels of vitamin C in blood plasma at term pregnancy with premature rupture of membranes lower than in the blood plasma levels of vitamin C in term pregnancy without premature rupture of membranes.Keywords: Premature rupture of membrane in aterm, blood plasma levels of vitamin C


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