EFFECT OF METHIONINE SUPPLEMENTATION POSTERIOR TO THE RUMEN ON NITROGEN UTILIZATION AND SULFUR BALANCE OF STEERS ON A HIGH ROUGHAGE RATION

1970 ◽  
Vol 50 (2) ◽  
pp. 319-324 ◽  
Author(s):  
G. STEINACKER ◽  
T. J. DEVLIN ◽  
J. R. INGALLS

The effect of post ruminal methionine, dietary methionine or dietary sulfur (S) supplementation on nitrogen retention and S excretion was determined by using three Holstein steers fitted with abomasal cannula in a 3 × 3 Latin square design. The basal diet was fed ad libitum and consisted of ground timothy hay and concentrate in a 5:1 ratio. The diet contained 12% crude protein with urea contributing about 40% of the total nitrogen. Elemental S (0.05%) or methionine (0.22%) was added to the diet while a similar quantity of methionine was infused into the abomasum. Abomasal infused methionine compared with supplemental dietary S or methionine resulted in a 16.6 and 23.9% increase in percent of absorbed nitrogen retained, which was not statistically significant (probability approximately 0.25). Sulfur intake and apparent S digestibility was similar for all treatments; however, urinary S concentration tended to be lower for the elemental S treatment (probability approximately 0.10). Sulfur retention was higher (P < 0.05) with supplementary elemental S than when a similar amount of S was consumed or infused in the form of methionine.

1983 ◽  
Vol 63 (3) ◽  
pp. 613-621 ◽  
Author(s):  
L. E. PHILLIP

Eight wether lambs, four equipped with rumen cannulae and four intact, were assigned to four high-concentrate diets according to a 4 × 4 double latin square with 27-day periods. The basal diet consisted of barley and corn silage, the latter representing 30% of the dry matter (DM), and was either unsupplemented (9% dietary protein) or supplemented (15% protein) with brewers' dried grains. To each of the low protein (LP) and high protein (HP) diets, sodium bicarbonate (NaHCO3) was either not added or added at the level of 4% of the diet DM. There was a marked, though not statistically significant, improvement in nitrogen retention due to NaHCO3 inclusion, particularly when protein intake was low. Voluntary feed intake increased by 11% as a result of NaHCO3 addition but the effect was nonsignificant. Bicarbonate-induced changes in feed intake were unrelated to changes in rumen pH. A maximum ruminal osmolality of 303 mOsm/kg elicited by NaHCO3 addition did not appear to restrict feed intake. Rumen ammonia concentration tended to decrease as a result of NaHCO3 addition. Digestibility of organic matter and of DM was unaffected by the inclusion of NaHCO3 but digestibility of nitrogen tended to increase. It is suggested that NaHCO3 could have a protein-sparing effect when added to protein-deficient concentrate diets, and that bicarbonate-induced changes in feed intake may be related to its influence on protein status of ruminants. Key words: Bicarbonate, buffers, lambs, nitrogen utilization, feed intake


1984 ◽  
Vol 39 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Jane Leibholz

ABSTRACT1. Forty entire male pigs between 7 and 28 days of age and forty entire male pigs between 28 and 56 days of age were fed ad libitum basal diets of wheat and lupins containing 2·4 and 1·9 g of methionine per kg respectively. These were supplemented with 0, 0·3, 0·6, 0·9 and 1·2 g of synthetic methionine per kg of diet.2. The methionine required for maximum performance and nitrogen retention was 3·0 g/kg of dry matter between 7 and 28 days of age and 2·5 g/kg between 28 and 56 days of age. These values were equivalent to 1·14 and 1·19g/100g of dietary crude protein at the two ages. The cystine contents of the diets were 3·7 and 3·1 g/kg respectively.3. The concentration of methionine in the blood plasma did not give a clear indication of methionine requirement.


2008 ◽  
Vol 15 (3) ◽  
pp. 235 ◽  
Author(s):  
P. PURSIAINEN ◽  
M. TUORI

The effect of replacing wilted grass silage (GS) with pea-barley intercrop silage (PBS) on feed intake, diet digestibility and milk production was studied with 8 multiparous Ayrshire-cows in a replicated 4 × 4 Latin square experiment. Proportion of PBS was 0 (PBS0), 33 (PBS33), 67 (PBS67) or 100 (PBS100) % of silage dry matter (DM). The DM content was 559 and 255 g kg-1 for GS and PBS. Crude protein content was 131 and 170 g kg-1 DM, respectively. Pea-barley silage was more extensively fermented than GS with total fermentation acid content of 120 vs. 12 g kg-1 DM. Silage was fed for ad libitum intake and supplemented with on the average 13 kg concentrate per day. Silage DM intake was 9.2 (PBS0), 9.7 (PBS33), 9.0 (PBS67) and 7.1 (PBS100) kg per day (Pquadr. < 0.05). The energy corrected milk yield [30.3 (PBS0), 29.8 (PBS33), 30.3 (PBS67), 31.3 (PBS100) kg per day] was not significantly affected by the treatment. Milk protein concentration decreased linearly (P < 0.05) in response to feeding PBS. It is concluded that PBS can replace up to two thirds of wilted, moderate quality GS in the feeding of dairy cows because in this experiment pure pea-barley silage reduced silage intake.


2021 ◽  
Vol 1 (2) ◽  
pp. 184-191
Author(s):  
V. A. Oyenuga ◽  
B. L. Fetuga

The proximate and mineral constituents of six plantain forms, raw green plantain (RGP), cooked green plantain (CGP), raw ripe plantain (RRP), cooked ripe plantain (CRP), green plantain meal (GPM) and ripe plantain meal (RPM) were determined by chemical analysis. 24 large white barrows, weighing initially, between 40-45 kg., were used to study the apparent digestibility of nutrients, digestible energy (DE), metabolizable energy (ME), metabolizable energy corrected for nitrogen retention (ME(n) and total digestible nutrients when the plantains served as the only source of nutrients. In a second experiment ME and ME(n) were measured for the plantain forms using 28 Large White barrows, with diets in which the plantains replaced 50% of maize in a basal diet. All the plantain forms had low fat, crude fibre and crude protein content but high in nitrogen-free extract, phosphorus, potassium and Iron. Identically high dry matter (D.M.) and nitrogen-free extract (N.F.E.) digestion coefficients were obtained for all the plantain forms except RPM, which had significantly lower values. Digestion coefficients for crude protein (46.9, 53.6, 51.8, 34.3,-32.7 %), crude fibre, (46.6, 49.5, 58.7, 62.6, 56.9, 39.6 %), ether extract (55.9, 56.6, 66.9, 69.2, 54.7 and 34.6 %) for RGP, CGP, RRP, CRP, GPM and RPM respectively were considerably lower than for D.M. and N.F.E. The DE, ME ME(n) and TDN values were comparable for all plantain forms except RPM, which had significantly lower values in most cases. The fresh green plantains and green plantain meals had slightly higher energy values than the ripe plantains, even though the nutrients in the ripe plantains were better digested than in the green forms. The results are discussed in relation to variations in the relative proportions of the simple and more complex carbohydrates during ripening.


1997 ◽  
Vol 1997 ◽  
pp. 85-85
Author(s):  
J. N. Methu ◽  
E. Owen ◽  
A. Abate ◽  
M. Scarr ◽  
J. Tanner

Several studies with barley straw (e.g. Wahed et al, 1990) and sorghum stover (e.g. Osafo, 1993) have shown improvements in intake with increasing ‘ad libitum’ amounts offered. Furthermore, these studies have demonstrated that sheep and goats offered barley straw or sorghum stover in long, unprocessed form, increase intake by selecting for leaf and leaf sheath, and against stem. This phenomenon offers a simple feeding strategy to use selective feeding behaviour to improve intake and hence production. The aim of the present experiment was to study the effect of offering increasing amounts of long maize stover on intake and selection by dairy cows.Six, early- to mid-lactation Ayrshire and Friesian cows (live weight, M, 430 kg) were used in a double 3x3 Latin square design with 24-day (d) feeding periods. Cows were offered long (unchopped) maize stover at low, medium or high ‘ad libitum’ rates, i.e. 31, 59 or 87 g dry matter (DM)/kg M.d. Cows also received 3.2 kg DM/d of cotton seed cake (339 g/kg DM crude protein) in two meals at milking (0700 h and 1800 h). Stover was offered in one meal daily, at 0700 h, after collecting refusals from the previous day. Amounts of stover offered and refused were weighed daily. Samples of offered stover (0.5 kg) and refused stover (0.5 kg) were analysed for DM daily. All refused stover and 4.0 kg samples of offered stover were botanically fractionated, daily, into stem (S), leaf (L), sheath (Sh) and husk (H). Milk yield was recorded daily and cows weighed at the start and end of each period.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 229-229
Author(s):  
Bokyung Hong ◽  
Beob G Kim

Abstract Two experiments were conducted to investigate the effects of phytase source and feed pelleting on digestibility of dry matter (DM), crude protein, organic matter (OM), and phosphorus (P) with different doses of phytase in pigs. In Exp. 1, ten barrows (31.4 ± 3.5 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 6 Latin square design with 5 diets and 6 periods. In Exp. 2, ten barrows (28.2 ± 3.2 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 5 Latin square design with 5 diets and 5 periods. Each period lasted 10 d consisted of a 5-d adaptation and a 5-d collection periods and the marker to marker method was used for fecal collection. In both experiments, a basal diet was prepared based on corn and soybean meal without phytase or inorganic P. Four additional diets were prepared in a 2 × 2 factorial treatment arrangement with 2 phytase sources (Buttiauxella spp. or Escherichia coli) and 2 feed forms (mash or pellet). Both experiments employed the same treatments but had different supplemental phytase doses (500 and 1,500 FTU/kg in Exp. 1 and 2, respectively). In Exp. 1, supplemental phytase increased standardized total tract digestibility of P (66.1 vs. 51.7%; P &lt; 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM, crude protein, and OM (P &lt; 0.001). In Exp. 2, supplemental phytase increased standardized total tract digestibility of P (72.8 vs. 44.8%; P &lt; 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM and OM (P &lt; 0.001). Taken together, supplemental phytase at 500 and 1,500 FTU/kg increases P digestibility regardless of phytase source or feed pelleting.


1976 ◽  
Vol 22 (3) ◽  
pp. 419-421 ◽  
Author(s):  
G. M. J. Horton ◽  
W. Holmes

SUMMARYSix castrated male cattle of 350 kg live weight were used in an incomplete Latin square design to measure intake and digestibility of barley straw offered ad libitum alone and with 5 levels, 1·5 to 7·5 kg/day, of a barley and dried lucerne concentrate. Straw intake declined and total organic-matter intake increased linearly with increasing concentrate level. On average 1 kg additional organic matter as concentrate, increased total organic-matter intake by 0·68 kg. There was no evidence that the crude protein content of the whole diet affected straw consumption.


1976 ◽  
Vol 16 (82) ◽  
pp. 661 ◽  
Author(s):  
NJ Barton ◽  
JW McLaughlin

Pasture hay of 18 per cent crude protein content was chaffed and sprayed with an 8 per cent solution of formaldehyde in a concrete mixer. This, or similar untreated chaffed hay was fed to Merino weaner sheep in four experiments as follows: 1. Twenty-four sheep grazing in a 0.9 ha paddock were penned individually three times a week and fed 700 g of either treated or untreated hay. During the intervening periods they were released and allowed to graze. 2. Twelve sheep penned indoors were given 300 g day-l of either treated or untreated hay as a supplement to a basal diet of hay which contained nine per cent crude protein. 3. Twelve sheep penned indoors were fed either treated or untreated hay ad libitum. 4. Six sheep in metabolism cages were fed either treated or untreated hay at a daily rate of 52 g kg-I liveweight0.75 A crossover experimental design was used. Wool production, body growth and, in experiment 4,nitrogen balance were recorded. Attempts were also made to gauge the effectiveness of the treatments in protecting the protein from ruminal degradation. Although the level of protection obtained compared favourably with that obtained by other workers with forages, significant differences in animal production were shown only in experiment 2, where the basal hay was utilized less efficiently for body growth when the supplement was treated, in experiment 3. where treated hay was utilized less efficiently for body growth, and in experiment 4, where wool growth was increased by 10 per cent and nitrogen retention was also increased by treatment of the hay. It was concluded that the magnitude of any likely beneficial response to treatment is too small to warrant this treatment of hay being undertaken on a large scale.


2021 ◽  
Vol 9 (1) ◽  
pp. 11-20
Author(s):  
O. A. Ikuwegbu

Four calves equipped with permanent rumen and T-piece duodenal caninulus were given four diets in a Latin square experiment carried out at two stages of development. DM flow was measured by dual-phase markers. The basal diet of hay and concentrate was calculated to be low In rumen degradable N (RDN) and. tissue N. Additional RDN was provided by ad­ding 6, 12 or 18g urea/kg concentrate. Supplementary urea did not affect OM digestion either in the stomach or In the entire digestive tract. At the older age OM digestion in the stomach and the entire tract was significantly higher. On the basal diet, N retention was low. The flow of microbial N measured by the DAPA technique was not affected by diet and it was calculated that between 3 and 7g "s/d were recycled to the rumen. The addition of supplementary urea increased N retention par­ticularly at the lowest level of supplementation due to a concomitant decrease In urinary N.


1988 ◽  
Vol 60 (7) ◽  
pp. 711-721
Author(s):  
Pekka Huhtanen ◽  
Hannele Ala-Seppälä ◽  
Matti Näsi

A duplicated 4 x 4 Latin Square experiment was conducted to determine the effects of a gradually increased level of barley fibre in the diet on ad libitum grass silage intake and milk production. Barley fibre is a fibrous ethanol-starch by-product (120 g crude protein, 550 g neutral detergent fibre (NDF) and 120 g starch/kg dry matter (DM)). The four supplements, given at the rate of 6.5 kg/d on DM basis, were barley (B) and barley of which 333 g/kg (BF), 667 g/kg (FB) and 1000 g/kg (F) were replaced by barley fibre. In addition the cows were given 1 kg of rapeseed meal and 0.25 kg of mineral mixture. As the proportion of barley fibre in the diet increased there was a linear (P


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