BLOAT INVESTIGATIONS. STUDIES ON SOLUBLE PROTEINS AND NUCLEIC ACIDS IN BLOATING AND NONBLOATING FORAGES

1969 ◽  
Vol 49 (1) ◽  
pp. 69-75 ◽  
Author(s):  
J. M. McArthur ◽  
J. E. Miltimore
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Total Protein ◽  
Acid Content ◽  
Soluble Proteins ◽  
Nucleic Acid Content ◽  
Total Proteins ◽  
Agar Gel ◽  
Forage Mixtures

The soluble proteins in five bloating and six nonbloating forages were separated chromatographically on agar gel columns and 18S protein, the agent causing bloat, was determined in column effluents. Bloating forages contained approximately one-third of total proteins in the form of 18S; nonbloating forages one-sixth or less. Total protein was highest in bloating forages. Nucleic acid content of the two types of forages was not significantly different. Production of nonbloating forage mixtures by mixing forages of high and low 18S protein is discussed.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

scholarly journals Studies on the Nucleic Acids of the Malaria Parasite, Plasmodium Berghei (Vincke & Lips)

1953 ◽  
Vol 6 (2) ◽  
pp. 234 ◽  
Author(s):  
PR Whitfeld
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Acid Content ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Nucleic Acid Content ◽  
Solid Residue ◽  
Parasite Plasmodium

Changes in� the nucleic acid content of the solid residue obtained by haemolysing the blood of mice infected with Plasmodium berghei have been examined. The residue from blood in which 25 per cent. of the red blood cells were parasitized contained 20-25 times as much ribosenucleic acid (RNA) and 12 times as much desoxyribosenucleic acid (DNA) as the residue from uninfected blood.

scholarly journals Nucleic Acid Content in Different Tissues of the Fish N. notopterus in Relation to Sex

2015 ◽  
Vol 34 ◽  
pp. 1-6
Author(s):  
K. Ravikiran ◽  
R.S. Kulkarni
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Freshwater Fish ◽  
Reproductive Cycle ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Female Fish ◽  
Male Fish ◽  
Different Tissues

Nucleic acid content in different tissues in the freshwater fish N.notopterus in relation to sex was studied during Pre-spawning phase of the reproductive cycle. The fish were scarified and the tissues such as brain, liver, kidney and gonads removed and processed for determination of nucleic acids. It is observed that the nucleic acid content in different tissues of female fish has lower values than males. The results obtained in the present study may provide a contribution to the knowledge of the characteristics of nucleic acid as parameters of sexing the fish. The study also indicates that although male fish have higher values than female fish, both the sexes are biochemically and nutritionally better.

Application of Ogur & Rosen's Method for the Determination of Nucleic Acid Content of Bacteria

1957 ◽  
Vol 12 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Nobuyasu KAWASAKI ◽  
Ichiro TAKI ◽  
Chiaki WATANABE ◽  
Kiyoshi MATOBA ◽  
Mokichiro NISHIO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

Age-Related Effect of Dietary Nucleotides on Liver Nucleic Acid Content in Rats

1997 ◽  
Vol 41 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Ana T. López-Navarro ◽  
Angel Gil ◽  
Antonio Sánchez-Pozo
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Related Effect ◽  
Age Related ◽  
Dietary Nucleotides
Sign in / Sign up

Export Citation Format

Share Document