SYNCHRONIZATION OF ESTRUS AND ARTIFICIAL INSEMINATION IN BEEF CATTLE

1968 ◽  
Vol 48 (3) ◽  
pp. 303-306 ◽  
Author(s):  
R. D. Baker ◽  
E. G. Coggins
Keyword(s):  
Beef Cattle ◽  
Artificial Insemination ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Post Treatment ◽  
Feeding Period ◽  
Treatment Service ◽  
Time Required

Fifty Aberdeen Angus cows were group-fed 1 kg of meal containing 10 mg of 6-chloro-Δ6-17 acetoxyprogesterone (CAP) per female per day for 14 days, followed by 1 kg of meal containing 5 mg of CAP per female per day for 4 days. Twenty-five cows were injected with 1,000 IU of human chorionic gonadotrophin (HCG) 48 hr after the last CAP feeding. The cows injected with HCG were artificially inseminated 12–24 hr after the injection. Non-injected cows were inseminated approximately 12 hr after the onset of standing heat. CAP was successful in suppressing estrus in 47 (94%) of the cows during the feeding period. Seven of the 20 non-injected cows which showed heat conceived to the first post-treatment service. Six of the 15 HCG-injected cows which showed heat and four of the 10 HCG-injected cows which did not show heat conceived to the first post-treatment service. Of the 27 cows which showed a second post-treatment estrus and were inseminated, 13 cows conceived, giving a total of 30 cows conceiving to the artificial insemination services. The HCG injection had no significant (P > 0.05) effect on the incidence of estrus or conception; however, it did eliminate the problem of detecting estrus and significantly (P < 0.01) reduced the time required for restraining and inseminating the cows.

SIMULTANEOUS RADIOIMMUNOASSAY OF LUTEINIZING HORMONE AND FOLLICLE-STIMULATING HORMONE

1978 ◽  
Vol 79 (3) ◽  
pp. 407-408 ◽  
Author(s):  
M. J. ELLIS ◽  
R. A. DONALD ◽  
J. H. LIVESEY
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Significant Proportion ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Operating Costs ◽  
Two Factors ◽  
And Performance ◽  
Time Required ◽  
Stimulating Hormone

The Medical Unit, The Princess Margaret Hospital, Christchurch 2, New Zealand (Revised manuscript received 21 August 1978) The frequent clinical and research requirement for measurement of both plasma luteinizing hormone (LH) and follicle-stimulating hormone (FSH) has prompted the development of a simultaneous radioimmunoassay for these two hormones. The considerable advantages of a simultaneous method include an economy of plasma volume, assay reagents, test-tubes and, more importantly, the time required for radioactive counting and performance of the assay by technical staff. The latter two factors comprise a significant proportion of radioimmunoassay operating costs. This report describes a simultaneous radioimmunoassay based on the use of 131I-labelled FSH, 125I-labelled LH, anti-FSH serum M93 6873 (a generous gift from Professor W. R. Butt, Birmingham), anti-human chorionic gonadotrophin (HCG) serum for LH measurement (Donald, 1972) and donkey anti-rabbit precipitating serum (Wellcome Reagents, U.K.) for separation of antibody-bound and free hormones. Pituitary gonadotrophin standard (LER 907)

Control of the time of ovulation in sheep

1960 ◽  
Vol 11 (3) ◽  
pp. 389 ◽  
Author(s):  
AWH Braden ◽  
DR Lamond ◽  
HM Radford
Keyword(s):  
Optimal Control ◽  
Breeding Season ◽  
Artificial Insemination ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Treatment Schedule ◽  
Pregnant Mare Serum Gonadotrophin

Synchronization of ovulation in ewes during the breeding season was achieved by a 14 day course of progesterone injections followed by injections of pregnant mare serum gonadotrophin (PMS) and human chorionic gonadotrophin (HCG). Optimal control was attained when the PMS was given at the time of the last progesterone injection and the HCG 24 hr later. With this treatment most ovulations occurred between 20 and 28 hr after the HCG injection. However, none of the ewes exhibited oestrus at this time. Greater variability in the time of ovulation was found when the progesterone-PMS and PMS-HCG intervals were 0 hr and 48 hr, or 24 hr and 24 hr, and with these treatments about 80 per cent. of the ewes exhibited oestrus just before ovulation. On the 0-48 hr treatment schedule with artificial insemination 3-7½ hr after the HCG injection, 13 of 22 ewes had fertilized eggs or embryos when killed 3 or 35 days later.

scholarly journals Relationships of changes in B-mode echotexture and colour-Doppler signals in the wall of the preovulatory follicle to changes in systemic oestradiol concentrations and the effects of human chorionic gonadotrophin in mares

Reproduction ◽  
2006 ◽  
Vol 131 (4) ◽  
pp. 699-709 ◽  
Author(s):  
E L Gastal ◽  
M O Gastal ◽  
O J Ginther
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Post Treatment ◽  
Arterial Blood Flow ◽  
Preovulatory Follicle ◽  
Colour Doppler ◽  
Arterial Blood ◽  
Grey Scale ◽  
Doppler Signals ◽  
Negative Effect

A duplex grey-scale and colour-Doppler ultrasound instrument was used to study the changes in the wall of the preovulatory follicle in mares. When the follicle reached ≥35 mm (hour 0), mares were randomized into control (n= 16) and human chorionic gonadotropin (hCG)-treated (n= 16) groups. The hCG treatment was given at hour 0. Scanning was done every 12 h until hour 36, every hour between hours 36 and 48, and every 12 h thereafter until ovulation. Blood was sampled every 12 h for oestradiol assay. During the period 0–24 h post-treatment, oestradiol concentrations decreased in the hCG group and increased in the controls (significant interaction). During the period 0–36 h post-treatment, thickness and echogenicity of the granulosa increased in the hCG group but not in the controls. During the period 36 to 12 h before ovulation, granulosa and colour-Doppler end-points increased in the control and hCG groups (hour effects), while oestradiol was decreasing in both groups. The prominence and percentage of follicle circumference with an anechoic band peripheral to the granulosa and colour-Doppler signals in the follicle wall, indicating arterial blood flow, decreased during the period 4 to 1 h before ovulation (hour effects). Results indicated that the ultrasonographic changes of the wall of the preovulatory follicle were not associated temporally with changes in oestradiol concentrations and prominence of an anechoic band, and colour-Doppler signals decreased during the few hours before ovulation. The hypothesis that the latter portion of the ovulatory LH surge has a negative effect on systemic oestradiol was supported by the immediate decrease in oestradiol concentrations when hCG was injected.

scholarly journals THE EFFECTIVENESS OF PROGESTERONE HORMONE OR PGF2α AND hCG APPLICATION TO INCREASE REPRODUCTIVE EFFICIENCY OF POSTPARTUM OF ANESTRUS PE GOATS

2018 ◽  
Vol 11 (4) ◽  
Author(s):  
Muhammad Syawal ◽  
Tuty L Yusuf ◽  
Muhammad Agil
Keyword(s):  
Drug Release ◽  
Artificial Insemination ◽  
Clinical Signs ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Reproductive Efficiency ◽  
Reproductive Condition ◽  
Hormone Administration

This study aimed to improve reproductive condition of postpartum anestrus of Etawa crossbreed (PE) goats using PGF2α hormone or progesterone (controlled internal drug release/CIDR-G) and improve pregnancy success from artificial insemination (AI) with human chorionic gonadotrophin (hCG) hormone administration. Administration of progesterone increased estrus by 100%, which was higher than the 70% produced from prostaglandin F2 alfa (PGF2α) administration. Estrus onset in CIDR group (35.80±13.33 hours) was faster than PGF2α group (45.6±9.2 hours). Clinical signs of vulva (redness and swollen) were more intense in CIDR-G group (80% and 70%, respectively) compared to PGF2α group (70% and 57%, respectively). Estrus duration in PGF2α group (52.17±3.34 hours) was longer than CIDR-G group (49.61±3.56 hours). Pregnancy success in group given hCG was 66.7% while in non-hCG group was 50%. In conclusion, CIDR-G and PGF2α administration is effective to induce estrus of postpartum anestrus PE goats. Administration of hCG at the onset of estrus is effective to improve pregnancy success.

Hormonal synchronization of ovulation in beef heifers and fertility after artificial insemination

1964 ◽  
Vol 15 (3) ◽  
pp. 461
Author(s):  
DR Lamond
Keyword(s):  
New England ◽  
Corpus Luteum ◽  
Artificial Insemination ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Beef Heifers ◽  
Final Injection ◽  
Pregnant Mare Serum Gonadotrophin

After ovarian examination, and enucleation of corpora lutea where possible, groups of beef heifers on farms in the New England region were injected with progesterone in oil for 6 days. This was followed in some experiments by treatment with placental gonadotrophins. Artificial insemination was carried out 3–5 days after the final injection of progesterone. The effects of dose of progesterone, pregnant mare serum gonadotrophin, and human chorionic gonadotrophin on fertility were observed. Fertility was highest in heifers in which the corpus luteum was enucleated prior to injection of progesterone.

scholarly journals Inducing Ovulation with hCG Improves Fertility Outcomes of Co-Dominant Follicle Drainage to Avoid Twin Pregnancy in Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 169
Author(s):  
Irina Garcia-Ispierto ◽  
Mònica Pando ◽  
Mònica Llobera-Balcells
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Pregnancy Loss ◽  
Twin Pregnancy ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Dominant Follicle ◽  
Drainage Procedure ◽  
Twin Pregnancies ◽  
Clinical Problems

Twin pregnancies are undesirable in dairy cattle as they dramatically compromise cow lifespan and, consequently, herd economy. Clinical problems in cows arise from the time of pregnancy diagnosis to pregnancy loss, abortion, or parturition. The drainage of co-dominant follicles in cows with two or more follicles at insemination prevents twin pregnancy. The objective of this study was to compare the effectiveness of draining the smaller of two follicles through a simplified maneuver not requiring ultrasonography in cows in their third or more lactation, and then inducing ovulation immediately before artificial insemination (AI) with GnRH or human chorionic gonadotrophin (hCG). Animals were monitored by ultrasound at AI and randomly assigned to the groups: follicular drainage and treatment with GnRH (Deph; n = 60), follicular drainage and treatment with hCG (hCG; n = 60), and non-drainage (ND; n = 60) as control cows. On the basis of odds ratios, cows treated with hCG were 2.1 times more likely to become pregnant than control animals. Our results reveal the efficacy of hCG treatment at AI in cows with two follicles of pre-ovulatory size subjected to a simple follicular drainage procedure.

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