RUMEN GAS ANALYSIS BY GAS-SOLID CHROMATOGRAPHY

1961 ◽  
Vol 41 (2) ◽  
pp. 187-196 ◽  
Author(s):  
J. M. McArthur ◽  
J. E. Miltimore
Keyword(s):  
Carbon Dioxide ◽  
Carbon Monoxide ◽  
Hydrogen Sulphide ◽  
Sample Volume ◽  
Gas Analysis ◽  
Complete Analysis ◽  
Thermal Detector ◽  
Thermal Detection ◽  
Methane Carbon

Methods are described for sampling and analysing rumen gases. The analysis requires less than 15 minutes for the determination of hydrogen, oxygen, nitrogen, methane, carbon monoxide, carbon dioxide, and hydrogen sulphide, i.e., for all gases occurring in the rumen. The method is sensitive and requires only a small quantity of sample, and the sample volume need not be known. The presence of water or other vapours in the sample does not influence the results. Relative thermal detector responses have been determined for gases which occur in the rumen. These eliminate the necessity for the calibration of gas chromatographs using thermal detection. The first complete analysis of rumen gas is presented.

scholarly journals Research of the utilization of biotechnological agents for the reduction of ammonia and greenhouse gases emissions in livestock breeding in the Czech Republic

2008 ◽  
Vol 53 (No. 4) ◽  
pp. 126-133 ◽  
Author(s):  
A. Jelínek ◽  
M. Dědina ◽  
R. Kraus
Keyword(s):  
Carbon Dioxide ◽  
Czech Republic ◽  
Greenhouse Gases ◽  
Hydrogen Sulphide ◽  
The Czech Republic ◽  
Livestock Breeding ◽  
Greenhouse Gases Emissions ◽  
Poultry Breeding ◽  
Methane Carbon

The reduction of ammonia and greenhouse gases emissions resulting from the livestock breeding is conditioned by the performance of many experiments for the reducing technologies verification. The utilisation of biotechnological agents in the livestock breeding enables to reduce not only ammonia but in many cases also the principal greenhouse gases. In the paper is presented the system and methodology of the measurements, the choice of more than eighty authorised measurements, and the determination of the emission factors for methane, carbon dioxide, hydrogen sulphide, and nitrogen oxide from pig and poultry breeding.

scholarly journals Cave Air Analysis with Gas Chromatography Mass Spectrometry

2021 ◽  
Author(s):  
Gyula Nyerges ◽  
Dénes Szieberth ◽  
Judit Mátyási ◽  
József Balla
Keyword(s):  
Mass Spectrometry ◽  
Carbon Dioxide ◽  
Gas Chromatography ◽  
Carbon Monoxide ◽  
Stationary Phase ◽  
Gas Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Cave System ◽  
Validation Parameters

Gas chromatography (GC) is a frequently used analytical method for the determination of permanent and organic air components. The analysis usually needs two different columns in practice. The molecular sieve stationary phase can separate oxygen, nitrogen and carbon monoxide, but irreversibly adsorbs carbon dioxide and water. Porapak type columns are applicable for the measurement of carbon dioxide, however oxygen, argon, nitrogen and carbon monoxide are co-eluted. Usually these two types of columns are used in parallel for the determination. Carboxen stationary phase can separate carbon monoxide and carbon dioxide, but argon, oxygen and nitrogen are co-eluted. Thermal conductivity detector (TCD) and flame ionization detector (FID) are used commonly together for the determination of the separated components. TCD is applied for permanent gas analysis whereas FID – combined with a methanizer – is used for the detection of carbon monoxide, carbon dioxide and light hydrocarbons. Mass spectrometer (MS) is also a potential detector, because the properly chosen fragment ions can increase the selectivity.We developed a method for the determination of air components, using only one column and one detector. This method is suitable for the measurements by combining the advantages of the carboxen column with mass spectrometry. The validation parameters of the method were in the acceptable interval, so this method is able to determine the air components. The application of this technique to the analysis of cave air provided valuable information to the exploration of the Molnár János cave system.

scholarly journals The Use of Cryogenic Temperature Gas Chromatography for the Determination of Carbon Monoxide and Carbon Dioxide in Cigarette Smoke

1972 ◽  
Vol 6 (4) ◽  
Author(s):  
G.P. Morie ◽  
C.H. Sloan
Keyword(s):  
Carbon Dioxide ◽  
Gas Chromatography ◽  
Carbon Monoxide ◽  
Liquid Nitrogen ◽  
Cigarette Smoke ◽  
Cryogenic Temperature ◽  
Chromatographic Method ◽  
Porapak Q ◽  
Gas Chromatographic Method

AbstractA gas chromatographic method for the determination of carbon monoxide and carbon dioxide in cigarette smoke was developed. A column containing Porapak Q packing and a cryogenic temperature programmer which employed liquid nitrogen to cool the column to subambient temperatures was used. The separation of N

Rapid-flow enthalpimetric determination of urea in serum, with use of an immobilized urease reactor.

1976 ◽  
Vol 22 (8) ◽  
pp. 1314-1318 ◽  
Author(s):  
L D Bowers ◽  
L M Canning ◽  
C N Sayers ◽  
P W Carr
Keyword(s):  
Carbon Dioxide ◽  
Measured Temperature ◽  
Serum Urea ◽  
Serum Samples ◽  
Thermal Detector ◽  
Temperature Changes ◽  
Rapid Flow ◽  
Diacetyl Monoxime ◽  
Flow Stream

Abstract We used a differential thermal detector in conjunction with an immobilized urease reactor to determine urea in serum. Samples (120 mul) are introduced into a flow stream and passed through an "adiabatic" column, which is packed with enough insolubilized urease to completely convert urea to ammonia and carbon dioxide. Measured temperature changes are directly proportional to the serum urea concentration. Urea in the presence of protein, bilirubin, and hemoglobin can thus be rapidly, simply, and inexpensively measured. Results correlate well with those obtained by the manual diacetyl monoxime and urease/indophenol methods.

An Extremely Simple and Fast Microlitre Method for the Determination of Total Carbon Dioxide in Biological Samples

1985 ◽  
Vol 22 (5) ◽  
pp. 509-513 ◽  
Author(s):  
G J Van Stekelenburg ◽  
C Valk ◽  
M J G Van Wijngaarden-Penterman
Keyword(s):  
Carbon Dioxide ◽  
Biological Fluids ◽  
Blood Gas Analysis ◽  
Gas Analysis ◽  
Capillary Blood ◽  
Total Carbon ◽  
Blood Gas ◽  
Carbon Dioxide Content ◽  
Simple Method

For those clinical laboratories equipped with a microprocessor-controlled gas analyser, an extremely simple method is described for the determination of the total carbon dioxide content in various biological fluids. Since this method needs only 20 μL of blood plasma or is less dependent on the original total carbon dioxide content, it is especially suited for paediatric purposes. With our procedure the time necessary for one determination equals the time for one capillary blood gas analysis.

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