Short Communication: Ruminal pH profile and feeding behaviour of feedlot cattle transitioning from a high-forage to a high-concentrate diet

2013 ◽  
Vol 93 (4) ◽  
pp. 529-533 ◽  
Author(s):  
L. Holtshausen ◽  
K. S. Schwartzkopf-Genswein ◽  
K. A. Beauchemin
Keyword(s):  
Feeding Behaviour ◽  
Recovery Time ◽  
Short Communication ◽  
Feedlot Cattle ◽  
Meal Size ◽  
Ph Profile ◽  
Ruminal Ph ◽  
Dietary Transition ◽  
Ph Conditions ◽  
High Forage

Holtshausen, L., Schwartzkopf-Genswein, K. S. and Beauchemin, K. A. 2013. Short Communication: Ruminal pH profile and feeding behaviour of feedlot cattle transitioning from a high-forage to a high-concentrate diet. Can. J. Anim. Sci. 93: 529–533. Sixteen ruminally cannulated cattle (384±33.9 kg) were transitioned from a high-forage to a finishing diet over 21 d using three steps (+15% concentrate DM wk−1). Following the initiation of each transition step, mean daily ruminal pH declined and DM intake, meal size and meal duration were reduced. Recovery time to baseline feeding behaviour increased with severity of acidosis. Changes in feeding behaviour can be indicative of sub-optimal ruminal pH conditions during dietary transition. Transition protocols such as more steps, smaller grain increments or shorter initial and longer final steps may aid in reducing the incidence of acidosis.

Performance, feeding behaviour and rumen pH profile of beef cattle fed corn silage in combination with barley grain, corn or wheat distillers’ grain or wheat middlings

2011 ◽  
Vol 91 (4) ◽  
pp. 703-710 ◽  
Author(s):  
L. Holtshausen ◽  
K.A. Beauchemin ◽  
K.S. Schwartzkopf-Genswein ◽  
L.A. González ◽  
T.A. McAllister ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Feeding Behaviour ◽  
Barley Grain ◽  
Corn Silage ◽  
Feed Conversion ◽  
Ph Profile ◽  
Ruminal Ph ◽  
Wheat Middlings ◽  
Distillers Grain ◽  
Dried Distillers Grain

Holtshausen, L., Beauchemin, K. A., Schwartzkopf-Genswein, K. S., González, L. A., McAllister, T. A. and Gibb, D. J. 2011. Performance, feeding behaviour and rumen pH profile of beef cattle fed corn silage in combination with barley grain, corn or wheat distillers’ grain or wheat middlings. Can. J. Anim. Sci. 91: 703–710. This study compared growth performance, feeding behaviour and ruminal pH profile of growing beef heifers fed a total mixed ration (TMR) containing corn silage and either [400 g kg−1 dry matter (DM)] barley grain (CTL), corn dried distillers’ grain with solubles (CDDGS), wheat dried distillers’ grain with solubles (WDDGS) or wheat middlings (WM). Eighty beef heifers (16 ruminally cannulated; 301±34 kg) were blocked by weight and randomly assigned to eight feedlot pens for a 70-d backgrounding study. Pens were randomly assigned to one of four dietary treatments and equipped with the GrowSafe feed intake system for determining individual feed intake and monitoring feeding behaviour. Dry matter intake (DMI) was lower (P<0.01) and average daily gain (ADG) tended to be lower for CTL (P=0.06) heifers as compared with heifers on other treatments. Feed conversion efficiency (i.e., gain to feed ratio; P=0.41) and feeding behaviour and ruminal pH profile measurements (P>0.05) did not differ among treatments. This study illustrates that barley grain can be replaced by corn dried distillers’ grain, wheat dried distillers’ grain or wheat middlings in diets fed to growing beef cattle without compromising feed conversion efficiency, adversely affecting feeding behaviour (e.g., decreased meal frequency and duration) or increasing the incidence of ruminal acidosis.

330 3-nitrooxypropanol Supplementation of a Forage Diet Decreased Enteric Methane Emissions from Beef Cattle Without Affecting Apparent Total-tract Digestibility

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 186-187
Author(s):  
Aklilu Alemu ◽  
Xiu Min Zhang ◽  
Maik Kindermann ◽  
Karen A Beauchemin
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Control Diet ◽  
Nutrient Digestibility ◽  
Ch4 Emissions ◽  
Ruminal Ph ◽  
Enteric Methane ◽  
Ruminant Diets ◽  
Dietary Treatments ◽  
High Forage

Abstract Supplementation of ruminant diets with the investigational methane (CH4) inhibitor 3-nitrooxypropanol (3-NOP; DSM Nutritional Products, Switzerland) is as a promising mitigation strategy. However, most studies have examined high grain or mixed forage-concentrate diets. The objective of this study was to evaluate the effects of supplementing a forage diet with 3-NOP on rumen fermentation, CH4 emissions and apparent total-tract nutrient digestibility. Eight ruminally cannulated beef heifers (BW = 514 kg) were randomly allocated to two treatments in a crossover design with 49-d periods. Dietary treatments were: control, high-forage diet (90% forage DM basis) without 3-NOP; and NOP, control diet supplemented with 150 mg 3-NOP/kg DM. After a 14-d diet adaption, dry matter (DM) intake (DMI) was recorded daily. Rumen contents were collected on days 17 and 28 for volatile fatty acid (VFA) analysis, whereas ruminal pH was continuously monitored from day 20 to 27. Diet digestibility was measured on day 38 to 41 by total collection of feces. Enteric CH4 emissions were measured on days 46 to 49 in chambers. Dry matter intake was lower (P = 0.001) for NOP (10.0 kg/d) as compared with control (10.2 kg/d). However, DM, neutral and acid detergent fiber, and crude protein digestibilities were similar between treatments (P ≥ 0.29). Although total VFA concentration was not affected by treatment (P = 0.19), the reduction in acetate and increase in propionate proportion for NOP lowered (P &lt; 0.001) the acetate to propionate ratio by 18% as compared with control. Mean pH was lower (P = 0.03) for control (6.46) than NOP (6.57). Furthermore, CH4 yield (g/kg DMI) was 21.6% less (P &lt; 0.001) for NOP relative to control (25.5 g/kg DMI). Overall, the results indicate that enteric CH4 emissions were effectively decreased with 3-NOP supplementation of a forage diet without affecting apparent total-tract digestibility of nutrients.

scholarly journals Short communication: Urea hydrolysis in dairy cattle manure under different temperature, urea, and pH conditions

2017 ◽  
Vol 100 (3) ◽  
pp. 2388-2394 ◽  
Author(s):  
L.E. Moraes ◽  
S.A. Burgos ◽  
E.J. DePeters ◽  
R. Zhang ◽  
J.G. Fadel
Keyword(s):  
Dairy Cattle ◽  
Short Communication ◽  
Urea Hydrolysis ◽  
Cattle Manure ◽  
Ph Conditions

scholarly journals Shifting the pH Profile of Aspergillus niger PhyA Phytase To Match the Stomach pH Enhances Its Effectiveness as an Animal Feed Additive

2006 ◽  
Vol 72 (6) ◽  
pp. 4397-4403 ◽  
Author(s):  
Taewan Kim ◽  
Edward J. Mullaney ◽  
Jesus M. Porres ◽  
Karl R. Roneker ◽  
Sarah Crowe ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Animal Feed ◽  
Relative Activity ◽  
Feed Additive ◽  
Ph Optimum ◽  
Ph Profile ◽  
Phytate Phosphorus ◽  
Ph Conditions ◽  
Stomach Ph ◽  
Hydrolysis Of

ABSTRACT Environmental pollution by phosphorus from animal waste is a major problem in agriculture because simple-stomached animals, such as swine, poultry, and fish, cannot digest phosphorus (as phytate) present in plant feeds. To alleviate this problem, a phytase from Aspergillus niger PhyA is widely used as a feed additive to hydrolyze phytate-phosphorus. However, it has the lowest relative activity at the pH of the stomach (3.5), where the hydrolysis occurs. Our objective was to shift the pH optima of PhyA to match the stomach condition by substituting amino acids in the substrate-binding site with different charges and polarities. Based on the crystal structure of PhyA, we prepared 21 single or multiple mutants at Q50, K91, K94, E228, D262, K300, and K301 and expressed them in Pichia pastoris yeast. The wild-type (WT) PhyA showed the unique bihump, two-pH-optima profile, whereas 17 mutants lost one pH optimum or shifted the pH optimum from pH 5.5 to the more acidic side. The mutant E228K exhibited the best overall changes, with a shift of pH optimum to 3.8 and 266% greater (P < 0.05) hydrolysis of soy phytate at pH 3.5 than the WT enzyme. The improved efficacy of the enzyme was confirmed in an animal feed trial and was characterized by biochemical analysis of the purified mutant enzymes. In conclusion, it is feasible to improve the function of PhyA phytase under stomach pH conditions by rational protein engineering.

scholarly journals The use of thiafentanil oxalate and azaperone for reversible immobilisation of African buffalo (Syncerus caffer) within a nature reserve — Short communication

2015 ◽  
Vol 63 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Zoltán Szabó ◽  
Diederik Venter ◽  
Eustatius Luyt ◽  
Cobus Raath
Keyword(s):  
Recovery Time ◽  
Short Communication ◽  
Nature Reserve ◽  
Tuberculin Test ◽  
African Buffalo ◽  
Syncerus Caffer ◽  
High Potency ◽  
Reversal Agent ◽  
Naltrexone Hydrochloride ◽  
The Mean

Although thiafentanil oxalate has been widely used for wildlife immobilisation on different species, no report has been published about its usefulness on African buffalo (Syncerus caffer). Thirty-four African buffaloes were successfully immobilised at Loskop Dam Nature Reserve, South Africa in July 2013. The animals were kept in bomas of the nature reserve. The purpose of the immobilisation was to provide opportunity for microchip implantation, ear tag placement, intradermal tuberculin test and blood sampling. All animals were immobilised with thiafentanil oxalate 6 mg/animal (0.007–0.01 mg/kg) and azaperone 40 mg/animal (0.07–0.04 mg/kg) using Dan-inject darts and gun. The opioid reversal agent naltrexone hydrochloride 60 mg/animal (0.07–0.1 mg/kg) was given intravenously to the ear vein. The mean induction time was 3.9 ± 0.2 min, the recovery time was 1.65 ± 0.87 min. The results of the present study indicate that thiafentanil oxalate, this low-volume, high-potency, reversible drug combined with azaperone provides fast induction and smooth recovery. The authors recommend this drug combination as a reliable immobilising regimen for African buffalo.

scholarly journals Choice feeding in fattening pigs: Effect of diets differing in nutrient density on feeding behaviour and fattening performance

2020 ◽  
Vol 65 (No. 7) ◽  
pp. 247-257
Author(s):  
Josef Pichler ◽  
Christiane Schwarz ◽  
Martin Gierus ◽  
Karl Schedle
Keyword(s):  
Feeding Behaviour ◽  
Energy Levels ◽  
Performance Data ◽  
Meal Size ◽  
Net Energy ◽  
Nutrient Requirements ◽  
Fattening Performance ◽  
Choice Group ◽  
Dietary Treatments ◽  
Choice Feeding

The aim of this study was to determine the proportion of feed consumed by pigs when they had the choice to meet their nutrient requirements offering a low (LND) or a high (HND) nutrient dense diet on animal performance and feeding behaviour. In total 120 barrows and gilts were allotted to three dietary treatments (LND, HND and a feed choice group, FC). Diets were calculated to keep a constant ratio of megajoule net energy (MJ NE) to nutrient standardised ileal digestible (SID) lysine, SID methionine and cysteine, SID threonine, SID tryptophan, Ca, available P and Na. Pigs of the feed choice treatment that could choose between LND and HND chose an energy content between 13.3 and 13.6 MJ ME or rather 10.1 and 10.4 MJ NE. The ratio between LND and HND changed during the growing period to a higher percentage of HND (26.2% : 73.8% in the starter, 22.0% : 78.0% in the grower and 20.0% : 80.0% in the finisher phase). No differences between barrows and gilts were detected regarding the selected diet. As a result, similar zootechnical performance data were observed for HND and FC, whereas LND led to a declined (P &lt; 0.05) performance. Regarding the feeding behaviour no differences in the parameters meal size and daily feeder visits between LND and HND (P &gt; 0.1) were observed. However, within the FC treatment more and greater meals were consumed (P &lt; 0.05) at the HND feeder compared to the LND feeder. Pigs of modern genetics still have the ability to cover their nutrient requirements choosing between diets differing in nutrient density without impairing performance. Furthermore, the results give no indication for the necessity of different energy levels in diets for sexed pigs.

scholarly journals EXTRATO DE MAMONA COMO MANIPULADOR DA FERMENTAÇÃO RUMINAL

Nativa ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 289
Author(s):  
Pâmella Moraes Franco ◽  
Márcia Rodrigues Carvalho Oliveira ◽  
Joao Rafael de Assis ◽  
Jurandy Gouveira Junior ◽  
Rodrigo Nazare Santos Torres ◽  
...  
Keyword(s):  
Gas Production ◽  
Control Treatment ◽  
Energetic Efficiency ◽  
Ruminal Fermentation ◽  
Ruminal Ph ◽  
Dietary Condition ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
High Forage

Objetivou-se investigar os efeitos da adição do extrato de farelo de mamona (EFM) sobre o perfil da fermentação ruminal in vitro em dietas com alto e baixo teor de forragem. Utilizou-se ensaio de incubação ruminal in vitro com dois controles, um negativo (sem aditivo) e um positivo (monensina sódica) e EFM liofilizado (20, 40 e 60 mg/frasco). Em condições de alto teor de forragem na dieta, a adição do EFM aumentou o pH do meio e a concentração de acetato, reduziu a produção de gás, mas não afetou a produção de gás por unidade de matéria seca (MS) digerida em relação ao tratamento controle. Em comparação com monensina sódica, o EFM reduziu as concentrações de propionato e amônia e aumentou a produção de gás por unidade de MS digerida. Em condições de baixo teor de forragem, a adição do EFM reduziu o pH e potencial redox do meio em relação ao tratamento controle. Em comparação com a monensina sódica, o EFM reduziu o pH do meio e a produção total de gás, mas não afetou a produção de gás por unidade de MS digerida. O extrato de farelo de mamona destoxificado não apresenta potencial como manipulador da fermentação ruminal.Palavras-chave: amônia; digestibilidade; eficiência; metano. CASTOR BEAN EXTRACT AS A MANIPULATOR OF RUMINAL FERMENTATION ABSTRACT: Effects of the castorbean meal extract (CME) on ruminal in vitro were investigate in high and low forage diet conditions. For each dietary condition, one in vitro ruminal incubation experiment was conducted in a completely randomized design, with nine repetitions per treatment (three animal inoculum donators and three 48 hors-incubations). In high forage diet, CME increased ruminal pH acetate concentration, reduced gas production, but it did not affect the gas production per unit of digested dry matter (DM), in relation to control treatment. Compare to monensin sodium, CME reduced propionate and ammonia concentrations and increased gas production per unit of digested DM, indicating that CME reduces ruminal energetic efficiency. In low forage diet, CME reduced pH and redox potential compare to control. Compare to monensin sodium, CME reduced pH and gas production, but it did not affect gas production per unit of digested DM. Castorbean meal extract does not present potential as manipulator of the ruminal fermentation.Keywords: ammonia; digestibility; efficiency; methane.

300 Effects of Associating Feed Additives on Rumen Ph Dynamics by Feedlot Cattle Fed a High-concentrate Diet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 163-164
Author(s):  
Maria B Niehues ◽  
Alexandre Perdigão ◽  
Victor Valério de Carvalho ◽  
Tiago S Acedo ◽  
Guilherme S F M Vasconcellos ◽  
...  
Keyword(s):  
Animal Health ◽  
Feedlot Cattle ◽  
Feed Additives ◽  
Adaptation Period ◽  
Time Duration ◽  
Total Period ◽  
Finishing Cattle ◽  
Ruminal Ph ◽  
Rumen Ph ◽  
Randomized Design

Abstract Our objective was to evaluate the effects of feed additives on ruminal pH of finishing cattle fed a 90%-concentrate diet. Twenty four 18-mo Angus-Nellore crossbred bulls (IBW, 456 ± 6,5 kg) were allocated in a completely randomized design to three treatments with eight replicates each, as follows: 1) Control (MON) - Sodium Monensin (26 mg/kg DM, Rumensin, Elanco Animal Health); 2) Crina® RumistarTM (CR) - a blend of essential oils, 90 mg/kg DM + exogenous α-amylase, 560 mg/kg DM) and 3) CR + HyD® (25-hydroxy-vitamin-D3 at 1 mg/animal/d, CRD, DSM Nutritional Products). The ruminal pH and temperature was monitored individually for 98 days, using a wireless bolus, (SmaXtec Animal Care, Austria). Data were analyzed using the Mixed procedure of SAS and means comparison evaluated by Tukey test at P&lt; 0.05. During adaptation period (i.e. first 14 days), bulls fed CR and CRD had increased rumen mean pH (6.40 and 6.36 vs. 6.16; P &lt; 0.01) and minimum pH (5.89 and 5.87 vs. 5.57; P &lt; 0.01) than bulls fed MON. In addition, rumen pH from bulls fed CR spent less time below 6.0 than bulls fed MON (256.07 vs. 452.62 min/d; P = 0.05). Regarding the total period, bulls fed MON had lower mean (6.22 vs. 6.51 and 6.42, P &lt; 0.01) and minimum rumen pH (5.60 vs. 5.92 and 5.85, P &lt; 0.01) than bulls fed CR and CRD. Additionally, feeding MON increased time duration of rumen pH (390.79 min/day, P &lt; 0.01) and had a larger area below 6.0 (81.52 min x pH units/day; P &lt; 0.01). Moreover, the addition of monensin increased pH time duration below 5.8 (161.10 vs. 121.13 and 122.56 min/day; P = 0.02) compared with CR and CRD, and increased ruminal temperature (39.60 vs. 39.51 and 39.5 °C; P &lt; 0.01). We conclude that feeding Crina® RumistarTM and Crina® RumistarTM HyD® increased the rumen pH of bulls.

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