scholarly journals Short Communication: An association analysis between one missense polymorphism at the SREBF1 gene and milk yield and composition traits in goats

2012 ◽  
Vol 92 (2) ◽  
pp. 167-173 ◽  
Author(s):  
A. Manunza ◽  
A. Zidi ◽  
V. M. Fernández-Cabanás ◽  
J. Jordana ◽  
J. Carrizosa ◽  
...  
Keyword(s):  
Association Analysis ◽  
Milk Yield ◽  
Short Communication ◽  
Regulatory Element ◽  
Fatty Acid Content ◽  
Milk Composition ◽  
Functional Change ◽  
Omega 3 ◽  
Coding Region ◽  
Srebf1 Gene

Manunza, A., Zidi, A., Fernández-Cabanás, V. M., Jordana, J., Carrizosa, J., Belaifa, E., Urrutia, B., Polvillo, O., González-Redondo, P., Amills, M. and Serradilla, J. M. 2012. Short Communication: An association analysis between one missense polymorphism at the SREBF1 gene and milk yield and composition traits in goats. Can. J. Anim. Sci. 92: 167–173. Sterol regulatory element binding transcription factor 1 (SREBF1) regulates the expression of genes involved in the biosynthesis of fatty acids and cholesterol. Herewith, we have sequenced the near-complete coding region and part of the 3′UTR of the goat SREBF1 gene. In doing so, we have detected a missense c.353C>T polymorphism causing a proline to leucine substitution at position 118 (P118L). An association analysis with milk composition traits recorded in Murciano-Granadina goats only revealed a statistical tendency linking SREBF1 genotype and milk omega-3 fatty acid content. The lack of significant associations suggests that the P118L substitution does not involve a functional change.

scholarly journals Annatto seeds as Antioxidants Source with Linseed Oil for Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1465
Author(s):  
Jesus A. C. Osorio ◽  
João L. P. Daniel ◽  
Jakeline F. Cabral ◽  
Kleves V. Almeida ◽  
Karoline L. Guimarães ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Antioxidant Capacity ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Latin Square ◽  
Milk Composition ◽  
Chain Fatty Acid

This study aimed to evaluate the effects of annatto seeds, linseed oil and their combination on DMI, apparent total tract digestibility, antioxidant capacity and milk composition of dairy cows. Four lactating Holstein cows (120 ± 43 days in milk; 15.98 ± 2.02 kg of milk/day, mean ± SD) were allocated in a 4 × 4 Latin square with a 2 × 2 factorial arrangement (with or without annatto seeds at 15 g/kg of dry matter (DM); with or without linseed oil at 30 g/kg of DM) and provided four different diets: control (no annatto seeds or linseed oil); annatto seeds (15 g/kg of DM); linseed oil (30 g/kg of DM); and a combination of both annatto seeds and linseed oil. Annatto seeds reduced DM intake, and milk yield, protein and lactose, but increased content of fat, total solids and short chain fatty acid, with no effect on total antioxidant capacity of milk. Linseed oil supplementation decreased medium chain fatty acid proportion and n-6/n-3 ratio, conversely it increased long chain fatty acids and n-3 fatty acid content of milk, ether extract intake and total-tract digestibility. Thus, linseed oil supplementation in dairy cow diets improved the milk FA profile but decreased milk fat concentration, whereas annatto seeds did not influence antioxidant capacity and depressed feed intake and milk yield.

scholarly journals Molecular Cloning, Characterization, and Expression Regulation of Acyl-CoA Synthetase 6 Gene and Promoter in Common Carp Cyprinus carpio

2020 ◽  
Vol 21 (13) ◽  
pp. 4736
Author(s):  
Dizhi Xie ◽  
Zijie He ◽  
Yewei Dong ◽  
Zhiyuan Gong ◽  
Guoxing Nie ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Binding Protein ◽  
Core Promoter ◽  
Regulatory Element ◽  
Omega 3 ◽  
Coding Region ◽  
Positive Effects ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Omega-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA), particularly docosahexaenoic acids (22:6n-3, DHA), have positive effects on multiple biologic and pathologic processes. Fish are the major dietary source of n-3 LC-PUFA for humans. Growing evidence supports acyl-coenzyme A (acyl-CoA) synthetase 6 (acsl6) being involved in cellular DHA uptake and lipogenesis in mammals, while its molecular function and regulatory mechanism remain unknown in fish. The present study focused on investigating the molecular characterization and transcription regulation of the acsl6 gene in the freshwater teleost common carp (Cyprinus carpio). First, the full length of acsl6 cDNA contained a coding region of 2148 bp for 715 amino acids, which possessed all characteristic features of the acyl-CoA synthetase (ACSL) family. Its mRNA expression was the highest in the brain, followed by in the heart, liver, kidney, muscle, and eyes, but little expression was detected in the ovary and gills. Additionally, a candidate acsl6 promoter region of 2058 bp was cloned, and the sequence from −758 bp to −198 bp was determined as core a promoter by equal progressive deletion and electrophoretic mobility shift assay. The binding sites for important transcription factors (TFs), including stimulatory protein 1 (SP1), CCAAT enhancer-binding protein (C/EBPα), sterol-regulatory element binding protein 1c (SREBP1c), peroxisome proliferator activated receptor α (PPARα), and PPARγ were identified in the core promoter by site-directed mutation and functional assays. Furthermore, the intraperitoneal injection of PPARγ agonists (balaglitazone) increased the expression of acsl6 mRNA, coupling with an increased proportion of DHA in the muscle, while opposite results were obtained in the injection of the SREBP1c antagonist (betulin). However, the expression of acsl6 and DHA content in muscle were largely unchanged by PPARα agonist (fenofibrate) treatment. These results indicated that acsl6 may play an important role for the muscular DHA uptake and deposition in common carp, and PPARγ and SREBP-1c are the potential TFs involved in the transcriptional regulation of acsl6 gene. To our knowledge, this is the first report of the characterization of acsl6 gene and its promoter in teleosts.

scholarly journals Enhancing the DHA content in milk from dairy cows by feeding ALL-G-RICH™

2017 ◽  
Vol 5 ◽  
Author(s):  
C. A. Moran ◽  
M. Morlacchini ◽  
G. Fusconi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Milk Yield ◽  
Milk Fat ◽  
Dry Matter ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Dry Matter Intake ◽  
Human Consumption ◽  
Omega 3

SummaryThe objective of this study was to evaluate the effect of the dietary inclusion of 6 g/kg dry matter intake of an unextracted Aurantiochytrium limacinum algae (AURA) in mid-lactation Italian Friesian cows under commercial conditions on milk yield, milk composition and docosahexaenoic acid (DHA) content. Cows were allocated to two groups (n = 18; 108.2 ± 66.1 and 104.4 ± 54.6 days in milk, control and treated groups, respectively). Feeding AURA for 84 d had no effect on dry matter intake, body condition score or weight gain, but did improve milk yield by 1.9 kg/cow/d (+5.4%; P < 0.1) over the course of the experiment. Milk fat concentration declined by 12% (P < 0.0001) without any significant change in 4% fat corrected milk, protein or lactose. Supplementing AURA for 12 weeks substantially altered the fatty acid profile of milk compared with milk from CON-fed cows such that the proportion of unsaturated fatty acids increased, omega-3 fatty acid content increased by 73.1% (P < 0.0001) and was accompanied by a favourable increase in the omega-3:6 fatty acid ratio by 75.0% (P < 0.0001). The AURA supplement, during day 7–84, increased the DHA concentration to 0.37 g /100 g milk total fatty acids (P < 0.0001) with a mean transfer efficiency of 18.1% from feed to milk. Together these results indicated that supplementing a dairy cow diet with DHA-rich microalgae is a feasible and efficient means for creating DHA-enriched milk for human consumption.

Effect of Herbal Galactogogue (Sanjivani biokseera) on Milk Yield and Milk Constituents in Lactating Kankrej Cattle at Organised Farm

2019 ◽  
Vol 15 (02) ◽  
pp. 39-41
Author(s):  
H H Panchasara ◽  
A B Chaudhari ◽  
D A Patel ◽  
Y M Gami ◽  
M P Patel
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Composition ◽  
Nutritional Requirements ◽  
Not Given ◽  
Distinct Effect ◽  
Lactating Dairy Cows ◽  
Group I ◽  
Effect Of Feeding

The study was conducted to evaluate the effect of feeding herbal galactogogue preparation (Sanjivani biokseera) on the milk yield and milk constituents in lactating Kankrej cows. Thirty-two lactating Kankrej cows in their 1st to 6th lactation were taken for the experiment from 3 days after calving up to 52 days postpartum. All the animals were fed as per the standard seasonally available roughages and concentrates to meet their nutritional requirements. The cows were randomly divided into two uniform groups of 16 cows in each according to initial milk yield and milk composition. The animals in group-I were not given any supplement and served as control. The animals in group-II were given Sanjivani biokseera (Naturewell Industries) @ 60 g per day for 1-month, commencing 3 days after calving, in addition to the usual feeds/fodders. A clear difference was observed in milk yield from day 8 onward of experiment between groups with significant (plessthan0 0.05) higher values from day 16-52 in cows fed herbal galactogogue as compared to control, but no such distinct effect on milk constituents was observed on day 52 when analyzed. The use of herbal galactogogue significantly (p lessthan 0.05) increased the overall average of 52 days milk production, which was 9.34 ± 0.21 lit/day in supplemented as compared to 7.75 ± 0.26 lit/day in control animals. It was concluded that herbal galactogogue (Sanjivani biokseera) could increase milk yield in lactating dairy cows through its galactopoetic property and improved rumen environment.

scholarly journals Energy Balance Indicators during the Transition Period and Early Lactation of Purebred Holstein and Simmental Cows and Their Crosses

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 309
Author(s):  
Deise Aline Knob ◽  
André Thaler Neto ◽  
Helen Schweizer ◽  
Anna C. Weigand ◽  
Roberto Kappes ◽  
...  
Keyword(s):  
Energy Balance ◽  
Milk Yield ◽  
Milk Composition ◽  
Negative Energy ◽  
The Other ◽  
Negative Energy Balance ◽  
Metabolic Energy ◽  
Genetic Groups ◽  
Significant Difference ◽  
Fat Thickness

Crossbreeding in dairy cattle has been used to improve functional traits, milk composition, and efficiency of Holstein herds. The objective of the study was to compare indicators of the metabolic energy balance, nonesterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), glucose, body condition score (BCS) back fat thickness (BFT), as well as milk yield and milk composition of Holstein and Simmental cows, and their crosses from the prepartum period until the 100th day of lactation at the Livestock Center of the Ludwig Maximilians University (Munich, Germany). In total, 164 cows formed five genetic groups according to their theoretic proportion of Holstein and Simmental genes as follows: Holstein (100% Holstein; n = 9), R1-Hol (51–99% Holstein; n = 30), first generation (F1) crossbreds (50% Holstein, 50% Simmental; n = 17), R1-Sim (1–49% Holstein; n = 81) and Simmental (100% Simmental; n = 27). The study took place between April 2018 and August 2019. BCS, BFT blood parameters, such as BHBA, glucose, and NEFA were recorded weekly. A mixed model analysis with fixed effects breed, week (relative to calving), the interaction of breed and week, parity, calving year, calving season, milking season, and the repeated measure effect of cow was used. BCS increased with the Simmental proportion. All genetic groups lost BCS and BFT after calving. Simmental cows showed lower NEFA values. BHBA and glucose did not differ among genetic groups, but they differed depending on the week relative to calving. Simmental and R1-Sim cows showed a smaller effect than the other genetic groups regarding changes in body weight, BCS, or back fat thickness after a period of a negative energy balance after calving. There was no significant difference for milk yield among genetic groups, although Simmental cows showed a lower milk yield after the third week after calving. Generally, Simmental and R1-Simmental cows seemed to deal better with a negative energy balance after calving than purebred Holstein and the other crossbred lines. Based on a positive heterosis effect of 10.06% for energy corrected milk (ECM), the F1, however, was the most efficient crossbred line.

scholarly journals Growth, Carcass and Meat Characteristics of Grass-Fed Lambs Weaned from Extensive Rangeland and Grazed on Permanent Pastures or Alfalfa

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 52
Author(s):  
Felipe Elizalde ◽  
Christian Hepp ◽  
Camila Reyes ◽  
Marilyn Tapia ◽  
Raúl Lira ◽  
...  
Keyword(s):  
Shear Force ◽  
Nutritive Value ◽  
Production Systems ◽  
Fatty Acid Content ◽  
Saturated Fat ◽  
Growth Patterns ◽  
Omega 3 ◽  
Permanent Pasture ◽  
Lower Shear ◽  
Low Levels

Western Patagonia lamb production systems are based on extensive rangeland. The harsh climate limits the adoption of more intensive systems. Therefore, producers must focus on developing differentiated products. The aim of this study was to evaluate growth patterns, carcasses and nutritive value of meat from 45 lambs. Lambs were divided into three groups: 15 lambs were slaughtered at weaning (W), with the remaining 30 weaned lambs being allocated to grazing either alfalfa (AG) or permanent pasture (PPG). AG lambs were significantly heavier and had higher condition scores than PPG lambs. Further, AG lambs showed higher carcass weight and larger tissue depth and commercial cuts. Moreover, W lambs had lower shear force and more tender meat than either AG or PPG lambs. The three systems showed a low shear force and acceptable sensory traits. Low levels of cholesterol, with low levels of saturated fat, classified these cuts of meat as extra lean. W lambs had higher omega-3 fatty acid content than AG or PPG lambs. Overall, results showed that meat from the three lamb production systems showed health claimable levels of omega-3 fatty acids and were low in fat and thereby, can be classified as lean meat.

scholarly journals Microalgae as Sustainable Bio-Factories of Healthy Lipids: Evaluating Fatty Acid Content and Antioxidant Activity

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 357
Author(s):  
Tiago A. Conde ◽  
Bruna F. Neves ◽  
Daniela Couto ◽  
Tânia Melo ◽  
Bruno Neves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Fatty Acid Content ◽  
Scenedesmus Obliquus ◽  
Food Sources ◽  
Gas Chromatography Mass Spectrometry ◽  
Nannochloropsis Oceanica ◽  
Omega 3 ◽  
Bioactive Lipids ◽  
Food Ingredients

The demand for sustainable and environmentally friendly food sources and food ingredients is increasing, and microalgae are promoted as a sustainable source of essential and bioactive lipids, with high levels of omega-3 fatty acids (ω-3 FA), comparable to those of fish. However, most FA screening studies on algae are scattered or use different methodologies, preventing a true comparison of its content between microalgae. In this work, we used gas-chromatography mass-spectrometry (GC-MS) to characterize the FA profile of seven different commercial microalgae with biotechnological applications (Chlorella vulgaris, Chlorococcum amblystomatis, Scenedesmus obliquus, Tetraselmis chui, Phaeodactylum tricornutum, Spirulina sp., and Nannochloropsis oceanica). Screening for antioxidant activity was also performed to understand the relationship between FA profile and bioactivity. Microalgae exhibited specific FA profiles with a different composition, namely in the ω-3 FA profile, but with species of the same phylum showing similar tendencies. The different lipid extracts showed similar antioxidant activities, but with a low activity of the extracts of Nannochloropsis oceanica. Overall, this study provides a direct comparison of FA profiles between microalgae species, supporting the role of these species as alternative, sustainable, and healthy sources of essential lipids.

scholarly journals Establishment of a Patient-Derived Xenograft Model of Colorectal Cancer in CIEA NOG Mice and Exploring Smartfish Liquid Diet as a Source of Omega-3 Fatty Acids

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 282
Author(s):  
Helle Samdal ◽  
Lene C Olsen ◽  
Knut S Grøn ◽  
Elin S Røyset ◽  
Therese S Høiem ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Content ◽  
Xenograft Model ◽  
Liquid Diet ◽  
Lymphoid Cells ◽  
Omega 3 ◽  
Pdx Model ◽  
Nog Mice

Cancer patient-derived xenografts (PDXs) better preserve tumor characteristics and microenvironment than traditional cancer cell line derived xenografts and are becoming a valuable model in translational cancer research and personalized medicine. We have established a PDX model for colorectal cancer (CRC) in CIEA NOG mice with a 50% engraftment rate. Tumor fragments from patients with CRC (n = 5) were engrafted in four mice per tumor (n = 20). Mice with established PDXs received a liquid diet enriched with fish oil or placebo, and fatty acid profiling was performed to measure fatty acid content in whole blood. Moreover, a biobank consisting of tissue and blood samples from patients was established. Histology, immunohistochemistry and in situ hybridization procedures were used for staining of tumor and xenograft tissue slides. Results demonstrate that key histological characteristics of the patients’ tumors were retained in the established PDXs, and the liquid diets were consumed as intended by the mice. Some of the older mice developed lymphomas that originated from human Ki67+, CD45+, and EBV+ lymphoid cells. We present a detailed description of the process and methodology, as well as possible issues that may arise, to refine the method and improve PDX engraftment rate for future studies. The established PDX model for CRC can be used for exploring different cancer treatment regimes, and liquid diets enriched with fish oil may be successfully delivered to the mice through the drinking flasks.

Sign in / Sign up

Export Citation Format

Share Document